• Title/Summary/Keyword: CP2

Search Result 2,657, Processing Time 0.039 seconds

Influence of Dietary Conjugated Linoleic Acid (CLA) and Carotenoids on Growth, Fatty Acid Composition, and 3T3-L1 Cells in Black Seabream (Acanthopagrus schlegeli) (CLA 첨가사료가 감성돔(Acanthopagrus schlegeli) 성장과 지방산 조성 및 내장 추출지방이 지방세포 3T3-L1에 미치는 영향)

  • Guo, Rui;Rohmah, Zuliyati;Choi, Kwang-Soo;Park, Si-Hyang;Ha, Yeong-Lae;Kang, Seok-Joong;Choi, Byeong-Dae
    • Journal of Life Science
    • /
    • v.25 no.5
    • /
    • pp.548-556
    • /
    • 2015
  • Three groups of black seabream (Acanthopagrus schlegeli) were fed with treatment diets containing certain concentrations of conjugated linoleic acid (CLA) and carotenoids. The control group feed contained 0% CLA and 0% carotenoids, the CP10 group feed contained 1% CLA and 0.1% carotenoids, and the CP25 group feed contained 2.5% CLA and 0.1% carotenoids. The CP10 and CP25 groups demonstrated the enhanced growth and increased feed conversion efficiency of black seabream. The specific growth rates (SGRs) were 0.74, 0.81, and 0.97, while the feed conversion ratios (FCRs) were 2.65, 2.46, and 2.04 for the control, CP10, and CP25 groups, respectively. The total contents of high unsaturated fatty acid (HUFA) for the control, CP10, and CP25 groups were 41.0%, 41.7%, and 43.5%, respectively. CLA was deposited to the extent of 2.8% and 5.6% in the muscle, and 4.0% and 8.3% in the viscera of the CP10 and CP25 groups, respectively. Meanwhile, treatment with the viscera lipid extract (VLE) from CP25 fish evidently lowered 3T3-L1 adipocytes viability. The lipid extract from the muscle and viscera of black seabream contained ample amounts of beneficial substances, such as CLA, carotenoids, EPA, and DHA. CLA, which enriched black seabream muscle, could be categorized as a functional food and serve as a well-being food. Meanwhile, the fish oil from its viscera could serve as a high function supplement.

Free Amino Acid and Polysaccharide Content of Submerged Mycelial Culture of Fomitopsis pinicola (귤피 및 녹차추출물에서 배양한 소나무잔나비버섯(Fomitopsis pinicola) 균사체의 유리아미노산 및 다당류 함량)

  • Jang Kyung-Ho;Shin Kyung-Ok;Kim Soon-Dong
    • Food Science and Preservation
    • /
    • v.12 no.4
    • /
    • pp.379-386
    • /
    • 2005
  • Free amino acids and polysaccharide content of submerged mycelial culture of Fomitopsis pinicola using $2\%$ citrus peel water extracts(CP), $2\%$ green tea water extracts(GT) and yeast-malt broth(YM) were investigated. Cultivations were conducted at $30^{\circ}C$ and 150 rpm for 10 days. Yields of the mycelium as fresh weight basis in GT-, CP- and YM-broth were $42.3\%,\;34.2\%\;and\;9.89\%$, and their turbidity(OD at 660 nm) of the broth without mycelium were 0.14, 0.16 and 0.22, respectively. Total free amino acid content in the mycelium were 928.19 $mg\%$ in YM, 1060.53 $mg\%$ in CP, 764.83 $mg\%$ in GT, and the major free amino acid was lysine in YM, glutamic acid in CP and GT. Total free exo-amino acid contents were 659.75 $mg\%$ in YM, 954.55 $mg\%$ in CP, 838.69 $mg\%$ in GT, and the major in the all broths was glutamic acid Total amino acid derivatives content of the mycelium were in order of CP>GT>YM, and the major was cystathionine in YM, hydroxy proline in CP and GT. The major among exo-amino derivatives was hydroxy proline in the all broth. Exo-AIS content was in order of CP>GT>YM. Acid soluble polysaccharide content of the mycelium was GT($0.69\%$)>YM($0.39\%$)>CP($0.18\%$). The exo-polysaccharide content was in order of GT($0.87\%$)>CP($0.69\%$)>YM($0.09\%$). Alkali soluble polysaccharide content of the mycelium was in order of CP($5.21\%$)>GT($5.18\%$)>YM($4.56\%$), and exo-polysaccharide was in order of GT($6.79\%$)>YM($3.57\%$)>CP($3.01\%$). The alkali soluble polysaccharide eluted from mycelium cultivated in CP broth was supposed to polysaccharide(about 500,000 daltons) composed of hexose and uronic acid bounded with protein(below 10,000 daltons).

Production of Polyethylene Wax via Metallocene Catalysts [(TMDS)$Cp_2$]$ZrCl_2$ and [$(n-Bu)_2Cp_2$]$ZrCl_2$ in the Presence of Hydrogen Gas as a Chain Transfer Reagent (메탈로센 화합물인 [(TMDS)$Cp_2$]$ZrCl_2$ 촉매와 [$(n-Bu)_2Cp_2$]$ZrCl_2$ 촉매를 이용한 고품질의 폴리에틸렌 왁스 제조)

  • Kim, Ji-Yoon;Yoon, Seok-Young;Yang, Young-Do;Noh, Seok-Kyun
    • Polymer(Korea)
    • /
    • v.32 no.6
    • /
    • pp.566-572
    • /
    • 2008
  • Polyethylene has been prepared via metallocene catalysts [(TMDS)$Cp_2$]$ZrCl_2$, 1, and [$(n-Bu)_2Cp_2$]$ZrCl_2$, 2, in the presence of hydrogen as a chain transfer reagent. Increase of hydrogen flow to the polymerization reactor resulted in the drop of catalytic activity, reduction of molecular weight of polyethylene, getting narrow of molecular weight distribution of polyethylene, and melting point of the polyethylene wax. It should be noticed that it was possible to control molecular weight down to 1500 and melting temperature to 60 $^\circ$C of polyethylene wax using the catalyst 1 that has been developed by authors as well as the catalyst 2 from Exxon.

Effect of Cheongpyesagan-tang on LPS Induced Inflammation in RAW 264.7 Cells (LPS로 유도된 RAW 264.7 cell의 염증반응에서 청폐사간탕(淸肺瀉肝湯)의 항염증 효과)

  • Kim, Tae Yeon;Leem, Kang Hyun
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.33 no.1
    • /
    • pp.31-38
    • /
    • 2019
  • Cheongpyesagan-tang (CP) is one of the traditional medicinal prescription to treat Taeumin (太陰人)'s disease. It has been commonly used for the treatment of stroke, arthritis, diabetes and obesity. In this study, we investigated an anti-inflammatory potential of CP water extract. We examined the effects of CP on the lipopolysarccharide (LPS)-induced production of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$). We also examined the levels of protein or mRNA of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and proinflammatory cytokines in RAW 264.7 cells. CP inhibited NO and $PGE_2$ production in a dose dependent manner and decreased the protein and mRNA expression of iNOS and COX-2. Also, CP decreased the mRNA expression of interleukin-6 (IL-6), interleukin-$1{\beta}$ (IL-$1{\beta}$), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$). These results suggest that CP has potential as anti-inflammatory therapeutic medicine.

Prediction of apparent total tract digestion of crude protein in adult dogs

  • Kangmin Seo;Hyun-Woo Cho;Min Young Lee;Chan Ho Kim;Ki Hyun Kim;Ju Lan Chun
    • Journal of Animal Science and Technology
    • /
    • v.66 no.2
    • /
    • pp.374-386
    • /
    • 2024
  • To predict the apparent total tract digestibility (ATTD) of crude protein (CP) in dogs we developed an in vitro system using an in vitro digestion method and a statistical analysis. The experimental diets used chicken meat powder as the protein source, with CP levels of 20% (22.01%, analyzed CP value as dry-based), 30% (31.35%, analyzed CP value as dry-based), and 40% (41.34%, analyzed CP value as dry-based). To simulate in vivo digestive processes a static in vitro digestion was performed in two steps; stomach and small intestine. To analyze ATTD the total fecal samples were collected in eight neutered beagle dogs during the experimental period. CP digestibility was calculated by measuring CP levels in dog food, in vitro undigested fraction, and dog feces. In result, CP digestibility at both in vivo and in vitro was increased with increasing dietary CP levels. To estimate in vivo digestibility the co-relation of in vivo ATTD and in vitro digestibility was investigated statistically and a regression equation was developed to predict the CP ATTD (% = 2.5405 × in vitro CP digestibility (%) + + 151.8). The regression equation was evaluated its feasibility by using a commercial diet. The predicted CP digestibility which was calculated by the regression equation showed high index of similarity (100.16%) with that of in vivo in dogs. With that, it would be a feasible non-animal method to predict in vivo CP digestibility by using in vitro digestion method and the proposed linear regression equation in adult dogs.

Rhodium(III)-mediated cycloaddition reactions of alkynes

  • 한원석;이순원
    • Proceedings of the Korea Crystallographic Association Conference
    • /
    • 2002.11a
    • /
    • pp.30-30
    • /
    • 2002
  • Heating [Cp*Rh(η²-NO₃)(OTf) (1) and PhC≡CPh in EtOH for 3 h gave a η⁴-cyclobutadienerhodium complex, [Cp*Rh(η⁴-C₄Ph₄)] (2). Complex 1 reacted with HC=CPh in acetone at room temperature for 3 h to give a (η⁴-cyclobutadiene)-rhodium complex, [Cp*Rh(η⁴-C₄HPhC=CPh)] (3). Whereas, the reactions of 1 with HC=CCH₂Cl in acetone at room temperature for 3 h gave the triply halide-bridged dinuclear rhodium complex, [Cp*Rh(μ₂-Cl)₃RhCp*](OTf) (4). Complexes 2-4 have been structurally characterized by X-ray diffraction.

  • PDF

An In Vitro Method to Estimate Apparent Ileal Crude Protein Digestibility in Feed Ingredients Fed to Broiler Chickens (육계에서의 외관상 회장 조단백질 소화율 추정을 위한 In Vitro 실험방법)

  • Su Hyun, An;Changsu, Kong
    • Korean Journal of Poultry Science
    • /
    • v.49 no.4
    • /
    • pp.273-279
    • /
    • 2022
  • The objective of this study was to evaluate an in vitro procedure to estimate the crude protein (CP) digestibility of feed ingredients and mixed diets in broiler chickens. The apparent ileal digestibility (AID) of the CP was measured using 23-d-old male broilers. Three experimental diets, containing three feed ingredients, namely soybean meal (SBM), canola meal (CM), and corn distiller's dried grains with solubles (DDGS), were used as the sole source of CP. A 2-step in vitro procedure was used to estimate in vivo CP digestibility; all the experiments were performed in triplicate. In step 1, the feed ingredient and mixed diet samples were incubated for 4 h at 40 °C with a pH 2.0 pepsin solution, and in step 2, the flasks were incubated for 12 h at 40 °C with a pH 6.8 pancreatin solution. Following incubation, all the samples were filtered; the undigested residues were collected and pooled together to analyze the undigested CP concentration. The in vitro CP digestibility of mixed diets and feed ingredients were 93.2% and 93.0% for SBM, 86.8% and 86.7% for CM, and 83.8% and 79.1% for DDGS, respectively. The coefficients of determination (R2) between the CP digestibility values for the feed ingredients and the in vitro CP digestibility values for the feed ingredients or respective mixed diets were 0.87 or 0.67. The results of the study demonstrated that the in vitro CP digestibility values obtained from the respective mixed diets were better estimates than the values obtained from the individual feed ingredients to predict the AID of CP in feed ingredients fed to broiler chickens.

Performance, Meat Quality and Blood Composition of Cross Bred Chicks Fed Various Organic Dietary CP and ME (ME와 CP가 다른 유기사료 급여가 토종닭의 생산능력 계육품질 및 혈액성상에 미치는 영향)

  • Park, Jae-Hong;Jeong, Yong-Dae;Yoon, Myung-Ja;Ryu, Kyeong-Seon
    • Korean Journal of Organic Agriculture
    • /
    • v.19 no.2
    • /
    • pp.199-214
    • /
    • 2011
  • This study was conducted to investigate the effect of organic dietary metabolizable energy (ME) and crude protein (CP) on productivity, meat quality and blood composition of crossbred chicks (hanhyub 3 ho) for different growring periods. Experiments were factorially designed with ME 3,000, 3,100kcal/kg and CP 21, 22, 23% for starter (0-4wks); ME 3,100, 3,150, 3,200kcal/kg and CP 18, 19, 20% for grower (5-8wks); ME 3,100, 3,150, 3,200kcal/kg and CP 15, 16, 17% for finisher (9-10wks). The total number of chicks and replicate of each treatment for starter, grower, finisher were 720, 4; 702, 6; 468, 4, respectively. The diets was mixed with more 90% organic feed ingredients. The productivity were not influence by dietary ME for starting period. Weight gain and feed intake were significantly increased in CP 23% treatment than CP 21, 22% treatment (P<0.05). FCR was improved as dietary CP increased (P<0.05). Weight gain was tended to be increased by decreaing ME content and increasing CP contents for growing period. Feed intake showed no difference among the ME and CP treatment groups. FCR improved significantly in CP 19, 20% compared with CP 18% (P<0.05). Weight gain was not significantly different between ME and CP treatments for finishing period. Feed intake was lower in ME 3,150, 3,200kcal/kg than the ME 3,100 kcal/kg treatment (P<0.05). FCR was higher in ME 3,100kcal/kg than ME 3,150, 3,200kcal/kg treatment (P<0.05). Interaction on productivity was not exited between ME and CP treatment groups for different feeding periods. There was no difference in the total protein, albumin, glucose and triglyceride amount in blood depending on dietary ME and CP contents. Total cholesterol was greatly decreased in ME 3,100kcal/kg than the ME 3,200kcal/kg (P<0.05), but not different between CP treatments. Cooking loss of breast meat was lower in ME 3,150kcal/kg than the ME 3,200kcal/kg (P<0.05). Moisture, shear force and pH were not statistically different among treatments. Protein solubility was increased by increasing ME and CP in diets. The collagen was tended to increase as dietary ME increased. Redness was remarkably higher in ME 3,150kcal/kg than the ME 3,100kcal/kg (P<0.05). Yellowness of meat fed ME 3,150kcal/kg showed significantly lower than other treatments (P<0.05). Therefore, the optium ME and CP to improve the productivity for each period were 3,000kcal/kg, 23%; 3,100kcal/kg, 19%; 3,150kcal/kg, CP 16%, repectively.

A Charge Pump Circuit in a Phase Locked Loop for a CMOS X-Ray Detector (CMOS X-Ray 검출기를 위한 위상 고정 루프의 전하 펌프 회로)

  • Hwang, Jun-Sub;Lee, Yong-Man;Cheon, Ji-Min
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
    • /
    • v.13 no.5
    • /
    • pp.359-369
    • /
    • 2020
  • In this paper, we proposed a charge pump (CP) circuit that has a wide operating range while reducing the current mismatch for the PLL that generates the main clock of the CMOS X-Ray detector. The operating range and current mismatch of the CP circuit are determined by the characteristics of the current source circuit for the CP circuit. The proposed CP circuit is implemented with a wide operating current mirror bias circuit to secure a wide operating range and a cascode structure with a large output resistance to reduce current mismatch. The proposed wide operating range cascode CP circuit was fabricated as a chip using a 350nm CMOS process, and current matching characteristics were measured using a source measurement unit. At this time, the power supply voltage was 3.3 V and the CP circuit current ICP = 100 ㎂. The operating range of the proposed CP circuit is △VO_Swing=2.7V, and the maximum current mismatch is 5.15 % and the maximum current deviation is 2.64 %. The proposed CP circuit has low current mismatch characteristics and can cope with a wide frequency range, so it can be applied to systems requiring various clock speed.

Application of cold plasma treatment as a method to improve the physical properties of defatted mustard meal-based edible films (탈지 겨자씨 소재 가식성 필름의 물리적 특성 향상을 위한 콜드 플라즈마의 적용)

  • Jeong, Ha Eun;Oh, Yoon Ah;Min, Sea Cheol
    • Korean Journal of Food Science and Technology
    • /
    • v.53 no.5
    • /
    • pp.634-639
    • /
    • 2021
  • This study investigated the effects of cold plasma (CP) treatment on the properties of biopolymer films prepared with defatted mustard meal (DMM films). CP treatments using N2, O2, He, Ar, and dry air did not affect the tensile properties, water vapor permeability, color, and morphology of DMM films, whereas the treatments using He and Ar improved their printability. The tensile strength (TS) of O2- or air-CP-treated DMM films and the elastic modulus (EM) of O2-, He-, Ar-, or air-CP-treated films were lower than those of the untreated films. An increase in the power of Ar-CP treatment resulted in an increase in EM. The optimum treatment power and time for minimizing yellowness changes by Ar-CP treatment were 420 W and 40 min, respectively. The results demonstrated the potential application of CP treatment to improve the film properties of DMM films and possibly other agricultural by-product-based biopolymer films, making the films more applicable to food packaging.