• Journal of the Korean Graphic Arts Communication Society
• /
• v.9 no.1
• /
• pp.27-44
• /
• 1991

In this research, double-layered photoconductor consist of the carrier generation layer(CGL) of ${\varepsilon}$ type copper phthalocyanine thin film by an aqueous coating method and the carrier transport layer(CGL) of polyvinyl carbazol(PVK) by spin coating. We inverstigated effect of the surfactant solution and cathod electrolysis to the crystal type of ${\varepsilon}$-CuPc in CGL with TEM, SEM and X-ray diffraction spectroscopy and studied the mechanism of an aqueous coating for the preparation of CGL. The effect of the washing of CGL about the electrophotographic characteristics of the ${\varepsilon}$-CUPC/PVK double-layered photoconductors is studied also.

• Journal of the Korean Graphic Arts Communication Society
• /
• v.9 no.1
• /
• pp.1-11
• /
• 1991

In this reseach, double-layered photoconductor consist of the carrier generation layer(CGL) of $\varepsilon$ type copper phthalocyanine thin film by an aqueous coating method and the carrier transport layer(CGL) of polyvinyl carbazol(PVK) by spin coating. We inverstigated effect of the surfactant solution and cathod electrolysis to the crystal type of $\varepsilon$-CuPc in CGL with TEM, SEM and X- ray diffraction spectroscopy and studied the mechanism of an aqueous coating for the preparation of CGL. The effect of the washing of CGL about the electrophotographic characteristics of the $\varepsilon$-CuPc/PVK doublelayered photoconductors is studied also.

• Journal of Life Science
• /
• v.21 no.1
• /
• pp.119-126
• /
• 2011

There is a growing recognition of the significance of $H_2S$ as a biological signaling molecule involved in vascular and nervous system functions. In mammals, two enzymes in the transsulfuration pathway, cystathionine ${\beta}$-synthase (CBS) and cystathionine ${\gamma}$-lyase (CGL), are believed to be chiefly responsible for $H_2S$ biogenesis. Genetic inborn error of CGL leads to human genetic disease, cystathioninuria, by accumulating cystathionine in the body. This disease is secondarily associated with a wide range of diseases including diabetes insipidus and Down's syndrome. Although the human CGL (hCGL) overexpression is essential for the investigation of its function, structure, reaction specificity, substrate specificity, and protein-protein interactions, there is no clear report concerning optimum overexpression conditions. In this study, we report a detailed analysis of the overexpression conditions of the hCGL using a bacterial system. Maximum overexpression was obtained in conditions of low culture temperature after inducer addition, performing low aeration during overexpression, and using a low concentration inducer (0.1 mM, IPTG) for induction. Expressed hCGL was purified by His-tag affinity column chromatography and confirmed by Western blot using hCGL antibody and enzyme activity analysis. We also report that the His tag with TEV site attached protein exhibits 76% activity for ${\alpha}-{\gamma}$ elimination reaction with L-cystathionine and 88% for ${\alpha}-{\beta}$ elimination reaction with L-cysteine compared to those of wild type hCGL, respectively. His tag with TEV site attached protein also exhibits a 420 nm absorption maximum, which is attributed to the binding cofactor, pyridoxal 5'-phosphate (PLP).

• Microbiology and Biotechnology Letters
• /
• v.42 no.1
• /
• pp.11-17
• /
• 2014

Selenium is an essential trace element for mammals, but it is very toxic. Therefore, the control of selenium concentrations should be precisely and effectively monitored. Selenium is naturally obtained through foods and seleno-L-methionine (LSeMet) is a major form of selenium. It has been reported that L-SeMet is only converted into Se-adenosyl-L-SeMet. However, a recent study suggested that L-SeMet was directly metabolized into methylselenol ($CH_3SeH$) in mouse liver extract by the reaction of cystathionine ${\gamma}$-lyase (CGL). The canonical reaction of CGL was known to catalyze the cleavage of L-cystathionine to L-cysteine, ${\alpha}$-ketobutyrate and $NH_3$. In the present study, we found that L-SeMet could be directly converted to $CH_3SeH$ using purified homogenous human CGL instead of mouse liver cytosol. Authentic $CH_3SeH$ was prepared by reduction of dimethyldiselenide with sodium tetrahydroborate. The gaseous product of the enzymatic reaction with L-SeMet was analyzed by GC/MS spectrometry. The GC/MS data was identical to that of authentic dinitrophenyl selenoether. We also analyzed the kinetic parameters for the formation of $CH_3SeH$ from L-SeMet by human and mouse CGL. These results suggest that human CGL is a critical enzyme which is responsible for L-SeMet metabolism.

• Corrosion Science and Technology
• /
• v.9 no.4
• /
• pp.159-163
• /
• 2010

Precise coating weight control is very important issue on quality and minimizing operating costs on continuous galvanizing line. These days, many steel making companies are having a new understanding of cost importance by rise raw material prices and customer's requirement for cost reduction. Dongbu steel also meets these situations and decided to develop the technologies. Dongbu Steel developed Integrated coating weight control system jointly with Objective Control Ltd. and installed 2CGL and 4CGL. Several technological functions were developed and realized to achieve true hands-off operation and maximum cost benefit by combining model-based preset and dynamic prediction models. We also installed it on 1 CGL on April, 2008. This paper will present the interface, functions and application result of the integrated coating weight control system including Zn saving and coating weight uniformity.

• Food Science and Preservation
• /
• v.23 no.4
• /
• pp.560-567
• /
• 2016

Sensory evaluation, in vitro antioxidant activities and main compounds of coffee water-extract, coffee liqueur (CL) and coffee-ground liqueur (CGL) were investigated to consider their industrialization. Sensory evaluation showed that all groups of CGL without 25% CGL (3 month) were relatively higher than CL groups. Total phenolic compounds and in vitro antioxidant activities such as 1,1-diphenyl-2picryl-hydrazyl (DPPH) radical scavenging activity and ferric reducing/antioxidant power (FRAP) were also performed. The group of 35% CGL had higher total phenolic compounds than others, and the result of DPPH radical scavenging activity was similar to that of total phenolic compounds. In addition, 35% CGL is comparable to the FRAP of coffee water extract (CE). Qualitative and quantitative analysis using high-performance liquid chromatography (HPLC) were performed, and chlorogenic acid as a ployphenolic compound and caffeine as a nonpolyphenolic compound were detected in all samples. Moreover, the HPLC analysis showed that CGLs contain a larger amounts of chlorogenic acid (difference of 0.3~10.5%) and also greater amounts of caffeine (difference of 10.0~18.2%) more then CE. Consequently, these results suggest that coffee-ground as coffee by-products could be used as commercially available food substances because of its physiological molecules remained.

• Proceedings of the Materials Research Society of Korea Conference
• /
• 2007.11a
• /
• pp.115.2-115.2
• /
• 2007

• Journal of the Korean institute of surface engineering
• /
• v.48 no.3
• /
• pp.105-109
• /
• 2015

We studied emission characteristics of blue fluorescent multi-tandem OLEDs using $Al/MoO_x$ as charge generation layer(CGL). Threshold voltage for 2, 3, 4, and 5 units tandem OLEDs was 8, 11, 14 and 18 V, respectively. The threshold voltage in multi-tandem OLEDs was lower than multiple of 4 V for the single OLED. Maximum current efficiency and maximum quantum efficiency of single OLED were 7.6 cd/A and 5.5%. Maximum current efficiency for 2, 3, 4, and 5 units tandem OLEDs was 22.6, 31.4, 41.2, and 46.6 cd/A, respectively. Maximum quantum efficiency for 2, 3, 4, and 5 units tandem OLEDs was 11.8, 15.8, 21.8, and 25.6%, respectively. The maximum current efficiency and maximum quantum efficiency in multi-tandem OLEDs were higher than multiple of those for the single OLED. The intensity for 508 nm peak was changed and the peak wavelength was red shift by increase of tandem unit in electroluminescent emission spectra. These phenomena can be caused by micro-cavity effect with increasing of organic layer thickness.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.31 no.1
• /
• pp.21-25
• /
• 2002

The contribution of lipid to thermal flayer generation from glucose-protein reaction was accomplished by isolating flavor compounds from casein-glucose (CG)and casein-glucose-coin oil (CGL) which were stored for 2 and 4 weeks at 6$0^{\circ}C$ and then reacted at 16$0^{\circ}C$ for 1hr. The volatiles from the reactant mixtures were isolated by a solvent extraction method with methylene chloride and analyzed by gas chromatography and gas chromatography-mass spectrometry. Pyrazine, methylpyrazine, 2,5-dimethylpyrazine, 2-dimethylpyrazine ,2-ethy-5- methyIpyrazine and 2-acetylpyrrole originated from interaction of thermal degradation of casein and lipid oxidation were identified in the CGL samples. It was also found that 3-methyl-1-butanol, 2-cyclopene-1,4-diode, heptanal, nonanal, and 2-heptanone were derived from lipid source. Two additional fatty acids, heptanoic acid and octanoic acid were also identified in the CGL samples. 5-Hydroxymethyl-2-furfural, the most abundant volatile, was responsible for the formation of sugar degradation product. The results suggested that the presence of lipid in the samples had more effect on the contribution of volatile formation of glucose-protein thermal reaction than the absence of lipid in the samples.

• Journal of the Korean Society for Precision Engineering
• /
• v.10 no.3
• /
• pp.57-68
• /
• 1993

Air Wiping technique is widely used because of easy and efficient coating control in present CGL. Coaring weight is decided by nozzle header pressure, strip line speed and distance between strip and nozzle. Coating defects are results from unbalance of these factors and coating equipment calibration inaccuracy. Therefore, this study is mainly dealing with the cause of coating defects such as edge overcoating and coating deviation. The coptimum working condition is suggested by formulated coating model using collected working data. We developed two demension analysis program for air flow in nozzle and calculated dynamic pressure and air velocity with this program. The productivity and coating guality are improved by applying the result of this reserach.