• Journal of Life Science
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• v.27 no.3
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• pp.370-381
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• 2017

Protein dephosphorylation is important for cellular regulation, which is catalyzed by protein phosphatases. Among protein phosphatases, carboxy-terminal domain (CTD) phosphatases are recently emerging and new functional roles of them have been revealed. There are 7 CTD phosphatases in human genome, which are composed of CTD phosphatase 1 (CTDP1), CTD small phosphatase 1 (CTDSP1), CTD small phosphatase 2 (CTDSP2), CTD small phosphatase-like (CTDSPL), CTD small phosphatase-like 2 (CTDSPL2), CTD nuclear envelope phosphatase (CTDNEP1), and ubiquitin-like domain containing CTD phosphatase 1 (UBLCP1). CTDP1 dephosphorylates the second phosphor-serine of CTD of RNA polymerase II (RNAPII), while CTDSP1, STDSP2, and CTDSPL dephosphorylate the fifth phosphor-serine of CTD of RNAPII. In addition, CTDSP1 dephosphorylates new substrates such as mothers against decapentaplegic homologs (SMADs), cell division cycle-associated protein 3 (CDCA3), Twist1, tumor-suppressor protein promyelocytic leukemia (PML), and c-Myc. CTDP1 is related to RNA polymerase II complex recycling, mitosis regulation and cancer cell growth. CTDSP1, CTDSP2 and CTDSPL are related to transcription factor recruitment, tumor suppressor function and stem cell differentiation. CTDNEP1 dephosphorylates LIPIN1 and is related to neural tube formation and nuclear envelope formation. CTDSPL2 is related to hematopoietic stem cell differentiation. UBLCP1 dephosphorylates 26S proteasome and is related to nuclear proteasome regulation. In conclusion, noble roles of CTD phosphatases are emerging through recent researches and this review is intended to summarize emerging roles of CTD phosphatases.

• Journal of the Korea Safety Management & Science
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• v.3 no.4
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• pp.77-90
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• 2001

This paper summarized the results of previous and recent studies on cumulative trauma disorders (CTD) and provides a prevention strategy for CTD and showed the results from a quite extensive survey with tables and figures. This is a follow-up study of Kwon (1993) 〔2〕. From the previous studies, Kwon (1993) showed that many people are exposed on CTD, but they did not only know the clear reasons why they got CTD, but also how to prevent or change their workstation〔2〕. Lee (1996) showed the result from both field survey and medical examination. He found that most frequent symptoms are CTS (nerve disorders) and tendon disorders 〔6〕. From these previous results, it is obvious that CTD patients will increase in Korean industries if we did not pay attention to this CTD problem. Therefore a proper education of Ergonomics, CTD prevention for workers and moderate changes in workplace are necessary. From 1998 and 1999 follow-up studies, there are still many people(23％) did not hear about CTD, CTS, or MSD. Only 42％ of respondents is exercising during or after work for short-time. Therefore, there are many rooms for prevention strategy of CTD and education for preventing CTD.

• Journal of Yeungnam Medical Science
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• v.36 no.1
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• pp.8-15
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• 2019

Connective tissue diseases (CTDs) can affect all compartments of the lungs, including airways, alveoli, interstitium, vessels, and pleura. CTD-associated lung diseases (CTD-LDs) may present as diffuse lung disease or as focal lesions, and there is significant heterogeneity between the individual CTDs in their clinical and pathological manifestations. CTD-LDs may presage the clinical diagnosis a primary CTD, or it may develop in the context of an established CTD diagnosis. CTD-LDs reveal acute, chronic or mixed pattern of lung and pleural manifestations. Histopathological findings of diverse morphological changes can be present in CTD-LDs airway lesions (chronic bronchitis/bronchiolitis, follicular bronchiolitis, etc.), interstitial lung diseases (nonspecific interstitial pneumonia/fibrosis, usual interstitial pneumonia, lymphocytic interstitial pneumonia, diffuse alveolar damage, and organizing pneumonia), pleural changes (acute fibrinous or chronic fibrous pleuritis), and vascular changes (vasculitis, capillaritis, pulmonary hemorrhage, etc.). CTD patients can be exposed to various infectious diseases when taking immunosuppressive drugs. Histopathological patterns of CTD-LDs are generally nonspecific, and other diseases that can cause similar lesions in the lungs must be considered before the diagnosis of CTD-LDs. A multidisciplinary team involving pathologists, clinicians, and radiologists can adequately make a proper diagnosis of CTD-LDs.

• Journal of Microbiology
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• v.43 no.6
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• pp.516-522
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• 2005

The phosphorylation of C-terminal domain (CTD) of Rpb1p, the largest subunit of RNA polymerase II plays an important role in transcription and the coupling of various cellular events to transcription. In this study, its role in DNA damage response is closely examined in Saccharomyces cerevisiae, focusing specifically on several transcription factors that mediate or respond to the phosphorylation of the CTD. CTDK-1, the pol II CTD kinase, FCP1, the CTD phosphatase, ESS1, the CTD phosphorylation dependent cis-trans isomerase, and RSP5, the phosphorylation dependent pol II ubiquitinating enzyme, were chosen for the study. We determined that the CTD phosphorylation of CTD, which occurred predominantly at serine 2 within a heptapeptide repeat, was enhanced in response to a variety of sources of DNA damage. This modification was shown to be mediated by CTDK-1. Although mutations in ESS1 or FCP1 caused cells to become quite sensitive to DNA damage, the characteristic pattern of CTD phosphorylation remained unaltered, thereby implying that ESS1 and FCP1 play roles downstream of CTD phosphorylation in response to DNA damage. Our data suggest that the location or extent of CTD phosphorylation might be altered in response to DNA damage, and that the modified CTD, ESS1, and FCP1 all contribute to cellular survival in such conditions.

• Tuberculosis and Respiratory Diseases
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• v.82 no.4
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• pp.285-297
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• 2019

Connective tissue disease (CTD) is a collection of disorders characterized by various signs and symptoms such as circulation of autoantibodies in the entire system causing damage to internal organs. Interstitial lung disease (ILD) which is associated with CTD is referred to as CTD-ILD. Patients diagnosed with ILD should be thoroughly examined for the cooccurrence of CTD, since the treatment procedures and prognosis of CTD-ILD are vary from those of idiopathic interstitial pneumonia. The representative types of CTD which may accompany ILD include rheumatoid arthritis, systemic sclerosis (SSc), Sjogren's syndrome, mixed CTD, idiopathic inflammatory myopathies, and systemic lupus erythematous. Of these, ILD most frequently co-exists with SSc. If an ILD is observed in the chest, high resolution computed tomography and specific diagnostic criteria for any type of CTD are met, then a diagnosis of CTD-ILD is made. It is challenging to conduct a properly designed randomized study on CTD-ILD, due to low incidence. Therefore, CTD-ILD treatment approach is yet to been established in absence of randomized controlled clinical trials, with the exception of SSc-ILD. When a patient is presented with acute CTD-ILD or if symptoms occur due to progression of the disease, steroid and immunosuppressive therapy are generally considered.

• Molecules and Cells
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• v.39 no.12
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• pp.869-876
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• 2016

Cantharidin (CTD) is an active compound isolated from the traditional Chinese medicine blister beetle and displayed anticancer properties against various types of cancer cells. However, little is known about its effect on human chronic myeloid leukemia (CML) cells, including imatinib-resistant CML cells. The objective of this study was to investigate whether CTD could overcome imatinib resistance in imatinib-resistant CML cells and to explore the possible underlying mechanisms associated with the effect. Our results showed that CTD strongly inhibited the growth of both imatinib-sensitive and imatinib-resistant CML cells. CTD induced cell cycle arrest at mitotic phase and triggered DNA damage in CML cells. The ATM/ATR inhibitor CGK733 abrogated CTD-induced mitotic arrest but promoted the cytotoxic effects of CTD. In addition, we demonstrated that CTD downregulated the expression of the BCR-ABL protein and suppressed its downstream signal transduction. Real-time quantitative PCR revealed that CTD inhibited BCR-ABL at transcriptional level. Knockdown of BCR-ABL increased the cell-killing effects of CTD in K562 cells. These findings indicated that CTD overcomes imatinib resistance through depletion of BCR-ABL. Taken together, CTD is an important new candidate agent for CML therapy.

• Ocean and Polar Research
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• v.31 no.4
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• pp.339-347
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• 2009

It is possible to obtain accurate temperature and salinity profiles of the oceans using a SBE 911plus CTD and accompanying data conversion packages. To obtain highly accurate results, CTD data needs to be carefully processed in addition to proper and regular maintenance of the CTD itself. Since the manufacturer of the CTD provides tools that are necessary for post processing, it is possible to conduct proper processing without too much effort. Some users, however, are not familiar with all of the processes and inadvertently ignore some of these processes at the expense of data quality. To draw attention to these and other similar issues, we show how it is possible to improve data quality by utilizing a few extra processes to the standard or default data process procedures with CTD data obtained from the equatorial Eastern Pacific between 2001 and 2005, and 2007. One easy step that is often ignored in the standard data process procedure is "wild edit", which removes abnormal values from the raw data. If those abnormal values are not removed, the abnormality could spread vertically during subsequent processes and produce abnormal salinity in a range much wider than that of the raw data. To remove spikes in salinity profiles the "align CTD" procedure must be carried out not with the default values included in the data processing software but with a proper time constant. Only when "cell thermal mass" correction is conducted with optimal parameters, we can reduce the difference between upcast and downcast, and obtain results that can satisfy the nominal accuracy of the CTD.

• Journal of Korean Public Health Nursing
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• v.17 no.2
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• pp.266-277
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• 2003

In order to investigate the working conditions related to cumulative trauma disorder(CTD) and symptoms of CTD, a survey was conducted covering 305 employees who were working in three furniture manufacturing companies in Kyungin area. Data were collected by using questionnaire of the Personal Ergonomics Profiles. The results were as follows : 1. The mean score of working conditions related to CTD was 0.95. The mean score of working conditions for labor workers were significantly higher than that of clerical workers. Among 4 factors of working conditions, the higher score were shown in the working and environment factors with 1.22 and 1.18, respectively. 2. Symptom prevalence rate of CTD for workers was $81.0\%\;{;}\;82.9\%$ for labor workers and $77.0\%$ for clerical workers. As for symptom of CTD in body part, higher rate were shown in lower back with $50.2\%$, shoulder and neck with $38.4\%\;and\;36.4\%$ respectively. 3. The score of symptom severity of CTD were 3.45. Significant difference was shown in symptom severity of forearm and wrist/hand part between labor and clerical workers. As for symptom severity of CTD in body part the higher score were shown in shoulder, ankle/foot, forearm. lower leg and elbow. and the highest score for labor workers were shoulder and ankle/foot and for clerical workers was lower back. 4. As a whole, symptom of CTD for workers positively correlated to working conditions. 5. Symptom of CTD for workers seemed to be significantly influenced by working factor, sex, working duration and working area factor.

• Ocean and Polar Research
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• v.36 no.1
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• pp.77-85
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• 2014

The quality control of ocean observations data is becoming a major issue as real-time observational data and information services have increased recently. Therefore, it is necessary for oceanographic instruments to calibrate. In this paper, we first introduce the CTD calibration system and traceability. Next, CTD calibration procedures and estimation of uncertainty of measurement are described. The expanded uncertainty (k = 2) of the temperature, pressure and conductivity are 0.$0.003^{\circ}C$, $6.0{\times}10^{-5}$ and 0.006 mS/cm respectively. Finally, the excellence of CTD calibration and its measurement capability has been proven by comparing the inter-calibration result of KIOST and Sea-Bird Electronics (SBE). CTD calibration residuals are less than ${\pm}0.0001^{\circ}C$, ${\pm}0.001$ MPa, ${\pm}0.0001$ S/m for SBE 3plus temperature sensor, SBE 19plus pressure sensor and SBE 4C conductivity sensor respectively.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.19 no.40
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• pp.311-319
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• 1996

Cumulative trauma disorders (CTD) refer to a category of physical signs and symptoms due to chronic musculoskeletal injuries where the antecedents appear to be related to some aspect of repetitive work which can be found commonly in modern industries. This paper describes impacts of CTD on modern industry and summarizes recent resent research efforts and suggests an ergonomic program to prevent incident of CTD in hand-intensive industry.