• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.4
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• pp.701-706
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• 2002

CheonghunHwadam-tang(CHT) have been used in oriental medicine for many centuries as a therapeutic agent of vertigo by wind, fire and phlegm. CHTS was CHT adding Schizonepetae Herba. The effects of CHTS on the cerebral blood flow and blood pressure is not known. The purpose of this Study was to investigate effects of CHTS on the regional cerebral blood flow(rCBF) and mean arterial blood pressure(BP), action-mechanism of CHTS-induced changed-rCBF and BP. The changes of rCBF and BP was determinated by Laser-Doppler Flowmetry(LDF). The results were as follows ; CHTS extract was increased significantly rCBF in a dose-dependent, but was not changed BP compared with CHTS non-treated group. Pretreatment with propranolol, indomethacin and methylene blue were inhibited CHTS induced increase of rCBF, propranolol(all CHTS-treated group) and indomethacin(CHTS 0.01 mg/kg) of them were significantly decreased. Pretreatment with propranonol and indomethacin were inhibited CHTS induced increase of BP, but pretreated with methylene blue was significantly accelerated BP in high dosage. This results suggest that CHTS increased rCBF by dilating pial arterial diameter and the action of CHTS is also mediated by adrenergic β -receptor and cyclooxygenase.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.3
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• pp.746-750
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• 2003

Cheonghunhwadam-tang have been used in oriental medicine for many centuries as a therapeutic agent of vertigo by wind, fire and phlegm. CHT Gamypang(CTG) was CHT adding Aurantii Fructus(AF). The effects of CTG on the regional cerebral blood f1ow(rCBF) and mean arterial blood pressure(MABP) is not known. The mechanical Study of CTG on the cerebral hemodynamics is not known too. Therefore, purpose of this Study was to investigate effects of CTG on the rCBF and MABP, mechanism of CTG on the cerebral hemodynamics in rats. The changes of rCBF and BP was determinated by Laser-Doppler Flowmetry(LDF). The results were as follows ; CTG extract was significantly decreased rCBF in a dose-dependent, but the change of MABP was not shown. Pretreatment with propranolol(3mg/kg, i.v.) was significantly increased CTG(10 mg/kg, i.v.) induced decrease of rCBF. Pretreatment with indomethacin(3mg/kg, i.v.) and methylene blue(10㎍/kg, i.v.) were increased CTG induced decrease of rCBF too. This results suggest that the mechanism of CTG is mediated by adrenergic β - receptor, guanylate cyclase and cyclooxygenase.

• Korean Journal of Food Science and Technology
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• v.25 no.5
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• pp.571-575
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• 1993

Effect of surface hydrophobicity of soybean peptides on serum cholesterol in rats was investigated. Soybean protein(ISP), casein(CNP), and their peptic hydrolyzates fractionated by acid precipitations (SHT, SH8, SH6, SH4, CHT, CH6, CH5, CH4) were fed to rats and the concentration of serum cholesterol and the fecal steroid excretion were measured. And surface hydrophobicities of the peptide fractions were measured by determining by the ANS flourescence intensity and SDS binding capacity. It was found that the higher the surface hydrophobicity of peptides was, the more the fecal steroids excreted(r=0.801) and the lower the concentration of serum cholesterol became(r=-0.868). However, there was no relationship between SDS surface hydrophobicity and fecal steroids or serum cholesterol. ANS surface hydrophobicity of soybean protein was increased by enzymatic hydrolysis. These results suggest that high surface hydrophobicity of peptides formed during digestion is responsible for the hypocholestrolemic effect of soybean protein through the hydrophobic interaction between the peptides and bile salts in rats.

• Korean Journal of Food Science and Technology
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• v.25 no.5
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• pp.552-557
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• 1993

Effects of amino acid composition and average hydrophobicity of soybean peptides on serum cholesterol in rats were investigated. Soybean protein(ISP), casein(SCN), their peptic hydrolyzates fractionated by acid precipitations(SHT, SH8, SH6, SH4, CHT), and amino acid mixtures of the same composition as the proteins(SAA, CAA) were prepared to feed to rats. The amino acid composition of the peptides was analyzed by HPLC and the concentration of serum cholesterol in the rats was measured. By data analysis, it was found that there was no relationship between ratio of Lys/Arg or molar ratio of hydrophobic amino acids and serum cholesterol level. And also there was no relationship between the concentration and average hydrophobicity calculated by the method of Tanford, Manavalan, for Meirovitch, only except by the method of Krigbaum(r=-0.736); the higher the average hydrophobicity of Krigbaum was, the lower the concentration of serum cholestrol became.

• The Korea Journal of Herbology
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• v.34 no.4
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• pp.9-18
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• 2019

Objectives : It has been known to be correlated between phlegm and dementia from the perspective of oriental medicine, but it is unexplored whether herbal medicines to treat phlegm have pharmacological actions on Alzheimer's disease (AD). The aim of this study was to evaluate and to compare effects of herbal medicines to treat phlegm against AD in vitro. Methods : We selected 11 herbal medicines which treat phlegm and obtained each extract by boiling in 10-fold distilled water for 2 h. And we performed the assay of acetylcholinesterase (AChE) inhibitory effects of 11 herbal extracts. Next, we evaluated neuroprotective effects of them against amyloid $beta_{25-35}$ ($A{\beta}_{25-35}$) plaque-induced toxicity in HT22 mouse hippocampal neuronal cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. To investigate whether they show the anti-inflammatory effects against lipopolysaccharide (LPS), we also measured the levels of nitric oxide (NO) in BV2 microglia cells using griess reagent assay. Results : We found that Gamiyeongsin-hwan (GYH) and Cheonghunhwadam-tang (CHT) exhibited remarkable AChE inhibitory effects. In HT22 cells, Arisaematis Rhizoma, Trichosanthis Semen and Fritillariae Thunbergii Bulbus suppressed $A{\beta}_{25-35}$ plaque-induced neuronal cell death. In BV2 cells, Cheongung-hwan significantly inhibited the increase of NO contents induced by LPS and GYH and CHT showed a tendency to inhibit LPS-induced NO generation. Conclusions : These results suggest that several herbal medicines to treat phlegm showed the significant effects on AChE inhibition, neuroprotection against $A{\beta}_{25-35}$ plaque-induced toxicity, and inhibition of NO generation. Therefore, we demonstrate the possibility that herbal medicines with treating phlegm has effects against AD.

• Journal of Software Assessment and Valuation
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• v.16 no.2
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• pp.135-142
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• 2020

In this paper, we describe the iris localization method in RGB images. Most of the iris localization methods are developed for infrared images, thus an iris localization method in RGB images is required for various applications. The proposed method consists of four stages: i) detection of the candidate irises using circular Hough transform (CHT) from an input image, ii) detection of a pupil center based on deep learning, iii) determine the iris using the pupil center, and iv) correction of the iris region. The candidate irises are detected in the order of the number of intersections of the center point candidates after generating the Hough space, and the iris in the candidates is determined based on the detected pupil center. Also, the error due to distortion of the iris shape is corrected by finding a new boundary point based on the detected iris center. In experiments, the proposed method has an improved accuracy about 27.4% compared to the CHT method.

• Research in Plant Disease
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• v.28 no.2
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• pp.82-91
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• 2022

Recently, several in vitro studies have reported antiviral activity of agents of systemic acquired resistance against plant virus infection, but the approach has not been applied in a wide range of agricultural fields. The objective of this study was to evaluate the inhibitory effect of the exogenous application of salicylic acid (SA), chitosan (CH), or eugenol (EG) in tomato yellow leaf curl virus (TYLCV) infection of greenhouse-grown tomato plants. In vitro, the initial time of symptom was observed in TYLCV-infected plants (VP) of the resistant cultivar 'Superdotaerang' at 12 days post inoculation (dpi) after application of antiviral agents. At 32 dpi, the disease rate of TYLCV in the CHT+VP (0.1% chitosan and virus infected control) treated plants was 87.5%, lower than that of the other treatment. However, the virus content in the CHT+VP treated plants was higher than those of the other treatments, and SA, EG, and CH did not show significant effect on plant height or shoot and root fresh weight. Our results from summer-cultivated greenhouse-grown tomatoes show that none of the tested agents had an inhibitory activity on viral infection or yield of tomato 'Dotaerangsola'cultivar. In contrast, all treated 'TY Giants' cultivars that possessed TYLCV resistance genes Ty-1 and Ty-3a did not show typical symptoms and the virus content was remarkably lower than those in the TYLCV treated plants in 'Superdotaerang'. The results of this research indicated that the planting of resistant tomato cultivars was effective method instead of using SA, EG, and CH (known as resistance-inducing factors for control) of TYLCV in the field.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.3 no.1
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• pp.37-47
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• 1995

It is very important to determine the performance and operating envelope of engine for aircraft's flight. The basic performance is provided by manufacturer, but installed engine's operating envelop is only determined by flight test. First, this study was measured cylinder head temperature(CHT), exhaust gas temperature(EGT) and oil temperature. At pre-determined altitude and power rate, these temperatures were measured by change of mixture ratio and mixture ratio condition for economic operation and max. power were found. And secondly, with the measured temperatures, possible positions of mixture lever were determined by flight test for stable flight Chang-91 and Lycoming IO-360-A series four cylinders engine with 200 hp was used for this study.

• 한국전산유체공학회:학술대회논문집
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• 2010.05a
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• pp.468-472
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• 2010

To develop the Exhaust Maniverter(Manifold and converter) it needs to consider flow characteristics related to durability and performance of the catalyst, manufacturability, etc. This paper presents the analysis results regarding to flow characteristics such as flow uniformity, tangential speed, O2 sensor sensitivity and CHT (conjugate heat transfer) for the LCF(Low Cycle Fatigue) for gasoline 2.0 liter engine. The results are satisfactorily corresponded to the general criteria. Also skin temperature and pressure drop wire measured at the Engine Bench. These results can be useful for the design guide for Exhaust Maniverter.

• Proceedings of the Microbiological Society of Korea Conference
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• 2006.05a
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• pp.97-99
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• 2006

A chitinase-producing bacterium strain KCTC10714 was isolated from sea sand around the King Sejong Station, King George Island in Antarctica. It was identified as Sanguibacter sp., based on the biochemical properties and 16S rRNA gene sequence. KCTC10714 chitinase showed enzyme activity in broad range of temperature from 0 to $70^{\circ}C$. At $0^{\circ}C$, it showed 70.9% of relative activity in comparison with 100%. The chitinase gene of KCTC10714 was cloned using inverse PCR cloning method. KCTC10714 chitinase gene was designated as chi21702. The ORF of chi21702 consisted of 1,449 bp (482 amino acid), and contained ChtBD3 (a chitin/cellulose binding domain) and an active site for chitinase family 18.