• 대한원격탐사학회지
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• 제39권5_3호
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• pp.967-978
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• 2023

본 연구에서는 다양한 드론 영상 데이터셋을 효과적으로 학습하여 의미론적 분할의 정확도를 향상시키기 위한 combined segmentation network (CSN)를 제안하고 검증하였다. CSN은 세 가지 드론 데이터셋의 다양성을 고려하기 위하여 인코딩 영역의 전체를 공유하며, 디코딩 영역은 독립적으로 학습된다. CSN의 경우, 학습 시 모든 데이터셋에 대한 손실값을 고려하기 때문에 U-Net 및 pyramid scene parsing network (PSPNet)으로 단일 데이터셋을 학습할 때보다 학습 효율이 떨어졌다. 그러나 국내 자율주행 드론 영상에 CSN을 적용한 결과, CSN이 PSPNet에 비해 초기 학습 없이도 영상 내 화소를 적절한 클래스로 분류할 수 있는 것을 확인하였다. 본 연구를 통하여 CSN이 다양한 드론 영상 데이터셋을 효과적으로 학습하고 새로운 지역에 대한 객체 인식 정확성을 향상시키는 데 중요한 도구로써 활용될 수 있을 것으로 기대할 수 있다.

• 한국균학회지
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• 제41권1호
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• pp.1-8
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• 2013

Cop9 signalosome(CSN)은 최초 식물 발달 과정에서의 빛에 의한 전사 조절 과정에서의 억제 유전자로 처음 분리된 이후 이들이 다양한 진핵 생물 에서 매우 잘 보존되어 있음이 알려지게 되었다. 이들은 대부분 8개의 subunit으로 구성되며 26S proteasome lid와 eIF3와 구조적으로는 물론 기능적으로도 유사성을 보인다고 알려져 있다. 이들은 특히 Cullin-Ring ubiquitin ligases(CRL)의 구성 요소인 Cullin의 deneddylation을 매개하여 ubiquitin ligase의 활성을 조절한다고 알려져 있으며, 또한 세포 주기 및 checkpoint 조절에 관여한다고 보고되었다. 분열효모의 경우 CSN1 및 CSN2 결손 세포에서 S-phase로서의 진행이 지연됨이 관찰되었고 감마선 혹은 UV에 좀더 민감해지는 현상이 관찰되어 CSN이 checkpoint 조절에 관여한다는 것을 보여주었다. 곰팡이의 CSN 경우 구조적으로 더욱 상위 개체들의 그것과 더욱 유사한데, CSN이 생체 시계 리듬, 빛과 연관한 호르몬 생산, 곰팡이의 발달 과정 및 생식 주기를 조절함이 보고되었다. 또한 Aspergillus nidulans의 경우 상위개체에서 보여준 DNA 합성 및 손상, 세포 주기 조절에서의 기능이 알려지면서 CSN은 곰팡이 생활사에 필수적인 여러 과정들을 조절하는 중요한 인자임을 알 수 있다. 이로써 식물이나 포유동물 등에서 보고되었던 CSN의 주요 기능을 미생물에서도 대부분 공유하고 있음을 알 수 있고 이들이 CRL을 통한 주요 세포 활성 조절 연구에 좋은 툴로서 활용할 수 있음을 시사하고 있다.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 1998년도 가을 학술발표논문집 Vol.25 No.2 (2)
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• pp.3-5
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• 1998

본 연구팀에서는 기존의 기호주의 전문가 시스템의 경우 지식표현 체계가 의미구조를 반영하지 못함으로써 발생하는 경직성문제를 해결하기 위해 CSN(Connectionist Semantic Network) 모델을 제안하였다. 그러나 CSN모델은 상위개념간의 관계를 표현하기 위해 단순한 전향 신경망을 사용함으로써 상위개념간의 일반적이고 구조화된 지식표현 및 추론에 어려움이 있었다. CSN 모델의 이런 문제점을 위해 본 논문에서는 상위개념간의 일반적이고 구조화된 지식표현과 추론이 용이한 기호주의 표현 체계와 이 표현 체계 안에 효과적으로 의미구조를 반영할 수 있는 연결주의 학습 모델인 CSN을 결합한 하이브리드 구조를 제안하고, 실험을 통하여 제안된 하이브리드 구조의 타당성을 보인다.

• Asian-Australasian Journal of Animal Sciences
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• 제30권12호
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• pp.1684-1688
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• 2017

Objective: From a review of published information on genetic association studies, a meta-analysis was conducted to determine the influence of the genes ${\kappa}-casein$ (CSN3) and ${\beta}-lactoglobulin$ (LGB) on milk yield traits in Holstein, Jersey, Brown Swiss, and Fleckvieh. Methods: The GLIMMIX procedure was used to analyze milk production and percentage of protein and fat in milk. Models included the main effects and all their possible two-way interactions; not estimable effects and non-significant (p>0.05) two-way interactions were dropped from the models. The three traits analyzed used Poisson distribution and a log link function and were determined with the Interactive Data Analysis of SAS software. Least square means and multiple mean comparisons were obtained and performed for significant main effects and their interactions (p<0.0255). Results: Interaction of breed by gene showed that Holstein and Fleckvieh were the breeds on which CSN3 ($6.01%{\pm}0.19%$ and $5.98%{\pm}0.22%$), and LGB ($6.02%{\pm}0.19%$ and $5.70%{\pm}0.22%$) have the greatest influence. Interaction of breed by genotype nested in the analyzed gene indicated that Holstein and Jersey showed greater influence of the CSN3 AA genotype, $6.04%{\pm}0.22%$ and $5.59%{\pm}0.31%$ than the other genotypes, while LGB AA genotype had the largest influence on the traits analyzed, $6.05%{\pm}0.20%$ and $5.60%{\pm}0.19%$, respectively. Furthermore, interaction of type of statistical model by genotype nested in the analyzed gene indicated that CSN3 and LGB genes had similar behavior, maintaining a difference of more than 7% across analyzed genotypes. These results could indicate that both Holstein and Jersey have had lower substitution allele effect in selection programs that include CSN3 and LGB genes than Brown Swiss and Fleckvieh. Conclusion: Breed determined which genotypes had the greatest association with analyzed traits. The mixed model based in Bayesian or Ridge Regression was the best alternative to analyze CSN3 and LGB gene effects on milk yield and protein and fat percentages.

• BMB Reports
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• 제41권8호
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• pp.581-586
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• 2008

The pre-replication complex (pre-RC), including the core hexameric MCM2-7 complex, ensures that the eukaryotic genome is replicated only once per cell division cycle. In this study, we identified a rice $\underline{m}ini\underline{c}hromosome$ $\underline{m}aintenance$ (MCM) homologue (OsMCM2) that functionally complemented fission yeast MCM2 (CDC19) mutants. We found OsMCM2 transcript expression in roots, leaves, and seeds, although expression levels differed slightly among the organs. Likewise, the OsMCM2 protein was ubiquitously expressed, but it was downregulated when nutritients were limiting, indicating that MCM2 expression (and therefore cell cycle progression) requires adequate nutrition. Yeast two-hybrid and GST pull-down assays demonstrated that OsMCM2 interacted with the COP9 signalosome 5 (CSN5). Taken as a whole, our results indicated that OsMCM2 functions as a subunit of the rice MCM complex and interacts with CSN5 during developmental regulation.

• Animal Bioscience
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• 제37권3호
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• pp.509-521
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• 2024

Objective: It was shown that microRNAs (miRNAs) play an important role in milk protein synthesis. However, the post-transcriptional regulation of casein expression by exogenous miRNA (xeno-miRNAs) in ruminants remains unclear. This study explores the regulatory roles of alfalfa xeno-miR162 on casein synthesis in bovine mammary epithelial cells (bMECs). Methods: The effects of alfalfa xenomiR-162 and G protein subunit gamma 11 (GNG11) on proliferation and milk protein metabolism of bMECs were detected by 5-Ethynyl-2'-Deoxyuridine (EdU) staining, flow cytometry, cell counting kit-8 (CCK-8), enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction (qRT-PCR), and Western blot. Dual-luciferase reporter assay was used to verify the targeting relationship between GNG11 and xenomiR-162. Results: Results showed that over-expression of xenomiR-162 inhibited cell proliferation but promoted apoptosis, which also up-regulated the expression of several casein coding genes, including CSN1S1, CSN1S2, and CSN3, while decreasing the expression of CSN2. Furthermore, the targeting relationship between GNG11 and xenomiR-162 was determined, and it was confirmed that GNG11 silencing also inhibited cell proliferation but promoted apoptosis and reduced the expression of casein coding genes and genes related to the mammalian target of rapamycin (mTOR) pathway. Conclusion: Alfalfa xenomiR-162 appears to regulate bMECs proliferation and milk protein synthesis via GNG11 in the mTOR pathway, suggesting that this xeno-miRNA could be harnessed to modulate CSN3 expression in dairy cows, and increase κ-casein contents in milk.

• Asian-Australasian Journal of Animal Sciences
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• 제27권3호
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• pp.414-421
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• 2014

This study was conducted to determine the effects of saturated long-chain fatty acids (LCFA) on cell proliferation and triacylglycerol (TAG) content, as well as mRNA expression of ${\alpha}s1$-casein (CSN1S1) and genes associated with lipid and protein synthesis in bovine mammary epithelial cells (BMECs). Primary cells were isolated from the mammary glands of Holstein dairy cows, and were passaged twice. Then cells were cultured with different levels of palmitate or stearate (0, 200, 300, 400, 500, and 600 ${\mu}M$) for 48 h and fetal bovine serum in the culture solution was replaced with fatty acid-free BSA (1 g/L). The results showed that cell proliferation tended to be increased quadratically with increasing addition of stearate. Treatments with palmitate or stearate induced an increase in TAG contents at 0 to 600 ${\mu}M$ in a concentration-dependent manner, and the addition of 600 ${\mu}M$ was less effective in improving TAG accumulation. The expression of acetyl-coenzyme A carboxylase alpha, fatty acid synthase and fatty acid-binding protein 3 was inhibited when palmitate or stearate were added in culture medium, whereas cluster of differentiation 36 and CSN1S1 mRNA abundance was increased in a concentration-dependent manner. The mRNA expressions of peroxisome proliferator-activated receptor gamma, mammalian target of rapamycin and signal transducer and activator of transcription 5 with palmitate or stearate had no significant differences relative to the control. These results implied that certain concentrations of saturated LCFA could stimulate cell proliferation and the accumulation of TAG, whereas a reduction may occur with the addition of an overdose of saturated LCFA. Saturated LCFA could up-regulate CSN1S1 mRNA abundance, but further studies are necessary to elucidate the mechanism for regulating milk fat and protein synthesis.

• 한국미생물·생명공학회지
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• 제32권1호
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• pp.16-21
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• 2004

Chitosanase분비 세균을 찾아내기 위해 남해안의 서로 다른 다섯 치역의 해안 갯벌과 게를 채취하였다. 시료를 키토산선별 배지에 도말하여 얻은 균주 중에 투명환을 형성하는 6종의 균주를 선택하여 분리하였다. 그들은 FE-SEM을 이용한 형태 관찰과 165 rDNA sequence analysis를 통해 Bacillus cereus KNUC51, B. cereus KNUC52, B. cereus KNUC53, B. cereus KNUC54, B. cereus KNUC55, Paenibacillus favisporus KNUC56 등으로 균주명이 정해졌다. Chitosnase 활성을 측정한 결과 기존에 알려진 B. subtilis 168과 유사한 활성을 나타내었다. 효소 활성을 높이기 위해 강력한 돌연변이 유발 물질인 MNNG를 사용하여 돌연변이주를 만든 결과 원균주와 비교해 효소활성이 높은 3개 균주를 선별할 수 있었다. B. cereus 5균주의 chitosnase를 지정하며 생산하는 csn유전자를 분리 정제하여 DNA염기서열을 결정하고 아미노산 서열을 예상하였다. 예상된 아미노산의 잔기는 453 잔기였고 B. cereus ATCC14579의 것과 93% 이상의 상동성을 나타내었다. 분리 균주의 배양 상등액을 키토산 중합체와 반응시킨 후 반응물로 박층크로 마토그래피를 실시한 결과 5분 이하로 반응을 시켰을 때 효능이 좋은 3-10개 사이의 잔기를 가진 키토산 올리고당을 만들 수 있다는 것을 볼 수 있었다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제14권6호
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• pp.2709-2734
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• 2020

The growing demand to make wireless data services 5G compatible has necessitated the development of an energy-efficient approach for an effective new wireless environment. In this paper, we first propose a cognitive sensor node (CSN) based game theory for deriving energy via a primary user-transmitted radio frequency signal. Cognitive users' time was segmented into three phases based on a time switching protocol: energy harvest, spectrum sensing and data transmission. The proposed model chooses the optimal energy-harvesting phase as the effected factor. We further propose a distributed energy-harvesting model as a utility function via pricing techniques. The model is a non-cooperative game where players can increase their net benefit in a selfish manner. Here, the price is described as a function pertaining to transmit power, which proves that the proposed energy harvest game includes Nash Equilibrium and is also unique. The best response algorithm is used to achieve the green connection between players. As a result, the results obtained from the proposed model and algorithm show the advantages as well as the effectiveness of the proposed study. Moreover, energy consumption was reduced significantly (12%) compared to the benchmark algorithm because the proposed algorithm succeeded in delivering energy in micro which is much better compared to previous studies. Considering the reduction and improvement in power consumption, we could say the proposed model is suitable for the next wireless environment represented in 5G.

• Asian-Australasian Journal of Animal Sciences
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• 제20권2호
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• pp.166-171
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• 2007

The objective of this study was to assess the association of polymorphisms in ${\kappa}$-cn, ${\beta}$-lg, ${\beta}$-lg 5′ flanking region, CSN1S2, and IGFBP-3 genes with milk production traits and mastitis-related traits in Chinese Holstein. Traits analyzed were 305 day standard milk yield, protein percentage, fat percentage, the ratio of fat percentage and protein percentage, pre-somatic cell count, somatic cell count, and somatic cell score, respectively. CSN1S2 locus was uninformative because only one genotype BB was found in Chinese Holstein. Allele frequencies of A and B in IGFBP-3 gene were 0.5738 and 0.4262 in Chinese Holstein population, which was different from reported Qinchuan cattle population. The genotypes of animals at IGFBP-3 locus significantly affected 305 day standard milk yield, protein percentage, and somatic cell score. The ${\beta}$-lg genotypes had a significant effect on protein percentage and the ratio of fat percentage and protein percentage. Polymorphism in ${\beta}$-lg 5′ flanking region was associated with 305 day standard milk yield, protein percentage, fat percentage, pre-somatic cell count, and somatic cell count. No significant associations of the polymorphism in ${\kappa}$-cn gene were observed for any trait.