• Journal of the Korean Society for Marine Environment & Energy
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• v.10 no.3
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• pp.155-166
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• 2007

We carried out a study on the marine environment, such as water temperature, salinity, density and chlorophyll ${\alpha}$, and the distribution of phytoplankton community, such as species composition, dominant species and standing crops in the Southwestern Sea of Korea during early summer 2005. According to the analysis of a T-S diagram, three characteristics of water masses were identified. We classified them into Korean and Chinese coastal water, the cold water and the oceanic water. The first was characterized by high temperature and low salinity in the surface layer influenced by river run offs from China and Korea, the second by low temperature and salinity in bottom layer originated from the bottom cold water of the Yellow Sea, and the third by high temperature and high salinity influenced by Tsushima warm currents. The internal discontinuous layer among them was formed at the intermediate depth (about $10{\sim}20\;m$ layer). And the thermal front appeared in the central parts between Tsushima warm currents and Korean and Chinese coastal waters in the Southwestern Sea of Korea. Chlorophyll ${\alpha}$ concentration was high values in the Korean coastal waters and sub-surface layers. But It was low concentration in the Tsushima warm currents regions. The $Chl-{\alpha}$ maximum layers appeared in the sub-surface layer below thermocline. The phytoplankton community in the surface and stratified layers was composed of a total of 40 species belonging to 26 genera. Dominant species were 2 diatoms, Paralia sulcata, Skeletonema costatum and a dinoflagellate, Scripsiella trochoidea. Standing crops of phytoplankton in the surface layer were very low with cell density ranging from 5 to $3.8\;{\times}\;10^3\;cells/L$. Diatoms were controlled by the expanded low salinity coastal waters of the low salinity with high concentrations of nutrients. Otherwise phytoflagellates were dominant in the high temperature regions where the Tsushima warm currents approches the Southwestern Sea of Korea in early summer.

• Korean Journal of Environmental Agriculture
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• v.37 no.3
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• pp.229-234
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• 2018

BACKGROUND: in vitro micronucleus test (vitMNT) is one of the promising alternative testing methods in genotoxicity test and was adopted as OECD test guideline for chemical registration. This study was conducted to optimize the cytoplasm conditions in vitMNT using Chinese hamster lung (CHL) cell. METHODS AND RESULTS: In this study cytokinesis-block micronucleus test was conducted. Mitomycin C and colchicine were used as positive control chemicals and were treated for three hours (short time) or twenty-four hours (long time). Giemsa solution was used for cell staining. For optimization of vitMNT, the final fixative was prepared as five concentrations (0%, 1%, 3%, 5%, and 25%) of acetic acid in methanol, and treatment times of the final fixative were varied under four conditions (immediately, one hour, four hours, and one day). CONCLUSION: Acetic acid at 1% in methanol as the final fixative was most adequate to preserve the cytoplasm around the nucleus in the interphase cells. Also, fixative treatment time of cell suspension for one to four hours may minimize the cell rupture. These results can be helpful for getting an accurate result promptly due to clear visual distinction to score micronucleus in vitMNT using giemsa solution.

• Biomolecules & Therapeutics
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• v.2 no.3
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• pp.286-291
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• 1994

The mutagenicity of DA-3030(rhG-CSF)was studied by reverse mutation test, chromosome aberration test and micronucleus test. The reverse mutatuon test in bacteria was performed using salmonella typhimurium strain TA100, TA98, TA1535 and TA1537 with rhG-CSF in any of the concentrations(150, 75, 37.5, 18.75, 9.375 and 4,6875 $\mu\textrm{g}$/plate), no increase in the number of revertant colonies in each strain was observed, irrespective of treatment with the metabolic activation system(S-9 mix) The chromosome aberration test was carried out using CHL cells, cell line from chinese hamster lung. With 4 doses(75, 37.5, 18.75 and 9.375 $\mu\textrm{g}$/ml) of rhG-/CSF the cells were treated for 24 or 48 hours in the direct method or for 6 hours followed by 18 hour-expression time in the metabolic activation method. Results of the study showed, by the direct method or metabolic activation method, no trend toward increase in the number of aberrant metaphase. The micronucleus test was carried out using ICR mice at the age of 8 weeks. Three doses(862.5, 1725 and 3450 $\mu\textrm{g}$/kg) of DA-3030 were admintstered intraperitoneally with single shot and bone marrow cells were sampled at 24 hours after administration. Neither the number of polychromatic erythrocytes with micronuclei nor the ratio of normochromatic erythrocytes to polychromatic erythrocytes increased singinficantly in each dose, compared with a vehicle control. These results indicate that rhG-CSF has not mutagenic potential under the condiions.

• Journal of Physiology & Pathology in Korean Medicine
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• v.34 no.6
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• pp.355-361
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• 2020

This study was designed to assess the toxicity of capsaicin-containing (CP) pharmacopunture using an in vitro chromosomal aberrations in Chinese hamster lung (CHL/IU) cells. In order to determine the high dose level in the main study of this study, a dose range finding study was conducted first. The high dose was selected at 10.0% of CP pharmacopuncture extract, and then diluted sequentially to produce lower dose levels of 5.00, 2.50, 1.25, 0.625 and 0.313% by applying a geometric ratio of 2. As a result, the cytotoxicity and precipitation of the CP pharmacopuncture as a test substance were not evident at any dose level during short-time treatment with and without metabolic activation and continuous treatment without metabolic activation. Therefore, the dose levels for this study were chosen as 10.0, 5.0, and 2.5%., and the treatment volume was 1.3 mL. In addition, negative and positive controls were set. In main study, the frequency of cells with chromosome aberrations in CP treated groups was less than 5% in short-time treatment with and without metabolic activation and continuous treatment without metabolic activation. In addition, there was no statistically significant difference when compared to the negative control group. The frequency of cells with structural chromosomal aberrations in the positive control group was more than 10% compared to the negative control group, and it increased statistically significantly. In conclusion, under the conditions of this study, CP pharmacopuncture did not show the possibility of causing chromosome aberrations.

• Journal of Korean Medicine Rehabilitation
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• v.31 no.1
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• pp.59-79
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• 2021

Objectives This study was performed to observe the genotoxic effect of the ChondroT. Methods To evaluate the genotoxicity of ChondroT, an experiment of bacterial reverse mutation test, in vitro mammalian chromosomal aberration test and mammalian erythrocyte micronucleus test in mouse was conducted. Results TA98, TA100 and TA1537 strains in the absence of metabolic activation system (S9 mix), the number of revertant colonies being greater than 2-fold of the respective negative control value. Both in -S9 mix and +S9 mix, the frequencies of aberration cells with structural aberration and numerical aberrations of chromosome were less than 5%. There was no increase of polychromatic erythrocyte with one or more micronuclei at any dose of test substance compared to the negative control group (p<0.05). Conclusions In TA98, TA100 and TA1537 strains in the absence of metabolic activation system (S9 mix), the number of revertant colonies was greater than 2-fold of the respective negative control value, showing positive results. ChondroT was considered to be non-clastogenic to Chinese hamster lung (CHL/IU) cells under the present experimental condition. and ChondroT was determined not to induce an increased frequency of micronuclei in the bone marrow cells of male ICR mice under the present experimental condition.

• Korean journal of applied entomology
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• v.15 no.4 s.29
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• pp.199-204
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• 1976

Canna nosaic X bodies, which do not exist in tissues of the healthy plant and are originating in cells of virus infected Canna (Canna generalis BAILEY) with mosaic symptom, are easily observed under microscope through application of vital staining for 2-3 minutes with $1\%$ eosin of $H_2O$ solution added with slight amount of $CH_3COOH$ and distinguishing with N/5HCl followed by washing to inspect. The result of this experiment is summarized as following: 1) The X bodies are observed not only in epiermal cells of leaf of the mosaic virus infected Canna but in those of leaf sheath, stem, and root also, and it is expected that the X bodies are to exist in the flower cells of the disease infected Canna which were missed in this experiment. 2) Shape and nature of X bodies are not constant; in early stage of the disease development, the X bodies have equal contents and vague contour with their small size and round shape, but along with progress of the disease development they attain granular contents and clear contour with their increasing sige and defining shape in cytoplasm. In case of same individual pant, fully developed X. bodies. are increasing in cytoplasm in propoition to severity of mosaic and nettling of the diseased leaf. 3) The staining character of X bodies to eosin is more dense than that of nuclei; Xbodies are stained light red or red while nuclei are stained yellowish brown or light red. 1) It is assumed to be a result of cytoplasmic concentration around nucleus that X bodies are usunlly developed adjacent to nucleus and they are considered to be a cytoplasmic prodct. 5) Thus, I confirm that X bodies originsting in canna plant cells infected with mosaic virus aye multipling in the alive cells.

• Korean Journal of Ecology and Environment
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• v.39 no.3 s.117
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• pp.378-389
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• 2006

Seasonal changes of biomass and cell size of bacteria and protozoa, and factors affecting their distribution in Lake Paldang and Kyungan Stream were analyzed from April to December, 2005. Bacterial abundance at Paldang Dam and Kyungan Stream was similar, but it did not much increase during hot summer period. Protozoan carbon biomass was much greater at Kyungan Stream compared to Paldang Dam. HNAN generally accounted for the majority of total protozoan biomass, but ciliates made up the highest proportion in April and November at Paldang Dam and June at both sites. PNAN showed low biomass at both sites, but it was high during spring and fall season. Small-sized HNAN ($3{\sim}7\;{\mu}m$) numerically predominated the protozoan community at both sites. Average cell size of HNAN was bigger at Kyungan Stream where nutrients concentration was much higher than Paldang Dam. Average cell size of ciliates varied seasonally; it was relatively small during the summer. HNAN biomass significantly correlated with Chl-a concentration and ciliates biomass at Paldang Dam, indicating that HNAN increase might link to the ciliates increase. At Kyungan Stream, HNAN biomass showed a significant relationship with PNAN biomass, and Chl-a concentration was closely related with both of HNAN and PNAN biomass. Ciliate biomass showed significant relationship with nutrient (TN, TP) and particulate matter (SS) only at Kyungan Stream. At both sites, protozoan biomass was significantly correlated with bacterial biomass, and ciliates were additionally related flagellates. High biomass of microbial components and the close relationships among them suggest that the energy transfer through the microbial loop may important in the plankton food web of Lake Paldang ecosystem.

• Journal of the korean academy of Pediatric Dentistry
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• v.32 no.2
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• pp.344-356
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• 2005

There are normal inhabitants doing medically useful functions in the body. There are many kinds of bacteria performing specific functions in the oral cavity. Two strains of lactic acid bacteria were isolated from inhabitants of caries-free children's oral cavity, which inhibited the formation of artificial plaque by Streptococcus mutans and the production of volatile sulfur compounds by anaerobic bacteria. The isolates were identified by the test using API 50 CHL medium kit and 16S rDNA partial sequencing. 1. Two isolates were Gram-positive bacilli and produced hydrogen peroxide. 2. When Streptococcus mutans was cultured in the media, the mean weight of formed artificial plaque on the orthodontic wires was $124.4{\pm}30.4\;mg$, whereas being reduced to $5.2{\pm}2.0mg$ and $10.6{\pm}6.6mg$ in the media cultured with Streptococcus mutans and each isolate, respectively (p<0.05) 3. The number of viable cells of Streptococcus mutans was $3.4{\times}10^9$ per ml in the cultured solution, whereas those of Streptococcus mutans in the combined culture with each of isolates were $4.6{\times}10^8\;and\;2.4{\times}10^8$ per ml. 4. The optical density was 1.286 in the supernatant of Fusobacterium nucleatum after vortexing for 30minutes, whereas in the supernatant of combined Fusobacterium nucleatum and each isolate, they were reduced to 0.628 and 0.497, which the percentages of coaggregation between them were 29.4% and 57.8%, respectively 5. The optical density of Fusobacterium nucleatum precipitate was 1.794 in the culture media containing cysteine and $FeSO_4$, being reduced to 1.144 and 0.915 in the coaggregated precipitates of Fusobacterium nucleatum and each isolate. The optical density of Porphyromonas gingivalis precipitate was 1.932 in the culture media, being reduced to 1.170 and 1.266 in the coaggregated precipitates of Porphyromonas gingivalis and each isolate. 6. When two isolates were tested with API 50 CHL medium kit, those were identified Lactobaciallius salivarius and Lactobaciallius delbrueckii subsp. lactis. 7. The similarity values of 16S rDNA sequence between each of isolates and Lactobaciallius salivarius subsp. salicinius were 99.60% and 99.73%, respectively, meaning that isolates were Lactobaciallius salivarius subsp. salicinius. These results indicated that two strains isolated from caries-free children's saliva, which inhibited the formation of artificial plaque and the production of volatile sulfur compounds, were identified as Lactobaciallius salivarius subsp. salicinius.

• Korean Journal of Ecology and Environment
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• v.36 no.2 s.103
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• pp.181-190
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• 2003

An investigation was conducted on the aquatic environment of the Okchon Stream watershed six times from May to September 2002. The results of investigation revealed that variation of environmental factors were quite significant for each stream and reach, showing a significant difference between running water and stagnant water. Aquatic nutrients were relatively low in the upstream, gradually increasing as the influx of treated wastewater into the stream increased. This suggests that the point source definitely affected the nutrient content of the stream. In particular, the variations of SRP and $NH_4$ were very distinct in the watershed compared to other nutrients. Thus, it can be considered as a major factor in evaluating the effect of treated wastewater. Immediately after the influx of treated waste-water, the average content of SRP rose to 919.3 ${\mu}g$ P/l. This was a very effective level in the watershed, suggesting that the percentage of the nutrients in the water was controlled by the content of P. The constant supply of treated wastewater was found to be a critical factor in triggering the increase in chl-a in the embayment of the stream. With the proliferation of the blue-green algae, the content of chl- a ranged 234.5${\sim}$1,692.2 ${\mu}g/l$. The maximum standing crops exceeded $1.0{\times}10^6$ cells/ml in August, which was more than 200 times the level for red tide in the freshwater. This result was well reflected in other environmental factors, with 100% of AFDM/TSS reflecting the severity of water pollution by algae. Therefore, the reduction of P and N con-tents in the treated wastewater is critical in improving the aquatic environment of the stream as well as water quality management for the reservoir.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.1
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• pp.45-55
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• 1998

Phytoplankton community and primary productivity have been investigated in a fall season in the southern coastal waters of the last Sea, Korea. A strong thermocline formed at the 20\~60\;m$ layer and a cold water mass also existed in the bottom around Yong-il Bay. The offshore of the surveyed area was likely to be influenced by relatively warmer water, whereas the inshore represented Higher primary productivity with lower water temperature and lower salinity. A total of 133 species of phytoplankton occurred, representing 107 spp. of diatom, 23 spp. of dinoflagellate 3 spp. of silicoflagellate. Skeletonema costatum and Asterionellepsis glacialis were most predominant with more than $30\%$ dominance ratio, while Leptocylindrus danicus was also dominant at all transect lines. Standing crops of phytoplankton ranged from $2.7{\times}10^3\;to\;141.6{\times}10^3\;cell^{\ell-1}$. Chlorophyll a concentration varied with stations and layers, but the $30\~50$ m layer showed maximun with about $1.18{\mu}g{\ell}^{-1}$ rather than at the surface layer. It is believed that the maximun in standing crops and chlorophyll of phytoplankton formed at the $20\~50$ m layer above the thermocline during the survey. Phytoplankton primary productivity ranged from 0.32 to 3.04 mgC $m^{-3}\;hr^{-1}$, showing higher at the inshore than at the offshore. The range of integrated primary productivity was $263.3\~1085.5 mgC\;m^{-2}\;day^{-1}$ for the euphotic layer. Photosysthesis rates varied with the range from 0.76 to 8.04 mgC mgChl $\alpha^{-1}\;hr^{-1}$. Phytoplankton photosynthesis at the inshore was saturated at lower irradiance ($15\~35\%$ of surface) and showed higher efficiency, Thus, it revealed that the phytoplankton community probably adapted to the middle of euphotic layer because the depth of mixing layer became thinner due to the formation of thermocline.