• 산경연구논집
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• 제10권2호
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• pp.25-37
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• 2019

Purpose - The goal of this study is to analyze the differences in education performances between students of the government's financial support program and those who do not receive support at a local university in Korea. Research design, data, and methodology - The questionnaire used was NASEL. NASEL is considered a highly suitable survey tool for professors, courses, and performances in Korean universities. The 290 students who participated and 44 students do not participate in the financial support program were surveyed for 10 days. The characteristics of students were investigated by frequency analysis and technical statistics. The analysis of student collective characteristics used independent t and f-tests,and one-way ANOVA with IBM SPSS Statistics 22.0 for statistical purposes. Results - The p-value of the group receiving financial support and the group without financial support in active-collaborative learning is 0.167. The p-value of the economically supported group and the non-supported group of the faculty-student interaction is 0.281. The confidence coefficient of the active-collaborative learning questionnaire is 0.861. The reliability coefficient of the questionnaire for the faculty-student interaction questionnaire is 0.871. Conclusions - There are no clear differences in active-collaborative learning and faculty-student interaction between participating and non-participating students in the economic program.

• Steel and Composite Structures
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• 제14권2호
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• pp.133-153
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• 2013

The concrete-filled steel tube (CFT) columns have several benefits of high load-bearing capacity, inherent ductility and toughness because of the confinement effect of the steel tube on concrete and the restraining effect of the concrete on local buckling of steel tube. However, the experimental research into the behavior of square CFT columns consisting of high-strength steel and high-strength concrete is limited. Six full scale CFT specimens were tested under flexural moment. The CFT columns consisted of high-strength steel tubes ($f_y$ = 325 MPa, 555 MPa, 900 MPa) and high-strength concrete ($f_{ck}$ = 80 MPa and 120 MPa). The ultimate capacity of high strength square CFT columns was compared with AISC-LRFD design code. Also, this study was focused on investigating the effect of high-strength materials on the structural behavior and the mathematical models of the steel tube and concrete. Nonlinear fiber element analyses were conducted based on the material model considering the cyclic bending behavior of high-strength CFT members. The results obtained from the numerical analyses were compared with the experimental results. It was found that the numerical analysis results agree well with the experimental results.

• 한국항해항만학회지
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• 제39권2호
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• pp.99-105
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• 2015

Until recently, thermal power plants have used high-rank coals to generate electricity. The switched to low-rank coals, primarily because of the rising coal price and the advancement of combustion technology. Therefore the thermal power plants need more fuels aspect of quantity and they are going to build extra infrastructure to deal with the increased fuel demand in their specialized ports. This paper introduces the process of the economic analysis as a case study for Hadong T/P(Thermal Power) Port. This study also evaluates investment for mew projects in ports. We analyze the costs and benefits of the port investment project using various information. And then we conduct the economic analysis using NPV(net present value), B/C ratio and IRR grounded in a financial theory. Out result of the economic feasibility shows that the new project of constructing a third berth in Hadong T/P Port has positive economic value. Additionally, this study conducts the sensitivity analysis of the major variables like cost, benefit and discount rate.

• Preventive Nutrition and Food Science
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• 제11권2호
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• pp.127-132
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• 2006

Bacillus sp. strain K-l, which produces a strong fibrinolytic enzyme, was isolated from chongkukjang, a traditional Korean fermented soybean paste. The fibrinolytic enzyme was purified from chongkukjang base by using ammonium sulfate fractionation and chromatographic techniques. Purified enzyme, CK K-1 was demonstrated to be homogeneous by SDS-PAGE and isoelectric focusing electrophoresis, and has molecular mass of a 12.4 kDa and a pI of 8.0. The optimal reaction pH value and temperature were 8.0 and $40^{\circ}C$, respectively. Phenyl-methyl-sulfonyl-fluoride (PMSF; serine protease inhibitor), ethylene-diamine-tetra-acetic acid (EDTA; metallo protease inhibitor), copper ion, ferric ion and lead ion inhibited the enzyme activity. These results indicated that the fibrinolytic enzyme is a metallo-serine protease and different from nattokinase and chongkukjangkinase.

• Bioinformatics and Biosystems
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• 제2권1호
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• pp.28-32
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• 2007

The protein phosphorylation is one of the important processes in the cell signaling pathway. A variety of protein kinase families are involved in this process, and each kinase family phosphorylates different kinds of substrate proteins. Many methods to predict the kinase-specific phosphoryrated sites or different types of phosphorylated residues (Serine/Threonine or Tyrosin) have been developed. We employed Supprot Vector Machine (SVM) to attempt the prediction of protein kinase specific phosphorylation sites. 10 different kinds of protein kinase families (PKA, PKC, CK2, CDK, CaM-KII, PKB, MAPK, EGFR) were considered in this study. We defined 9 residues around a phosphorylated residue as a deterministic instance from which protein kinases determine whether they act on. The subsets of PSI-BALST profile was converted to the numerical vectors to represent positive or negative instances. When SVM training, We took advantage of multiple SVMs because of the unbalanced training sets. Representative negative instances were drawn multiple times, and generated new traing sets with the same positive instances in the original traing set. When testing, the final decisions were made by the votes of those multiple SVMs. Generally, RBF kernel was used for the SVMs, and several parameters such as gamma and cost factor were tested. Our approach achieved more than 90% specificity throughout the protein kinase families, while the sensitivities recorded 60% on average.

• 대한수의학회지
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• 제60권3호
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• pp.109-116
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• 2020

This study aimed to develop a semi-nested polymerase chain reaction assay for the direct detection of Mycoplasma synoviae (M. synoviae) from clinical samples using three newly designed oligonucleotide primers specific to the variable lipoprotein haemagglutinin (vlhA) gene and differentiate M. synoviae field strains based on a nucleotide deletion or the insertion of the proline-rich repeat (PRR) region of the vlhA gene. The developed semi-nested polymerase chain reaction (PCR) assay revealed positive results in 12 out of 100 clinical samples collected from chickens showing lameness and joint swelling. Six positive samples were selected randomly for sequencing, and sequence analysis revealed 96.3-100% nucleotide identities compared to the reference sequences. Phylogenetic analysis showed that sequences of the strains in this study were closely related to WVU1853 (Spain), CK.MS.UDL.PK.2014.2 (Pakistan), and F10-2AS (USA) strains, but they were distinct from the M. synoviae-H vaccine strain sequence. M. synoviae obtained from these samples were identified as types A and C with a length of 38 and 32 amino acids, respectively. These results indicated that the specific and sensitive semi-nested PCR could be a useful diagnostic tool for the direct identification of clinical samples, and the sequence analysis of the partial vlhA gene can be useful for typing M. Synoviae.

• 한국어병학회지
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• 제17권2호
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• pp.113-121
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• 2004

This study was performed to investigate the pathphysiological side effects of chloramphenicol (CAP) on olive flounder, Paralichthys olivaceus. 150 mg/kg/bw/day of CAP was orally administered forcedly to flounder for 4 days and then the flounder was monitored for 11 days. The level of hematocrit was significantly decreased from 2 day to 3 day of CAP treatment in CAP-treated group. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and gamma glutamyl transferase (GGT) were slightly increased during the treatment of CAP and gradually decreased after the treatment of CAP. The levels of blood urea nitrogen (BUN) and creatinine kinase (CK), although not significant, were slightly increased during the treatment of CAP. The level of lactate dehydrogenase (LDH) was not changed during the treatment of CAP but slightly increased after the treatment of CAP. However, no histopathological changes were observed in various organ after the treatment. From these results, it is suggest that CAP can evoke several side effects on haematopoietic organs in olive flounder.

• 대한안전경영과학회지
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• 제13권2호
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• pp.203-210
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• 2011

Incheon Port has been served as not only the center of the Yellow Sea and port for trade with China but also domestic gateway port for the metropolitan area, playing a professional role as the greatest domestic Port which processes a variety of items such as imported raw materials, bulk cargo, oil and gas and so on. This study was conducted based on step by step business process between port entry and departure of ship, and incurred cost according to port industry classification and performance on the port facility. Bulk cargo and general cargo such as grain, food, steel, scrap metal, wood, coal, LNG, LPG and oil etc., which have a vast majority of import goods, have been treated by 57,062 thousand tons and 7,409 thousand tons of container cargo were processed at Incheon Port in 2009. Bulk vessels and carriers moving items such as LPG, LNG and oil recorded 38,836 thousand won and full-container vessels recorded 11,001 thousand won as the amount of notified port facility usage fees in 2008.

• Journal of Forest and Environmental Science
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• 제39권2호
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• pp.96-104
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• 2023

The successful restoration program requires a comprehensive understanding of variables influencing seedling efficiency. Below-ground is hypothesized to have a major impact on seedling performance of species when planted in agriculture, and degraded areas with different types of mulching. This study investigated on Sg. Terla Forest Reserve in Cameron Highlands Pahang, Malaysia. In this study randomized complete block design (RCBD) was used. The excavation method was applied to study the root system development, above, and below ground biomass distributions under different types of mulching: coconut mulching (CM), oil palm mulching (OM), plastic mulching (PM) and control (CK). The root diameter, main root length, lateral root length, root coiling, and root direction toward to sun were recorded. The results in this study indicate that mulching had significant effect on root diameter, main root length, and root distributions among treatments while for lateral root length, root: shoot ratio, dry biomass distributions, and above and below ground biomass did not showed significant effect among treatments. The highest values for root diameter, lateral root length, main root length, root distributions, dry biomass distributions and above and below ground biomass were showed in CM treatments. However 75% of root coiling was observed in seedlings between treatments.

• 한국발생생물학회지:발생과생식
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• 제11권2호
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• pp.85-96
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• 2007

중간엽 줄기세포(MSC)를 체외배양할 때 사용하는 소태아혈청 (FBS)의 생물학적 불안전성은 이를 임상적으로 사용하는데 있어 제한점으로 작용한다. 본 연구에서는 소태아혈청 및 인간의 제대혈청을 사용하여 인간의 양막유래 중간엽 줄기세포 (HAM)를 각각 배양한 후, 세포의 성장속도와 유전자 및 단백질의 발현 양상을 비교하였다. 제왕절개 후 얻은 양막으로부터 HAM을 분리하여 10% FBS, 5% HCS 혹은 10% HCS가 각각 첨가된 DMEM 배양액에서 배양하였으며, 초기와 후기 계대의 세포를 얻어 이들의 생물학적 특성을 비교 분석하였다. 역전자 중합효소반응 결과, 혈청의 종류에 상관없이 배양된 세포들은 모두 OCT-4, Rex-1, SCF, FGF-5, BMP-4, nestin, NCAM, GATA-4, HLA-ABC 유전자를 발현하였으며, 이러한 발현은 초기 및 후기 계대의 세포에서도 마찬가지로 나타났다. 세포면역화학 반응 결과, FBS 혹은 HCS를 첨가한 배양액에서 배양된 HAM은 4번째 계대에서 collagen I, II, III, XII, fibronectin, $\alpha$-smooth muscle actin, vimentin, CK18, CD54, FSP, TRA-1-60, SSEA-3, -4, HLA ABC 단백질을 뚜렷하게 발현하였다. 그러나 desmin 단백질은 FBS가 첨가된 배양액에서 배양된 HAM에서만 발현되었고 vWF 단백질은 HCS가 첨가된 배양액에서 배양된 HAM에서만 발현되었다. 결론적으로 유전자와 단백질의 발현양상을 살펴본 결과, HCS가 첨가된 배양액에서 배양된 HAM은 전형적인 인간성체줄기세포의 특징을 나타내고 있으며, FBS가 첨가된 배양액과 비교하여 동등한 성장 촉진 효과를 가지는 것으로 보인다.