• 대한가정학회지
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• 제31권4호
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• pp.133-140
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• 1993

This study presents the rationale for the development of the Comparison Level Scale(CLS) and the Comparison Level for Alternatives Scale(CLAS). Data from 130 married women who live in the U.S.A. suggest that the CLS and the CLAS are internally consistent and unidimensional. The CLS and the CLAS are based on exchange theory perspectives, particularly, Thibaut and Kelley's theory, on evaluating an individual's comparative processes. The scales provide explanatory as well as descriptive power concerning evaluating an intimate relationship.

• 한국산학기술학회논문지
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• 제19권3호
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• pp.325-336
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• 2018

본 연구의 목적은 간호사를 대상으로 한 경력개발 관련 국내 연구의 최신 동향을 탐색하여 향후 간호 보건 분야에서 경력관련 논문의 이론적 접근과 실천적 방향성을 제시하고자 시행하였다. 연구 대상은 2008년부터 2017년까지 10년간 국내 학술지에 실린 간호사 경력개발 관련 연구였다. 검색 데이터베이스 RISS 및 KISS등을 통해 검색어를 '간호', '간호사', '경력', '경력개발', '경력개발 프로그램', '경력관리'로 하여 검색된 논문 중 최종 29편의 논문을 분석대상으로 선정하였다. 분석은 전체적인 동향분석을 위해 연도별, 출처별, 연구설계 및 대상자 별로 살펴보았고, 주제 별 경향성을 파악하기 위해서는 내용분석방법을 채택하였다. 내용분석 결과 상위 범주로 경력개발과 경력개발결과로 나뉘었고, 경력개발의 하위범주로 조직 경력관리, 개인경력관리 경력개발결과의 하위범주로 경력성과로 나뉘었다. 하위범주인 개인경력관리는 경력탐색, 경력계획, 경력정체, 경력단절로 구성되어 있었고, 조직경력관리는 CLS개발 및 적용, CLS 인식도 조사, 경력개발 모형구축으로 구성되어 있었다. 경력성과는 경력몰입, 조직몰입, 이직의도, CLS 적용 후 변화, 경력만족, 경력변경의도 순으로 구성되어 있었다. 그리고 가장 많이 다룬 주제는 경력성과 중 경력몰입으로 나타났다. 따라서 앞으로 경력개발 관련 간호연구의 방향성은 개인 차원과 조직차원의 욕구를 부합시킨 통합적인 연구가 필요하며, 또한 대상자, 연구설계와 연구주제에 있어서의 다양성이 요구된다.

• 한국전자통신학회논문지
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• 제3권1호
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• pp.1-7
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• 2008

해저광케이블은 국제통신의 98% 이상을 처리하고 있는 국가의 중요한 정보통신 인프라이다. 하지만 선박의 닻, 어선의 어구, 해저 지진 등 다양한 요인으로 인해 고장이 발생하며, 고장 발생시 대용량 해저통신 트래픽의 영향을 최소화하고, 케이블 수리 선박의 운영비용 절감을 위해서 수리 기간을 최대한 단축해야 한다. 해저광케이블 고장 유형은 Shunt Fault, Cable Cut, Open Fault, Fiber Break 등 여러 유형이 있으며, 고장이 발생하면 육상 터미널 국사(육양국)에서 정확하고 신속하게 고장 유형 및 고장 지점을 파악하고, 케이블 수리 선박은 수리 현장으로 출동하여 수리 작업을 수행하게 된다. 수리작업 기간 중 육양국에서는 케이블 수리 선박에서 요청하는 각종 Test를 수행하게 되며, 이때 육양국 Test 기법은 매우 중요하다. 본 논문에서는 해저광케이블 고장 유형 및 고장 지점 탐지 기법을 제시하고, 해저광케이블 수리기간 단축을 위한 육양국 Test 기법을 고찰하고자 한다.

• International Journal of Oral Biology
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• 제38권4호
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• pp.161-167
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• 2013

Human dental pulp stem cells (DPSCs) are multi-potent mesenchymal stem cells that have several differentiation potentials. An understanding of thetissues that differentiate from these cells can provide insights for future regenerative therapeutics and tissue engineering strategies. The mesiodens is the most frequent form of supernumerary tooth from which DPSCs can differentiate into several lineages similar to cells from normal deciduous teeth. Recently, it has been shown that nanoscale structures can affect stem cell differentiation. In our presentstudy, we investigated the effects of a 250-nm nanoscale ridge/groove pattern array on the osteogenic and adipogenic differentiation of dental pulp cells from mesiodenscontaining human DPSCs. To this end, the expression of lineage specific markers after differentiation induction was analyzed by lineage specific staining and RT-PCR. The nanoscale pattern arrayed surface showed apositive effect on the adipogenic differentiation of DPSCs. There was no difference between nanoscale pattern arrayed surface and conventional surface groups onosteogenic differentiation. In conclusion, the nanoscale ridge/groove pattern arrayed surface can be used to enhance the adipogenic differentiation of DPSCs derived from mesiodens. This finding provides an improved understanding of the effects of topography on cell differentiation as well as the potential use of supernumerary tooth in regenerative dental medicine.

• 한국수정란이식학회지
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• 제23권4호
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• pp.245-250
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• 2008

This study was designed to investigate the effect of essential amino acids (EAA) and/or non-essential amino acids (NEAA) on the development of parthenogenetic and somatic cell nuclear transfer (SCNT) porcine embryos in vitro. To evaluate the timing of amino acids supplementation, activated oocytes were cultured in NCSU23-PVA with EAA, NEAA or NEAA+EAA (AAs) during specific periods as below: EAA, NEAA or AAs were supplemented during Day 0 to 6 (whole culture period: ALL), Day 2 to Day 6 (post-maternal embryonic transition period: POST-MET), Day 5 to Day 6 (post-compaction period: POST-CMP), Day 0 to Day 2 (pre-maternal embryonic transition period: PRE-MET), or Day 0 to Day 4 (post-compaction period: PRE-CMP). Supplementation of NEAA decreased cleavage rates in PRE-MET and PRE-CMP and also decreased blastocyst rates in POST-CMP. On the other hand, EAA significantly enhanced blastocyst formation rate in POST-MET and no detrimental effect on embryonic development in other groups. Interestingly, NEAA and EAA had synergistic effect when they were supplemented to the medium during whole culture period. Supplementation of AAs also enhanced SCNT porcine embryo development whereas BSA-free medium without AAs could not supported blastocyst formation of SCNT embryos. In conclusion, existence of EAA and NEAA in defined culture medium variously influences the development of parthenogenetic and SCNT porcine embryos, and their positive effect are only occurred when both EAA and NEAA are supplemented to the medium during whole culture period. Additionally, AAs supplementation enhances the blastocyst formation of SCNT porcine embryos when they are cultured in the defined condition.

• 생물환경조절학회지
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• 제13권4호
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• pp.240-244
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• 2004

순환식 양액재배시 계면활성제가 무기이온 흡수 증진에 미치는 영향을 알아보고자 상추의 생육, 광합성율, 증산율 및 무기이온 함량을 분석하였다. 상추 생육은 $0.3mg{\cdot}L^{-1}$ PVA-95처리에서 가장 높았으며, l/2배액에서 재배된 상추의 생육도 양호하였다. 칼슘 함량이 증가된 $0.3mg{\cdot}L^{-1}$ CLS 처리의 상추는 생육이 낮음에도 불구하고 높은 증산율과 광합성율 및 엽내 카리, 칼슘 및 마그네슘 함량이 높았다. 따라서 높은 생육량과 비료 절감 효과가 인정된 PVA-95 처리와 CLS 처리에 의한 엽내 무기 성분 함량 증가가 인정됨에 따라 수경재배 상추의 고품질 생산에 유용하게 사용 가능하리라고 판단된다.

• 한국수정란이식학회지
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• 제26권2호
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• pp.111-115
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• 2011

In general, zona pellucida (ZP) of the blastocyst has to be removed first, then either isolated the inner cell mass (ICM) or ZP-removed whole blastocyst, which is then cultured on the feeder layer to induce ICM outgrowth for the generation of embryonic stem cells (ESC). However, it is unclear whether ICM isolation before seeding on feeder layer is beneficial or not because the interaction between ICM and trophoblasts may affect cellular growth and/or pluripotency during the culture on the feeder. In the present study, two ZP removal methods (mechanically by splitting with a 28-gauge needle versus chemically by the treatment of acid-Tyrode's solution) and two ICM isolation methods (ZP-free whole blastocyst seeding versus mechanical isolation of ICM) were evaluated for the efficient isolation and culture of putative parthenogenetic bovine ESC. The number of maintained outgrown colonies was counted in each experimental group. As the result, mechanical removal of ZP with a needle and followed by whole ZP-free blastocyst seeding on feeder cells tended to attach more on the feeder layer and resulted in more outgrown colonies with its simple and less time-costing benefits. Currently we are generating ESC lines in HanWoo cattle by using this method for initial outgrowth of the parthenogenetic bovine blastocysts.

• International Journal of Oral Biology
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• 제35권1호
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• pp.1-5
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• 2010

Human fibroblasts that maintain the structural integrity of connective tissues by secreting precursors of the extracellular matrix are typically cultured with serum. However, there are potential disadvantages of the use of serum including unnatural interactions between the cells and the potential for exposure to animal pathogens. To prevent the possible influence of serum on fibroblast cultures, we devised a serum-free growth method and present in vitro data that demonstrate its suitability for growing porcine fetal fibroblasts. These cells were grown under four different culture conditions: no serum (negative control), 10% fetal bovine serum (FBS, positive control), 10% knockout serum replacement (KSR) and 20% KSR in the medium. The proliferation rates and viabilities of the cells were investigated by counting the number of cells and trypan blue staining, respectively. The 10% FBS group showed the largest increase in the total number of cells ($1.09\;{\times}\;10^5\;cells/ml$). In terms of the rate of viable cells, the results from the KSR supplementation groups (20% KSR:64.7%; 10% KSR: 80.6%) were similar to those from the 10% FBS group (68.5%). Moreover, supplementation with either 10% ($3.0\;{\times}\;10^4\;cells/ml$) or 20% KSR ($4.8\;{\times}\;10^4\;cells/ml$) produced similar cell growth rates. In conclusion, although KSR supplementation produces a lower cell proliferation rate than FBS, this growth condition is more effective for obtaining an appropriate number of viable porcine fetal fibroblasts in culture. Using KSR in fibroblast culture medium is thus a viable alternative to FBS.

• 한국수정란이식학회지
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• 제25권2호
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• pp.97-101
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• 2010

Intracytoplasmic sperm injection (ICSI) is one of the artificial fertilization methods when only a few sperm are available for insemination, and an important tool for the preservation of genetic materials of endangered animal species, especially the male is infertile. Different from other species such as mice and pigs, the conventional ICSI method which uses spiked pipette for injection (Spike-ICSI) is exhibited low success rates in cattle because the bovinesperm head membrane is hard to break during injection procedure. We chose piezo-assisted ICSI (Piezo-ICSI) for the improvement of the injection procedure including sperm head membrane rupture and efficient puncture of the plasma membrane of the oocytes. In this experiment, we compared the efficacy of the bovine ICSI embryo production between the Piezo-ICSI and Spike-ICSI. The second polar body extrusion, pronuclear formation, cleavage and blastocyst formation were evaluated after implementation of two different ICSI techniques. The Piezo-ICSI tended to show comparably higher rates of the second polar body extrusion (41.7%), the pronuclei formation (42.9%) and the two-cell cleavage (41.4%) than Spike-ICSI does (33.3%, 28.6% and 23.5%, respectively) although there is no statistic significance between two groups. In addition, the blastocysts were only obtained from the Piezo-ICSI group (10.3%). Our finding shows that the Piezo-ICSI may be used as an artificial fertilization method in cattle when in vitro fertilization is not applicable.

• 임상간호연구
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• 제24권3호
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• pp.324-335
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• 2018

Purpose: The purpose of this study was to propose a revised Clinical Ladder System(CLS) Model for nurses based on the evaluation of clinical competence and professional activities of nurses working in general hospitals. Methods: Data were collected between September 10 and October 30, 2017. Participants were 50 head nurses from 10 general hospitals with over 400 beds located in Seoul City and Gyeonggi Province. Each head nurse evaluated clinical competence, qualifications, and professional activities of 5 staff nurses at each of the 5 levels of CLS in her unit. The total number of the nurses evaluated was 245. Data were analyzed with descriptive statistics and t-test, one-way ANOVA, and $Scheff{\acute{e}}$. Results: Over 80% of the nurses were university graduates. As the CLS levels increased, clinical competence, qualifications, and professional activities also increased significantly. Education material development and quality improvement activities were carried out by nurses from level 2, research and evidence based practice activities were carried out from level 3, and nurses at level 4 or 5 participated in most of the professional activities as leaders. Conclusion: In order to retain excellent nurses in general hospitals, recognizing and rewarding nurses according to the revised model of the CLS are recommended.