• Journal of the Korean Geotechnical Society
• /
• v.22 no.4
• /
• pp.21-30
• /
• 2006

In this study, centrifuge model tests were performed to investigate the stress concentration ratio, bearing capacity and deformation modes of piles in clay ground improved by granular piles with two types of pile (CSCP, SCP) and various replacement ratios (0, 20, 40, 60%). According to the results of tests, the load ratio of ground improved by SCP and CSCP proportionally increased as replacement ratio increased. It shows that average normalized load of ground improved by CSCP is higher by about $8{\sim}21%$ than by SCP. As a result of rigid loading tests, it was evaluated that average stress concentration ratio of CSCP is higher than that of SCP. Only expansion failure occurred in CSCP, whereas SCP showed the expansion and shear failure simultaneously.

• Parasites, Hosts and Diseases
• /
• v.55 no.2
• /
• pp.115-120
• /
• 2017

Encystation mediating cyst specific cysteine proteinase (CSCP) of Acanthamoeba castellanii is expressed remarkably during encystation. However, the molecular mechanism involved in the regulation of CSCP gene expression remains unclear. In this study, we focused on epigenetic regulation of gene expression during encystation of Acanthamoeba. To evaluate methylation as a potential mechanism involved in the regulation of CSCP expression, we first investigated the correlation between promoter methylation status of CSCP gene and its expression. A 2,878 bp of promoter sequence of CSCP gene was amplified by PCR. Three CpG islands (island 1-3) were detected in this sequence using bioinformatics tools. Methylation of CpG island in trophozoites and cysts was measured by bisulfite sequence PCR. CSCP promoter methylation of CpG island 1 (1,633 bp) was found in 8.2% of trophozoites and 7.3% of cysts. Methylation of CpG island 2 (625 bp) was observed in 4.2% of trophozoites and 5.8% of cysts. Methylation of CpG island 3 (367 bp) in trophozoites and cysts was both 3.6%. These results suggest that DNA methylation system is present in CSCP gene expression of Acanthamoeba. In addition, the expression of encystation mediating CSCP is correlated with promoter CpG island 1 hypomethylation.

• Journal of Digital Convergence
• /
• v.17 no.6
• /
• pp.439-446
• /
• 2019

Poststroke depression can have a secondary effect on social cognition, and this can lead to behavioral problems. The purpose of this study was to verify the effects of a Cognitive Stimulation Circulative Program(CSCP) based on occupational therapy. The participants of this study were 12 stroke patients. The CSCP was organized in such a way to promote the cognitive process. This program were carried out as a circuit program(16 sessions). As a result of this study, the depression scale of the subjects had statistically significant benefit(p<.05). Also, the evaluation results verified partial improvement in quality of life, and the relationship change scale which was used to evaluate role of physical and social cognitive function ability had statistically significant benefit(p<.05). The above results of this study verified that the CSCP was an efficient intervention program that reduces the depression of stroke patients and improve their social cognitive function ability, thus enhancing the quality of life.

• Parasites, Hosts and Diseases
• /
• v.60 no.1
• /
• pp.1-6
• /
• 2022

The encystation of Acanthamoeba leads to the development of metabolically inactive and dormant cysts from vegetative trophozoites under unfavorable conditions. These cysts are highly resistant to anti-Acanthamoeba drugs and biocides. Therefore, the inhibition of encystation would be more effective in treating Acanthamoeba infection. In our previous study, a sirtuin family protein-Acanthamoeba silent-information regulator 2-like protein (AcSir2)-was identified, and its expression was discovered to be critical for Acanthamoeba castellanii proliferation and encystation. In this study, to develop Acanthamoeba sirtuin inhibitors, we examine the effects of sirtinol, a sirtuin inhibitor, on trophozoite growth and encystation. Sirtinol inhibited A. castellanii trophozoites proliferation (IC50=61.24 µM). The encystation rate of cells treated with sirtinol significantly decreased to 39.8% (200 µM sirtinol) after 24 hr of incubation compared to controls. In AcSir2-overexpressing cells, the transcriptional level of cyst-specific cysteine protease (CSCP), an Acanthamoeba cysteine protease involved in the encysting process, was 11.6- and 88.6-fold higher at 48 and 72 hr after induction of encystation compared to control. However, sirtinol suppresses CSCP transcription, resulting that the undegraded organelles and large molecules remained in sirtinol-treated cells during encystation. These results indicated that sirtinol sufficiently inhibited trophozoite proliferation and encystation, and can be used to treat Acanthamoeba infections.

• ALGAE
• /
• v.18 no.3
• /
• pp.183-190
• /
• 2003

Except for a group I intron in trnL-uaa occuring in eubacteria and plastids, group I introns are rarely documented in plastid genomes. Here, we report that a green alga, Caulerpa sertularioides, contains three group IA3 introns in the 16S gene (cpSSU), CS-cpSSU.i1, CS-cpSSU.i2 and CS-cpSSU.i3. Each intron has an open reading frame with LAGLIDADG motifs. CS-cpSSU.i1orf and CS-cpSSU.i3orf occur at Loop 6 in the intron secondary structure and CScpSSU. i2orf at Loop 8. CS-cpSSU.i1orf and CS-cpSSU.i2orf contain both LAGLI-DADG motifs but CS-cpSSU.i3orf has only one. CS-cpSSU.i1 and CS-cpSSU.i2 share the insetion sites and the ORFs at Loop 6 and 8 with CpSSU·1 and CpSSU·2 introns of Chlamydomonas pallidostigmatica (Chlorophyceae). In contrast, CS-cpSSU.i3, containing 28 copies of GAAATAT at Loop 6, is a novel intron found only in Caulerpa sertularioides. Possible scenarios of the evolution of the three introns and their possible use in systematic research are discussed.