• Title/Summary/Keyword: CS model

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Establishment of the expression system of human HtrA2 in the zebrafish (Zebrafish 동물모델에서 human HtrA2의 expression system 정립에 관한 연구)

  • Cho, Sung-Won;Park, Hyo-Jin;Kim, Goo-Young;Nam, Min-Kyung;Kim, Ho-Young;Ko, In-Ho;Kim, Cheol-Hee;Rhim, Hyang-Shuk
    • Journal of Life Science
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    • v.16 no.4
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    • pp.571-578
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    • 2006
  • HtrA2/Omi, a mitochondrial trypsin-like serine protease, is pivotal in regulating apoptotic cell death. Several lines of recent evidence suggest that HtrA2 is associated with the pathogenesis of neurodegenerative disorders; however, the physiological function of HtrA2 still remains elusive. For studying physiological function of HtrA2 in depth, it is necessary to develop a suitable expression system in the model animal. We therefore utilized the zebrafish as a model animal to establish expression of human HtrA2 (hHtrA2) in vivo. For expression of mature HtrA2 as GFP fusion in zebrafish embryos, the HtrA2 (WT) or (S306A) cDNAs with the C-terminal GFP tag were inserted into the pCS2+ plasmid. Expression patterns of HtrA2 in HEK293 cells were first monitored by immunofluorescence staining and immunoblot assays, showing approximately 64 kDa of the HtrA2-GFP fusion proteins. Subsequently, the hHtrA2 plasmid DNA or in vitro transcribed mRNA was microinjected into zebrafish embryos. The expression patterns of HtrA2 in Zebrafish embryos were monitored by GFP fluorescence in 24 hours-post-fertilization (hpf). Although expression patterns of HtrA2-GFP in developing embryos were different between the injected DNA and mRNA, both nucleic acids revealed good expression levels to further study the physiological role of HtrA2 in vivo. This study provides a suitable condition for expressing hHtrA2 in the zebrafish embryos as well as a method for generating useful system to investigate physiological properties of the specific human genes.

Comparison of the Correction Methods for Gamma Ray Attenuation in the Radioactive Waste Drum Assay (방사성폐기물드럼 핵종분석에서 감마선 감쇠보정 방법들의 비교 평가)

  • Ji Young-Yong;Ryu Young-Gerl;Kwak Kyoung-Kil;Kang Duck-Won;Kim Ki-Hong
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.4 no.3
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    • pp.275-284
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    • 2006
  • In the measurement of gamma rays emitted from the nuclide in the radioactive waste drum, to analyze the nuclide concentration accurately, it is necessary to use the proper calibration standards and to correct for the attenuation of the gamma rays. Two drums having a different density were used to analyze the nuclide concentration inside the drum in this study. After carrying out the system calibration, we measured the gamma rays emitted from the standard source inside the model drum with changing the distance between the drum and the detector. The measured values were corrected with the three kinds of gamma attenuation correction methode, as a results, the error was less than 10 % in the low density drum and less than 25 % in the high density drum. The measured activity in the short distance was more accruable than in the long distance. The transmission correction for the mass attenuation showed good results(very Low error) compared to the mean density and the differential peak correction method.

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Study on Political Factors for Innovating Textile and Fashion Industry in Northern Gyeonggi Province (경기북부 섬유패션산업 혁신을 위한 필요 정책요인 분석연구)

  • Yoon, Chang-Ju;Hwang, Chan-Gyu;Kwon, Hun-Gong;Won, Moon-Ye
    • Journal of Convergence for Information Technology
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    • v.8 no.1
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    • pp.253-263
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    • 2018
  • Textile fashion industry is a core foundation industry, having the majority of companies with 10 or more workers, in Northern Gyeonggi Province. however the industry is mostly comprised of small unit-stream enterprises, orders are greatly reduced due to lately accelerated overseas expansion of medium/large-sized vendors and the growth-inhibiting vicious circle has being set in, as this situation causes the reduction of investment. For resolving the problems, this study proposes required political factors and concrete policy proposals by designing AHP research model(4 layers and 36 elements), based on grasp of the transitional aspect of industrial scale and business environment through analysis of various industrial statistics, preceding research such as related literature search and (industrial/academic/R&D/government) specialist opinion investigation, and then calculating relative importance and priority of each factor(element) within each layer. And for raising usefulness and availability of the research result by concretely suggesting the vision, strategies, core tasks and detailed projects in which the research model and deduced result are reflected.

In the Fusion Era of Jung, The Relationship between College Students' Perfectionism and Career Indecision: Focusing on Mediation Effect of Career Decision Self-Efficacy (융복합시대에 대학생들의 완벽주의와 진로미결정의 관계: 진로결정 자기효능감의 매개효과를 중심으로)

  • Park, Myoung-Sun;Park, Jong-Won
    • Journal of Convergence for Information Technology
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    • v.8 no.1
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    • pp.79-87
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    • 2018
  • The purpose of this study was to investigate the relationships among psychological, emotional variables related to the career indecision of college students and to present effective action strategies by searching basic data that can prevent college students from undeciding their career path. In this study, we analyzed the relationship between the variables related to career indecision of college students and the structural equation model to verify the fit of the research model. The results of this study suggest that adaptive perfectionism has a positive effect on career decision self-efficacy and career decision self-efficacy has a negative effect on career indecision and adaptive perfectionism has a negative effect on career indecision by mediating career decision self-efficacy. In addition, maladaptive perfectionism has a negative effect on career decision self-efficacy, and it has a positive effect on career indecision through mediation of career decision self-efficacy. we discussed the implications for career decision making and employment of college students based on the results of the relationship between variables such as perfectionism, career decision self-efficacy, and career indecision.

Bond Strength of Wafer Stack Including Inorganic and Organic Thin Films (무기 및 유기 박막을 포함하는 웨이퍼 적층 구조의 본딩 결합력)

  • Kwon, Yongchai;Seok, Jongwon
    • Korean Chemical Engineering Research
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    • v.46 no.3
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    • pp.619-625
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    • 2008
  • The effects of thermal cycling on residual stresses in both inorganic passivation/insulating layer that is deposited by plasma enhanced chemical vapor deposition (PECVD) and organic thin film that is used as a bonding adhesive are evaluated by 4 point bending method and wafer curvature method. $SiO_2/SiN_x$ and BCB (Benzocyclobutene) are used as inorganic and organic layers, respectively. A model about the effect of thermal cycling on residual stress and bond strength (Strain energy release rate), $G_c$, at the interface between inorganic thin film and organic adhesive is developed. In thermal cycling experiments conducted between $25^{\circ}C$ and either $350^{\circ}C$ or $400^{\circ}C$, $G_c$ at the interface between BCB and PECVD $ SiN_x $ decreases after the first cycle. This trend in $G_c$ agreed well with the prediction based on our model that the increase in residual tensile stress within the $SiN_x$ layer after thermal cycling leads to the decrease in $G_c$. This result is compared with that obtained for the interface between BCB and PECVD $SiO_2$, where the relaxation in residual compressive stress within the $SiO_2$ induces an increase in $G_c$. These opposite trends in $G_cs$ of the structures including either PECVD $ SiN_x $ or PECVD $SiO_2$ are caused by reactions in the hydrogen-bonded chemical structure of the PECVD layers, followed by desorption of water.

A study on vision system based on Generalized Hough Transform 2-D object recognition (Generalized Hough Transform을 이용한 이차원 물체인식 비젼 시스템 구현에 대한 연구)

  • Koo, Bon-Cheol;Park, Jin-Soo;Chien Sung-Il
    • Journal of the Korean Institute of Telematics and Electronics B
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    • v.33B no.1
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    • pp.67-78
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    • 1996
  • The purpose of this paper is object recognition even in the presence of occlusion by using generalized Hough transform(GHT). The GHT can be considered as a kind of model based object recognition algorithm and is executed in the following two stages. The first stage is to store the information of the model in the form of R-table (Reference table). The next stage is to identify the existence of the objects in the image by using the R-table. The improved GHT method is proposed for the practical vision system. First, in constructing the R-table, we extracted the partial arc from the portion of the whole object boundary, and this partial arc can be used for constructing the R-table. Also, clustering algorithm is employed for compensating an error arised by digitizing an object image. Second, an efficient method is introduced to avoid Ballard's use of 4-D array which is necessary for estimating position, orientation and scale change of an object. Only 2-D array is enough for recognizing an object. Especially, scale token method is introduced for calculating the scale change which is easily affected by camera zoom. The results of our test show that the improved hierarchical GHT method operates stably in the realistic vision situation, even in the case of object occlusion.

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Usage and Analysis on Readability of Korean Typography in WBI for Children (효과적인 아동용 WBI를 위한 한글 타이포그래피의 가해성 분석과 활용)

  • Han, Jeong-Hye;Kim, Yong-Dae
    • Journal of The Korean Association of Information Education
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    • v.6 no.3
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    • pp.328-337
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    • 2002
  • Looking at multimedia education contents from a design point of view, the instructor's design model may differ from the child's understanding model due to gap of the instructor's and child's knowledge. This fact implies it impacts the effectiveness of the education contents. The learning efficiency of Korean typography in WBI for children depends on the font-family, line space, font-size, the age of user, the output device such as the monitor, and other various factors. In this paper, we measured and analyzed on readability of Korean typography in WBI for children by reading speed method. The results of experiments show that readability depends on the font-family of typography, age(grade), and sex of children. In detail, "Goolymche" has the shortest time to be read, and girl and the highest grade students of elementary school have shorter time than boy and the lower grade students. Moreover, we consider the elegance of typography in WBI for holding children's interests because they prefer "Yopseoche". We provide some CSSs in WBI for children based on the experimental results, to used in school fields.

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Analysis regarding Complaints of Courier Consumers and Workers in the Parcel Delivery Service by using Topic Model (토픽모델을 활용한 택배 서비스 소비자와 종사자의 불만 사항 분석)

  • Shin, Jin Gyu
    • Journal of Convergence for Information Technology
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    • v.10 no.2
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    • pp.39-48
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    • 2020
  • Many studies have been conducted to analyze factors that affect customer satisfaction, and service quality improvement in the parcel delivery industry. Most of these studies have a limited number of respondents using methods such as surveys and interviews. Therefore, this study aims to supplement the shortcomings of previous studies, by searching and analyzing the common major topics related to the complaints pointed out by consumers and suppliers in the parcel delivery service with cases of consumer counseling, and articles that reflect the complaints of workers in the industry. In addition, by analyzing the trend of these topics, we attempted to discover new topics and suggest implications. In conclusion, topics such as delay/lost/wrong deliveries as well as the fierce competition in the parcel delivery industry, turned out to be central aspects. As a result of the topic trend analysis, talks with international couriers have recently increased, and many conflicts related to apartment parcel delivery have been dealt with. The topics presented in this study are mainly focused on the contents of previous studies, but we expect that new and valuable topics can be derived by adding other data and analysis methods, such as internal counseling and academic literature.

The Study on Speaker Change Verification Using SNR based weighted KL distance (SNR 기반 가중 KL 거리를 활용한 화자 변화 검증에 관한 연구)

  • Cho, Joon-Beom;Lee, Ji-eun;Lee, Kyong-Rok
    • Journal of Convergence for Information Technology
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    • v.7 no.6
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    • pp.159-166
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    • 2017
  • In this paper, we have experimented to improve the verification performance of speaker change detection on broadcast news. It is to enhance the input noisy speech and to apply the KL distance $D_s$ using the SNR-based weighting function $w_m$. The basic experimental system is the verification system of speaker change using GMM-UBM based KL distance D(Experiment 0). Experiment 1 applies the input noisy speech enhancement using MMSE Log-STSA. Experiment 2 applies the new KL distance $D_s$ to the system of Experiment 1. Experiments were conducted under the condition of 0% MDR in order to prevent missing information of speaker change. The FAR of Experiment 0 was 71.5%. The FAR of Experiment 1 was 67.3%, which was 4.2% higher than that of Experiment 0. The FAR of experiment 2 was 60.7%, which was 10.8% higher than that of experiment 0.

The Effect of Socially-Prescribed Perfectionism of College Students to Depression: Testing the Mediation effect of Intolerance of Uncertainty and Unconditional Self Acceptance (대학생의 사회부과적 완벽주의가 우울에 미치는 영향: 불확실성에 대한 인내력 부족과 무조건적 자기수용의 매개효과를 중심으로)

  • Choi, Jea-Gwang;Song, Wonyoung
    • Journal of Convergence for Information Technology
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    • v.8 no.3
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    • pp.183-191
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    • 2018
  • This study is to examine the effects of Socially Prescribed Perfectionism on depression by Intolerance of Uncertainty and Unconditional Self Acceptance, and to well being to improve the positive life of college students. This study is conducted on 238 college students who are influenced by Socially Prescribed Perfectionism, Intolerance of Uncertainty, Unconditional Self Acceptance, and Depression. This study analyzed a questionnaire consisted of a sub-component of the Multidimensional Perfectionism Scale (MPS), a Intolerance of Uncertainty Scale(IUS), an Unconditional Self Acceptance Questionnaire-R(USAQ-R), and a depression scale (CES-D) and verified correlation analysis and structural equation model. The results of this study showed that socially prescribed perfectionism had significant negative correlations with intolerance of uncertainty, and had significant positive correlation with unconditional self acceptance. The results of the structural equation model showed full mediating effect of the intolerance of uncertainty and unconditional self acceptance between Socially prescribed perfectionism and depression, Finally, implications and suggestions are suggested in this study.