• Asian-Australasian Journal of Animal Sciences
• /
• v.25 no.4
• /
• pp.515-523
• /
• 2012

The objective of this study was to investigate the variability in broiler performance, apparent metabolisable energy (AME) and ileal digestible energy (IDE) between five different maize samples fed with and without xylanase at 16,000 U/kg. Various in vitro characterisations were conducted to determine if any could predict performance or AME. Samples of the maize were harvested in five diverse regions and fed individually in a mash diet as follows (g/kg): test maize 608.3; soya bean meal (SBM) 324.1; poultry fat 25.2; salt 4.6; met 2.6; lys 1.6; thr 0.5; limestone 9.7, dical 18.4; vit/min 5.0; CP 210 and ME (kcal/kg) 3,085. The diets were fed to 720 broilers with 6 replicates, each containing 12 birds per treatment, from 0 to 18 d of age. Maize samples were analysed for starch, protein, crude fibre, fat, protein solubility index (PSI) and vitreousness using near infra red reflectance spectroscopy (NIR). They were also assayed using an in vitro starch digestibility method. The results showed that there was no effect of harvest region on the feed intake (FI), body weight gain (BWG) or feed conversion ratio (FCR) of the broilers over the 18 d period (p = 0.959, 0.926, 0.819 respectively). There was an improvement in all parameters with the addition of xylanase (FI p = 0.011; BWG and FCR p<0.001). There was a significant positive effect of xylanase on IDE, AME, IDE Intake (IDEI) and AME intake (AMEI) (p<0.0001 in all cases). Although there was no significant effect of maize source, there was a strong trend towards variability in IDE (p = 0.066) and AME (p = 0.058). There were no significant correlations (p<0.05) between any proximate or physiochemical values and any performance or AME values. This may suggest that none of those selected were suitable predictors for performance or AME. The broilers performed well according to the breed guidelines, with slightly increased FI, increased BWG and similar FCR prior to the addition of xylanase. When FCR and BWG were analysed with FI as a covariate, xylanase addition remained significant suggesting that the improvement in BWG and FCR was driven by an increase in digestibility and nutrient availability.

• Asian-Australasian Journal of Animal Sciences
• /
• v.25 no.11
• /
• pp.1568-1574
• /
• 2012

Three Holstein steers in the growing phase, each with a ruminal cannula, were used to test the hypothesis that the synchronization of the hourly rate of carbohydrate and nitrogen (N) released in the rumen would increase the amount of retained nitrogen for growth and thus improve the efficiency of microbial protein synthesis (EMPS). In Experiment 1, in situ degradability coefficients of carbohydrate and N in feeds including Korean rice wine residue (RWR) were determined. In Experiment 2, three total mixed ration (TMR) diets having different rates of carbohydrate and N release in the rumen were formulated using the in situ degradability of the feeds. All diets were made to contain similar contents of crude protein (CP) and neutral detergent fiber (NDF) but varied in their hourly pattern of nutrient release. The synchrony index of the three TMRs was 0.51 (LS), 0.77 (MS) and 0.95 (HS), respectively. The diets were fed at a restricted level (2% of the animal's body weight) in a $3{\times}3$ Latin-square design. Synchronizing the hourly supply of energy and N in the rumen did not significantly alter the digestibility of dry matter, organic matter, crude protein, NDF or acid detergent fiber (ADF) (p>0.05). The ruminal $NH_3$-N content of the LS group at three hours after feeding was significantly higher (p<0.05) than that of the other groups; however, the mean values of ruminal $NH_3$-N, pH and VFA concentration among the three groups were not significantly different (p>0.05). In addition, the purine derivative (PD) excretion in urine and microbial-N production (MN) among the three groups were not significantly different (p>0.05). In conclusion, synchronizing dietary energy and N supply to the rumen did not have a major effect on nutrient digestion or microbial protein synthesis (MPS) in Holstein steers.

• Asian-Australasian Journal of Animal Sciences
• /
• v.25 no.3
• /
• pp.335-340
• /
• 2012

The data used came from two trials undertaken under the same climatic conditions (spring-summer). In both trials pluriparious buffaloes were utilized similar in weight, body condition score, and milk production from the previous year. From the first trial the data used was from the sub-period 23-88 DIM provided by seven animals fed ad libitum with diet A (6.69 MJ/kg DM; 158.30 g/kg of crude protein) with a forage/concentrate ratio of 48/52. From the second trial the data used was from the sub-period 33-90 DIM provided by seven animals fed ad libitum with diet B (6.63 MJ/kg DM; 179.50 g/kg of crude protein) and by seven animals fed ad libitum with diet C (5.99 MJ/kg DM; 155.40 g/kg of crude protein), each of the diets had the same forage/concentrate ratio (53/47). A significant difference was found in milk production between group B and C (13.08 vs. 11.56 kg/d, p<0.05), an intermediate production (12.10 kg/d) was noted in group A. A significant difference was found between fat (76.58 vs. 69.24 g/kg, p<0.05), protein (46.14 vs. 43.16 g/kg, p<0.05) and casein (39.94 vs. 34.98 g/kg, p<0.05) of the milk of group B with respect to group A. The milk of group C gave fat values (71.80 g/kg), protein (45.52 g/kg) and casein (39.06 g/kg) statistically equal to those of group B. The milk of groups B and C, in respect to the milk of group A, gave values of $K_{20}$ (1.77, 1.82 vs. 3.68 min, p<0.05), statistically lower and values of $A_{30}$ (48.28, 47.27 vs. 40.64 mm, p<0.05) statistically higher. Two simple linear regressions were calculated where the independent variable (x) was the daily standardized milk production, the dependent variable (y) or the daily intake of net energy or crude protein. Equation 1) NE (MJ/d) = 74.4049+2.8308${\times}$kg of normalized milk; equation 2) CP (kg/d) = 1.4507+0.1085${\times}$kg of normalized milk, both the equations were significant (p<0.05) with determination coefficients of 0.58 and 0.50 respectively. For a production of normalized milk that varies from 9 to 13 kg, the respective energy-protein concentrations fluctuate from 6.09 to 6.78 MJ/kg DM and from 148.00 to 174.46 g/kg DM.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.8
• /
• pp.3495-3501
• /
• 2014

Melanoma-associated antigen (MAGE) family genes have been considered as potentially promising targets for anticancer immunotherapy. MAGED4 was originally identified as a glioma-specific antigen. Current knowledge about MAGED4 expression in glioma is only based on mRNA analysis and MAGED4 protein expression has not been elucidated. In the present study, we investigated this point and found that MAGED4 mRNA and protein were absent or very lowly expressed in various normal tissues and glioma cell line SHG44, but overexpressed in glioma cell lines A172,U251,U87-MG as well as glioma tissues, with significant heterogeneity. Furthermore, MAGED4 protein expression was positively correlated with the glioma type and grade. We also found that the expression of MAGED4 inversely correlated with the overall methylation status of the MAGED4 promoter CpG island. Furthermore, when SHG44 and A172 with higher methylation were treated with the DNA demethylating agent 5-aza-2'-deoxycytidine (5-AZA-CdR) reactivation of MAGED4 mRNA was mediated by significant demethylation in SHG44 instead of A172. However, 5-AZA-CdR treatment had no effect on MAGED4 protein in both SHG44 and A172 cells. In conclusion, MAGED4 is frequently and highly expressed in glioma and is partly regulated by DNA methylation. The results suggest that MAGED4 might be a promising target for glioma immunotherapy combined with 5-AZA-CdR to enhance its expression and eliminate intratumor heterogeneity.

• Asian-Australasian Journal of Animal Sciences
• /
• v.19 no.6
• /
• pp.892-897
• /
• 2006

An experiment was designed to study the nutritional value of Chinese brown rice and maize for growing pigs. Six male grower pigs (Duroc${\times}$Landrace${\times}$Large White, $24.3{\pm}1.26$ kg average initial BW) were surgically fitted with a simple T-cannula at the terminal ileum and allotted within a $2{\times}2$ Latin square design. The pigs were fed either a maize or brown rice diet in a direct method to determine their digestibility. The brown rice used in this experiment was husked from one kind of early, long grain, and non-glutinous rice (ELGNR, indica rice: non-waxy rice, containing amylopectin and amylose) in southern China. Chromic oxide was used as a marker. The diets were supplied at about 4.0% of body weight in dry matter/d. Total faeces and urine were collected on days 4 and 5; digesta was collected on days 6-8 in each period. The average body weight was 24.3 kg at the start of the experiment and 27.6 kg at the end. The results showed that the apparent ileal digestibilities of most amino acids of brown rice were significantly higher than those in maize (p<0.01), as were the apparent ileal digestibilities of crude protein (CP), digestible energy (DE), organic matter (OM) and dry matter (DM) (p<0.05). However, the apparent ileal starch digestibilities of the two treatments were similar (p>0.05). The values of the apparent faecal digestibilities derived from the two methods, marker and total faecal-collection methods, were very similar and also correlated with each other. The difference in absolute value of the apparent faecal digestibilities between brown rice and maize was smaller compared to that of the apparent ileal digestibilities. The net protein utilization was higher (p = 0.07) and the DE metabolizable rate was significantly higher (p<0.01) for brown rice than for maize. The metabolizable energy (ME) of brown rice is similar to that of maize, while the DE of brown rice was relatively lower. It can be concluded that Chinese brown rice are better than maize not only in apparent ileal digestibilities, but also in metabolizable rate of amino acids and gross energy under the present study conditions.

• Asian-Australasian Journal of Animal Sciences
• /
• v.18 no.4
• /
• pp.475-478
• /
• 2005

This study was conducted to evaluate feeding value of berseem and lucerne silage as a replacement for conventional fodder (berseem fodder) in lactating Nili buffaloes. Fifteen early lactating multi-parous Nili buffaloes, five buffaloes in each group were allotted three experimental diets. Berseem and lucerne fodders were ensiled at 30% DM (wheat straw was used to adjust the DM of fodders) with molasses (at the rate of 2% of fodder DM) in two bunker silos for 30 days. The diets contained 75% DM from berseem fodder (BF), 75% DM from berseem silage (BS) and 75% DM from lucerne silage (LS). Each diet contained 25% concentrate DM. Diets were mixed daily and fed twice a day at ad libitum intakes. Dry matter intake (DMI) was significantly higher (13.8 kg/d) in buffaloes fed BF diet than those fed LS (12.5 kg/d) and BS (11.9 kg/day) diets. The differences in digestible DMI and DMI as percent body weight were significant between fodder and silage based diets but non-significant when BS and LS were compared. Lower DMI with silage-based diets was probably because of low silage pH. Intake of NDF (NDFI) was higher (5.68 kg/d) in buffaloes fed BF diet followed by those fed LS (5.50 kg/d) and BS (5.00 kg/d) diets. The difference was significant (p<0.05) across fodder and silage based diets but NDFI was non-significant across both silage-based diets. The apparent DM digestibility was significantly different (p<0.05) between fodder and silage-based diets but was non-significant between LS and BS diets. Four percent fat corrected milk yield was significantly different (p<0.05) between fodder and silage-based diets but was non-significant between LS and BS diets. Higher milk yield with fodder based diet was because of more digestible nutrient intake (Table 3) compared with silage based diets. Milk CP, TP and NPN and SNF did not show any treatment effects. The present results indicated that the berseem and lucerne fodder ensiled at 30% DM level with 2% molasses could safely replace (75% DM) the conventional leguminous fodder in the diets of lactating Nili buffaloes.

• Asian-Australasian Journal of Animal Sciences
• /
• v.22 no.7
• /
• pp.1016-1025
• /
• 2009

Two experiments were conducted to determine energy and nutrient digestibility in four sources of distillers dried grains with solubles (DDGS) produced from corn and fed to growing pigs. The objective was to test the hypothesis that energy and nutrient digestibility in DDGS does not vary if samples are procured from ethanol plants that use similar production technologies and use corn that is grown within a narrow geographical area. The four sources of DDGS that were used were procured from ethanol plants that were less than 10 yr old and located within 250 km of each other. In Exp. 1, five growing barrows (initial BW = 71.4 kg) that were fitted with a T-canula in the distal ileum were allotted to a 5${\times}$5 Latin square design and used to measure apparent (AID) and standardized (SID) ileal digestibility of AA in the four sources of DDGS. Results of this experiment showed that the SID of CP and all AA except Cys and Pro were greater (p<0.05) in two of the DDGS sources than in the other two sources. Exp. 2 was conducted to measure the concentration of DE and ME and the apparent total tract digestibility (ATTD) of energy, N, P, ether extract, NDF, and ADF in corn and in the same four sources of DDGS as used in Exp. 1. Five pigs (initial BW = 29.7 kg) that were placed in metabolism cages and allotted to a 5${\times}$5 Latin square design were used. Results of Exp. 2 showed that the average DE and ME in DDGS were 4,072 and 3,750 kcal/kg DM, respectively, which was less (p<0.01) than the DE and ME in corn (4,181 and 4,103 kcal/kg DM, respectively). The average ATTD for P in DDGS was 56.1%, which was greater (p<0.01) than the ATTD for P in corn (31.9%). The ATTD for ADF in DDGS was also greater (p<0.05) than in corn, but the ATTD for ether extract and NDF were greater (p<0.05) in corn than in DDGS. It is concluded that energy and nutrient digestibility vary among sources of DDGS even when the DDGS is procured from ethanol plants that use corn grown within a narrow geographical region. Thus, factors other than corn growing region are responsible for the variability of energy and nutrient digestibility in DDGS.

• Asian-Australasian Journal of Animal Sciences
• /
• v.19 no.12
• /
• pp.1755-1760
• /
• 2006

Studies were carried out at the National Institute of Animal Nutrition and Physiology, India to determine the effect of feeding chopped paddy straw (Oryza sativa) on the energy expenditure in crossbred cattle. Four crossbred cattle male, aged 5-6 years and weighing about 450 kg were used for this study. Three experimental trials, one each for the feeding of un-chopped paddy straw offered ad libitum (UCA), chopped paddy straw fed at restricted level (CR) and chopped paddy straw offered ad libitum (CA) were conducted. The quantity of un-chopped paddy straw consumed during UCA was assumed as the voluntary intake by the cattle and the same quantity was offered after chopping during CR. Each trial comprised of 21 d preliminary feeding period and 5 d of observation recording period. Expired gas was collected in Douglas bags using a face-mask and three-way valve at 6 hourly intervals i.e., at 09.30, 15.30, 21.30, and 03.30 h throughout the observation period. Expired gas and ambient air inspired by the animals were analyzed for the oxygen content through paramagnetic oxygen analyzer. Energy expenditure (EE) by the animals was calculated by determining the volume of oxygen consumed per minute (STP) and multiplying by 4.825. Paddy straw used in all the three trials contained (g/kg DM) 90.0 CP, 786 OM, 700 NDF, 489 ADF, 357 Cellulose and 60.0 ADL. Metabolizable energy (ME) was 6.9 MJ/kg DM. Dry matter intake (DMI) both in UCA and CR was about 6.8 kg, except that it was chopped in CR. Chopping has resulted in 32% improvement (9 kg) in DMI of CA as compared to that of UCA. Although ME intake was similar in UCA and CR (47.2 MJ/day), energy expenditure (EE) was higher in UCA (23.3 MJ) when compared to that of CR (19.5 MJ). The ME intake (63.3 MJ) as well as EE (27.1 MJ) was highest in CA. Energy expenditure when expressed as MJ/kg DMI was 3.48, 2.90 and 3.12; whereas as per cent of ME intake it was 50, 41 and 44 in UCA, CR and CA respectively. Our study has unequivocally confirmed that chopping of poor quality roughages like paddy straw has definite advantages not only in terms of improving the intake by decreasing the time taken for ingestion but also in reducing the energy cost of eating.

• Korean Journal of Organic Agriculture
• /
• v.17 no.3
• /
• pp.347-356
• /
• 2009

In attempt to select the most appropriate forage legume species, this study was conducted on the 10 forage legume crops suitable for the central region of Korea and investigated their productivity, feed values and stock carrying capacity. In the results, red clover showed fresh matter yield 38.6 ton/ha, dry matter yield 7.8 ton/ha, crude protein yield 1.0 ton/ha, total digestible nutrients(TDN) yield 4.6 ton/ha, its fresh matter yield, dry matter yield and nutrient yield were significantly(p<0.05) higher than other species. The value of neutral detergent fiber(NDF) and acid detergent fiber(ADF) were getting higher by the late harvest time then galega and cicer milkvetch were the highest and yellow suckling clover was the lowest value. Relative feed value(RFV) showed good feed values above 100 except cicer milkvetch and galega. In terms of feed grading, yellow suckling clover, yellow blossom sweet clover, crimson clover, hairy vetch were classified into 1st grade, above 151%. The average stock carrying capacity was yet high Kcp(2.18 head/ha/yr), KTDN(1.25 head/ha/yr) and K(0.04 head/ha/yr). Especially, red clover had the highest stock carrying capacity, Kcp 6.84 head/ha/yr, KTDN 3.64 head/ha/yr and K 0.05 head/ha/yr. According to results, red clover is considered to be the most appropriate crops for the central region, in terms of quality, feed value and stock carrying capacity.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.32 no.2
• /
• pp.131-138
• /
• 2012

This study was conducted to evaluate the characteristics and forage production of bermudagrass (Cynodon dactylon) and bahiagrass (Paspalum notatum) in Jeju. Bermudagrass cultivars evaluated were Common and Ecotype. Bahiagrass cultivars evaluated were Tifton 9 and Argentine. Two warm season grasses were established at the Subtropical Animal Experiment Station in spring 2007. Emergence of bremudagrass and bahiagrass was observed approximately 16 days and 28 days after seeding, respectively. The heading dates of bermudagrass and bahiagrass were on 26 May and in mid-July, respectively. Bermudagrass cultivars had higher dry matter (DM) than bahiagrass at first harvest. Dry matter yield of bahiagrass was higher than that of bermudagrass (p<0.05). Peak forage DM production of bermudagrass and bahiagrass cultivars was in June and July, respectively. The content of crude protein (CP) and total digestibility nutrient (TDN) of bermudagrass cultivars were higher than those of bahiagrass during the first harvest. Acid detergent fiber (ADF) and in vitro DM digestibility (IVDMD) were similar across the four cultivars. In Jeju, bermudagrass and bahiagrass provide a useful option for supplemental summer forage in most livestock forage systems.