• Yonsei Medical Journal
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• 제59권9호
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• pp.1138-1142
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• 2018

This study aimed to analyze the impact of sleep deprivation (SD) on cardiac, hemodynamic, and endothelial parameters and to determine whether these are sustained with increased periods of SD. The study included 60 healthy men (mean: age $31.2{\pm}6.3years$; body mass index $24.6{\pm}2.6kg/m^2$). Hemodynamic parameters, parameters of myocardial contractility, spectral analysis of heart rate (HR) and blood pressure (BP) variability, and the sensitivity of arterial baroreflex function were evaluated. Biochemical tests were performed to assess L-arginine (L-Arg) and asymmetric dimethylarginine (ADMA) levels in reflection of endothelial nitric oxide synthase ability. Measurements of cardiovascular system parameters were obtained at 9 a.m. (baseline) on the first day of the study and 9 a.m. (24-h SD), 1 p.m. (28-h SD), and 5 p.m. (32-h SD) on the second day. Blood samples for evaluating biochemical parameters were obtained at baseline and after 24-h SD. ANOVA Friedman's test revealed a significant effect for time in relation to HR (${\chi}^2=26.04$, df=5, p=0.000), systolic BP (${\chi}^2=35.98$, df=5, p=0.000), diastolic BP (${\chi}^2=18.01$, df=5, p=0.003), and mean BP (${\chi}^2=28.32$, df=5, p=0.000). L-Arg and ADMA levels changed from $78.2{\pm}12.9$ and $0.3{\pm}0.1$ at baseline to $68.8{\pm}10.2$ and $0.4{\pm}0.1$ after 24-hr SD, respectively (p=0.001, p=0.004). SD in healthy men is associated with increases in BP, which appear to occur after 24 hours of SD and are maintained over increasing periods of SD. The observed hemodynamic changes may have resulted due to disordered vascular endothelial function, as reflected in alterations in L-Arg and ADMA levels.

• 대한의용생체공학회:의공학회지
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• 제39권4호
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• pp.168-174
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• 2018

Adipocytes affect obesity through the regulation of lipid metabolism. Physical loading is an important regulator of fat tissue. There are ongoing in vitro studies inducing mechanotransduction on 3T3-L1 preadipocytes with mechanical stimulus in order to treat obesity by inhibiting adipogenesis and provoking cell death. In this study, our goal was to suggest a new therapy for obesity by investigating whether fluid shear stress (FSS) changes transcription factors on 3T3-L1 related with adipogenesis and cell death. FSS loading was applied to 3T3-L1 preadipocytes at 1Pa and 1Hz. After loading, bright field images were taken and an immunofluorescence assay was conducted to observe actin stress fiber formation. Western blot analysis was conducted to identify the activation of the ERK pathway as well as the adipogenic factors, which including C/EBPs and $PPAR{\gamma}$. The expression of osteopontin, a protein related to inflammation in adipose tissue, and cell death related factors, Bax, Bcl-2, and Beclin, were also measured. Results showed that FSS stimulated the formation of actin stress fibers in 3T3-L1 and also that the activation of C/EBPs decreased significantly when compared with the control group. $PPAR{\gamma}$ activation in the 2 hour FSS group was lower than the 1 hour FSS group, which implied that the results were time dependent. Additionally, there were no differences in the expression of cell death factors after FSS loading. In summary, similar to other fibroblasts, the formation of actin stress fibers induced by mechanotransduction may affect the differentiation of 3T3-L1, leading to inhibition of adipogenesis and inflammation.

• Journal of Pharmaceutical Investigation
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• 제32권1호
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• pp.7-11
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• 2002

The purpose of this study was to develop a new $^{l3}C-urea-containing$ capsule for diagnosis of H. pylori. The urea-containing capsules were prepared with various diluents such as polyethylene glycol (PEG), microcrystalline cellulose, sodium lauryl sulfate and citric acid. The dissolution test, $^{l3}C-urea$ breath test and stability test were then performed on the capsules. Microcrystalline cellulose and sodium lauryl sulfate retarded the initial dissolution rates of urea. However, PEG increased the initial dissolution rates of urea. Furthermore, two formulae composed of PEG, [$^{l3}C-urea/PEG$ (38/1.9 mg/cap)] and [$^{l3}C-urea/PEG/citric$ acid (38/1.9/1.9 mg/cap)] had the maximum DOB value, about 16 at 20 mim, while the formula composed of only 38 mg $^{l3}C-urea$ had the maximum DOB value at 30 min. The results indicated that PEG improved the, sensitivity of $^{l3}C-urea$ in the human volunteers. The capsule [$^{l3}C-urea/PEG$ (38/1.9 mg/cap)] was stable for at least six months in 25 and $37^{\circ}C$. Thus, a PEG-containing capsule, [$^{l3}C-urea/PEG$ (38/1.9 mg/cap)] would be a more economical, sensitive and stable preparation for diagnosis of H. pylori.

• Plant Resources
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• 제6권3호
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• pp.233-241
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• 2003

The shoot tip and young leaf of Rhodiola sachalinensis were cultured to invest the plant growth regulator condition for callus induction, shoot and root regeneration. When the shoot tip was sterilized in 2.0% of NaOCl for 20min., the contamination rate was the lowest. And the survival rate of the culture material was good in carbenicillin 500mg/L treatment group. Callus was obtained from shoot tip and young leaf segments. NAA 0.1-1.0mg/L and 2,4-D 0.1-0.5mg/L alone treatment were shown to have a good response on callus induction from shoot tip culture. In the case of young leaf culture, NAA and 2,4-D 0.1-0.5mg/L alone treatment were good in callus induction. In culturing shoot tip NAA 0.5mg/L and BA 0.5mg/L, NAA1.0mg/L and BA 0.lmg/L combination treatment was good in shoot regeneration. The regenerated shoots were rooted on MS medium supplemented with NAA and BA combination treatment. Especially, NAA 1.0mg/L and BA 0.1mg/L combination treatment was effective for root regeneration.

• 원예과학기술지
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• 제29권5호
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• pp.467-473
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• 2011

Flavonoid are large group of the polyphenolic compounds which are distinguished by an aromatic or phenolic ring structure and the phenolic compounds are induced by microbial infection, ultraviolet radiation, temperature and chemical stress. They are known for their antioxidant activity, anti-allergic, anti-inflammatory, anti-microbial and anti-cancer activities. In this study, changes in flavonoid content were investigated using heterologous chalcone isomerase (CHI) expression system. Also, phenolic compounds level was measured to examine the relation between flavonoids and phenols contents. Explants of lettuce (Lactuca sativa L.) were transformed with Agrobacterium tumefaciens LBA 4404 strain containing pFLH-CHI (derived from pPZP2Ha3) vector constructed with CHI gene from Brassica rapa. The putative transgenic plants were confirmed by genomic DNA PCR analysis. Also the transcription levels of the gene were analyzed by semi-quantitative RT-PCR with gene specific primers. The total flavonoid contents were increased at $T_0$ and $T_1$ generations over 1.4 and 4.0 fold, respectively. Total phenol contents also increased at $T_1$ generation. These results indicate that CHI gene plays an important role to regulate the accumulation of flavonoids and its component changes.

• 한국전산구조공학회논문집
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• 제33권3호
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• pp.145-152
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• 2020

본 논문에서는 유한요소해석을 이용하여 흉요추 후방 고정술의 고정분절 변화에 따른 척추 안정성을 평가하였다. 이를 위해 추간판, 인대, 추간관절(Facet joint) 등을 포함한 정상 흉요추(T10-L4)의 유한요소모델을 구축하였으며, 문헌으로 보고된 재료물성치를 부여하였다. 한편, L1을 병변 부위로 가정하였으며, L1-L2, T12-L2, T12-L1-L2 총 3가지 종류의 후방 고정술을 흉요추 유한요소모델에 구현하고 전방 굽힘, 후방 굽힘, 측면 굽힘, 축 회전의 하중 조건을 부여하였다. 시리즈 유한요소해석을 통해 고정분절에 따른 척추경 나사못, 척추골, 추간판의 변형량, 등가 응력, 운동 범위, 모멘트를 계산하였으며, 그 결과를 바탕으로 척추 안정성을 평가하였다.

• 한국작물학회지
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• 제61권1호
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• pp.33-40
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• 2016

This study is focused on the evaluation of growth parameters, total polyphenol content (TPC), chlorophyll content as well as the DPPH (1,1-diphenyl-2-picryhydrazyl) free radical scavenging activity of young barley seedling (YBS) affected by elicitation. Salicylic acid (SA), methyl jasmonate (MJ), amino acid liquid fertilizer (ALF) and microbial metabolism activator (MMA) were used. Elicitation was conducted for two times and various concentrations were used in this study. The result revealed that, MJ 1 ml/L treated-YBS gave the longest seedling length of 1.33 cm, followed by the ones treated with SA 1.38 mg/L and ALF 2 ml/L, respectively. ALF 3 ml/L treatment gave the highest fresh weight of 10 seedlings, followed by MJ 5 ml/L and SA 13.8 mg/L treatment with 1.56 g, 1.55 g and 1.53 g respectively. SA 138.12 mg/L elicitor treated-YBS gave the highest Chl a, Chl b content of $8.57{\mu}g/mg$ and $3.83{\mu}g/mg$, respectively while the highest carotenoid content was found in MJ ml/L treatment with $1.62{\mu}g/mg$. Among elicitor treated-YBS, SA showed better TPC. The highest TPC was found in SA 1.38 mg/L treatment with 18.82 mg/g TAE. Likewise, SA 1.38 mg/L showed the highest DPPH free radical scavenging activity among all the treatments. However, the lowest TPC was found in ALF 1ml/L treated-YBS with 9.46 mg/g TAE, which was even lower than the control (14.31 mg/g TAE).

• 물과 미래
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• 제19권4호
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• pp.355-364
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• 1986

본 연구는 해빈변형과정에 침식과 퇴적사이의 상사성을 현지 및 실험실에서 결정하려고 수행되었다. 얻어진 결과는 다음과 같았다. (1) Horikawa 식의 이론을 적용하여 연구한바 침식과 퇴적사이의 상사성을 C값만으로 결정하기는 곤란함을 알았다. (2) $H_//L_0$식만의 값으로 상사성을 결정하기는 곤란하였다. (3) $H_//L_0$와 C, $H_//L_0$와 $N_{sr}$의 무차원값을 현지 및 실험실에서 관찰한바에 의하면 $H_//L_0$가 0.024에서 침식, 퇴적이 구분되어 상사가 이루어졌다. (4) 파형경사를 알면 침식, 퇴적을 구분하는데 크게 도움이 될 것이다.

• 한국전자파학회논문지
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• 제9권6호
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• pp.813-823
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• 1998

본 논문은 비분리형 비등방성 완전정합충(UAPML)을 이용하여 이동통신용 원형편파 등각 마이크로스트립 패치 안테나를 해석하였다 또한 3차원 U따까1L에 대한 모서리 및 모퉁이 부분에 대하여 특별히 다루었다. 특히 동축 여기선을 갖는 마이크로스트립 패치 안테나를 해석하기 위해서 Mur의 1차 흡수경계조건을 혼합 적용하였다 결과적으로 본 논문은 UAPML법이 모서리 및 모퉁이 부분에서 수렴함은 물론 Mur의 1차 흡수 경계조건과 혼합하여 홈수경계조건으로서 충분히 사용 가능함을 제시한다. 수치해석 결과는 이동통신 대역인 L밴드 및 C 밴드에서 중심주파수 1.575 GHz, 1.778 GHz 그리고 4.8 GHz를 갖는 단일 및 병렬 패치에 대한 전자장의 $E_z$및 $H\chi$에 대한 시간응답, 동축선의 입력임피던스 및 마이크로 스트립 패치의 복사특성을 해석 하였다 본 논문의 해석 결과는 2차 분산 경계조건(SDBC) Mur의 1차 흡수경계조건을 혼합한 수치해석 방법 그리고 순수 Mur의 1차 흡수경계조건과 비교하였다. 따라서 본 논문에서 제시된 해석방법이 합당함을 입 증할 수 있다.

• 한국식품저장유통학회지
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• 제14권2호
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• pp.188-193
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• 2007

하향주의 제조에 이용되는 누룩으로부터 젖산균을 분리하고, 배양학적 특성과 생리적 특성을 검토하였다. 분리된 총 11균주를 이용하여 알코올 10%와 pH 4.0에서 생육이 억제되는 양조학적으로 적합한 5균주의 젖산균을 분리하였고, 16S rRNA의 염기서열을 분석하여 동정한 결과 Lactobacillus plantarum 1균주, Lactobacillus sakei 1균주 및 Lactobacillus curvatus 3균주로 나타났으며 모두 젖산 간균으로 확인되었고, 이들을 각각 L. plantarum L-3, L. sakei L-10 그리고 L. curvantus L-8, 11, 12로 명명하였다. L plantarum L-3이 배양 초기 pH 4.0일 때 0.78%의 산도를 나타내어 나머지 4균주에 비해 약 10배정도 높은 산을 생성하였다. 분리된 5균주는 모두 $30^{\circ}C$에서 가장 산생성능이 우수하였고, L. sakei L-10을 제외한 모든 균주는 $45^{\circ}C$에서 생육이 정지되었으나, L. sakei L-10은 $40^{\circ}C$에서 생육이 저해되었다. 그리고 에탄을 농도가 6%일 때 산의 생성이 급격히 감소되었으며 L. curvatus L-8, 11, 12는 8% 에탄올에서 거의 생육이 이루어지지 않아 에탄올에 대한 내성이 낮았으며, L. plantarum L-3과 L sakei L-10은 10% 에탄올에서 생육이 정지되었다.