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Effects of Applying Microbial Additive Inoculants to Spent Mushroom Substrate (Flammulina velutipes) on Rumen Fermentation and Total-tract Nutrient Digestibility in Hanwoo Steers (팽이버섯 부산물 발효에 따른 한우 거세우 반추위 성상 및 소화율에 미치는 영향)

  • Baek, Youl-Chang;Jeong, Jin-Young;Oh, Young-Kyoon;Kim, Min-Seok;Lee, Sung-Dae;Lee, Hyun-Jeong;Do, Yoon-Jung;Ahmadi, Farhad;Choi, Hyuck
    • Korean Journal of Organic Agriculture
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    • v.25 no.3
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    • pp.569-586
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    • 2017
  • We inoculated a spent mushroom substrate from Flammulina velutipes (SMSF) with a microbial additive and assessed the effects on chemical composition, ruminal fermentation parameters, and total-tract nutrient digestibility. In Exp. 1, three cannulated Hanwoo steers were used in an in situ trial to determine the degradation kinetics of dry matter (DM) and crude protein (CP). In Exp. 2, three Hanwoo steers were randomly assigned to experimental diets according to a $3{\times}3$ Latin square for a 3-week period (2 weeks for adaptation and 1 week for sample collection). Experimental diets included the control diet (3.75 kg/d formulated concentrate mixture + 1.25 kg/d rice straw), SMSF diet (3.19 kg/d formulated concentrate mixture + 1.25 kg/d rice straw + 0.56 kg/d SMSF), and inoculated SMSF (ISMSF) diet (3.19 kg/d formulated concentrate mixture + 1.25 kg/d rice straw + 0.56 kg/d ISMSF). The chemical composition of ISMSF did not differ from that of SMSF. Microbial additive inoculation decreased pH (P<0.05) and improved preservation for SMSF. The percentages of DM, neutral detergent fiber (NDF), and acid detergent fiber (ADF) in ISMSF were slightly lesser than those in SMSF. Ruminal fermentation characteristics and total-tract nutrient digestibility were not affected by diet. Overall, microbial additive inoculation improved preservation for SMSF and may allow improved digestion in the rumen; however, the total digestible nutrients (TDN) of SMSF and ISMSF diets were slightly lesser than the control diet. The ISMSF can be used as an alternative feedstuff to partially replace formulated concentrate feed.

Characterization of Tobacco mosaic virus Isolated fromSolanum tuberosum ‘Chubak’ in Korea (감자 '추백' 에 발생한 Tobacco mosaic virus 의 특성)

  • Kim, Joung-Soo;Kim, Jae-Hyun;Choi, Gug-Seoun;Chae, Soo-Young;Kim, Hyun-Ran;Joung, Bong-Nam;Choi, Yong-Mun
    • Research in Plant Disease
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    • v.9 no.2
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    • pp.89-93
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    • 2003
  • An isolate of Tobacco mosaic virus (TMV) was isolated from potato cultivar ‘Chubak’ showing vein clearing and mild mosaic at Namhae in Korea. This isolate, TMV-St, was differentiated from other tobamoviruses based on biological properties, serological relationships and nucleotide sequence analyses of coat protein genes. TMV-St caused typical symptoms on four indicator plants as compared to the tobamovirus of TMV-U1, Pepper mild mottle virus (PMMoV), and Tomato mosaic virus (ToMV), which caused economic losses in Solanaceous vegetables, tomato, pepper, and eggplant. Remarkably, the TMV-St induced distinctly different symptom of systemic chlorotic spots on Chenophodium murale. On C. murale, Gomphorena globosa, and Nic-otiana rustica, the four viruses were classed by the virulence of systemic or local infections. In serological test TMV-St antiserum showed a precipitation line with each other tabamovirus. The CP gene of TMV-St contain 477 nucleotides, and the nucleotides sequence was the most similar to that of TMV-U1.

Effect of Conjugated Linoleic Acid Feeding on the Growth Performance and Meat Fatty Acid Profiles in Broiler: Meta-analysis

  • Cho, Sangbuem;Ryu, Chaehwa;Yang, Jinho;Mbiriri, David Tinotenda;Choi, Chang-Weon;Chae, Jung-Il;Kim, Young-Hoon;Shim, Kwan-Seob;Kim, Young Jun;Choi, Nag-Jin
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.7
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    • pp.995-1002
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    • 2013
  • The effect of conjugated linoleic acid (CLA) feeding on growth performance and fatty acid profiles in thigh meat of broiler chicken was investigated using meta-analysis with a total of 9 studies. Overall effects were calculated by standardized mean differences between treatment (CLA fed) and control using Hedges's adjusted g from fixed and random effect models. Meta-regression was conducted to evaluate the effect of CLA levels. Subgroups in the same study were designated according to used levels of CLA, CP levels or substituted oils in diets. The effects on final body weight, weight gain, feed intake and feed conversion ratio were investigated as growth parameters. Total saturated and unsaturated fatty acid concentrations and C16:0, C18:0, C18:2 and C18:3 concentrations in thigh meat of broiler chicken were used as fatty acid profile parameters. The overall effect of CLA feeding on final weight was negative and it was only significant in fixed effect model (p<0.01). Significantly lower weight gain, feed intake and higher feed conversion ratio compared to control were found (p<0.05). CLA feeding on the overall increased total saturated fatty acid concentration in broilers compared to the control diet (p<0.01). Total unsaturated fatty acid concentration was significantly decreased by CLA feeding (p<0.01). As for individual fatty acid profiles, C16:0, C18:0 and C18:3 were increased and C18:2 was significantly decreased by CLA feeding (p<0.01). In conclusion, CLA was proved not to be beneficial for improving growth performance, whereas it might be supposed that CLA is effective modulating n-6/n-3 fatty acids ratio in thigh meat. However, the economical compensation of the loss from suppressed growth performance and increased saturated fatty acids with the benefit from enhanced n-6/n-3 ratio should be investigated in further studies in order to propose an appropriate use of dietary CLA in the broiler industry.

Influence of Functionalization of Silica with Ionic Liquid on Ethylene Polymerization Behavior of Supported Metallocene (실리카의 이온성 액체 기능화가 메탈로센 담지촉매의 에틸렌 중합 거동에 미치는 영향)

  • Lee, Jeong Suk;Lee, Chang Il;Ko, Young Soo
    • Applied Chemistry for Engineering
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    • v.27 no.1
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    • pp.86-91
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    • 2016
  • Three amorphous silicas and SBA-15 were employed as supports, which were capable of confining ionic liquid (IL) and metallocene in the nanopore. Ionic liquid functionalized silica was prepared by the interaction between the chloride anions of 1,3-bis(cyanomethyl)imidazolium chloride and the surface OH groups. Metallocene and methylaluminoxane (MAO) were subsequently immobilized on the ionic liquid functionalized silica for ethylene polymerization. The metallocene supported on ionic liquid functionalized XPO-2412 and XPO-2410 having a larger pore diameter compared to SBA-15 showed higher activity than that of using supported catalyst without ionic liquid functionalization. However, the activity of metallocene supported on SBA-15 decreased after ionic liquid functionalization, suggesting that the diffusion of ethylene monomer and cocatalyst to the active site of nanopore was restricted during ethylene polymerization. This could be resulted from significant reduction of the pore diameter due to the immobilization of ionic liquid and $(n-BuCp)_2ZrCl_2$ and MAO. The effect on polymerization activity in accordance with the concentration of hydroxyl groups on the surface was also investigated. The polymerization activity increased as the concentration of hydroxyl groups on amorphous silica increased. The polymerization activities of metallocene supported on silica showed the similar trend after ionic liquid functionalization.

Study on Summer Forage Crop Cultivation Using SCB (Slurry Composting-Biofilteration) Liquid Fertilizer on Reclaimed Land (간척지에서 SCB 액비를 이용한 여름철 사료작물 재배에 관한 연구)

  • Jo, Nam-Chul;Shin, Jae-Soon;Kim, Sun-Ho;Yoon, Sei-Hyung;HwangBo, Soon;Jung, Min-Woong;Lee, Kyung-Dong;Kim, Won-Ho;Seo, Sung;Kim, Jong-Geun;Song, Chae-Eun;Choi, Ki-Choon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.30 no.2
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    • pp.121-126
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    • 2010
  • Until now, The experiment about the forage crop have been almost not conducted on the reclaimed land. Therefore, this experiment was carried out in order to know productivity of summer forage crop using slurry composting-biofilteration (SCB) liquid fertilizer on reclaimed land of Hwaong and Sukmoon in korea from 2008 to 2009. The forage crops used in this experiment were corn and sorghum${\times}$sorghum hybrid which are used as summer forage crops in South Korea. The experiment was treated with chemical fertilizer (CF), swine slurry (SS) and SCB liquid fertilizer. Dry matter (DM) yield of corn was higher than those of sorghum${\times}$sorghum hybrid in both reclaimed lands but the effect of SCB liquid fertilizer was not appeared. The neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents of corn were lower than those of sorghum${\times}$sorghum hybrid. The crude protein (CP) content and in vitro dry matter digestibility (IVDMD) of corn were higher than those of sorghum${\times}$sorghum hybrid. In generally feed values of corn were higher than those of sorghum${\times}$sorghum hybrid. The results of this study showed that summer forage crop cultivation using uses SCB liquid fertilizer on reclaimed land are possible.

Effect of Various Energy Regimens on Egg Production and Feed Cost of Broiler Breeders Peaked in Winter Season (동절기 산란피크 육용종계 산란기의 에너지 공급체계가 산란능력과 경제성에 미치는 영향)

  • 함영훈;김상인;이규호
    • Korean Journal of Poultry Science
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    • v.30 no.3
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    • pp.169-175
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    • 2003
  • This experiment was conducted to study the optimum energy feeding regimens fur broiler breeders peaked in winter season with 400 caged hens of Ross strain. Four energy supply regimens which were different in daily energy allotment during laying period were employed for 40 weeks from 24 to 64 weeks of age. All experimental diets were formulated to contain 2,750 kcal ME/kg with adjustments made in total feed allotment to provide the desired energy levels. Total consumption of the feed would provide 20 g of protein, 4 g of calcium and 0.35 g of available phosphorus. There were no difference in hen-day egg production and average egg weight among the regimens of energy supply. Feed, ME and feed cost required per egg or per kg egg were significantly increased as the level of energy allotment increased(p<0.05). It was concluded that the energy supply regimen, which supplied 280 kcal ME per day at the age of 24 weeks and then increased the energy supply up to 400 kcal ME per day at the peak period of 30∼34 weeks of age, was superior in feed, ME and feed cost required per egg or per kg egg without any adverse effect on eg production and egg weight.

Nutritional Evaluation of Rice with Different Processing Treatments on in vitro Rumen Fermentation Characteristics and in situ Degradation (재고미의 가공처리에 따른 in vitro, in situ 소화율 및 발효성상 평가)

  • Yang, Sung-Jae;Jung, Eun-Sang;Kim, Han-Been;Shin, Taek-Soon;Cho, Byung-Wook;Cho, Seong-Keun;Kim, Byeong-Woo;Seo, Ja-Kyeom
    • Korean Journal of Organic Agriculture
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    • v.26 no.2
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    • pp.281-296
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    • 2018
  • This study was conducted to evaluate the effect of different processing of rice on rumen fermentation in in vitro and in situ experiments. Different processing treatments (extruding, roasting, and steaming) were used in this study and all treatments were ground through a cyclone mill (Foss, Hillerød, Denmark) fitted with a 1 mm screen. Non-treated rice was considered to a control substrate. Then, all treatments were used in in vitro and in situ experiments. Total gas production and dry matter digestibility in control were lower than any other treatment at all incubation times (P<0.01). The lowest ammonia nitrogen ($NH_3-N$) concentration was observed in control among treatments at 6, 12, and 24 h incubation (P<0.01). Extruding had a highest total volatile fatty acids (VFA) concentration at 6, 12 h incubation (P<0.01) and Steaming exhibited a highest total VFA at 24 h (P<0.01). The lowest total VFA concentration was observed in control at 6, 12, and 24 h (P<0.01). In an in situ, The highest value of soluble fraction, degradation rates, effective degradability was observed in extruding (P<0.01). It was considered that feed processing increased dry matter digestibility, total VFA concentration, and decreased pH as well as $NH_3-N$ concentration indicating that processing may increase nutrient degradation of rice in the rumen.

Characteristics of Chlorination Byproducts Formation of Amino Acid Compounds (아미노산 성분에서의 염소 소독부산물 생성 특성)

  • Son, Hee-Jong;Choi, Young-Ik;Bae, Sang-Dae;Jung, Chul-Woo
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.5
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    • pp.332-340
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    • 2009
  • This study was conducted to analyze and determine formation potentials for chlorination disinfection by-products (DBPs) from twenty amino acid compounds with or without $Br^-$. Two of twenty amino acid compound were tryptophan and tyrosine that were relatively shown high for formation of trihalomethanes (THMs)/dissolved organic carbon (DOC) whether or not $Br^-$ presented. Other 18 compounds were shown low for formation of THMs/DOC whether or not $Br^-$ presented. Five amino acid compounds that were tryptophan, tyrosine, asparagine, aspartic acid and histidine were shown high for formation of haloacetic acids (HAAs)/DOC whether or not $Br^-$ presented. Although formation of dichloroacetic acid (DCAA) was dominated in asparagine, aspartic acid and histidine, trichloroacetic acid (TCAA) was dominated in tryptophan and tryptophan. The formation of haloacetnitriles (HANs)/DOC whether or not $Br^-$ presented was high in Aspartic acid, histidine, asparagine, tyrosine and tryptophan. Specially, aspartic acid was detected 660.2 ${\mu}$g/mg (HAN/DOC). Although the formation of chloralhydrate (CH)/DOC was shown high in asparagine, aspartic acid, histidine, methionine, tryptophan and tyrosine, the formation of Chloropicrin (CP)/DOC was low (1 ${\mu}$g/mg) in twenty amino acid compounds. The formations of THM, HAA and HAN were also investigated in functional groups of amino acids. The highest formation of THM was shown in amino acids compounds (tryptophan and tyrosine) with an aromatic functional group. Highest, second-highest, third-highest and fourth-highest functional groups for formation of HAA were aromatic, neutral, acidic and basic respectively. In order of increasing functional groups for formation of HAN were acidic, basic, neutral and aromatic.

COMPARISON OF RIGIDITY AND CASTABILITY IN DIFFERENT DESIGNS OF MAXILLARY MAJOR TITANIUM FRAMEWORK (타이타늄 상악 주연결장치에 디자인에 따른 주조성 및 견고성 비교)

  • Lee, Young-Jae;Vang, Mong-Sook;Yang, Hong-So;Park, Sang-Won;Park, Ha-Ok;Lim, Hyun-Pil
    • The Journal of Korean Academy of Prosthodontics
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    • v.45 no.4
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    • pp.431-443
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    • 2007
  • Statement of problem: Injuries along with discomfort may result on the oral mucosa when non-rigid material is used as the major connector in construction of RPD, since nonrigid major connectors transmit unstable forces throughout the appliance. Titanium which recently draws attention as a substitute of Co-Cr had a difficulty in fabricating due to high melting temperature but the development of casting technique makes it possible to apply to the clinical case. Purpose: The purpose of this study was to investigate the rigidity and the castability of titanium upper major connector by design and make a comparison with Co-Cr major connectors which are widely used in clinical cases now. Material and methods: Casting was done using CP-Ti(Grage 2) (Kobe still Co., Japan) for the experimental groups, and 4 various designs namely palatal strap, U-shaped bar, A-P strap, and complete palatal plate were casted and 5 of each designs were included in each group. For the experimental group, Universal testing machine (Model 4502; Instron, Canton, Mass) was used to apply vertical torsional force vertically to the horizontal plane of major connector. In the second experiment, Vertical compressive force was applied to the horizontal plane of major connector. As a comparative group, Co-Cr major connector was equally manufactured and underwent the same experimental procedures Strain rate was measured after constant loading for one minute duration, and statistical analysis was done with SPSS ver.10.0 for WIN(SPSS. Inc. USA). From the one-way ANOVA and variance analysis (P=0.05), Scheffe's multiple comparison test implemented. Results: 1. Least amount of strain was observed with complete palatal plate followed by A-P bar, palatal bar, and the U-shaped bar having most amount of strain. 2. In all designs of titanium major connector, less strain rate was observed under compressive loading than under torsional loading showing more resistance to lateral force. 3. For titanium major connector, less strain rate was observed when the force is applied to the first premolar area rather than to the second molar area indicating more strength with shorter length of lever. 4. In Comparison of Co-Cr major connector with titanium major connector, palatal strap and U-shaped bar designs showed higher strength under torsional force that is statically significant, and under compressive force, no significant difference was observed expert for U-shaped bar. 5. In titanium major connector, complete palatal plate showed lowest success rate in casting when compared with the Co-Cr major connector. Conclusion: Above results prove that when using titanium for major connector, only with designs capable of generating rigidity can the major connector have almost equal amount of rigidity as Co-Cr major connector and show lower success rate in casting when compared with the Co-Cr major connector.

A Study on Cost Estimation of Spatial Query Processing for Multiple Spatial Query Optimization in GeoSensor Networks (지오센서 네트워크의 다중 공간질의 최적화를 위한 공간질의처리비용 예측 알고리즘 연구)

  • Kim, Min Soo;Jang, In Sung;Li, Ki Joune
    • Spatial Information Research
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    • v.21 no.2
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    • pp.23-33
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    • 2013
  • W ith the recent advancement of IoT (Internet of Things) technology, there has been much interest in the spatial query processing which energy-efficiently acquires sensor readings from sensor nodes inside specified geographical area of interests. Therefore, various kinds of spatial query processing algorithms and distributed spatial indexing methods have been proposed. They can minimize energy consumption of sensor nodes by reducing wireless communication among them using in-network spatial filtering technology. However, they cannot optimize multiple spatial queries which w ill be w idely used in IoT, because most of them have focused on a single spatial query optimization. Therefore, we propose a new multiple spatial query optimization algorithm which can energy-efficiently process multiple spatial queries in a sensor network. The algorithm uses a concept of 'query merging' that performs the merged set after merging multiple spatial queries located at adjacent area. Here, our algorithm makes a decision on which is better between the merged and the separate execution of queries. For such the decision making, we additionally propose the cost estimation method on the spatial query execution. Finally, we analyze and clarify our algorithm's distinguished features using the spatial indexing methods of GR-tree, SPIX, CPS.