• Title/Summary/Keyword: CLS

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In Vitro Development of Porcine Parthenogenetic Embryos under the Oil-free Culture System

  • Park, Sang-Kyu;Choi, Young-Ju;Roh, Sang-Ho
    • Journal of Embryo Transfer
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    • v.25 no.4
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    • pp.259-262
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    • 2010
  • Optimization of the preimplantation mammalian embryo culture condition was widely focused on refining medium composition under the name of chemically defined media. However, recent research revealed that the alteration of physical environment can be a crucial factor to a successful embryo development. In this study, under the same embryo density, a novel culture device named oil-free micro tube culture (MTC) system was evaluated using porcine parthenogenetic embryos. The activated oocytes were placed into the 0.2 ml thin-wall flat cap PCR tube and cultured to the blastocyst stage. As a preliminary step, embryo density and culture medium volume were optimized under a standard drop culture system. The optimal embryo density range for in vitro culture was 0.5 embryos per ${\mu}l$ in $20\;{\mu}l$ drop (20.5%) and 1.0 embryos per ${\mu}l$ in $10\;{\mu}l$ drop (20.6%). Based on these results, we compared drop culture system and 'MTC' system in terms of the developmental rate to the blastocyst stage. In $20\;{\mu}l$ medium volume, the 'MTC' system showed similar blastocyst formation rate when compared with drop culture system (20.2% versus 20.5%, respectively) while the 'MTC' system showed lower blastocyst formation rate than drop culture system in $10\;{\mu}l$ one (12.7% versus 20.0%, respectively). Therefore the $20\;{\mu}l$ MTC system may be an alternative incubation system for short-distance embryo transport without carrying the $CO_2$ incubator and this provides novel embryo culture device to clinical veterinary embryologists.

A Novel Carrier Leakage Suppression Scheme for UHF RFID Reader (UHF 대역 RFID 리더 반송파 누설 억압 연구)

  • Jung, Jae-Young;Park, Chan-Won;Yeom, Kyung-Whan
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.22 no.4
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    • pp.489-499
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    • 2011
  • RFID technologies, which allow collecting, storing, processing, and tracking information by wirelessly recognizing the inherent ID of object through an attached electronic tag, have a variety of application areas. This paper presents a novel carrier leakage suppression RF(CLS-RF) front-end for ultra-high-frequency RF identification reader. The proposed reader CLS-RF front-end structure generates the carrier leakage replica through the nonlinear path that contains limiter. The limiting function only preserves the frequency and phase information of the leakage signal and rejects the amplitude modulated tag signal in the envelope. The carrier leakage replica is then injected into the linear path that contains phase shifter. Therefore, the carrier leakage signal is effectively cancelled out, while not affecting the gain of the desired tag backscattering signal. We experimentally confirm that the prototype shows a significant improvement in the leakage to signal ratio by up to 36 dB in 910 MHz, which is consistent with our simulation results.

A Circle Labeling Scheme without Re-labeling for Dynamically Updatable XML Data (동적으로 갱신가능한 XML 데이터에서 레이블 재작성하지 않는 원형 레이블링 방법)

  • Kim, Jin-Young;Park, Seog
    • Journal of KIISE:Databases
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    • v.36 no.2
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    • pp.150-167
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    • 2009
  • XML has become the new standard for storing, exchanging, and publishing of data over both the internet and the ubiquitous data stream environment. As demand for efficiency in handling XML document grows, labeling scheme has become an important topic in data storage. Recently proposed labeling schemes reflect the dynamic XML environment, which itself provides motivation for the discovery of an efficient labeling scheme. However, previous proposed labeling schemes have several problems: 1) An insertion of a new node into the XML document triggers re-labeling of pre-existing nodes. 2) They need larger memory space to store total label. etc. In this paper, we introduce a new labeling scheme called a Circle Labeling Scheme. In CLS, XML documents are represented in a circular form, and efficient storage of labels is supported by the use of concepts Rotation Number and Parent Circle/Child Circle. The concept of Radius is applied to support inclusion of new nodes at arbitrary positions in the tree. This eliminates the need for re-labeling existing nodes and the need to increase label length, and mitigates conflict with existing labels. A detailed experimental study demonstrates efficiency of CLS.

Induction of superovulation in mature mice and rats using serum of spayed female dogs

  • Nooranizadeh, MH;Mogheiseh, A;Kafi, M;Sepehrimanesh, M;Vaseghi, H
    • Laboraroty Animal Research
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    • v.34 no.4
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    • pp.211-215
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    • 2018
  • The following experiments were designed to examine the effect of serum of spayed dogs on superovulation response in mice and rats. In Experiment 1, female mice at diestrus (n=30) were divided into three equal groups and superovulated with either administration of 5 IU pregnant mare serum gonadotropin (PMSG) or recombinant follicle stimulating hormone (rFSH) (reducing dose from 2.5 to 0.5 IU) and 5 IU human chorionic gonadotropin (hCG) administered 48h later. Serum of spayed dogs was administered intraperitoneally at a reduced dose from 0.1 to 0.025 mL in a 48 h period. In Experiment 2, female rats (n=30) at diestrus stage were divided into three equal groups. Superovulation was induced using either 30 IU PMSG, or a dose reduced from 5 to 1 IU rFSH and 25 IU hCG administered 48h later. Serum of spayed dogs was administered in a reduced dose from 0.6 to 0.1 mL in a 48 hour period. Female mice and rats were mated 24 h following hCG administration. On day 14 after mating, animals were euthanized and ovarian sections were fixed for histopathological evaluation and corpus luteum (CL) counting. No significant difference observed in mean (${\pm}SEM$) number of CLs between the PMSG group and the mice that received serum of spayed dog ($10.4{\pm}1.3$ vs $9.2{\pm}1.0$). Mean (${\pm}SEM$) number of CLs tended to be lower in rats that received serum of spayed dog than those of rats which received either PMSG or rFSH ($15.1{\pm}1.9$ vs $23.6{\pm}3.1$ and $23.1{\pm}2.9$, P=0.06, respectively). In conclusion, serum of spayed dogs is able to induce a superovulatory response in mice and rats.

Document Classification Methodology Using Autoencoder-based Keywords Embedding

  • Seobin Yoon;Namgyu Kim
    • Journal of the Korea Society of Computer and Information
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    • v.28 no.9
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    • pp.35-46
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    • 2023
  • In this study, we propose a Dual Approach methodology to enhance the accuracy of document classifiers by utilizing both contextual and keyword information. Firstly, contextual information is extracted using Google's BERT, a pre-trained language model known for its outstanding performance in various natural language understanding tasks. Specifically, we employ KoBERT, a pre-trained model on the Korean corpus, to extract contextual information in the form of the CLS token. Secondly, keyword information is generated for each document by encoding the set of keywords into a single vector using an Autoencoder. We applied the proposed approach to 40,130 documents related to healthcare and medicine from the National R&D Projects database of the National Science and Technology Information Service (NTIS). The experimental results demonstrate that the proposed methodology outperforms existing methods that rely solely on document or word information in terms of accuracy for document classification.

Relationship between Low-level Clouds and Large-scale Environmental Conditions around the Globe

  • Sungsu Park;Chanwoo Song;Daeok Youn
    • Journal of the Korean earth science society
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    • v.43 no.6
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    • pp.712-736
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    • 2022
  • To understand the characteristics of low-level clouds (CLs), environmental variables are composited on each CL using individual surface observations and six-hourly upper-air meteorologies around the globe. Individual CLs has its own distinct environmental conditions. Over the eastern subtropical and western North Pacific Ocean in JJA, stratocumulus (CL5) has a colder sea surface temperature (SST), stronger and lower inversion, and more low-level cloud amount (LCA) than the climatology whereas cumulus (CL12) has the opposite characteristics. Over the eastern subtropical Pacific, CL5 and CL12 are influenced by cold and warm advection within the PBL, respectively but have similar cold advection over the western North Pacific. This indicates that the fundamental physical process distinguishing CL5 and CL12 is not the horizontal temperature advection but the interaction with the underlying sea surface, i.e., the deepening-decoupling of PBL and the positive feedback between shortwave radiation and SST. Over the western North Pacific during JJA, sky-obscuring fog (CL11), no low-level cloud (CL0), and fair weather stratus (CL6) are associated with anomalous warm advection, surface-based inversion, mean upward flow, and moist mid-troposphere with the strongest anomalies for CL11 followed by CL0. Over the western North Pacific during DJF, bad weather stratus (CL7) occurs in the warm front of the extratropical cyclone with anomalous upward flow while cumulonimbus (CL39) occurs on the rear side of the cold front with anomalous downward flow. Over the tropical oceans, CL7 has strong positive (negative) anomalies of temperature in the upper troposphere (PBL), relative humidity, and surface wind speed in association with the mesoscale convective system while CL12 has the opposite anomalies and CL39 is in between.

Effect of Liquid Manure Source, Application Rate and Time on Agronomic Characteristics and Forage Yield of Winter Rye (가축분뇨의 종류, 시비량 및 시비시기가 호밀의 생육특성과 생산성에 미치는 영향)

  • Park, Jin-Gil;Kim, Jong-Duk;Kwon, Chan-Ho
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.26 no.4
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    • pp.227-232
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    • 2006
  • This experiment was conducted to evaluate the effect of liquid manure source, application rate and time on the agronomic characteristics and forage yield of winter rye. The experimental design was a randomized complete block design. The treatments were : CFB150=chemical fertilizer(CF) 150 N as basal, CFS150=CF 150 N as split application (75+75), SLB150=swine liquid (SL) 150 N as basal, SLS150=SL 150 N as split application (75+75), SLB300=SL 300 N as basal, SLS150=SL 300 N as split application(150+150), CLB150=cattle liquid (CL) 150 N as basal, CLS150=CL 150 N as split awlication(75+75), CLB300=CL 300 N as basal, CLS150=CL 300 N as split application(150+150). Heading date of the plant was observed on the 17th of April fur both chemical fertilizer and swine liquid, and on the 16th of April for cattle liquid. Stay green of chemical fertilizer was higher than others because of high crude protein content. Leaf was darker in high nitrogen fertilizer treatments than low N treatments. However lodging resistance was poor as nitrogen fertilizer was increased. Dry matter (DM) content of rye at chemical fertilizer was lower than liquid manure. DM yield of chemical fertilizer treatments were highest among the fertilizer source. However, DM yield of rye with application was all most same at different N application methods. The crude protein (CP) content and yield for chemical fertilizer was significantly higher than liquid manure. CP yield using split application was higher by 16% and 28%, compared to basal application. Based on the results of this study, forage production of liquid manure was lower, compared to chemical fertilizer. And split application was superior to basal application forage and protein yields, and high protein.

To Assess Whether Lee's Grading System for Central Lumbar Spinal Stenosis Can Be Used as a Decision-Making Tool for Surgical Treatment (요추 중심 신경관 협착에 있어서 Lee's Grade를 통한 MRI 평가방법이 수술적 치료 결정에 유용한가에 대한 연구)

  • Do Yeon Ahn;Hee Jin Park;Jung Woo Yi;Ji Na Kim
    • Journal of the Korean Society of Radiology
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    • v.83 no.1
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    • pp.102-111
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    • 2022
  • Purpose To evaluate the correlation between Lee's grades and surgical intervention for central lumbar spinal stenosis (CLSS) and to assess whether this grading system can be used as a decision-making tool for the surgical treatment of this condition. Materials and Methods This retrospective study included 290 patients (M:F = 156:134; mean age, 46 ± 16 years). Radiologists assessed the presence and grade of CLSS at the stenosis point according to Lee's grading system, in which CLSS is classified into four grades according to the shape of the cauda equina. Correlation coefficients (rs) between Lee's grades and the operation were calculated with Spearman rank correlation. Results Among the operated patients, grade 2 was the most commonly assigned grade (50%-58%), grade 3 was less common (35%), and grade 0 was the least common (2%-3%). Among the non-operated patients, grade 1 was the most common (63%-65%), grade 0 was less common (15%-16%), and grade 3 was the least common (8%). The distribution of grades differed between the operated and non-operated groups (p < 0.001). Less than 25% of patients who underwent surgery were assigned grades 0 and 1, and more than 88% were assigned grades 2 and 3. A moderate correlation was found between the grade and surgical intervention (rs = 0.632 and rs = 0.583). Conclusion Lee's grade was moderately correlated with surgical intervention. Lee's grading system can be a decision-making tool for the surgical treatment of CLSS.

Comparison of Offshore Wind Retrieval Software from SAR Satellite Imagery (SAR 위성영상 해상풍 추출 소프트웨어 비교)

  • Kim, Hyun-Goo;Hwang, Hyo-Jung;Kang, Yong-Heack;Yun, Chang-Yeol
    • New & Renewable Energy
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    • v.9 no.3
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    • pp.14-19
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    • 2013
  • Comparative evaluation of offshore wind retreival software, which use the satellite images taken by Synthetic Aperture Radar sensor; SARTools of CLS-SOPRONO, France and SpaceEye of London Research and Development Corporation, Canada is carried out. For a reference satellite image, ENVISAT ASAR imagery of Jeollanam-do Wan-do area when the winter-time northwestern wind prevails is processed by CMOD_IFR2, CMOD4, CMOD5 algorithms. Wind speed difference and its relative ratio are calculated to evaluate uncertainty of software selection.

Variable-node-flat shell element for adaptive mesh refinement (적응적체눈세분화를 위한 변절점 평면 쉘 요소)

  • 최창근;이완훈
    • Proceedings of the Computational Structural Engineering Institute Conference
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    • 1994.10a
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    • pp.1-8
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    • 1994
  • A variable-node-flat shell element designated as CLS which has variable mid-side nodes with drilling freedom has been presented in this paper. The shell element to be applied in finite element analysis has been developed by combining a membrane element named as CLM with drilling rotation d.o.f. and plate bending element. The combined shell element possess six degrees of freedom per node. By introducing the variable-node elements which have physical midside nodes, some difficulties associated with imposing displacement constraints on irregular nodes to enforce interelement compatibility in common adaptive h-refinement on quadrilateral mesh are easily overcome. Detailed numerical studies show the excellent performance of the new shell elements developed in this study.

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