• Title/Summary/Keyword: CR39

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Pregnancy and Survival Rate of In Vitro Fertilized Bovine Embryos Frozen for Direct Transfer (직접이식을 위한 소 체외 수정란의 동결 융해후 생존성 및 수태율에 미치는 영향)

  • 오성종;양보석;이명식;백광수;성환후;정진관;임경순
    • Korean Journal of Animal Reproduction
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    • v.19 no.1
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    • pp.49-54
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    • 1995
  • This experiments were carried out to investigate the viabilities and the pregnancy rate of frozen-thawed IVF bovine embryos in various media, cryoprotectants and age of embryos produced. Hanwoo oocyte were collected in size of 2~7mm follicles, matured for 20~22hrs at 38.5$^{\circ}C$ in 5% CO2 incubator and then in vitro fertilized with Hanwoo semen. Blastocysts or more developed embryos at Day 7, 8 and 9 were frozen in 1.5 or 1.8M ethylene glycol. Viability of frozen thawed IVF embryos were identified the reformation of blastocoele after thawing and culture for 24~48 hours at 38.5$^{\circ}C$ in 5% CO2 incubator. Production rate of Hanwoo IVF embryos in TCM 199 and CR1aa ws 21.3%(39/183) and 28.1%(41/146), respectively. The viability of frozen thawed IVF embryos was higher rate in 1.8M ethylene glycol and Day 7 embryos than that in 1.5M and Day 8.53 cows out of 100 Hanwoo receipients transfered IVF embryos were pregnant and twin production rate was 26.3%.

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한우 성체 귀세포의 핵이식에 의한 복제 송아지의 생산

  • 이상영;유재숙;박영호;정대석;양병철;임석기;박수봉;장원경;박춘근
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.135-135
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    • 2003
  • 본 연구는 체세포 핵이식 복제 수정란의 이식에 의한 고능력 한우를 다량 증식하기 위한 방안을 확립하기 위하여 수행되었다. 본 실험에 공여된 체세포는 육질과 육량 등급이 국내에서 100위 이내의 암소 귀세포를 채취하여 동결 및 계대배양하여 사용하였다. 한편, 핵이식 수정란의 준비를 위하여 도축장에서 채취한 난소에서 난자를 회수하여 22시간 성숙배양 후 난구세포를 제거하고 극체가 존재하는 난자만을 선별하여 recipient cytoplasm으로 이용하였다. 난자의 제핵, 체세포 핵이식, 전기융합 및 활성화 처리는 본 실험실의 방법에 준하여 실시하였으며, 핵이식란은 CR1aa 배양액 내에서 5% $CO_2$, 95% Air 및 39$^{\circ}C$의 기상조건하에서 7일간 배양 후 이식에 이용되었다. 한편, 수란축은 2회 이상 정상 발정주기가 확인된 경산우와 미경산우에 25mg의 PG $F_{2}$$\alpha$/를 투여하여 발정을 유기하거나 자연발정우를 선발하여 수란축으로 이용하였다. 그 결과, 배반포기배를 이식한 경우 14두중 5두에서 임신이 확인되었으며 그중 4두에서 유산되었고, 1두는 임신 6개월령으로 정상 발육되고 있는 것이 확인되었지만 상실배기단계에서 이식된 경우는 임신이 되지 않았다. (중략)

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Design and fabrication of condenser microphone with rigid backplate and vertical acoustic holes using DRIE and wafer bonding technology (기판접합기술을 이용한 두꺼운 백플레이트와 수직음향구멍을 갖는 정전용량형 마이크로폰의 설계와 제작)

  • Kwon, Hyu-Sang;Lee, Kwang-Cheol
    • Journal of Sensor Science and Technology
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    • v.16 no.1
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    • pp.62-67
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    • 2007
  • This paper presents a novel MEMS condenser microphone with rigid backplate to enhance acoustic characteristics. The MEMS condenser microphone consists of membrane and backplate chips which are bonded together by gold-tin (Au/Sn) eutectic solder bonding. The membrane chip has 2.5 mm${\times}$2.5 mm, $0.5{\mu}m$ thick low stress silicon nitride membrane, 2 mm${\times}$2 mm Au/Ni/Cr membrane electrode, and $3{\mu}m$ thick Au/Sn layer. The backplate chip has 2 mm${\times}$2 mm, $150{\mu}m$ thick single crystal silicon rigid backplate, 1.8 mm${\times}$1.8 mm backplate electrode, and air gap, which is fabricated by bulk micromachining and silicon deep reactive ion etching. Slots and $50-60{\mu}m$ radius circular acoustic holes to reduce air damping are also formed in the backplate chip. The fabricated microphone sensitivity is $39.8{\mu}V/Pa$ (-88 dB re. 1 V/Pa) at 1 kHz and 28 V polarization voltage. The microphone shows flat frequency response within 1 dB between 20 Hz and 5 kHz.

Geochemistry and K-Ar Age of Gabbroic Rocks in the Konamsan Area of Yonchon Province, South Korea (연천 고남산 지역에 분포하는 반려암질암의 암석화학과 관입시기)

  • Kim, Kyu Han;Lee, Hyun Joo
    • Economic and Environmental Geology
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    • v.27 no.1
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    • pp.29-39
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    • 1994
  • Gabbroic rocks in which titanomagnetite orebodies are embedded were emplaced in Precambrian metasedimentary rocks. Hornblende K-Ar ages for equigranula and gneissic gabbros were obtained to be $1021.8{\pm}14.5$ Ma and $1468.4{\pm}20.8$ Ma, respectively. Biotite-granite has an age of 116.4 Ma, which has corresponded to Daebo granite. Amphibole minerals of the gabbroic rock and the magnetite orebodies belong to calcic amphibole group such as ferroan pargasite, pargasite, and ferro-pargasite. The gabbroic rocks have unusually high content of $TiO_2$ ranging from 0.88 to 6.03 wt.% with an average value of 3.46 wt.% as compared to normal gabbroic rock with 1.32 wt.% in $TiO_2$. Incompatible elements such as Ba and Sr of the gabbros are negatively correlated with $SiO_2$. In contrast, Co and Cr have a positive correlation with $SiO_2$, suggesting a normal differentiation trend of gabbroic magma.

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Analysis of Heavy Metal Concentration on Working Clothes for Waste Incinerating Workers (생활폐기물 소각장 작업복의 중금속 분석)

  • Park, Soon-Ja
    • The Korean Journal of Community Living Science
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    • v.18 no.1
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    • pp.39-53
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    • 2007
  • The purpose of this study was to determine the characteristics of an experimental protective clothing material with regard to comfort and isolation from the hazardous heavy metals produced in municipal waste incineration. An analysis was conducted on the total concentrations of heavy metals in some parts such as surface, middle layer, and interior for the treated fabric, and the untreated one, and working clothes. We conclude that the processed fabric with charcoal for working clothes showed the least exposure to heavy metals of the three. Working clothes worn by workers during waste incineration were much more contaminated than the untreated and treated materials. The material of working clothes could be chosen according to the function with regard to its original chemical characteristics, which are the proper results of the dyeing process. The processed fabric material has high degrees of moisture regain, thermal insulation, water vapor penetration, and antibacterial function; consequently, it is much more comfortable to wear. The fabric material proposed in this research contributed much more to blocking heavy metal concentrations (such as Cd, Pb, Cu, Cr, Zn, Mn) than did the fabric of working clothes at present. Consequently, we strongly suggest that the material of working clothes be upgraded by adopting the above-mentioned charcoal-processed fabric. Materials of working clothes must be improved to increase comfort and prevent harmful gas, flying dust, and heavy metals from permeating the fabrics.

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Microstructure and Varistor Properties of ZPCCAE Ceramics with Erbium

  • Nahm, Choon-Woo;Heo, Jae-Seok;Lee, Geun-Hyung
    • Transactions on Electrical and Electronic Materials
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    • v.15 no.4
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    • pp.213-216
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    • 2014
  • The microstructure and varistor properties of ZPCCAE ($ZnO-Pr_6O_{11}-CoO-Cr_2O_3-Al_2O_3-Er_2O_3$) ceramics were investigated with different erbium amounts. Analysis of the microstructure indicated that the ceramics consisted of ZnO grains as a bulk phase, and intergranular layers (mixture of $Pr_6O_{11}$ and $Er_2O_3$) as a minor secondary phase. With the increase of the doped erbium amount, the densities of sintered pellets increased from 5.63 to $5.82g/cm^3$, and the average grain size decreased from 9.0 to $5.7{\mu}m$. The increase of the doped erbium amount increased the breakdown field from 2,649 to 5,074 V/cm, and the nonlinear coefficient from 27.6 to 39.1. It was found that in the range of 0.25 to 0.5 mol%, the doped erbium had little effect on the microstructure and electrical properties.

Design and Fabrication of MEMS Condenser Microphone Using Wafer Bonding Technology (기판접합기술을 이용한 MEMS 컨덴서 마이크로폰의 설계와 제작)

  • Kwon, Hyu-Sang;Lee, Kwang-Cheol
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.16 no.12 s.117
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    • pp.1272-1278
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    • 2006
  • This paper presents a novel MEMS condenser microphone with rigid backplate to enhance acoustic characteristics. The MEMS condenser microphone consists of membrane and backplate chips which are bonded together by gold-tin(Au/Sn) eutectic solder bonding. The membrane chip has $2.5mm{\times}2.5mm$, 0.5${\mu}m$ thick low stress silicon nitride membrane, $2mm{\times}2mm$ Au/Ni/Cr membrane electrode, and 3${\mu}m$ thick Au/Sn layer. The backplate chip has $2mm{\times}2mm$, 150${\mu}m$ thick single crystal silicon rigid backplate, $1.8mm{\times}1.8mm$ backplate electrode, and air gap, which is fabricated by bulk micromachining and silicon deep reactive ion etching. Slots and $50{\sim}60{\mu}m$ radius circular acoustic holes to reduce air damping are also formed in the backplate chip. The fabricated microphone sensitivity is 39.8 ${\mu}V/Pa$(-88 dB re. 1 V/Pa) at 1 kHz and 28 V polarization voltage. The microphone shows flat frequency response within 1 dB between 20 Hz and 5 kHz.

The Influence of Alloying Elements Addition on the Electrical and Mechanical Properties of Cu-Ni-Si-P Alloy (Cu-Ni-Si-P 합금의 기계적 및 전기적 성질에 미치는 첨가원소의 영향)

  • Kim, Seung-Ho;Yum, Young-Jin;Park, Dong-Hwan
    • Journal of the Korean Society for Heat Treatment
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    • v.27 no.1
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    • pp.1-9
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    • 2014
  • For connector material applications, the influence alloying elements of Mn, Cr, Fe, and Ti and cold rolling reduction on the mechanical property, electrical conductivity and bendiability of Cu-Ni-Si-P alloy was investigated. The hot rolled plates were solution treated at $980^{\circ}C$ for 1.5 h, quenched into water, cold rolled by 10% and 30% reduction in thickness, and then aged at $440{\sim}500^{\circ}C$ for 3, 4, 5 times. respectively. Cu-Ni-Si-P-x alloys cold rolled by 10 reduction before heat treatment have a good bendability compare to cold rolled by 30 reduction. Cu-3.4Ni-0.8Si-0.03P-0.1Ti shows the peak strength value of 759 MPa, an electrical conductivity of 39%IACS, an elongation of 10% and a hardness of 256 Hv aged at $440^{\circ}C$ for 6 hrs. Thus it is suitable for lead frame and connector.

A Photoelastic Study on Change of Stress Concentration Factor Due to Unsymmetrical Change of Notch Angle (Pure Bending Moment 하(下)에 있는 Notched Strip에서의 Notch Angle의 비대칭적(非對稱的) 변화(變化)에 의(依)한 응력집중계수(應力集中係數)의 변화(變化))

  • Jang-Chule,Mun
    • Bulletin of the Society of Naval Architects of Korea
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    • v.4 no.1
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    • pp.55-58
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    • 1967
  • It is investigated that in what shape the stress concentration factor of a notched strip under pure bending changes due to unsymmetrically varying notch angle. Four models made of CR-39, having parameters of r/d-0.225, h/r=4; r/d=0.225, h/r=2; r/d=0.4, h/r=4 and r/d=0.4, h/r=2 respectively as shown in Fig.1, Fig.2 and Table are tested with the use of polariscope. For each model, notch angle is unsymmetrically varied from $0^{\circ}$ to $180^{\circ}$ at intervals of $15^{\circ}$ as shown in Fig. 1 and Fig. 2. The results of this experiment are Fig. 7 and Fig. 8 and the following are deduced. As notch angle increases 1) from $0^{\circ}$ to $50^{\circ}$, the decrement of concentration factor is slight. 2) from $50^{\circ}$ to 90, the decrement of concentration factor is a little steeper. 3) from $30^{\circ}$ to $140^{\circ}$, the decrement of concentration factor is slight. 4) from $140^{\circ}$ to $180^{\circ}$, the decrement is very steep with an abrupt with an abrupt change in the neighborhood of $140^{\circ}$.

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The Distribution of Boundaty Stresses around the Lightening Hole in a Triangular Bracket. (Bracket의 Lightening Hole 주변(周邊)에서의 응력분포(應力分布))

  • Hyo-Chul,Kim
    • Bulletin of the Society of Naval Architects of Korea
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    • v.3 no.1
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    • pp.11-18
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    • 1966
  • In a polarized light field, triangular plate bracket specimen of CR-39 with lightening hole were subjected to tension. The variables of the models used in the experiment were taken in the range of length-depth $ratio=0.583{\sim}1.715$, eccentricity of lightening hole from the geometrical center of $bracket=-1/4"{\sim}+1/4"$, and the lightening hole $diameter=1/2"{\sim}2"$. The isoclinics were drawn and from those the stress trajectories were constructed. Then the distributions of boundary stress around the lightening holes were determined from the isochromatic fringe pattern. The conclusions reached in this investigation are as follows: 1. Maximum stresses of the hole boundary are gradually increased when the diameter of the lightning hole increase. 2. Maximum stresses of the lightning hole boundary are decreased gradually when the eccentricity of the lightning hole from the geometrical center of the bracket to the farther side from the free end. 3. If the minimum distances from the free end of the brackets to the lightening hole boundaries are equal, the variation of the maximum stresses are in a small range for the change of lightening hole diameter and its location. 4. When the length-depth ratios are smaller than 0.8, the maximum stresses increase steeply. In the range of $0.8{\sim}1.2$ maximum stresses increase gradually and thereafter increase rapidly when the length-depth ratio of the bracket increase for the same diameter of a lightening hole.

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