• Journal of Life Science
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• v.24 no.4
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• pp.428-436
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• 2014

Loop-mediated isothermal amplification (LAMP) technique relies on autocycling strand displacement DNA sysnthesis without template denaturation steps under isothermal conditions. LAMP has more advantages than modern PCR, as it requires only basic laboratory equipment like an isothermal water bath, oven, and heating cabinet. Hence, in this study, five random primers were designed with Streptococcus parauberis, shikimate kinase Arok gene sequences (Genbank accession number: CP0024711). Two primers were selected based on the ladder pattern. Other optimum reaction conditions like temperature, time, and sensitivity were established and confirmed with conventional SYBR-green PCR. Results confirmed that the designed random primers were species specific, without any non-target DNA amplification under isothermal conditions. Hence, this study suggests that LAMP techniques could be used in the diagnosis of fish pathogen, specifically S. parauberis.

• The Plant Pathology Journal
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• v.16 no.6
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• pp.297-305
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• 2000

Weeds are widely grown in the field and are infected by many viruses. A survey was conducted to identify viruses infecting weeds in Korea. Virus-infected weed samples including Rorippa indica (L.) Hiern, R. islandica (Oed.) Bord, Crepidiastrum denticulatum (Houtt.) Pak & Kawanno, Achyranthes japonica (Miq.) Nakai, and Chrysanthemum boreale (Makino) Makino were collected in Kyonggi Province. These weeds were grown in the greenhouse and were isolated on 10 test plants. Several virus isolates were isolated fron infected tissues and were further studied by host range assay, serological test, electron microscopy (EM), reverse transcription-polymerase chain reaction (RT-PCR) and sequencing. Each isolated virus strain was mechanically transmitted to weeds and various hosts including Nicotiana spp., Brassica spp., Vigna unguiculata, Capsicum annuum, and Cucumis sativus and showed systemic mosaic, vein clearing, necrosis, mottle, malformation, chlorosis, and/or death of host plants in some cases. Each virus was then purified using infected leaves and observed by EM. From these results three viruses were isolated and identified as Turnip mosaic virus (TuMV), Broad bean wilt virus (BBWV), and Cucumber mosaic virus (CMV). RT-PCR using virus-specific oligonucleotide primers and the cloning were conducted to determine the nucleotide sequences of coat proteins of the three viruses their amino acid sequence were deduced. The amino acid sequence homologies were about 92.7 to 99.7%, 96.2 to 97.7%, and 93.9 to 98.6% to other reported TuMV, BBWV, and CMV strains, respectively. These results suggest that many weeds may serve as primary inoculum source of diseases caused by TuMV, BBWV, CMV and that the management of these viral diseases can be achieved through weed control.

• The Plant Pathology Journal
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• v.36 no.1
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• pp.76-86
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• 2020

Cucumber mosaic virus (CMV) is damaging to the growth and quality of lettuce crops in Lanzhou, China. Recently, however, for the first time an isolate of lettuce necrotic yellows virus (LNYV) has been detected in lettuce crops in China, and there is concern that this virus may also pose a threat to lettuce production in China. Consequently, there is a need to develop a rapid and efficient detection method to accurately identify LNYV and CMV infections and help limit their spread. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed to detect the nucleoprotein (N) and coat protein (CP) genes of LNYV and CMV, respectively. RT-LAMP amplification products were visually assessed in reaction tubes separately using green fluorescence and gel electrophoresis. The assays successfully detected both viruses in infected plants without cross reactivity recorded from either CMV or LNYV or four other related plant viruses. Optimum LAMP reactions were conducted in betaine-free media with 6 mM Mg²⁺ at 65℃ for LNYV and 60℃ for 60 min for CMV, respectively. The detection limit was 3.5 pg/ml and 20 fg/ml using RT-LAMP for LNYV and CMV plasmids, respectively. Detection sensitivity for both RT-LAMP assays was greater by a factor of 100 compared to the conventional reverse transcription polymerase chain reaction assays. This rapid, specific, and sensitive technique should be more widely applied due to its low cost and minimal equipment requirements.

• Journal of Korean Physical Therapy Science
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• v.10 no.1
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• pp.38-46
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• 2003

Since the current tendencies show us the increasing number of cerebral palsy children and the standard longevity, we need to find out more research about the following various problems. Therefore I have tried to figure out the difference of oxygen saturation and heart rate between before ambulation and after. Objects chosen are 17 C.P children on the process of treatment those who were able to walk and 8 normal children in Ah-San hospital, Gang-Nung. They haven't had either any operation or suffered heart disease and I measured their oxygen saturation and heart rate by using pulse-oximeter and are analyzed by SPSS (10.07 version). Results are the followings; 1. There was no difference of oxygen saturation and heart rate between pre-ambulation and post-ambulation of normal and spastic hemiplegia children. 2. There showed the significant statistic difference of oxygen saturation, heart rate between pre-ambulates and post-ambulation of normal and spastic diplegia(p<.05). 3. There was no difference of oxygen saturation, heart rate in C.P between(p>.05), but shows the significant statistic difference in heart rate(p<.05). As I compared the oxygen saturation and heart rate of spastic and normal children on between pre-ambulation and post-ambulation, theres is significant statistic difference on both items (p<.05). However there was no difference of oxygen saturation among hemiplegia, normal and diplegia children while the pulse rate showed the significant difference(p<.05). According to this clinical research, CP children's oxygen saturation and heart rate had no change between pre and post compared to normal children. But there was difference in diplegia. This is why we need to invest time to study these kinds of research about various analysis and comparison of oxygen saturation and heart rate, and furthermore making use of pulse-oxymetry in physical therapy room for the children involved would be beneficial to calculate in accuracy without any discomfort for the patient as well.

• Journal of Embryo Transfer
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• v.21 no.1
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• pp.53-58
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• 2006

This study was conducted to comparing on vitrification of mouse oocytes and embryos using CPS, conventional straws and CPS by evaluating in morphological survival for oocytes, and embryonic cleavages and blastocyst formation for embryos. The morphological survival in vitro after thawing of vitrified oocytes using CPS (75%) and conventional straws (72%) were significantly higher (p<0.05) than that using OPS (68%). The blastocyst formation rates of vitrified embryos using CPS (48.6%) and unfrozen control embryos (56.0%) were significantly higher (p<0.05) than those of conventional straws (43.4%) and OPS (37.7%). The rates of morula formation were also higher to control, CPS, conventional straws and OPS in orderly. These results show that CPS has the advantages of achieving a high survival and safety preservation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.3
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• pp.245-250
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• 2015

This study was conducted from October 2014 to May 2015 to explore forage production and feed values of Italian ryegrass, Rye and whole crop barley as winter forage crops in the Southern region of Korea. The experimental location was over 10 points for each species and each sampling point area was $1m^2$ (Width: 1 m ${\times}$ Length: 1 m). Air mean temperature and rainfall in the Southern region of Korea during the experimental period was $6.95{\pm}5.75^{\circ}C$ and $70.45{\pm}54.68mm$, respectively. Fresh forage yield of Italian ryegrass, the most cultivated forage in the Southern region of Korea, was $44.4{\pm}7.0ton/ha$. The percentage of dry matter for whole crop barley was $28.9{\pm}7.0%$. Crude protein (CP) was higher in Italian ryegrass ($10.7{\pm}5.3%$) while total digestible nutrient (TDN) had the highest value in whole crop barley. Crude protein was not significantly different by location. However, the neutral detergent fiber (NDF), acid detergent fiber (ADF) and total digestible nutrient value of forage from Jeonbuk province were higher than in forage from Gyeongnam province.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.3
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• pp.195-202
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• 1993

This experiment was carried out to investigate the eft'ects of different seeding dates on the agronomic characteristics, forage yield and quality of fall sown cats(Avena sativa L.). The experiment was conducted at the forage experimental field, Seoul National University. Suweon in 1990. The results obtained are summarized as follows; 1. Present data indicated that plant and ear heights were decreased as the seeding date was delayed. Dry matter accumulation was increased due to the higher plant and ear heights. 1. When oats was seeded on August 20. the first heading and 50% heading dates were recorded on October 4 and 15, respectively. And when oats was seeded on August 25. the first heading and 5 0'!4 heading dates were observed on October 13 and 18. respectively. As seeding date was delayed for one day. the heading date was delayed for two days. 3. The dry mattcr percentage of oats seeded on August 20 was 20.1%, but that of oats seeded after August 10 was 15.1 to 16.7%. 4. According to the contents of CP. ADF and NDF, oats seeded on August 20 and 25 was evaluated to be the 3rd or 4th grade hay. and the other oats sceded after August 15 was recorded the 2nd or 3rd grade hay as proposed by AFGC. 5. There are no significant dry matter yield differences among the seeding dates of oats such as August 20, 25 and 30. but a significant yield difference was recorded between oats seeded in August and September. The same trend as the dry matter yield was observed in in vitro digestible dry matter and crude protein yields of oats seeded in August and September. According to the results. early seeding of early matuaring oats may be a better option than late seeding after August 30 for silage or hay production following silage corn harvest in the middle northern area of Korea.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.4
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• pp.203-210
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• 2001

To establish the recycling system of animal manure(AM) for environmental preservation and improve the utilization of AM, this study was to investigate the effects of the types and nitrogen application rate of AM on herbage productivity, efficiency of nitrogen utilization, nutritive value and an increase of soil fertility and in mixed grassland. This sudy was arranged in split plot design. Main plots were the types of AM(Cattle feedlot manure, CFM; Pig manure fermented with sawdust, PMFS; cattle sluny, CS) and subplots were the application rate of animal manure, such as 100, 200 and 300kgNiha. I. DM yields of herbage were the highest with CS and decreased by application over ZOOkgNiha AM. 2. Crude protein(CP) ontent was the highest with CFM and followed by CS, and the lowest with PMFS, and increased as application rate of AM increased. 3. Nitrogen(N) yields of CS treatment was higher than that of CFM and CS. and increased significantly as application rate of AM increased(P<0.05). 4. The contents of NDF, ADF and TDN was hardly influenced by the types and application rate of AM. 5. Organic matter(0M) content in the soil was the highest with PMFS and followed by CFM and the lowest with CS. OM content increased significantly as application rate of AM increased(P<0.05). 6. Total nitrogen content of the soil was not affected by the type of AM, but increased significantly as application rate of AM increased(P<0.05). (Key words : Animal manure, Grassland, Cattle feedlot manure, Pig manure fermented with sawdust, Cattle slurry, Soil fertility)

• KSBB Journal
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• v.22 no.4
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• pp.228-233
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• 2007

The high cost and lack of vegetable oil are limiting the expansion of biodiesel production. The purpose of research was to investigate the potential of animal fats as biodiesel feedstock. In this paper, transesterification using alkali catalyst and methanol was performed to reaction, we carried out experiments that it was changed variables as reaction temperature, methanol molar ratio, catalyst types, amount of catalyst and reaction time. The optimum reaction condition for biodiesel production was reaction temperature 65$^{\circ}C$, potassium hydroxide 1.0% (w/w), oil to methanol molar ratio 1:15 and reaction time 20 min. In this reaction condition, the contents of fatty acid methyl ester was reached to about 98.7%. Also, properties of biodiesel were measured to correspond to domestic quality standard of acid values, density and viscosity.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.10C
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• pp.1001-1009
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• 2009

In this paper, an efficient blind frequency offset estimation method for radio resource saving in orthogonal frequency division multiplexing (OFDM) systems is proposed. In the proposed method, we obtain two time different received OFDM signal blocks by using the cyclic prefix and define the cost function by using the two OFDM signal blocks. We show that the cost function can be approximately expressed as a closed form cosine function. The approximated cosine function can be obtained from three independent cost function values calculated at three different frequency offsets. In the proposed method, the frequency offset can be estimated by calculating a frequency offset minimizing the approximated cosine function without searching all the frequency offset range. Unlike the conventional methods such as MUSIC method, the accuracy of the proposed method is independent of the searching resolution since the closed form solution exists. The computer simulation shows that the performance of the proposed method is superior to those of the MUSIC and the oversampling method.