• Korean Journal of Clinical Laboratory Science
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• v.43 no.3
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• pp.124-132
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• 2011

The aim of this study was to evaluate the effects of the photosensitizer photogem with light-emitting diode (LED) on vancomycin-resistant enterococci (VRE). Two VRE strains isolated from the feces of patients. that was identificated Enterococcus faecium (vanA) and Enterococcus gallinarum (vanC1) using traditional biochemical tests and confirmed VRE genotyping from using polymerase chain reaction. In addition, three strains were used Enterococcus. faecalis CDC-286 (vanA), E. faecalis CDC-583 (vanB) and E. gallinarum CDC-42 (vanC1). To examine the antimicrobial effect of photogem mediated photodynamic therapy (PDT) against, CFU quantification and Disk diffusion antimicrobial susceptibility test were evaluated. The effects of Photodynamic therapy was not associated with genotype. Photogem mediated PDT perfectly inhibited the colony formation of E. faecalis CDC-286. The number of viable bacteria decreased greatly after PDT application with photogem $50{\mu}g/mL$ and energy density of $15J/cm^2$. The diameter of inhibition zone was increased to after PDT more than before PDT. The case of vancomycin disc on E. faecalis CDC-583 and E. galinanum-Patient were changed from resistant to intermediate resistant, from intermediate resistant to susceptable. These results demonstrate that lethal photosensitization of VRE can be achieved using photogem plus 630 nm LED irradiation.

• Bulletin of the Korean Chemical Society
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• v.30 no.6
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• pp.1313-1316
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• 2009

Cdc25 phosphatases have been considered as attractive drug targets for anticancer therapy due to the correlation of their overexpression with a wide variety of cancers. We have been able to identify five novel Cdc25 phosphatase inhibitors with micromolar activity by means of a structure-based de novo design method with a known inhibitor scaffold. Because the newly discovered inhibitors are structurally diverse and have desirable physicochemical properties as a drug candidate, they deserve further investigation as anticancer drugs. The differences in binding modes of the identified inhibitors in the active sites of Cdc25A and B are addressed in detail.

• Microbiology and Biotechnology Letters
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• v.42 no.1
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• pp.73-81
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• 2014

Salsola collina, also known as Russian thistle, is widely distributed in and around waste facilities, roadsides, and drought and semi-drought areas, and is used as a traditional folk remedy in Chinese medicine for the treatment of hypertension. In this study, we have evaluated the anti-oxidative and anti-cancer activities of the ethanol extract of S. collina Pall. (EESC), and the molecular mechanisms of its anti-cancer effects on human colon carcinoma HT29 cells. EESC exhibited anti-oxidative activity through DPPH radical scavenging capacity and showed cytotoxic activity in a dose-dependent manner in HT29 cells. After EESC treatment, HT29 cells altered their morphology, becoming smaller and irregular in shape. EESC also induced cell accumulation in the G2/M phase in a dose-dependent manner, accompanied by a decrease of cell population in the G1 phase. The G2/M arrest by EESC was associated with the increased expression of cyclin-dependent kinase (CDK) inhibitor p21 and Wee1 kinase, which phosphorylates, or inactivates, Cdc2. EESC treatment induced the phosphorylation of Cdc2 and Cdc25C, and inhibited cyclin A and Cdc25C protein expression. In addition, S arrest was induced by the highest concentration of EESC treatment, associated with a decrease of cyclin A and Cdk2 expression. These findings suggest that EESC may possess remarkable anti-oxidative activity and exert an anti-cancer effect in HT29 cells by cell cycle regulation.

• Journal of Korean Institute of Industrial Engineers
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• v.21 no.3
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• pp.313-327
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• 1995

This paper deals with ordering policies for a multi-echelon distribution system. The system we are concerned consists of one Central Distribution Center(CDC) and N nonidentical Regional Distribution Centers(RDCs) which have different demand rates, minimum filtrates, leadtimes, etc. We develop an algorithm for determining the optimal ordering policies of the CDC and the RDCs under the constraints of minimum filtrates of RDCs and maximum allowable delay of CDC.

• Proceedings of the KIEE Conference
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• 2011.07a
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• pp.386-387
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• 2011

HVDC 프로젝트마다 제어기가 달라지게 된다. 따라서 HVDC 설비를 위한 전용의 해석 모델이 존재하지 않는다. 설비의 동특성을 모의하기 위해 모델 라이브러리가 없는 상황이 도래하면, 범용 모델에 적합하도록 데이터를 수정하는 방법과 UDM(User Define Model)을 개발하는 방법이 있다. UDM을 개발하는 것이 바람직하지만, 개발기간이 길고 UDM에 대한 검증의 필요성 때문에 범용 모델에 맞도록 데이터를 수정해야 할 필요가 있다. 이에 본 논문에 PSS/E 결과 데이터 또는 EMTDC 결과 데이터를 이용하여 CDC4 모델의 파라미터를 산정하는 방안을 제시하였다.

• The Microorganisms and Industry
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• v.17 no.1
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• pp.2-9
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• 1991

All of four genes are cloned and DNA sequence analysis have now revealed that these four genes encode a family of proteins with similar amino acid sequence. These proteins show no extensive similarities to any known proteins (Haarer et al., 1991). Among them, CDC3 gene is fused with E. coli lacZ and trpE genes and antibodies against the CDC3 gene product are produced. These antibodies are used to check the localization of this product to the vicinity of the 10 -nm filaments in the mother-bud neck.

• Proceedings of the Korean Information Science Society Conference
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• 2003.10a
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• pp.250-252
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• 2003

현재 무선단말기를 비롯한 많은 내장형 기기에서 자바 플랫폼을 채택하고 있다. 내장형 기기는 제공되는 메모리 용량의 제약으로 인하여 J2ME 플랫폼을 주로 사용하고 있다. J2ME 플랫폼은 CDC와 CLDC 컨피큐레이션으로 나뉘며. CDC는 JAVA 어플리케이션의 수행에 있어서 JVM보다 적은 동적 수행 메모리를 요구하는 CVM을 채택하고 있다. CDC는 CLDC에 비하여 메모리의 제약이 상대적으로 적은 셋탑박스 등과 같은 내장형 기기에서 주로 사용된다. 본 논문에서는 J2ME 플랫폼의 VM중에 하나인 CVM상에서 어플리케이션 수행에 따른 Generational GC의 특성에 대하여 알아본다. 특히 오브젝트의 life time, 가비지 컬렉션 주기 pause time 및 young generation의 크기에 따른 동작 특성을 중점적으로 고찰한다.

• Bulletin of the Korean Chemical Society
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• v.25 no.12
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• pp.1755-1756
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• 2004

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.6
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• pp.781-787
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• 2009

The sense and antisense digoxigenin-labeled RNA probes of four genes, Cdc25A, Cdc25B, Sox2 and Mnb, were produced by using SP6 and T7 RNA polymerases, respectively, and in vitro transcription. Expression patterns of the four genes were detected by in situ hybridization in HH (Hamburger and Hamilton) stage 10 chick embryos. In general, expression patterns of the four genes were similar. mRNA of the four genes was mostly restricted to the entire CNS (central nervous system). All were confined to an identical region, neural tube, neural groove and caudal neural plate, corresponding to the notochord or spinal cord, but there was some distinction in specific region or in concentration, for example in somites. The overlap in expression at the same developmental stage in the CNS suggests that the four genes may be functional similar or related in CNS development. Expression patterns of the four genes support specific roles of these regulators in the developing CNS.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.8
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• pp.3881-3885
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• 2016

Background: MicroRNAs (miRNAs) have fundamental roles in tumorigenesis. MiR-675 is upregulated in hepatocellular carcinoma(HCC) cells. However, the roles of miR-675 in hepatocellular carcinogenesis are still not fully elucidated. In this study, we focus on investigating the effect and mechanism of miR-675 in proliferation of HCC cells. Materials and Methods: The cell proliferation was measured by MTT assays after transfection with miR-675 inhibitor and miR-675 mimics in HCC cells. The expression level of miR-675 was detected by real-time quantitative reverse transcription polymerase chain reaction. Protein expression of Cdc25A was measured by western blotting analysis. Results: In MTT assays, overexpression of miR-675 promoted the proliferation of HCC cells(P<0.05. at 48 hours, P<0.01. at 72 hours) compared with the miR-675mimics control group. Downexpression of miR-675 inhibited the proliferation of HCC cells(P<0.05. at 48 hours, P<0.01. at 72 hours) compared with the miR-675inhibitor control group. In western blotting analysis, the expression level of Cdc25A was significantly increased (p<0.05) after treatment with miR-675 mimics. The expression level of Cdc25A was significantly decreased (p<0.05) after treatment with miR-675 inhibitor. Conclusions: Our results indicate that miR-675 promotes the proliferation in human hepatocellular carcinoma cells by associating with Cdc25A signaling pathway.