• Asian-Australasian Journal of Animal Sciences
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• 제15권3호
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• pp.390-395
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• 2002

Two experiments were conducted to study the performance and carcass characteristics of Hanwoo (Korean native beef cattle) steers (Experiment 1) and the nutrient digestibility of sheep (Experiment 2) when the animals fed diets containing four levels of dried wormwood (Artemisia sp.). For both experiments the animals were given a basal diet consisting of rice straw and concentrate mixed at 3:7 ratio (on DM basis). In Experiment 1, the treatments were designed as a completely randomized design with two feeding periods. Steers were allotted in one of four dietary treatments, which were designed to progressively substitute dried wormwood for 0, 3, 5 and 10% of the rice straw in the basal diet. Bodyweight gain and average daily gain (ADG) of Hanwoo steers fed diets containing the 5 and 10% wormwood inclusion were greater (p<0.05) than the 3% wormwood-feeding group. Total and daily intakes of roughage and concentrate were not altered by all levels of dried wormwood inclusion. However, the 10% dried wormwood inclusion led to increased total feed intake (p<0.05) compared with that of the 0 and 3% of dried wormwood inclusion. Carcass weight, carcass yield and backfat thickness were not altered by dried wormwood inclusion. The highest level of dried wormwood inclusion resulted in significantly increased (p<0.05) loin-eye area compared with the 0 and 5% levels of dried wormwood inclusion. Experiment 2 was designed by a $4{\times}4$ Latin square with four periods. Sheep were allocated in one of four dietary treatments as same as in Experiment 1. Digestibilities of DM and TDN were significantly increased (p<0.05) in sheep fed the diet containing all three levels of dried wormwood inclusion compared with the control treatment. Digestibilities of CP and crude fiber in the 5% dried wormwood inclusion highly increased (p<0.05) up to 8.2 and 5.5% respectively relative to the control treatment. The palatability was significantly improved (p<0.05) by the 5% dried wormwood inclusion compared with the control treatment. It is concluded that feeding diets containing dried wormwood substituted for equal weights of rice straw at 5 and 10% levels would be anticipated to provide better quality roughage for beef cattle production and higher income for beef cattle producers.

• Asian-Australasian Journal of Animal Sciences
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• 제23권8호
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• pp.1066-1072
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• 2010

In this study, the effects of replacing inorganic copper, zinc and manganese with different levels of organic complexes of the same trace minerals on the lipid peroxidation and antioxidant defense systems in broilers were investigated. Two-hundred Ross-308 one-day-old broiler chickens were placed on controlled diets until 42 d of age. The experimental animals were divided into four groups comprising three experimental groups and one control group, each consisting of 50 chickens. All groups were also divided into five subgroups each containing 10 broiler chicks. The mineral content of the control group diet was controlled using a standard inorganic mineral premix with supplement levels and sources of trace minerals typical of commercial broiler diets according to the National Research Council (NRC) (containing 8 mg Cu as $CuSO_4$, 40 mg Zn as $ZnSO_4$, and 60 mg Mn as MnO, per kg). In the experimental diets, mineral premix was also comprised of inorganic formulations, except for those of Cu, Zn and Mn. Organically-complexed Cu, Zn, and Mn were separately added to the basal diet at 1/3 (L1), 2/3 (L2) and 3/3 (L3) levels with respect to the NRC recommendation, as Bioplex $Cu^{TM}$, Bioplex $Zn^{TM}$, Bioplex $Mn^{TM}$. At the end of the trial, the plasma Zn level significantly increased when the plasma Cu level significantly decreased (p<0.05) in chickens fed at 2/3 and 3/3 levels of organically complexed minerals. The liver trace mineral concentrations were significantly higher in chickens fed inorganic trace minerals in comparison to those fed organically-complexed minerals. The plasma malondialdehyde (MDA) level of experimental chickens was decreased in groups receiving levels of organic Cu, Zn and Mn in comparison to those fed inorganic forms (p<0.01). The erythrocyte superoxide dismutase (SOD) activity was higher in all groups receiving the organic mineral supplements in comparison to those fed inorganic forms (p<0.01). No differences were observed on either the erythrocyte catalase (CAT) activity or the plasma ceruloplasmin (Cp) levels, and the liver MDA levels and liver CAT and SOD activities in any of the groups that received the organic supplements of Cu, Zn, and Mn. It was concluded that supplementation of lower levels of organically-complexed copper, zinc, and manganese instead of their inorganic forms in diets had no negative effects on the antioxidant defense system in broilers.

• Asian-Australasian Journal of Animal Sciences
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• 제21권2호
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• pp.204-213
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• 2008

Four ruminally cannulated Holstein cows averaging 43 days in milk (DIM) were used in a $4{\times}4$ Latin square to determine the effect of feeding extruded soybean, ground canola seed and whole cottonseed on ruminal fermentation and milk fatty acid profile. One hundred and twenty lactating Holstein cows, 58 (${\pm}31$) DIM, were assigned to four treatments in a completely randomized block design to study the effects of the three types of oilseeds on production parameters and milk fatty acid profile. The four diets were a control diet (CON) and three diets in which 10% extruded soybean (ESB), 5% ground canola seed (GCS) and 10% whole cottonseed (WCS) were included, respectively. Diets consisted of concentrate mix, corn silage and Chinese wild rye and were balanced to similar concentrations of CP, NDF and ADF. Ruminal fermentation results showed that ruminal fermentation parameters, dry matter intake and milk yield were not significantly affected by treatments. However, compared with the control, feeding cows with the three oilseed diets reduced C14:0 and C16:0 and elevated C18:0 and C18:1 concentrations in milk, and feeding ESB increased C18:2 and cis9, trans11 conjugated linoleic acid (CLA). Production results showed that feeding ESB tended to increase actual milk yield (30.85 kg/d vs. 29.29 kg/d) and significantly decreased milk fat percentage (3.53% vs. 4.06%) compared with CON. Milk protein (3.41%) and solid non-fat (13.27%) from cows fed WCS were significantly higher than from cows fed CON (3.24% and 12.63%, respectively). Milk urea N concentrations from cows fed the ESB (164.12 mg/L) and GCS (169.91 mg/L) were higher than cows fed CON (132.31 mg/L). However, intake of DM, 4% fat corrected milk, energy corrected milk, milk fat and protein yields, milk lactose percentage and yield, somatic cell count and body condition score were not affected by different treatments. The proportion of medium-chain fatty acid with 14 to 16 C units in milk was greatly decreased in cows fed ESB, GCS and WCS. Feeding ESB increased the concentration in milk of C18:1, C18:2, C18:3 and cis9, trans11-CLA content by 16.67%, 37.36%, 95.24%, 72.22%, respectively, feeding GCS improved C18:0 and C18:1 by 17.41% and 33.28%, respectively, and feeding WCS increased C18:0 by 31.01% compared with feeding CON. Both ruminal fermentation and production trial results indicated that supplementation of extruded soybean, ground canola seed and whole cottonseed could elevate the desirable poly- and monounsaturated fatty acid and decrease the medium chain fatty acid and saturated fatty acid content of milk fat without negative effects on ruminal fermentation and lactation performance.

• KSBB Journal
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• 제29권6호
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• pp.443-447
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• 2014

Biosynthesis of lactate-containing polyhydroxyalkanoates (PHAs) was examined in recombinant Escherichia coli W strain from sucrose. The Pseudomonas sp. MBEL 6-19 phaC1437 gene and the Clostridium propionicum pct540 gene, which encode engineered Pseudomonas sp. MBEL 6-19 PHA synthase 1 ($PhaC1_{Ps6-19}$) and engineered C. propionicum propionyl-CoA transferase ($Pct_{Cp}$), respectively, were expressed in E. coli W to construct key metabolic pathway to produce poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)]. The recombinant E. coli W expressing the phaC1437 gene and the pct540 gene could synthesize P(3HB-co-13mol%LA) up to the polymer content of 31.3 wt% when it was cultured in chemically defined MR medium containing 20 g/L of sucrose and 2 g/L of sodium 3-hydroxybutyrate. When Ralstonia eutropha phaAB genes were additionally expressed to provide 3-hydroxybutyrate-CoA (3HB-CoA) from sucrose, P(3HB-co-16mol%LA) could be synthesized from sucrose as a sole carbon source without supplement of sodium 3-hydroxybutyrate in culture medium, but the PHA content was decreased to 12.2 wt%. The molecular weight of P(3HB-co-16 mol%LA) synthesized in E. coli W using sucrose as carbon source were $1.53{\times}10^4$ ($M_n$) and $2.78{\times}10^4$ ($M_w$), respectively, which are not different from those that have previously been reported by other recombinant E. coli strains. Engineered E. coli strains developed in this study should be useful for the production of lactate-containing PHAs from sucrose, one of the most abundant and least expensive carbon sources.

• 한국조리학회지
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• 제21권2호
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• pp.52-59
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• 2015

본 연구는 사물탕을 첨가한 데리야끼 소스의 품질 특성을 측정하여 기능성 식품으로 개발하고자 수행되었다. 사물탕을 첨가한 데리야끼 소스의 가용성 고형분 함량은 $58.33{\pm}1.15{\sim}76.00{\pm}4.58(%)$였으며, 염도는 $0.38{\pm}0.20{\sim}60.63{\pm}0.36(%)$으로 수용성 고형분 및 염도 모두 사물탕 첨가량이 늘어남에 따라 유의적으로 감소된 값을 나타냈다. pH는 $4.16{\pm}0.12{\sim}4.26{\pm}0.12$로 첨가량에 따라 그 값이 증가하였으나 유의적인 차이는 나타나지 않았고, 적정산도는 $2.00{\pm}0.02{\sim}2.13{\pm}0.03$의 값으로 대조구 및 5% 첨가구 사이의 차이는 나타나지 않았으며, 10% 이상 첨가시 그 값이 증가함을 보였다. 점도 측정 결과, $21.53{\pm}1.88{\sim}44.40{\pm}0.80(cp)$으로, 사물탕 첨가량이 증가함에 따라 데리야끼 소스의 점도값이 낮아졌다. 전자공여능 측정 결과, $25.72{\pm}1.52{\sim}32.94{\pm}0.94(%)$로 사물탕 첨가량을 증가시 킴에 따라 유의적으로 값이 높아졌으나, 5% 첨가구와 대조구 사이의 차이는 나타나지 않았으며, 10%와 15% 첨가구 사이의 유의적인 차이는 나타나지 않았다. 기호도 평가 결과, 향, 단맛, 짠맛에 대한 평가결과 대조구보다 첨가구의 값이 낮았으며, 데리야끼 소스에서 인지되는 사물탕의 향과 맛을 평가한 결과, 10% 첨가구에서 높은 기호도를 나타냈고, 사물탕 맛에서도 사물탕 첨가구에서 무첨가구보다 높은 기호도를 나타냈다. 전반적 기호도는 15% 첨가구에서만 비교적 낮은 값을 나타내, 사물탕을 10% 수준으로 데리야끼 소스에 첨가하는 것은 기능성을 부여한 식품으로서 그 사용 가능성이 높다고 생각된다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6261-6265
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• 2013

Background: Recent studies have suggested that expression of the RAS protein activator like-1 gene (RASAL1) is decreased in gastric carcinoma tissues and cell lines, indicated a role in tumorigenesis and development of gastric cancer. Reduced expression of RASAL1 could result in aberrant increase of activity of RAS signaling pathways in cancer cells. However, the exact mechanism which induces down-regulation of the RASAL1 gene remains unclear. This study aimed to determine the methylation status and regulation of RASAL1 in gastric cancer. Materials and Methods: Using the methylation-specific polymerase chain reaction (MSP), the methylation status of CpG islands in the RASAL1 promoter in gastric cancers and paired adjacent non-cancerous tissues from 40 patients was assessed and its clinicopathological significance was analyzed. The methylation status of RASAL1 in gastric cancer lines MKN-28, SGC-790l, BGC-823, as well as in normal gastric epithelial cell line GES-l was also determined after treatment with a DNA methyltransferase inhibitor, 5-aza-2'-doexycytidine (5-Aza-CdR). RAS activity (GAS-GTP) was assessed through a pull-down method, while protein levels of ERK1/2, a downstream molecule of RAS signaling pathways, were determined by Western blotting. Results: The frequencies of RASAL1 promoter methylation in gastric cancer and paired adjacent non-cancerous tissues were 70% (28/40) and 30% (12/40) respectively (P<0.05). There were significantly correlations between RASAL1 promoter methylation with tumor differentiation, tumor size, invasive depth and lymph node metastasis in patients with gastric cancer (all P<0.05), but no correlation was found for age or gender. Promoter hypermethylation of the RASAL1 gene was detected in MKN-28, SGC-790l and BGC-823 cancer cells, but not in the normal gastric epithelial cell line GES-1. Elevated expression of the RASAL1 protein, a decreased RAS-GTP and p-ERK1/2 protein were detected in three gastric cancer cell lines after treatment with 5-Aza-CdR. Conclusions: Aberrant hypermethylation of the RASAL1 gene promoter frequently occurs in gastric cancer tissues and cells. In addition, the demethylating agent 5-Aza-CdR can reverse the hypermethylation of RASAL1 gene and up-regulate the expression of RASAL1 significantly in gastric cancer cells in vivo. Our study suggests that RASAL1 promoter methylation may have a certain relationship with the reduced RASAL1 expression in gastric cancer.

• Asian-Australasian Journal of Animal Sciences
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• 제15권9호
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• pp.1334-1340
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• 2002

A 4 week experiment was designed to study the effects of replacing corn with Chinese brown rice or adding different amylase in brown rice basal diet on growth performance and apparent fecal digestibilities of nutrients in growing pigs. One hundred and eight cross-bred pigs (Duroc${\times}$Landrace${\times}$Large White), weighing an average of $18.35{\pm}0.12kg$, were randomly assigned to 6 treatments with 6 replications per treatment. Diet in treatment 1 was corn-soybean meal basal diet, and in treatment 2, 3 and 4, corn was replaced by brown rice on rates of 33.3%, 66.7% and 100% respectively on the basis of treatment 1. And diets in treatment 5 and 6 were similar to treatment 4 except two kinds of amylases, glucoamylase and ${\alpha}$-amylase, were added respectively. The brown rice used in this experiment was husked from one kind of early, long grain, non-glutinous rice (ELGNR, indica rice) in southern China. The results indicated that there was a slight improvement in growth performance of pigs in brown rice treatments (p>0.05). The blood urea nitrogen value in treatment 2 was lower than that in treatment 1 (p<0.05). The differences of apparent fecal digestibilities of most nutrients were significant (p<0.05) except CP. Digestibilities of GE, OM and DM in treatment 4 were the best and digestibility of crude fat in treatment 5 appeared best (p<0.05). Contrast results between treatment 1 and treatment 2 to 4 indicated that the digestibility of GE, OM and DM increased significantly with the replacing rates of brown rice (p<0.05). Contrast results between treatment 4 and 5 indicated that adding glucoamylase in brown rice diet increased growth performance slightly (p>0.05) but not for digestibilities. This experiment shows a positive effect of brown rice on growth performance, especially on nutrient digestibility.

• Asian-Australasian Journal of Animal Sciences
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• 제15권12호
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• pp.1754-1759
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• 2002

A study was carried out to investigate the nutritive value and utilization of hybrid sorghum and perennial grass species viz. setaria (Setaria anceps) and hybrid napier when intercropped with soybean by growing Jersey crossbred heifers. Fifteen growing crossbred heifers (Jersey${\times}$Red Sindhi) of between 7-10 months age and pre-trial average body weight of 49-50 kg were divided on the basis of weight in to three treatment groups viz. $T_1$-hybrid sorghum+soybean, $T_2$-setaria+soybean and $T_3$-hybrid napier+soybean in a completely randomized block design. Intercropped forages were harvested fresh, chaffed and mixed before they were offered to the heifers. Chemical composition of the herbage, dry matter intake (DMI), body weight gain and nutrient digestibility co-efficients were estimated. The herbage mixtures had crude protein (CP) content in the range of 11.87 to 13.86% and ether extract (EE) contents were 2.91 to 3.11%, respectively. The herbage mixtures were rich in minerals (ash). The gross energy (kcal/g DM) was higher in hybrid napier+soybean, while hybrid sorghum+soybean and setaria+soybean herbage mixtures had lower value for gross energy. The hybrid sorghum+soybean and setaria+soybean herbage mixtures had higher contents of NDF, ADF, cellulose, lignin and silica as compared to that of hybrid napier+soybean herbage mixture. The heifers fed hybrid napier+soybean herbage mixture had significantly (p<0.05) higher $DMI\;g/kg\;W^{0.75}$ ($97.41{\pm}4.34$) as compared to hybrid sorghum+soybean ($88.31{\pm}2.66$) and setaria+soybean ($79.29{\pm}1.06$) herbage mixtures. Nutrients digestibility, DCP percent, DCP intake and nitrogen balance were significantly (p<0.05) higher in the heifers fed on hybrid napier+soybean herbage mixture. There was a significant (p<0.05) difference among different herbage mixtures in TDN. The heifers on setaria+soybean herbage mixture had lower average body weight gain (g/day) than those on hybrid sorghum+soybean and hybrid napier+soybean herbage mixtures. Data obtained in this experiment demonstrated that herbage mixture of hybrid napier+soybean was better than hybrid sorghum+soybean and setaria+soybean herbage mixtures in the nutrition of growing heifers. It had highest nutritive value, better digestibility co-efficients which showed better growth rate and higher feed efficiency. In ranking, hybrid napier+soybean herbage mixture was better followed by hybrid sorghum+soybean and setaria+soybean in nutritive value in the parameters studied. For future wasteland development program in humid-sub tropics of Himachal Pradesh hybrid napier and its intercropping with soybean is recommended for general adoption because of its better adaptability and higher nutritive value.

• Asian-Australasian Journal of Animal Sciences
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• 제23권2호
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• pp.246-252
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• 2010

In order to improve the availability of phytase and probiotics together, a phytase gene from Aspergillus ficuum has been expressed in Lactobacillus. In this study, the transformed Lactobacillus with phytase gene was fed to pigs to determine its effect on pig production, feed conversion and gut microbes. Forty eight, 60-day-old, castrated pigs (Duroc${\times}$Landrace${\times}$Pietrain) were assigned to 6 groups, 8 pigs for each group. Group 1 was the control, group 2 was added with chlortetracycline (500 mg/kg), group 3 was added with the transformed Lactobacillus (500 mg/kg) with 20% (w/w) of calcium monohydrogen phosphate (CMP, $CaHPO_{4}$) removed, group 4 was added with the natural Lactobacillus (500 mg/kg) with 20% (w/w) of CMP removed, group 5 was added with the transformed Lactobacillus (500 mg/kg) with 40% (w/w) of CMP removed, group 6 was added with phytase (500 mg/kg) with 40% (w/w) of CMP removed. The results showed: i) the average daily gain (ADG) was improved in groups 2, 3 and 4 (p<0.05); ii) the diarrhea rates in the groups added with Lactobacillus were lower than in the other groups (p<0.05), in which the transformed Lactobacillus had more effect on reducing digestive disease; iii) the transformed Lactobacillus was most effective in improving the digestibilities of crude protein (CP), calcium (Ca), phosphorus (P), compared with the other groups (p<0.05); iv) Lactobacillus could increase lactic acid bacterium number and ammonia concentrations, and decrease pH values and E. coli number in pig feces (p<0.05); v) the phytase activity in the feces of pigs fed with the transformed Lactobacillus was 133.32 U/g, which was higher than in group 4 (9.58 U/g, p<0.05), and was almost the same as group 6 (135.94 U/g); vi) the transformed Lactobacillus could increase serum concentrations of IgA, triglyceride, and glutamic oxaloacetic transaminase activity (p<0.05), and had no significant effect on other serum indexes (p>0.05).

• 한국인지과학회:학술대회논문집
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• 한국인지과학회 2000년도 춘계 학술대회
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• pp.303-308
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• 2000

용언은 그 어간이 여러 문법소와 결합하면서 자동적 음운 변동을 제외한 형태 변동이 있는가, 없는가에 의해 규칙 용언/불규칙 용언으로 구분할 수 있다. 이러한 불규칙 용언은 심성 어휘집에 어떤 형태로 저장되어 있으며, 규칙 용언과는 어떠한 관계가 있는지, 나아가 실어증 환자의 경우에는 정상인에 비해 어떤 행동장애를 보이며, 장애가 있다면 어느 경로의 손상으로 인한 장애인지를 알아보는 것이 본 연구의 목적이다. 이를 위해 이해성 실어증 환자 한 명과 음어적 실행증 현상을 동반한 경미한 정도의 실어증 환자를 피험자로 하였다. 실험 과제는 단어 채워 넣기 과제(word completion task)를 사용하였다. 즉 주어진 기본형 용언을 검사 문장의 문맥에 맞게 활용하여 채워 넣는 것이다. 실험 결과에 의하면 환자들은 규칙용언의 활용(예. 먹다/먹는)과 불규칙 용언 중 형태를 유지한 채로 활용하는 경우(예. 줍다/줍고)에는 거의 오류가 없었으나, 불규칙 용언이 형태 변화를 겪어야 할 경우(예. 줍다/주워)에는 대부분 오류를 보였다. 또 이때는 기본형(basic form)을 그대로 유지하는 오류 방향성을 관찰할 수 있었다. 이는 그간 문법으로 구분되어 오던 규칙 용언/불규칙 용언의 정보 처리보다는 형태 유지/형태 변화 정보 처리의 영향이 크다는 것을 알 수 있다. 특히 이해성 실어증 환자는 전체적인 오류율이 매우 높았는데, 규칙 용언의 경우에도 오류를 보였다. 이때, 용언의 어간에 해당하는 부분에는 오류가 없고, 뒤에 따르는 내용과의 관계를 파악해야 하는 문법 기능소, 즉 연결 어미에서 오류를 보여 정보의 유지, 통합에 문제가 있다는 기존의 연구와도 일치하는 결과를 나타냈다.환자는 시제 선어말 어미를 선택하는데도 어려움을 보임이 확인되었다. 실험 3 역시 실험 1과 실험2에서와 동일하게 처리의 어려움을 보였다. 이러한 실험 결과들은 국어의 존칭과 시제 선어말 어미가 통사부에서 구(XP)와 결합하여 새로운 구를 형성하는 통사적 접사로 해석할 수 있으며 Grodzinsky의 가설을 지지하는 결과를 보여 줌으로서 국어에서도 AgrP, TP, CP 사이의 통사적 위계가 있음을 뒷받침하는 증거가 된다.전처리한 Group 3에서는 IL-2와 IL-4의 수준이 유의성있게 억제되어 발현되었다 (p < 0.05). 이러한 결과를 통하여 T. denticola에서 추출된 면역억제 단백질이 Th1과 Th2의 cytokine 분비 기능을 억제하는 것으로 확인 되었으며 이 기전이 감염 근관에서 발견되는 T. denticola의 치수 및 치근단 질환에 대한 병인기전과 관련이 있는 것으로 사료된다.을 보였다. 본 실험 결과, $Depulpin^{\circledR}은{\;}Tempcanal^{\circledR}와{\;}Vitapex^{\circledR}$에 비해 높은 세포 독성을 보여주공 있으나, 좀 더 많은 임상적 검증이 필요할 것으로 사료된다.중요한 역할을 하는 것으로 추론할 수 있다.근관벽을 처리하는 것이 필요하다고 사료된다.크기에 의존하며, 또한 이러한 영향은 $(Ti_{1-x}AI_{x})N$ 피막에 존재하는 AI의 함량이 높고, 초기에 증착된 막의 업자 크기가 작을 수록 클 것으로 여겨진다. 그리고 환경의 의미의 차이에 따라 경관의 미학적 평가가 달라진 것으로 나타났다.corner$적 의도에 의한 경관구성의 일면을 확인