• The Korean Journal of Zoology
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• v.9 no.1
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• pp.7-15
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• 1966

In a previous paper, the dirunal and monthly changes of the tidal flat temperature and chlorinity were observed. In this paper the resistance of the young short necked clam in various growth stages to the temperature, to the chlorinity and to the exposure were studies. The young clam used were in early (E), medium (M) and late (L) young stages whose shell lengths were 2.0-3.5mm, 9.0-11.0mm. and 14.0-16.0mm., respectively. The results were : 1. At various sea water temperatures , the chlorinity resistance of the young clam was directly proportional to the shell length. 2. When both inadequate sea water temperature and abnormal chlorinity are simultaneously applied, the resistance of these young clams was more markedly reduced than the case of applying either one of these conditions. 3. In clams of M and L, no lethal effect were observed when daily immersion of four to eight hours for a week into the sea water of any concentration of chloriity at 26-34$^{\circ}C$, whereas in E, 37-90% of mortalities were obtained by immersion of eight hours daily into both the fresh water and the sea water of higher chloriniites (more than 23.5$\textperthousand$ Cl) at the same temperature above. 4. The lower critical thermal maximum for lethal to the young clam was 38$^{\circ}C$. With four hours immersion daily at the water temperature of 38$^{\circ}C$, the mortalities of E, M and L to the lower chlorinities (less than 6.7$\textperthousand$Cl) were 100, 70-100 and 27-37% respectively ; to the higher chlorinities (more than 23.5$\textperthousand$Cl) 10-70, 10-37 and 3 % respectively ; to the normal range of chlorinities (13.4-16.8$\textperthousand$Cl) 0-13, 3 and 0 % respectively. 5. No lethal effects were observed in E and M clams immersed continuously for seven days in sea water with chlornities of 7.2 -21.7$\textperthousand$Cl at 18-24$^{\circ}C$, while notable mortalities were observed in E which had been kept at lower (less than 4.8$\textperthousand$ Cl) and higher (more than 24.1$\textperthousand$ Cl) chlorinites. 6. Although the resistance of the young clam to the chlorinity may have to be related closely to the life history of the clam prior subject to the experiment, the adapted chlorinity range was 7.2-19.3$\textperthousand$ Cl and the optimum range was 13.4-16.8$\textperthousand$Cl. 7. Remarkable lethal effects were observed for the E and M clams to the exposure temperature of 38$^{\circ}C$ whereas the L and had no such fatal results.

• Molecules and Cells
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• v.40 no.8
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• pp.567-576
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• 2017

The $Na^+/H^+$ exchanger is responsible for maintaining the acidic tumor microenvironment through its promotion of the reabsorption of extracellular $Na^+$ and the extrusion of intracellular $H^+$. The resultant increase in the extracellular acidity contributes to the chemoresistance of malignant tumors. In this study, the chemosensitizing effects of cariporide, a potent $Na^+/H^+-exchange$ inhibitor, were evaluated in human malignant mesothelioma H-2452 cells preadapted with lactic acid. A higher basal level of phosphorylated (p)-AKT protein was found in the acid-tolerable H-2452AcT cells compared with their parental acid-sensitive H-2452 cells. When introduced in H-2452AcT cells with a concentration that shows only a slight toxicity in H-2452 cells, cariporide exhibited growth-suppressive and apoptosis-promoting activities, as demonstrated by an increase in the cells with pyknotic and fragmented nuclei, annexin V-PE(+) staining, a $sub-G_0/G_1$ peak, and a $G_2/M$ phase-transition delay in the cell cycle. Preceding these changes, a cariporide-induced p-AKT down-regulation, a p53 up-regulation, an ROS accumulation, and the depolarization of the mitochondrial-membrane potential were observed. A pretreatment with the phosphatidylinositol-3-kinase (PI3K) inhibitor LY294002 markedly augmented the DNA damage caused by the cariporide, as indicated by a much greater extent of comet tails and a tail moment with increased levels of the p-histone H2A.X, $p-ATM^{Ser1981}$, $p-ATR^{Ser428}$, $p-CHK1^{Ser345}$, and $p-CHK2^{Thr68}$, as well as a series of pro-apoptotic events. The data suggest that an inhibition of the PI3K/AKT signaling is necessary to enhance the cytotoxicity toward the acidtolerable H-2452AcT cells, and it underlines the significance of proton-pump targeting as a potential therapeutic strategy to overcome the acidic-microenvironment-associated chemotherapeutic resistance.

• Journal of the Korean Society of Physical Medicine
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• v.9 no.2
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• pp.151-159
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• 2014

PURPOSE: The purpose of this study was to evaluate whether light-emitting diodes (LED) irradiation could be effective in a noninvasive, therapeutic device for the treatment of osteoarthritis(OA). METHODS: Twenty-four male Sprague-Dawley rats were divided into four groups: Vehicle control (saline); monosodium iodoacetate-injection (MIA); LED irradiation after MIA injection (MIA-LED); indomethacin-treatment after MIA injection (MIA-IMT). OA was induced by intra-articular injection of 3 mg MIA through the patellar ligament of the right knee. Vehicle control rats were injected with an equivalent volume of saline. The LED was irradiated for 15 min/day for a week after 7 days of MIA treatment. To compare with the effect of LED irradiation, the indomethacin was administrated 20 mg/kg twice a week orally after 7 days of MIA treatment. Knee joints were removed and fixed overnight in 10% neutral buffered formalin and decalcified by EDTA for 2 week before being embedded in paraffin. The assessment of OA induction were monitored by knee movement and radiographic finding. Histologic analysis were performed following staining with hematoxylin and eosin, safranin O-fast green, or toluidine blue, picrosirius red, and histologic changes were scored according to a modified Mankin system. Apoptotic cell in tissue sections was detected using TUNEL method. RESULTS: Radiographic examination could not show the differences between the MIA-treated and the MIA-LED-treated rats. In the histologic analysis, however, LED irradiation prevented cartilage damage and subchondral bone destruction, and significantly reduced mononuclear inflammatory cell infiltration and pannus formation. LED irradiation also reduced apoptosis of cartilage cells, but it prevented apoptosis of infiltrated inflammatory cells in synovium. In addition, LED irradiation showed an increase of collagen production in the meniscus. CONCLUSION: These results suggest that the 840 nm LED irradiation would be a suitable non-thermal phototherapy for the treatment of OA, as a cartilage protection and anti-inflammatory modality.

• Journal of Ginseng Research
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• v.37 no.4
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• pp.425-434
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• 2013

UV irradiation is the main factor contributing to skin damages that are associated with an excessive production of matrix-degrading metalloproteinase (MMP)-1 and a deficient expression of collagens. To date, red ginseng has been revealed to possess many biomedical effects, such as anti-aging, anti-oxidation, and anti-inflammatory. In this study, we prepared the Korean Red Ginseng extracts treated with enzyme (KRGE) and investigated the effects of dietary KRGE on the formation of wrinkles generated by UVB irradiation in hairless mice. It was found that KRGE inhibited the UVB-induced formation of wrinkles, epidermal thickness, and skin dryness in hairless mice. Further results also showed that KRGE attenuated UVB-induced MMP-${\beta}$1 level, while accelerated procollagen type I, transforming growth factor-${\beta}$1 secretion. Interestingly, the expression of profilaggrin and filaggrin in both the epidermis and dermis were decreased due to UVB exposure and reversed by KRGE. The KRGE 0.06% was prior to KRGE 0.24%. In view of these results, which indicated that KRGE protected skin from UVB-induced photodamages, which may not only mediated by regulating of MMP-1 and procollagen type I, but also by increasing the production of profilaggrin and filaggrin. In conclusion, our results suggest that KRGE may be a promising agent for the treatment of skin photodamages. The challenge of KRGE will be expected as cosmeceuticals and nutraceuticals in order to intervene in aging-related degenerative skin changes.

• Journal of Wetlands Research
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• v.23 no.4
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• pp.296-306
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• 2021

Elasticity is a statistical technique that interprets the changing pattern of another variable according to a change in one variable as a quantitative numerical value and provides more information than correlation analysis and is widely used in climate change research. In this study the elasticity was calculated and sensitivity analysis was performed using air temperature and water quality data of the major tributaries of the Nakdong River. In addition the confidence interval for the elasticity was calculated using the T-Test and the validity of the elasticity was examined. The strength of elasticity shows high strength in the order of summer>fall>spring>winter and the direction shows regional characteristics with both negative and positive elasticity. After performing hierarchical cluster analysis on monthly observation data they were classified into 5 clusters and the characteristics of each cluster were visually analyzed using a parallel coordinate graph. The direction and intensity of the air temperature elasticity show regional characteristics due to the relatively high population density and complex influencing factors such as sewage treatment plants, small-scale livestock houses and agricultural activities. In the case of TP it shows great regional variability according to the circulation of nutrients in the ecosystem caused by algae growth and death according to temperature changes. Since the air temperature elasticity of the major tributaries of the Nakdong River is over weak and is valid at the significance level of 5%, it was analyzed that there is a change in water quality according to the air temperature change.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.14 no.2
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• pp.168-174
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• 2021

According to the World Agricultural Productivity Report, the current annual average growth rate of agriculture is 1.63%, which is lower than 1.73% to support the world's 10 billion people, which is growing by 2050. The demand for food, feed, and bioenergy is not growing enough to continue to meet the demand, and it is predicting a future food shortage. The purpose of this study was to create a regional irrigation control model for the purpose of reducing the production cost of crops, increasing production, and improving quality, and presenting a model that can give advice to farmers who start farming in the region. The irrigation control modeling presented in this study means to represent the change of medium weight·supply liquid·drainage amount due to changes in the root zone environment according to the passage of time and climate in a graph model. For water control modeling, we collected data on the change in the amount of the root zone environment and the weight of the badge·supply amount·drainage amount from March to June in Nonsan, Buyeo, and Yesan regions in Chungnam Province through the measuring device of the Ministry of Environment in the root region. We set up the parameters for derivation and derived an irrigation control model that can confirm the change in weight·supply liquid·drainage amount over time through the parameters.

• Journal of Mushroom
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• v.18 no.4
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• pp.297-302
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• 2020

Five varieties of mushrooms, including Sae-do, were examined using farm field tests to determine their morphological characteristics and quality by performing harvest flush. Considering the cultivation characteristics of the varieties studied, the parts related to mushroom generation and production showed almost similar characteristics. Overall, the mycelial forces were similar; however, in the evaluation of plantation tests performed by farmers, "saedo" was found to be the strongest and "saehan" was considered to be the weakest. Weights of fruiting bodies were related more for compost status and regimen of use than for breeding, and it was difficult to distinguish between the weights of fruiting bodies of the varieties studied. Regardless of the variety, pileus diameter, pileus thickness, and stem length of Dalseong-gun, Yongin-si, and Boryeong-si decreased as the harvest flush progressed. However, stem thickness tended to increase in the second and third flush compared to that in the first flush, regardless of the variety. However, the Gyeongju-si farms could not identify any trends attributable to varieties and harvest flushes. The pileus size and stem length of the fruiting bodies of varieties from Buyeo farms decreased with the progression of the harvest flush. However, pileus thickness and stem thickness did not show any specific tendency of growth among the varieties studied. The differences in the morphological characteristics of mushroom varieties in each harvest cycle are expected to be caused by differences in the supply of nutrients in the medium, the quality of the compost used, and the cultivation environment rather than the variety.

• Journal of Chest Surgery
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• v.56 no.3
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• pp.155-161
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• 2023

Background: Surgical closure of an atrial septal defect (ASD) is infrequently indicated during infancy. We evaluated the clinical characteristics and outcomes of patients who underwent surgical ASD closure during infancy. Methods: A single-center retrospective review was performed for 39 patients (19 males) who underwent surgical ASD closure during infancy between 1993 and 2020. The median body weight percentile at the time of operation was 9.3. Results: During a median follow-up of 60.9 months, 4 late deaths occurred due to chronic respiratory failure. A preoperative history of bronchopulmonary dysplasia (BPD) was the only risk factor for late mortality identified in Cox regression (hazard ratio, 3.54; 95% confidence interval [CI], 1.75-163.04; p=0.015). The 5-year survival rate was significantly lower in patients with preoperative history of BPD (97.0% vs. 50.0%, p<0.001) and preoperative ventilatory support (97.1% vs. 40.4%, p<0.001). There were significant postoperative increases in left ventricular end-diastolic (p=0.017), end-systolic (p=0.014), and stroke volume (p=0.013) indices. A generalized estimated equation model showed significantly better postoperative improvement in body weight percentiles in patients with lower weight percentiles at the time of operation (<10th percentile, p=0.01) and larger indexed ASD diameter (≥45 mm/m², p=0.025). Conclusion: Patients with ASD necessitating surgical closure during infancy are extremely small preoperatively and remain small even after surgical closure. However, postoperative somatic growth was more prominent in smaller patients with larger defects, which may be attributable to an increase in postoperative cardiac output due to changes in ventricular septal configuration. The benefits of ASD closure in patients with BPD are undetermined.

• Korean Journal of Environment and Ecology
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• v.36 no.6
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• pp.662-675
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• 2022

This study was intended to investigate and analyze the current status of the plant community structure in order to provide basic data for the systematic conservation and management of the Buxus sinica area appearing on the ridgeline connecting the Gyeokjabong(Mt.) and Keungiljae, on Bogildo Island. In consideration of location characteristics such as density, topography, and direction of B. sinica appearing in the shrub layers, a total of 26 sites were set as the survey area for representative vegetation communities or areas with changes in topography. According to DCA and TWINSPAN, it was classified into 6 communities, namely Carpinus turczaninovii-Deciduous broad-leaved, C. turczaninovii, C. turczaninovii-B. sinica, C. turczaninovii-Evergreen broad-leaved, Q. acuta, and D. trifidus-Q. acuta. The community dominated by C. turczaninovii maintains the status quo for the present moment, however, Q. acuta will dominate the surrounding area in the long term, so Q. acuta has high succesion potential. In the case of the Q. acuta and D. trifidus-Q. acuta communities, Q. acuta, known as the climax species of warm-temperate forests, will maintain dominant status. In a case of B. sinica, the community dominated by C. turczaninovii will remain in good status due to the topographical conditions, but the community dominated by Q. acuta growth difficulties are expected due to the high coverage. In the relationship between environmental factors and vegetation distribution, analysis showed that only soil pH affect vegetation distribution. Furthermore, the soil acidity (pH) was 3.78-5.30, the electrical conductivity was 0.186-0.543 dS/m, and the organic matter content was 2.25-2.89%.

• Korean Journal of Remote Sensing
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• v.39 no.6_1
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• pp.1483-1490
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• 2023

We analyzed the potential for joint utilization of Visible Infrared Imaging Radiometer Suite (VIIRS) satellite imagery Normalized Difference Vegetation Index (NDVI) in crop assessment, considering the aging of MODerate resolution Imaging Spectroradiometer (MODIS) satellites. Over 11 years from 2012 to 2022, we examined the characteristics of NDVI changes in corn and soybean cultivation areas in Illinois, USA. VIIRS and MODIS satellite imagery NDVI exhibited a high correlation coefficient of over 0.98. However, during periods of rapid crop growth or decline, VIIRS NDVI showed values approximately 0.12 to 0.14 higher than MODIS. Estimating crop anomaly classes based on NDVI, we observed similar trends in corn and soybean crop anomaly classes in 2018 and 2019. However, in 2022, there appeared to be a significant divergence in crop anomaly classes, suggesting the need for further investigation. The correlation coefficients between MODIS and VIIRS satellite imagery NDVI and corn and soybean yields were consistently high, exceeding 0.8, indicating the potential for quantity estimation using both MODIS and VIIRS satellite imagery. Specifically, for VIIRS NDVI, excluding the increasing trend in crop quantity estimation for soybeans enhanced the correlation, and compared to MODIS, it showed a consistently high correlation with quantity from approximately 16 days earlier, indicating the potential for early estimation.