• Journal of Life Science
• /
• v.32 no.11
• /
• pp.833-840
• /
• 2022

Chronic inflammation has been shown to be closely associated with tumor development and progression. Nuclear factor kappa B (NF-κB) is composed of a family of five transcription factors. NF-κB signaling plays a crucial role in the inflammatory response and is often found to be dysregulated in various types of cancer, making it an attractive target in cancer therapeutics. In this study, CDC-like kinase 3 (CLK3) was identified as a novel kinase that regulates the NF-κB signaling pathway. Our data demonstrate that CLK3 inhibits the canonical and non-canonical NF-κB pathways. Luciferase assays following the transient or stable expression of CLK3 indicated that this kinase inhibited NF-κB activation mediated by Tumor necrosis factor-alpha (TNFα) and Phorbol 12-myristate 13-acetate (PMA), which are known to activate NF-κB signaling via the canonical pathway. Consistent with data on the ectopic expression of CLK3, CLK3 knockdown using shRNA constructs increased NF-κB activity 1.5-fold upon stimulation with TNFα in HEK293 cells compared with the control cells. Additionally, overexpression of CLK3 suppressed the activation of this signaling pathway induced by NF-κB-inducing kinase (NIK) or CD40, which are well-established activators of the non-canonical pathway. To further examine the negative impact of CLK3 on NF-κB signaling, we performed Western blotting following the TNFα treatment to directly identify the molecular components of the NF-κB pathway that are affected by this kinase. Our results revealed that CLK3 mitigated the phosphorylation/activation of transforming growth factor-α-activated kinase 1 (TAK1), inhibitor of NF-κB kinase alpha/beta (IKKα/α), NF-κB p65 (RelA), NF-κB inhibitor alpha (IκBα), and Extracellular signal-regulated kinase 1/2-Mitogen-activated protein kinase (ERK1/2-MAPK), suggesting that CLK3 inhibits both the NF-κB and MAPK signaling activated by TNFα exposure. Further studies are required to elucidate the mechanism by which CLK3 inhibits the canonical and non-canonical NF-κB pathways. Collectively, these findings reveal CLK3 as a novel negative regulator of NF-κB signaling.

• Journal of the Institute of Electronics Engineers of Korea SD
• /
• v.39 no.4
• /
• pp.72-83
• /
• 2002

In this paper, a novel low-power VLSI system architecture was proposed. By exploiting software control of supply voltages, it simplifies hardware implementation, reduces power consumption efficiently, and avoids complicated interface circuits. The proposed architecture models clock frequency-supply voltage relationship by software modelling, enables individual control of supply voltages for all chips in the system, and restricts clock frequency to discrete levels of $f_{CLK}$, $f_{CLK}$2, $f_{CLK}$3... where $f_{CLK}$ is the master clock frequency A prototype system was implemented by modifying off-the-shelf microprocessor evaluation board and adding simple discrete devices such as level shifters and voltage switches. It was measured that the power consumption was reduced from 0.58W to 0.12W in the Prototype system. system.

• ETRI Journal
• /
• v.26 no.6
• /
• pp.669-672
• /
• 2004

To improve the operation error caused by the thermal fluctuation of electrons, we propose a novel single-electron pass-transistor logic circuit employing a multiple-tunnel junction (MTJ) scheme and modulate a parameters of an MTJ single-electron tunneling device (SETD) such as the number of tunnel junctions, tunnel resistance, and voltage gain. The operation of a 3-MTJ inverter circuit is simulated at 15 K with parameters $C_g=C_T=C_{clk}=1\;aF,\;R_T=5\;M{\Omega},\;V_{clk}=40\;mV$, and $V_{in}=20\;mV$. Using the SETD/MOSFET hybrid circuit, the charge state output of the proposed MTJ-SETD logic is successfully translated to the voltage state logic.

• Proceedings of the IEEK Conference
• /
• 2006.06a
• /
• pp.671-672
• /
• 2006

Recently Communication with using IrDA is bing used in various fields. In this paper I designed a receiver by fabricating hardware that used to be fabricated through software, so anyone who don't have knowledge on IrDA can receive Ir Signal easily. This receiver can communicate with CPU through 8 bit data and 3 bit address. Also this receiver can use user-needed CLK because this receiver embodied 16 bit CLK Prescaler.

• Bulletin of the Korean Space Science Society
• /
• 2010.04a
• /
• pp.36.1-36.1
• /
• 2010

GNSS(Global Navigation Satellite System)의 하나인 GPS(Global Positioning System)를 이용한 정밀 측위에 있어서 위성의 시계오차는 측위 정확도에 매우 큰 영향을 미친다. GPS위성에는 세슘(Cs)과 루비듐(Rb)으로 이루어진 4개의 원자시계가 탑재되어있으며 현재 사용하고 있는 원자시계의 종류는 NANU(GPS Notice Advisory to Navster Users) 정보를 통해 알 수 있다. 이 연구에서는 IGS(International GNSS Service)에서 제공하는 sp3 파일과 clk 파일을 이용하여 위성시계 특성을 분석하였다. 2000년부터 2009년까지의 sp3 파일에서 각 PRN에 대한 위성시계오차 값을 추출하여 그래프로 분석하였다. 그 결과 대부분의 세슘시계는 직선형태, 루비듐시계는 곡선형태의 특성을 보였으나 일정한 경향은 나타나지 않음을 알 수 있었다. 또한 3주간의 clk 파일에서 위성시계오차 값을 추출하여 각 PRN별로 1차식과 2차식으로 접합(fitting)하고 그 결과를 비교하였다. 세슘시계의 위성시계오차 값의 경우 2차식보다 1차식이 추출 데이터와 일치함을 알 수 있었으며 세슘시계의 위성시계오차 값은 직선형태의 특성을 보이는 것을 확인 할 수 있었다. 그리고 Modified Allan Deviation(MADEV) 방법을 적용하여 분석한 결과 GPS 위성의 block 별로 서로 다른 특성이 보임을 확인할 수 있었다.

• Journal of the Institute of Electronics Engineers of Korea SD
• /
• v.48 no.10
• /
• pp.15-19
• /
• 2011

We have proposed a maximum power point tracking (MPPT) circuit for thermoelectric generator (TEG) using a Boost converter. The key point of the proposed MPPT circuit is that the duty ratio of the boost converter automatically moves to Maximum Power Point by comparing of consecutive sampling voltage using two comparators. From the simulation results, we showed that the proposed circuit can find the maximum power point within 2 CLK periods and to generate optimal PWM signal within 3 CLK periods. The proposed MPPT circuit was designed by using a CMOS 0.18 um process, and it is now on the fabrication.

• Clinical and Experimental Pediatrics
• /
• v.48 no.7
• /
• pp.779-788
• /
• 2005

Purpose : Echinacea, a traditional plant medicine has been used as immune-stimulant. Recent studies have revealed that extract of Echinacea has immunostimulatory effects on human blood mononuclear cells. This study was designed for the purpose of screening the genes associated with immunologic effects of Echinacea on monocytes and dendritic cells using a cDNA microarray chip. Methods : $CD14^+$ monocyte cells were cultured for one day with Echinacea extract(final concentration : $50{\mu}g/mL$) in experiment 1, but were cultured without Echinacea in experiment 2. The gene expression of these cultured monocytes was analyzed using the cDNA microarray chip. Dendritic cells produced from $CD14^+$ monocyte were cultured for five days with GM-CSF and IL-4, and then cultured for one day with Echinacea in experiment 3, but were done without Echinacea in experiment 4. Results : In experiments 1 and 2, there were 17 significantly expressed genes with average expression ratios above 2.5, including interferon gamma-inducible protein 30(IFI 30), CDC(cell-division-cylcle)-like kinase 2(CLK 2), syndecan binding protein(syntenin), superoxide dismutase 2, etc. In experiments 3 and 4, there were 24 gene, with significantly expressed genes were 24 genes, which were insulin-like growth factor 2(somatomedin A), methyl-CpG binding domain protein 3, IFI 30, small inducible cytokine subfamily A, member 22, etc. The genes encoding CD44, IFI 30, mannose receptor C type 1(MRC 1), chemokine receptor 7(CCR 7), CLK 2, syntenin and cytochrome C oxidase subunit VIII were significantly expressed in both monocytes and dendritic cells cultured with Echinacea. Conclusion : This study employed a cDNA microarray chip to elicit the immune-associated gene profile; the expression was enhanced by Echinacea in CD14+ monocytes and dendritic cells. Thus we laid the basis for the quantitative and functional analysis of genes induced by Echinacea in monocytes and monocyte-derived dendritic cells.

• Korean Journal of Poultry Science
• /
• v.50 no.4
• /
• pp.193-202
• /
• 2023

Influenza IAVs are encapsulated negative-strand RNA viruses that infect many bird species' respiratory systems and can spread to other animals, including humans. This work reanalyzed previous microarray datasets to identify common and specific differentially expressed genes (DEGs) in chickens, as well as their biological activities. There were 760 and 405 DEGs detected in HPAIV and LPAIV-infected chicken cells, respectively. HPAIV and LPAIV have 670 and 315 DEGs, respectively, with both viruses sharing 90 DEGs. Because of HPAIV infection, numerous genes were implicated in a fundamental biological function of the cell cycle, according to the functional annotation of DEGs. Of the targeted genes, expressions of CDC Like Kinase 3 (CLK3), Nucleic Acid Binding Protein 1 (NABP1), Interferon-Inducible Protein 6 (IFI6), PIN2 (TERF1) Interacting Telomerase Inhibitor 1 (PINX1), and Cellular Communication Network Factor 4 (WISP1) were altered in DF-1 cells treated with polyinosinic:polycytidylic acid (PIC), a toll-like receptor 3 (TLR3) ligand, suggesting that transcription of these genes be controlled by TLR3 signaling. To gain a better understanding of the pathophysiology of AIVs in chickens, it is crucial to focus more research on unraveling the mechanisms through which AIV infections may manipulate host responses during the infection process. Insights into these mechanisms could facilitate the development of novel therapeutic strategies.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
• /
• v.28 no.6
• /
• pp.563-571
• /
• 2010

The GPS satellite has three or four atomic clocks that consist of cesiums and rubidiums and the NANU messages can be used to identify the kind of the onboard atomic clock because they classify the clock type on a daily basis. In this study, for long-term analysis of the GPS satellite clock behavior, we extracted satellite clock errors for every PRN from years 2001 through 2009 using the SP3 files that are provided by the IGS. As a result, the cesium clock offsets usually have a linear trend of drifting. On the other hand, rubidium offsets show curvilinear variations in general, even though they cannot be represented as anyone specific polynomial function. For short-term analysis, we extracted satellite clock errors for each PRN for a week-long period using the CLK files that are also provided by the IGS and curve-fitted them with first-order and second-order polynomial functions. In cases of cesium clock errors, they were well-represented by first-order polynomial functions and rubidium clock errors were similar with second-order polynomials. However, some of rubidium clock errors could not be represented as any polynomial fitting function. To analyze the characteristic of GPS satellite by each block and atomic clock, we applied Modified Allan Deviation criterion to the dataset from years 2007 and 2010. We found that the Modified Allan Deviation characteristics changed significantly according the block and atomic clock type.

• Journal of the Institute of Electronics Engineers of Korea SD
• /
• v.42 no.2 s.332
• /
• pp.1-8
• /
• 2005

An area-efficient DC-DC voltage up-converter in a poly-Si TFT technology for system-on-glass is described which provides low-ripple output. The voltage up-converter is composed of charge-pumping circuit, comparator with threshold voltage mismatch compensation, oscillator, buffer, and delay circuit for multi-phase clock generation. The low ripple output is obtained by multi-phase clocking without increasing neither clock frequency nor filtering capacitor The measurement results have shown that the ripple on the output voltage with 4-phase clocking is 123mV, while Dickson and conventional cross-coupled charge pump has 590mV and 215mV voltage ripple, respectively, for $Rout=100k\Omega$, Cout-100pF, and fclk=1MHz. The filtering capacitor required for 50mV ripple voltage is 1029pF and 575pF for Dickson and conventional cross-coupled structure, for Iout=100uA, and fclk=1MHz, while the proposed multi-phase clocking DC-DC converter with 4-phase and 6-phase clocking requires only 290pF and 157pF, respectively. The efficiency of conventional and the multi-phase clocking DC-DC converter with 4-phase clocking is $65.7\%\;and\;65.3\%$, respectively, while Dickson charge pump has $59\%$ efficiency.