• Title/Summary/Keyword: CL response

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Effects of Polyethylene Glycol Priming on Seed Germination of the Medicinal Plant, Kenaf (Polyethylene Glycol Priming 처리가 약용작물 케나프 종자 발아에 미치는 영향)

  • Lee, In Sok;Kang, Chan Ho;Lee, Ki Kwon
    • Korean Journal of Medicinal Crop Science
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    • v.26 no.1
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    • pp.55-63
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    • 2018
  • Background: The present study assessed the response of kenaf (Hibiscus cannabinus L., Jangdae) seed to NaCl and the effects of polyethylene glycol (PEG) on kenaf seed germination and vigor. Methods and Results: Seed germination ranged from 11.3% to 58.8% after 24 hours of immersion in NaCl concentrations from 0% to 0.5%. The priming treatments had lower electrical conductivity (EC) values for the seeds than for the control and a deteriorated palisade layer. Priming in 10% PEG for 48 hours increased the germination upto 96.3% in $H_2O$ solution and 98.8% in 0.3% NaCl solution compared to that of the control (78.8%). Germination synchronization, and shoot and root growth of the primed seeds were greater than those of the control. The $T_{50}$ of the control in $H_2O$ and 0.3% NaCl solution was 22 and 28 times, respectively. After priming, nine times was sufficient to reach $T_{50}$ in both solution. The mean number of days to germination (MDG) decreased from 1.43 days for the control to 0.55 days for 0% PEG in $H_2O$ solution and from 1.57 days for the control to 0.56 days for 0% PEG in 0.3% NaCl solution. The dry weight after the 10% PEG treatment was higher than that of the control. Conclusions: Taken together, 10% PEG treatment for 24 hours is recommended for kenaf seed invigoration before planting.

Comparative study on the properties of polypeptides induced by NaCl, drought and temperature treatments in rice seedlings (NaCl, 한발 및 온도 처리에 따른 유묘기 수도의 폴리펩티드 속성의 비교분석)

  • Lim, Gum-Chun;Jung, Yeoung-Sang;Shin, Jeong-Sheop
    • Applied Biological Chemistry
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    • v.35 no.6
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    • pp.485-489
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    • 1992
  • Plants are altered not only in the outward appearance but also in their physiological and biochemical properties with reaction to the environmental stresses; particularly, the biosynthetic system of protein in situ rapidly responds to this. In order to investigate the change of properties of polypeptides in rice plants induced by several stresses, the seedlings were subjected to exposure to NaCl, drought, and low and high temperatures, respectively, and then some aspects of polypeptide variations were compared. Without exception, the rice plant, which is somewhat tolerant to environmental change, shows the alteration in several polypeptides. Moreover, newly synthesized polypeptides were observed in response to stresses. The existing proteins for the primary metabolic pathways were markedly decreased as each treatment progressed.

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A Prospective Study on Ginseng Intake and Cancer for Population -Preliminary Report- (인삼복용과 암에 관한 전향성 역학적 연구 - 1차 보고 -)

  • Yun, Taik-Koo;Choi, Soo-Yong;Lee, Yun-Sil
    • Journal of Ginseng Research
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    • v.19 no.2
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    • pp.87-92
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    • 1995
  • We conducted a prospective cohort study to evaluate the preventive effect of ginseng on the development of cancer in the population over 40 years old residing in Kangwha-eup from August 1987 to December 1992. Alnong 4, 634 persons (2, 362 men, 2, 272 women), 335 (7.6%) persons had died. Cancer accounted for 79 (22.8%) of the total death. Of 4, 634 persons eligible for analysis, 70.475 (3, 263) were ginseng intakes. Ginseng intakes had a decreased risk (RR=0.48, 95% Cl : 0.34~0.67) compared with non-intakes. The relative risk of cancer according to the kind of ginseng was 0.23 (95% Cl : 0.08~ 0.63) for fresh ginseng extract intakes. There was a decrease in risk with rising the frequency of ginseng intake, showing statistically significant dose-response relationship. The longer the duration of ginseng intake or the greater the total number of ginseng consumed, the lower the risk. Newly diagnosed cancer cases have been identified: 42 stomach, 24 lung, 14 liver and 57 at other sites. The relative risks of ginseng intakes were 0.34 (95% Cl 0.19~0.60) in gastric cancer and 0.27 (95% Cl : 0.12-0.60) in lung cancer. Among ginseng preparations, fresh ginseng intakes were significantly associated with the decreased risk of gastric cancer (RR: 0.19, 95% Cl : 0.04~0.98). These results strongly revealed that Panax Ginseng C.A. Meyer (Korean ginseng) has preventive effect against cancer.

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Development of Multi-rod Type Ag-AgCl Electrodes for an Underwater Electric Field Sensor (수중 전기장 센서용 다중 막대형 은-염화은 전극 개발)

  • Lee, Sangkyu;Yang, Chang-Seob;Chung, Hyun-Ju
    • Journal of Sensor Science and Technology
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    • v.31 no.1
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    • pp.45-50
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    • 2022
  • Multi-rod type Ag-AgCl electrodes have been developed for use in underwater electric field sensors. The developed cylindrical electrode had a diameter of 50 mm and a height of 130 mm. The electrode had five Ag-AgCl rods with a diameter of 2 mm and a height of 80 mm to enlarge the reaction surface area. Each Ag-AgCl rod was fabricated under the same conditions as the usual anodizing method in an electrolyte. The two developed electrodes were placed in the center of a 500-mm long, 400-mm wide, and 300-mm high acrylic tank filled with artificial seawater, at an interval of 100 mm, to evaluate their characteristics as uniaxial underwater electric field sensors. The underwater external electric field was generated using titanium plate electrodes installed at both ends of the tank. The noise level at 1 Hz of the developed electrode was approximately 3.7 nV/√Hz. The reception of the underwater electric field signal using the developed electrode was linear, within an error of approximately 0.6 %, in the range of 1-10000 ㎶/m at 1 Hz. In addition, its frequency response was flat within an error of 1.1 % in the range of 1-1000 Hz at 10000 ㎶/m.

Polypyrrole Modified Electrode as a Nitrate Sensor

  • Sung Chul Kang;Keun-Sun Lee;Jin-Doo Kim;Kang-Jin Kim
    • Bulletin of the Korean Chemical Society
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    • v.11 no.2
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    • pp.124-126
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    • 1990
  • The potentiometric response behavior of a polypyrrole(PPy) coated Pt electrode to nitrate ion has been studied. The electrode shows a nernstian behavior with a slope of 59 mV over 0.50 M to $1.0{\times}10^{-3}M\;NO_3\;^-$ and a detection limit of $1.0{\times}10^{-4}M\;NO_3\;^-$. The response of the electrode is fast and the selectivities for $I^-,\;ClO_4\;^-,\;and\;IO_4\;^-$ are found to be improved. The effect of pH on the potential response to $NO_3\;^-$ is compared with the existing nitrate ion selective electrodes.

Effects of NaCl Concentration on the Growth of Native Willow Species Collected in a Coastal Reclaimed Land (간척지 자생 버드나무의 NaCl 농도별 생육반응)

  • Yeo, Jin-Kie;Park, Jung-Hyun;Koo, Yeong-Bon;Kim, Hyun-Chul;Shin, Han-Na
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.2
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    • pp.124-131
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    • 2010
  • This study was conducted to investigate the potentials for the forest restoration on reclaimed land by using willow trees (Salix koreensis Anderson) selected from a coastal reclaimed land made in inside of the Sihwa tide embankment. We first collected six individual willow trees that were the only tree species grown in the reclaimed land. Total 7 clones from cuttings of the collected trees and the control were grown in a greenhouse for two months prior to applying the different concentrations of NaCl solutions (0.0%, 0.1%, 0.5%, and 1.0%). One month after the NaCl application, the survival rates of clones from both the collected trees, and the control were significantly decreased in a NaCl dose-dependent manner. However, there was no significant difference between the collected trees and the control in terms of survival rate, hight and diameter of cuttings, and the numbers of leaves in greenhouse condition. In conclusion, the willow trees collected from the coastal reclaimed land showed no tolerance against NaCl compared to the control grown in ordinary soil, suggesting that further study is required to determine what the most important factor is to select salt tolerant tree species.

Effects of Serum on Nitric Oxide Production in Embryonic Mouse Liver Cell Line BNL CL.2 (혈청이 마우스 간 세포주 BNL CL.2의 Nitric Oxide 생성에 미치는 영향)

  • 김유현;김신무;배현옥;유지창;정헌택;진효상
    • Biomedical Science Letters
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    • v.5 no.1
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    • pp.85-93
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    • 1999
  • Nitric oxide (NO) plays an important role in immunologic defense, and influences upon the functioning of secretory tissues and cells. It also exhibits cytotoxic/cytostatic activity as one of major operating effectors of the cellular immunity system. We investigated the effects of serum on the cell damages and NO production in the mouse liver cell line BNL CL.2 to establish the role of NO. We observed that, when BNL CL.2 cells were cultured in serum-free medium, they were induced to cell damage by the stimulation of IFN-$\gamma$ alone or IFN-$\gamma$ plus LPS. Serum-starved cells showed large amount of nitrite accumulation and NO synthase (NOS) expression in response to IFN-$\gamma$ alone in dose- and time- dependent manners, but serum-supplied cells did not The production of NO was blocked by protein tyrosine kinase (PTK) inhibitors, genistein and herbimycin. These results suggest that the deprivation of serum in the BNL CL.2 cell culture medium might primed with the cells to produce NO when the cells are triggered by IFN-$\gamma$ and the involvement of PTK signal transduction pathway in the expression of NOS gene in murine hepatocytes.

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Evaluation of Salt Tolerance of Liriope platyphylla and Pachysandra terminalis to Deicing Salt (CaCl2) Concentration in Winter (겨울철 제설제(CaCl2)농도처리에 따른 맥문동과 수호초의 내염성 평가)

  • Ju, Jin-Hee;Hui, Xu;Park, Ji-Yeon;Choi, Eun-Young;Yoon, Yong-Han
    • Korean Journal of Environment and Ecology
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    • v.30 no.4
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    • pp.651-657
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    • 2016
  • It is important to know know deleterious impact of deicing salt on plants for guidelines of planting along roads. The aim of this study was to determine the effect of calcium chloride ($CaCl_2$) on the growth and physiological characteristics of Liriope platyphylla and Pachysandra terminalis. The plants were grown from November of 2015 to March of 2016 in pots containing growing media with $CaCl_2$ at 0% (Control), 0.5%, 1.0%, 3.0%, and 5.0% (based on the weight). While plant growth and photosynthetic activity were significantly decreased in both plant species grown on the media with $CaCl_2$, the degree of sensitivity to $CaCl_2$ differed. The plant growth of Liriope platyphylla was considerably injured under higher than 3.0% of $CaCl_2$, whereas Pachysandra terminalis was all dead under higher than 1.0% of $CaCl_2$. This results indicate that Liriope platyphylla has higher degree of tolerance to the deicing salt than Pachysandra terminalis.

Growth and Physiological Characteristics in a Halophyte Suaeda glauca under Different NaCl Concentrations (염생식물 나문재의 염농도에 따른 생장 및 생리적 특성)

  • Kim, Ji-Young;Seong, Phil-Mo;Lee, Deog-Bae;Chung, Nam-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.64 no.1
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    • pp.48-54
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    • 2019
  • This research was carried out to investigate the plant growth, inorganic ion and amino acid content characteristics in a halophyte, Suaeda glauca, under different NaCl concentrations for cultivating in the reclaimed land. S. glauca was hydroponically cultivated under 0, 50, 100, 200, 300 and 400 mM NaCl concentrations with Hogland's nutrient solution. To evaluate growth response under different NaCl concentrations, plant height and number of branches, dry weight, Fv/Fm value, and photosynthetic efficiency were investigated. To find out physiological characteristic, inorganic ion contents and amino acids in the plant were evaluated. The optimum concentration of NaCl for plant growth were 50 mM. The plant growth were gradually decreased in the concentration ranged from 100 to 400 mM. As increasing of NaCl concentration, Na ion was increased, but K, Ca, Mg ions were decreased in the plant. The amino acid contents were varied due to NaCl concentrations, but most of amino acids content in total plant was the lowest at 50 mM. Conversely, proline was exceptionally high at 50 mM of NaCl concentration. The Fv/Fm value was the highest at 50 mM of NaCl concentration. From these results, the optimum salt concentration for the growth of S. glauca was 50 mM, but the plant seems to adapt in a variety of salt environments in view of the change of ions and amino acids depending on salt concentration and the maintenance of photosynthetic efficiency even under high salt condition.

The effects of Bo-joong-ik-gi-tang administration on Metabolic Responses ; changes in the energy and electrolyte metabolism among long distance runners (보중익기탕(補中益氣湯) 투여가 장거리 달리기 선수의 에너지 및 전해질 대사에 미치는 영향)

  • Song, Sun-Gi;Keum, Dong-Ho;Oh, Jae-Geun;Lee, Myeong-Jong
    • The Journal of Dong Guk Oriental Medicine
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    • v.7 no.1
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    • pp.99-117
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    • 1998
  • Objective This experimental study was designed to investigate effects of Bo-joong-ik-gi-tang administration among long distance runners on changes of the energy and electrolyte metabolism. Materials and Methods All subjects were divided randomly with two groups, Bo-joong-ik-gi-tang Group (N=4) and control group (N=4) and performed to run the 400m track with 70% of HR max about 1 hour. The blood samples were collected from antecubital vein by 5ml syringes at before exercise, immediately after exercise, recovering-10 min. recovering-30 min, recovering-1 hour. These samples were used to analyze for the factors of the change on metabolic responses. First, the primary factors on the changes of the energy metabolism were checked ; Glucose, Free fatty acid, Lactate, LDH. Second, the primary factors on the changes of the electrolyte metabolism were checked ; Na+, CI-. K+. Results 1. The change of the energy metabolism 1) Glucose response was not shown significant difference between two groups. 2) Free fatty acid response in Bo-joong-ik-gi -tang group was significantly increased at recover-10 min. 3) Lactate response in Bo-joong-ik-gi-tang group was significantly decreased at immediately after exercise, recover-10 min, recover-30 min. 4) LDH response was not shown significant difference between two groups. 2. The change of the electrolyte metabolism 1) Na+ response in Bo-joong-ik-gi-tang group was shown significant difference between two groups at before exercise. 2) Cl- response in Bo-joong-ik-gi-tang group was significantly increased at before exercise, immediately after exercise. 3) K+ response in Bo-joong-ik-gi-tang group was significantly increased at recover-10 min. Conclusion According to the above results, it was shown that Bo-joong-ik-gi-tang had the positive effects on changes of the energy and electrolyte metabolism for the long distance runners.

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