• 한국지반공학회논문집
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• 제22권4호
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• pp.21-30
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• 2006

본 연구에서는 조립질 말뚝으로 개량된 점토지반의 지지력, 응력분담비, 말뚝 및 지반의 변형형태를 파악하기 위하여 말뚝의 종류(CSCP, SCP)와 치환율(0, 20, 40, 60%)을 변화시키면서 원심모형실험을 수행하였다. 실험결과, CSCP와 SCP로 개량된 지반의 하중비는 치환율이 증가함에 따라 비례적으로 증가하였고, CSCP로 개량된 지반의 평균 지지력비가 SCP로 개량한 경우보다 $8{\sim}21%$ 정도 크게 평가되었다. CSCP로 개량된 지반의 평균 응력분담비가 SCP보다 크게 나타나 CSCP가 더 큰 응력을 부담하는 것으로 평가되었다. CSCP로 보강된 지반에서는 팽창파괴가 발생하였고, SCP로 보강된 지반에서는 팽창 및 전단파괴가 동시에 발생하였다.

• Parasites, Hosts and Diseases
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• 제55권2호
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• pp.115-120
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• 2017

Encystation mediating cyst specific cysteine proteinase (CSCP) of Acanthamoeba castellanii is expressed remarkably during encystation. However, the molecular mechanism involved in the regulation of CSCP gene expression remains unclear. In this study, we focused on epigenetic regulation of gene expression during encystation of Acanthamoeba. To evaluate methylation as a potential mechanism involved in the regulation of CSCP expression, we first investigated the correlation between promoter methylation status of CSCP gene and its expression. A 2,878 bp of promoter sequence of CSCP gene was amplified by PCR. Three CpG islands (island 1-3) were detected in this sequence using bioinformatics tools. Methylation of CpG island in trophozoites and cysts was measured by bisulfite sequence PCR. CSCP promoter methylation of CpG island 1 (1,633 bp) was found in 8.2% of trophozoites and 7.3% of cysts. Methylation of CpG island 2 (625 bp) was observed in 4.2% of trophozoites and 5.8% of cysts. Methylation of CpG island 3 (367 bp) in trophozoites and cysts was both 3.6%. These results suggest that DNA methylation system is present in CSCP gene expression of Acanthamoeba. In addition, the expression of encystation mediating CSCP is correlated with promoter CpG island 1 hypomethylation.

• 디지털융복합연구
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• 제17권6호
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• pp.439-446
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• 2019

이 연구의 목적은 뇌졸중 후 발생가능한 정서적, 사회인지 문제의 중재를 위해 작업 치료에 기초한 인지자극순환프로그램(CSCP ; Cognitive Stimulation Circulative Program)의 효과를 확인하는 것이다. 이 연구에는 12명의 뇌졸중 환자가 참여하였고 CSCP는 인지과정을 촉진시키는 방법으로 조직화되었으며 총 16회기의 순환 프로그램으로 실시되었다. 프로그램의 효과를 알아보기 위하여 우울증 척도와 관계 변화 척도, 삶의 질 평가 도구를 사용하였다. 연구결과 피험자의 우울증 척도와 역할의 물리적 및 사회적 인지 기능 능력을 평가하기 위해 사용된 관계 변화 척도는 통계적으로 유의미한 변화가 있었다(p<.05). 또한, 삶의 질 영역 중에서 신체적 역할, 사회적 기능의 항목에서 의미있는 향상이 있었다. CSCP가 뇌졸중 환자의 우울을 감소시키고 사회인지 기능을 향상시켜 삶의 질을 향상시키는 효율적인 중재 프로그램으로 사용 가능함을 입증하였다.

• Parasites, Hosts and Diseases
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• 제60권1호
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• pp.1-6
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• 2022

The encystation of Acanthamoeba leads to the development of metabolically inactive and dormant cysts from vegetative trophozoites under unfavorable conditions. These cysts are highly resistant to anti-Acanthamoeba drugs and biocides. Therefore, the inhibition of encystation would be more effective in treating Acanthamoeba infection. In our previous study, a sirtuin family protein-Acanthamoeba silent-information regulator 2-like protein (AcSir2)-was identified, and its expression was discovered to be critical for Acanthamoeba castellanii proliferation and encystation. In this study, to develop Acanthamoeba sirtuin inhibitors, we examine the effects of sirtinol, a sirtuin inhibitor, on trophozoite growth and encystation. Sirtinol inhibited A. castellanii trophozoites proliferation (IC50=61.24 µM). The encystation rate of cells treated with sirtinol significantly decreased to 39.8% (200 µM sirtinol) after 24 hr of incubation compared to controls. In AcSir2-overexpressing cells, the transcriptional level of cyst-specific cysteine protease (CSCP), an Acanthamoeba cysteine protease involved in the encysting process, was 11.6- and 88.6-fold higher at 48 and 72 hr after induction of encystation compared to control. However, sirtinol suppresses CSCP transcription, resulting that the undegraded organelles and large molecules remained in sirtinol-treated cells during encystation. These results indicated that sirtinol sufficiently inhibited trophozoite proliferation and encystation, and can be used to treat Acanthamoeba infections.

• ALGAE
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• 제18권3호
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• pp.183-190
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• 2003

Except for a group I intron in trnL-uaa occuring in eubacteria and plastids, group I introns are rarely documented in plastid genomes. Here, we report that a green alga, Caulerpa sertularioides, contains three group IA3 introns in the 16S gene (cpSSU), CS-cpSSU.i1, CS-cpSSU.i2 and CS-cpSSU.i3. Each intron has an open reading frame with LAGLIDADG motifs. CS-cpSSU.i1orf and CS-cpSSU.i3orf occur at Loop 6 in the intron secondary structure and CScpSSU. i2orf at Loop 8. CS-cpSSU.i1orf and CS-cpSSU.i2orf contain both LAGLI-DADG motifs but CS-cpSSU.i3orf has only one. CS-cpSSU.i1 and CS-cpSSU.i2 share the insetion sites and the ORFs at Loop 6 and 8 with CpSSU·1 and CpSSU·2 introns of Chlamydomonas pallidostigmatica (Chlorophyceae). In contrast, CS-cpSSU.i3, containing 28 copies of GAAATAT at Loop 6, is a novel intron found only in Caulerpa sertularioides. Possible scenarios of the evolution of the three introns and their possible use in systematic research are discussed.