• Biomolecules & Therapeutics
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• v.30 no.2
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• pp.145-150
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• 2022

In this study, we investigated the influence of galangin on vascular contractibility and to determine the mechanism underlying the relaxation. Isometric contractions of denuded aortic muscles were recorded and combined with western blot analysis which was performed to measure the phosphorylation of phosphorylation-dependent inhibitory protein of myosin phosphatase (CPI-17) and myosin phosphatase targeting subunit 1 (MYPT1) and to evaluate the effect of galangin on the RhoA/ROCK/CPI-17 pathway. Galangin significantly inhibited phorbol ester-, fluoride- and thromboxane mimetic-induced vasoconstrictions regardless of endothelial nitric oxide synthesis, suggesting its direct effect on vascular smooth muscle. Galangin significantly inhibited the fluoride-dependent increase in pMYPT1 and pCPI-17 levels and phorbol 12,13-dibutyrate-dependent increase in pERK1/2 level, suggesting repression of ROCK and MEK activity and subsequent phosphorylation of MYPT1, CPI-17 and ERK1/2. Taken together, these results suggest that galangin-induced relaxation involves myosin phosphatase reactivation and calcium desensitization, which appears to be mediated by CPI-17 dephosphorylation via not PKC but ROCK inactivation.

• Biomolecules & Therapeutics
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• v.31 no.2
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• pp.193-199
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• 2023

In this investigation, we made a study of the efficacy of luteolin (a flavonoid found in plants such as vegetables, herbs and fruits) on vascular contractibility and to elucidate the mechanism underlying the relaxation. Isometric contractions of denuded muscles were stored and combined with western blot analysis which was conducted to assess the phosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) and phosphorylation-dependent inhibitory protein for myosin phosphatase (CPI-17) and to examine the effect of luteolin on the RhoA/ROCK/CPI-17 pathway. Luteolin significantly alleviated phorbol ester-, fluoride- and thromboxane mimetic-elicited contractions regardless of endothelial nitric oxide synthesis, implying its direct effect on smooth muscle. It also significantly alleviated the fluoride-elicited elevation in pCPI-17 and pMYPT1 levels and phorbol 12,13-dibutyrate-elicited increase in pERK1/2 level, suggesting depression of ROCK and PKC/MEK activity and ensuing phosphorylation of MYPT1, CPI-17 and ERK1/2. Taken together, these results suggest that luteolin-elicited relaxation includes myosin phosphatase reactivation and calcium desensitization, which seems to be arbitrated by CPI-17 dephosphorylation via ROCK/PKC inhibition.

• Journal of Gifted/Talented Education
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• v.25 no.4
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• pp.511-528
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• 2015

The development process from creative potential to realized talent is complex and non-linear. This feature of the process stands out more in the process of living a creative life in the long-term rather than in a situation to solve certain problems in the short-term. The purpose of this study is to develop Creativity Path Inventory (CPI) for undergraduate students based on Sawyer's Zigzag Model which is one of creative process theories and to verify reliability and validity of the inventory. Thus, reflecting the characteristics of each stage of the model, this study developed 88 items in 8 factors and finally confirmed 38 items in 7 factors through item analysis and verification process on construct validity. Internal consistency of a total of 38 items in CPI turned out to be .835, confirming the reliability of the inventory and goodness-of-fit index of the final model also demonstrated an appropriate result. CPI with verified reliability and validity will help enable people who want to manifest creativity in view of everyday creativity to realize self-improvement by self-reporting their strengths and weaknesses on their own.

• Korean Journal of Veterinary Service
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• v.46 no.1
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• pp.29-34
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• 2023

Mycobacterium tuberculosis complex (M. tuberculosis complex) is a causative agent of contagious chronic disease in a wide range of mammalian hosts, mainly cattle, goat, pigs, wildlife, and humans. The definite diagnosis of tuberculosis is made based on culture of M. tuberculosis, but it takes a long time. In the present study, we analyzed whether the detection time of M. tuberculosis could be reduced when cultured in the medium containing the culture-promoting ingredients-107 (CPI-107) using the BacT/Alert 3D system, an automatic culture system. The time to detection (TTD) tended to decrease as the added concentration of CPI-107 increase. In the case of low numbers of M. tuberculosis, it decreased by 21.0% at 1.2 mg/mL of CPI-107 and by 15.9% in the case of high numbers of M. tuberculosis. In the culture using clinically isolated M. tuberculosis strains, the shortening of the culture time by CPI was more evident. In conclusion, the detection time of M. tuberculosis was shortened in the medium added with CPI-107, and this could be used for isolation, culture and drug susceptibility test of M. tuberculosis.

• The korean journal of orthodontics
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• v.28 no.1 s.66
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• pp.113-122
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• 1998

Stabilization splint therapy Precedes orthodontic intervention to enable the operator to find a 'true' centric(which is stable and comfortable), to test the patient's response to a change in the occlusion, prior to embarking upon a complex course of occlusal therapy : and finally, to see if the centric relation position can be stabilized. For this study, 47 malocclusion Patients enrolled for orthodontic treatment at the Department of Orthodontics, College of Dentistry, Chosun University comprised the malocclusion group, little variation of growth factor by the second molar eruption. They had Cr-Co discrepancy beyond normal range. For each patients the stabilization splint with mutually protected type of occlusal scheme was applied for 3 months. Condylar positions in CR and CO were measured using Penadent articulators, Panadent condylar position indicator(CPI), and transcranial projection before & after stabilization splint therapy. On the basis of this study, the results of this study were as follows 1. In all samples using CPI, there were statistical significances in CR-CO discrepancy(p<0.001) both before 8t after stabilization splint therapy. 2. In Rt and Lt+Rt/2 of superior joint space using transcranial projection, there were statistical significances in CR-CO discrepancy({<0.05) before & after stabilization splint therapy. 3. In supero-inferior components using CPI, there were statistical significances in CR-CO discrepancy(p<0.01) before & after stabilization splint therapy. 4. In all components except Rt using transcranial projection, there were no statistical significances in CR-CO discrepancy(p>0.05) before & after stabilization splint therapy. To sum up, CPI might be more effective than transcranial projection to reveal the changes between CR-CO discrepancies and stabilization splint might be more useful appliance for displaying the vertical changes, than the antero-posterior changes, of condylar position.

• Journal of Microbiology and Biotechnology
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• v.20 no.8
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• pp.1243-1250
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• 2010

A cDNA encoding a cysteine protease inhibitor (CPI) was isolated from the cDNA library of clamworm Perinereis aibuhitensis Grube. The deduced amino acid sequence analysis showed that the protein had 51%, 48%, and 48% identity with Zgc:153129 from Danio rerio, cystatin B from Theromyzon tessulatum, and the ChainA, stefin B tetramer from Homo sapiens, respectively. The gene was cloned into the intracellular expression vector pET-15b and expressed in Escherichia coli. The recombinant CPI (PA-CPI) was purified by affinity chromatography on Ni-charged resin and ion-exchange chromatography on DEAE-Sepharose FF. The relative molecular mass of PA-CPI was 16 kDa as deduced by SDS-PAGE. Activity analysis showed that the recombinant protein could inhibit the proteolytic activity of papain. A constitutive and secretive expression vector was also constructed, and the cDNA encoding CPI was subcloned into the vector for extracellular expression. Western blotting analysis results showed that the PA-CPI was secreted into the medium. Bioassay demonstrated that E. coli DH5${\alpha}$ harboring pUC18ompAcat-CPI showed a significant difference in mortality to the Asian longhorned beetle Anoplophora glabripennis compared with untransformed E. coli DH5${\alpha}$ and control.

• Journal of Periodontal and Implant Science
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• v.50 no.4
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• pp.268-277
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• 2020

Purpose: Many home care treatments can be used to promote the health and longevity of dental implants; however, few studies are available to support the concept that self-performed oral hygiene behaviors are an essential tool for improving and maintaining oral health. We investigated age-stratified associations between dental health behaviors related to tooth brushing (TB) and oral hygiene product use in Korean adults with implants. Methods: A total of 1,911 subjects over 19 years of age who had 1 or more implants and who participated in the 2013 to 2015 Korea National Health and Nutrition Examination Survey were reviewed. Periodontal status was assessed using Community Periodontal Index (CPI) scores, and periodontitis was defined as a CPI greater than or equal to 3. The complex sampling design of the survey was utilized to obtain the variance and individual weight of each analyzed factor. A high CPI was the outcome variable, and the main explanatory variables were oral hygiene behaviors, such as TB, dental floss (DF), interproximal brushing, and mouth rinsing. Results: Almost all individuals with a lower CPI brushed their teeth twice or more per day, in contrast to those with a higher CPI, and were likely to use DF. The adjusted odds ratio of not using DF for a higher CPI was 1.83 (95% confidence interval, 1.35-2.49). Conclusions: TB was implemented more than twice a day by patients with good oral health, and the combination of TB and DF significantly reduced the prevalence of a higher CPI. Self-performed oral hygiene practices combining TB and DF were significantly related to a low prevalence of periodontitis in implant patients.

• Journal of dental hygiene science
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• v.14 no.2
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• pp.123-131
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• 2014

Environmental tobacco smoke (ETS) or second hand smoke or passive smoking has become a well-known risk factor for various health hazards in nonsmoking adults as well as active smokers. In Korea, there have been few studies concerning about the impact of ETS on periodontal health. Therefore, the aim of this study was to investigate the association between exposure to passive smoking and prevalence of periodontitis using Korea National Health and Nutrition Examination Survey (KNHANES) in 2008 and 2009. The Fourth KNHANES data was employed from 2008~2009. The final sample size was 4,669 adults aged over 18 years, who were never-smoker and had the information about ETS both at home and in the workplace, urine analysis and periodontal examination by Community Periodontal Index (CPI). Periodontitis was defined as CPI codes ${\geq}3$. Data were analyzed using PASW Statistics 18.0. The sociodemographic and behavioral factors were adjusted as confounders. Overall, 17.1% (male 16.4%, female 83.6%) of the participants were exposed to ETS. The mean concentration of cotinine in those exposed ETS was significantly higher than that in unexposed people ($46.92{\mu}g/ml$ versus $19.34{\mu}g/ml$, p<0.001). Participants exposed to ETS were more likely to have periodontitis than those unexposed after adjusting for potential confounding variables. ETS is associated with the prevalence of periodontitis in Korean adults. This may suggest that patients with periodontitis or periodontal surgery should be protected from smokers or smoking places.

• KDI Journal of Economic Policy
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• v.45 no.4
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• pp.57-77
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• 2023

The Chonsei component holds the highest level of weight (5.4%) in the composition of the Korean consumer price index (CPI). The variations in Chonsei prices are directly reflected in the CPI as a representation of cost swings. The Chonsei refers to a deposit that accumulates the costs related to housing services and is mostly affected by variations in rental rates. Nevertheless, it is important to note that Chonsei prices are also susceptible to fluctuations in interest rates, regardless of the rent prices. Therefore, if Chonsei were directly and one-to-one indexed to the CPI, they could include changes other than residential service prices. After analyzing the time series data of the Chonsei index and rent index inside the CPI, it becomes apparent that the Chonsei index displays an average annual growth rate of 2.3%, whilst the rent index reveals a growth rate of 0.9%. The observed disparity in growth rates indicates a divergence in trends between the two indices. It is posited that the Chonsei index, when capitalized, has had a more rapid increase compared to the rental index, owing to the gradual drop in interest rates. To effectively reflect fluctuations in the housing service costs, proxies for the Chonsei index were utilized in the construction of a consumer price index. The findings of our study suggest that, overall, the newly developed CPI demonstrates a comparatively lower rate of inflation when compared to the official CPI. Furthermore, the inclusion of imputed rents for owner-occupied housing in CPI amplifies this effect.

• Journal of the Korean Society of Clothing and Textiles
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• v.24 no.5
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• pp.748-759
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• 2000

The purposes of this study were 1) to classify wearing situation of male adolescents and 2) to classify self-image and CPI(Clothing Preference Image) of male adolescents and 3) to segment consumer group by self-image and to find the differences in self-image and CPI by situation among groups. For the data collection a questionnaire was distributed to male adolescents who were residents in Seoul and Taejeon. The statistics used for the data analysis were factor analysis, multiple dimensional scale, mean, percentage, peason-correlation, cluster analysis, one-way ANOVA, Duncan-test by the SPSSWIN program. The results of this study are as follows: 1) The self-image of male adolecents is categorized by seven factors; sophisticate and fashion conscious, active, practical and realistic, flank and pure, young-looking, feminine, and slender. Based on seven factors, the consumer group is categorized to five groups; practical and realistic Group1, young-looking and feminine Group2, characterless Group3, active Group4, sophisticate and flank Group5. 2) Wearing situations are divided into three categories; in downtown, in urban, at festival. In downtown, CPI are divided into six elements; ornamental, simplex, sexy, feminine, neat, young, and sophisticate. In urban, CPI are divided into five elements; ornamental, simple, sexy, feminine, young-looking, and sophisticate. At festival, CPI are divided into four elements; unique, simple, feminine, and formal. To conclude, the male adolescent consumers are categorized by self-image, and the different CPIs are sought by different wearing situations.