• Title/Summary/Keyword: CL response

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The Monohydrogen Arsenate-sensing Electrodes (Monohydrogen Arsenate 감응 전극)

  • Gwon-Shik Ihn;Il-Bae Park
    • Journal of the Korean Chemical Society
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    • v.31 no.2
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    • pp.162-167
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    • 1987
  • Four component $Ag_2S$-PbS-$PbHAsO_4-Cu_2S$ and three component $Ag_2S$-PbS-$PbHAsO_4$ electrodes have been prepared and evaluated for the direct measurement of monohydrogen arsenate. The 3.0 : 0.5 : 1.0 : 0.25 (mole ratio, $Ag_2S$:PbS:$PbHAsO_4:Cu_2S$) composition is superior in terms of potentiometric response, stability, rapidity of response and reproducibility. Testing was done over the concentration range $10^{-1}$~$10^{-4}M\;HA_SO_4^{2-}$in 0.1F NH4Ac-NH4OH buffer solution at pH 8.50 with constant ionic strength. Interfering ions were $CN^-,\;I^-,\;S^{-2}$ and $Cl^-$.

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Changes of serum cortisol concentration and stress responses in cohe salmon(Oncorhynchus kisutch) to netting (그물작업에 의한 은연어의 스트레스 반응)

  • JEON Joong-Kyun;KIM Pyong-Kih;PARK Yong-Joo;MYOUNG Jung-Goo;KIM Jong-Man
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.2
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    • pp.115-118
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    • 2000
  • The production of cultured coho salmon (Oncorhpchus kisutoh) in Korea has being increased year after year. Smolt being reared in freshwater suffer transferring into seawater and are farmed in cages for fattening. This handling processes including transportation, confinement into cages are unavoidable stress to fish in salmon farming and often end up to mass mortality, This study aimed to investigate the impact of handling process on the stress responses of coho salmon. The indicator of stress was measured by cortisol to be a first response, and for the second response test, glucose, triBlyceride, cholesterol, lactate and electrolyte of $K^+, Na^+, Cl^-$ in serum and the activities of alanine aminotrtnferase (ALT), aspartate aminotransferase(AST) and lactate dehydrogenase (LDH) were analyzed. As a result, the concentration of cortisol, glucose as well as LDH activity were significantly increased, whereas others showed no difference comparing with control group. It obviously demonstrated that handling process made fish stressful.

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Effects of Acute Change of Salinity on Physiological Response and Survival in Hybrid Striped Bass (Morone Chrysops male X Msaxatilis female) (빠른 염분변화가 Striped bass 잡종 (Morone Chrysops male X Msaxatilis female)의 생리적 반응과 생존에 미치는 영향)

  • LIM Han Kyu;LEE Jong Ha;JEONG Min Han;HAN Hyoung-Kyun;LIM Young Soo;LEE Jeong-Ho;LEE Byung-Chul
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.37 no.2
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    • pp.116-121
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    • 2004
  • Physiological responses and tolerance limit of hybrid striped bass $(Morone\;Chrysops\;male{\times}\;M.\;saxatilis\;female),$ were investigated on salinity changes from 33 psu to 0 psu and from 0 to psu 33 psu in two separate trials, respectively. In the acute salinity decrease from sea water (33 psu) to fresh water (0 psu), plasma level of cortisol, glucose, total protein, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were not affected, however, $Na^+,\;Cl^-$ and osmolality significantly decreased. In the acute salinity increase from fresh water to sea water (33 psu), plasma cortisol level did not change. Glucose and total protein concentrations in plasma and hematocrit (Hct) decreased, whereas AST, ALT, $Na^+,\;Cl^-$ and osmolality increased. The hybrid striped bass can overcome the acute changes of salinity, from 0 psu to 33 psu or from 33 psu to 0 psu, But salinity decrease stressed the fish much more than salinity increase.

Fabrication of $Cu_2/CdS$ solar cell and its characteristics ($Cu_2/CdS$ 태양전지 제작 및 그 특성연구)

  • 유평렬;김현숙;이재윤;강창훈;박은옥;정태수;김택성;양동익;신영진
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.7 no.2
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    • pp.315-323
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    • 1997
  • The sing1e crystal of cadmium sulfide was grown by vertical sublimation method. The lattice constants of CdS single crystal by extrapolation method are $a_0=4.139\AA$ and $c_0=6.719\AA$, respectively. The $Cu_2$S/CdS solar cell was fabricated using the single crystal of cadmium sulfide and the CuCl solution. The light- to- dark JV cross over effect of the $Cu_2$S/CdS solar cell was measured after annealing for 2 minutes at $250^{\circ}C$ in air atmosphere. The values of Voc, Jsc, Vop, FF, and efficiency are 0.40 volt, $4.2mA/\textrm{cm}^2$, 0.31 volt, $3.8mA/\textrm{cm}^2$, 0.68 and 3.8 %, respectively. The spectral response of the solar cell shows the peaks at 498 nm (2.49 eV) and 585 nm (2.12 eV).

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Serum Stress Responses during Seawater Acclimation in Coho Salmon, Oncorhynchus kisutch (은연어의 해수 순치에 따른 스트레스 반응의 변화)

  • Hong, Kyung-Pyo;Kim, Pyong-Kih;Jeon, Joong-Kyun;Kim, You-Hee;Park, Yong-Ju;Myoung, Jung-Goo;Kim, Jong-Man
    • Ocean and Polar Research
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    • v.26 no.3
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    • pp.433-438
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    • 2004
  • Stress response to the seawater acclimation in coho salmon (Oncorhynchus kisutch) smolt was investigated. Salt concentration of rearing water was gradually increased for 3 days from freshwater to seawater (30 ppt salt level). The changes of serum concentrations of cortisol as a primary stress indicator, and as secondary indicators, glucose (GLC), lactate (LAC), triglyce.ide (TG), cholesterol (CHOL), sodium ion $(Na^+)$, chloride ion $(Cl^-)$ and enzyme activities (alanine aminotransferase, ALT: aspartate aminotrasferase, AST; lactate dehydrogenase, LDH) were quantified during the acclimation experiment. Among them, cortisol, LAC, TG, CHOL, ALT, AST concentrations showed rapid increase at the first exposure to the 10ppt salt level (day 1), and began to decrease to the constant values after day 2 of adaptation at 20ppt salt level. However, LDH concentration tended to decrease during the whole experimental period. $Na^+\;and\;Cl^-$ showed slight decrease at day 1, and increased to a little bit higher values after day 2 rather than those in freshwater. All the fishes started on taking a food after day 4 of seawater adaptation. From these results, to reduce osmotic shock inducible stress to fish in seawater acclimation, gradual increase of salt levels is recommended.

Amperometric Enzyme Electrode for the Determination of $NH_4^+$ ($NH_4^+$ 정량을 위한 Amperometric Enzyme Electrode)

  • Moo Lyong Seo;Jae Sang Kim;Shim Sung Lee;Zun Ung Bae;Heung Lark Lee;Tae Myung Park
    • Journal of the Korean Chemical Society
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    • v.37 no.11
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    • pp.937-942
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    • 1993
  • Enzyme electrodes for amperometric measurement of ammonia was prepared by immobilization of L-glutamate dehydrogenase on an Immobilon-AV Affinity membrane and attachment to a glassy carbon electrode. Reduced nicotinamide adenine dinucleotide (NADH) was used as the electroactive species. The electrochemical oxidation of NADH was monitored at +1.0 volt vs. Ag/AgCl. Response was linear from $4.0\;{\times}\;10^{-5}\;to\;4.0\;{\times}\;10^{-4}$ M. The detection limit was 2.0 ${\times}\;10^{-6}$ M. Response time, the optimum pH and life time of enzyme immobilized membrane were 2 min, pH 7.3∼7.6 (Dulbecco's buffer solution) and about 25 days respectively. When the enzyme electrode was applied to the $NH_4^+$ determination with amperometric method, other physiological materials had no interference.

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Amperometric Biosensor for Hydrogen Peroxide Determination Based on Black Goat Liver-Tissue and Ferrocene Mediation (흑염소 간-조직과 Ferrocene 매개체를 사용한 과산화수소정량 전류법 바이오센서)

  • Kwon, Hyo-Shik;Park, In-Keun;Kim, Yang-Sug
    • Journal of the Korean Chemical Society
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    • v.48 no.5
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    • pp.491-498
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    • 2004
  • The response characteristics of the bioelectrode developed by the co-immobilization of black goat liver tissue and ferrocene in a carbon paste matrix for the amperometric determination of hydrogen peroxide were evaluated. In the range of electrode potential examined ($-0.3{\sim}+0.0\;V$ vs. Ag/AgCl), the response time was relatively short (t95%=12 s) and it responded in the wide range of pH. The detection limit was 2.25${\times)10^{-6}M$ and a relative standard deviation of the measurements which were repeated 15 times using 1.0${\times}10^{-2 }$M hydrogen peroxide was 1.87%. The bioelectrode sensitivity decreased to 50% of the original value in 19 days of continuous use.

Physiological Response and Hematological Characteristics of Goldfish (Carassius auratus) to Water Temperature Shock (수온충격에 따른 금붕어(Carassius auratus)의 혈액 성상 및 생리적 반응)

  • Hur, Jun Wook;Habibi, Hamid R.
    • Korean Journal of Ichthyology
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    • v.19 no.2
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    • pp.93-100
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    • 2007
  • The effects of sudden changes of water temperature (WT) on the physiological response and hematological characteristics of the goldfish (Carassius auratus) were examined by manipulating WT in a flow through freshwater culture system with tanks. The WT was dropped from $15^{\circ}C$ to $10^{\circ}C$ within 1 hour and then returned to the original water temperature within 12 hours and maintained for 12 hours at the normal WT. The WT stress give continued for 3 days. Plasma levels of cortisol, glucose and lactic acid were higher in stress group than that of non-stressed group until 72 hours. However, The $Na^+,\;Cl^-$, osmolality and aspartate aminotransferase (AST) levels showed no significant differences in two groups. This results in stress group showed that goldfish exhibit "typical" physiological responses (in cortisol, glucose, lactic acid, hematocrit, red blood cell and hemoglobin) to the stress induced by WT changes.

An Effective Defensive Response in Thai Aromatic Rice Varieties(Oryza sativa L. spp. indica) to Salinity

  • Cha-um, Suriyan;Vejchasarn, Phanchita;Kirdmanee, Chalermpol
    • Journal of Crop Science and Biotechnology
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    • v.10 no.4
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    • pp.257-264
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    • 2007
  • Rice is one of the world's staple crops and is a major source of carbohydrate. Rice is exported from several countries, providing a major source of income. There are many documents reporting that rice is a salt-sensitive crop in its developmental stages. The objective of this investigation is to evaluate the effective salt-tolerance defense mechanisms in aromatic rice varieties. Pathumthani 1(PT1), Jasmine(KDML105), and Homjan(HJ) aromatic rice varieties were chosen as plant materials. Rice seedlings photoautotrophically grown in-vitro were treated with 0, 85, 171, 256, 342, and 427 mM NaCl in the media. Data, including sodium ion$(Na^+)$ and potassium ion$(K^+)$ accumulation, osmolarity, chlorophyll pigment concentration, and the fresh and dry weights of seedlings were collected after salt-treatment for 5 days. $Na^+$ in salt-stressed seedlings gradually accumulated, while $K^+$ decreased, especially in the 342-427 mM NaCl salt treatments. The $Na^+$ accumulation in both salt-stressed root and leaf tissues was positively related to osmolarity, leading to chlorophyll degradation. In the case of the different rice varieties, the results showed that the HJ variety was identified as being salt-tolerant, maintaining root and shoot osmolarities as well as pigment stabilization when exposed to salt stress or $Na^+$ enrichment in the cells. On the other hand, PT1 and KDML105 varieties were classified as salt-sensitive, determined by chlorophyll degradation using Hierarchical cluster analysis. In conclusion, the HJ-salt tolerant variety should be further utilized as a parental line or genetic resource in breeding programs because of the osmoregulation defensive response to salt-stress.

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An Experimental Study on the Effect of Sensor Line Number on the Reactivity Characteristic of Corrosion Sensor Reactive with Chloride Ion to Immigrate into Concrete (콘크리트내로 침투하는 염소이온 반응형 부식센서의 응답특성에 미치는 센서 세선 수의 영향에 관한 실험적 연구)

  • Lee, Hyun-Seok;Lee, Han-Seung
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.18 no.3
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    • pp.143-152
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    • 2014
  • In this study, the sensor response and sensitivity is experimented and analyzed quantitatively by the line numbers of chlorine ion reaction type corrosion sensor that is developed. The sensor response of the developed corrosion sensor is verified with properties of chlorine ion. The multilineal sensor is shown a large resistance change more than the single line sensor by damage of the sensor. And, the resistance change of sensor is as large as high concentration of NaCl aqueous solution, the sensitivity of multilineal sensor is higher than single line sensor's, and the depth of sensor's location is as large as the increasing of resistance change time (cycle). These results suggest that, the developed corrosion sensor could sense corrosion reaction, sensor sensitivity and change of resistance for chloride ion. Especially, It was judged that 7 line sensor was the most superior for monitoring chloride ion immigration into concrete.