• Nuclear Engineering and Technology
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• 제33권3호
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• pp.283-297
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• 2001

The CHF in PWR rod bundles is usually predicted by the local flow correlation approach based on subchannel analysis while difficulty exists due to the existence of spacer grids especially with mixing vanes. In order to evaluate the effect of spacer grids on CHF, the experimental rod bundle data with various types of spacer grids were analyzed using the subchannel code, COBRA-IV-i. For the Plain grid data, a CHF correlation was described as a function of local flow conditions and heated length, and then the residuals of the CHF in mixing vaned grids predicted by the correlation were examined in various kinds of grids. In order to compensate for the residual, three parameters, distances between grids and from the last grids to the CHF site, and equivalent hydraulic diameter were introduced into a grid parameter function representing the remaining effect of spacer grids predicted most of the CHF data points in plaing grids within $\pm$20 percent error band. Good agreement with the CHF data was also shown when the grid parameter function for mixing vaned grids of a specific design was used to compensate for the residuals of the CHF data predicted by the correlation.

• BMB Reports
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• 제37권3호
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• pp.330-338
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• 2004

Different types of cardiotoxin (I-V and n) were isolated and purified from the venom of the Taiwan cobra (Naja naja atra). The effects of these cardiotoxins were studied on membrane-bound acetylcholinesterase, which was isolated from a sheep's brain cortex. The results showed that cardiotoxins I-III, V, and n activated the enzyme by modification of substrate inhibition, but cardiotoxin IV's reaction was different. The inhibition and activation of acetylcholinesterase were linked to the functions of the hydrophobicity index, presence of a cationic cluster, and the accessible arginine residue. Our results indicate that Cardiotoxins have neither a cationic cluster nor an arginine residue in their surface area of loop I; therefore, in contrast to fasciculin, cardiotoxins are attached by loop II to the peripheral site of the enzyme. As a result, fasciculin seems to stabilize nonfunctional conformation, but cardiotoxins seem to stabilize the functional conformation of the enzyme. Based on our experimental and theoretical findings, similar secondary and tertiary structures of cardiotoxins and fasciculin seem to have an opposite function once they interact with acetylcholinesterase.