• Molecules and Cells
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• v.26 no.5
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• pp.462-467
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• 2008

TPA is known to cooperate with an activated Ras oncogene in the transformation of rodent fibroblasts, but the biochemical mechanisms responsible for this effect have not been established. In the present study we used c-fos promoter-luciferase constructs as reporters, in transient transfection assays, in NIH3T3 cells to assess the mechanism of this cooperation. We found a marked synergistic interaction between TPA and a transfected v-Ha-ras oncogene in the activation of c-fos promoter and SRE. SRE has binding sites for TCF and SRF. A dominant-negative Ras (ras-N17) inhibited the TPA-Ras synergy by blocking the PKC-MAPK-TCF pathway. Dominant-negative RhoA and Rac1 (but not Cdc42Hs) inhibited the TPA-Ras synergy by blocking the Ras-Rho-SRF signaling pathway. Constitutively active $PKC{\alpha}$ and $PKC{\varepsilon}$ showed synergy with v-Ras. These results suggest that the activation of two distinct pathways such as Ras-Raf-ERK-TCF pathway and Rho-SRF pathway are responsible for the induction of c-fos by TPA and Ras in mitogenic signaling pathways.

• Molecules and Cells
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• v.20 no.1
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• pp.97-104
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• 2005

To investigate apoptosis in HC11 mammary epithelial cells, we compared the gene expression profiles of actively growing and serum-starved apoptotic cells using a mouse apoptosis gene array and $^{33}P$-labeled cDNA prepared from the RNA of the two cultures. Analysis of the arrays showed that expression of several genes such as clusterin, secreted frizzled related protein mRNA (sFRP-1), CREB-binding protein (CBP), and others was higher in the apoptotic cells whereas expression of certain genes including survivin, cell division cycle 2 homolog A (CDC2), and cyclin A was lower. These expression patterns were confirmed by RT-PCR and/or Northern analyses. We compared the expression of some of these genes in the mouse mammary gland under various physiological conditions. The expression levels of genes (clusterin, CBP, and M6P-R) up-regulated in apoptotic conditions were higher at involution than during lactation. On the other hand, genes (Pin, CDC2) downregulated in apoptotic conditions were relatively highly expressed in virgin and pregnant mice. We conclude that certain genes such as clusterin, sFRP-1, GAS1 and CBP are induced in apoptotic mammary epithelial cells, and others are repressed. Moreover, the apoptosis array is an efficient technique for comparing gene expression profiles in different states of the same cell type.

• The Journal of Korean Medicine
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• v.34 no.3
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• pp.126-142
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• 2013

Objectives: Previous reports have shown that Bogijetong-Tang (BJT) is effective in peripheral neuropathy induced by taxol and crush injury. In this study, we researched the effects of BJT on diabetic neuropathy induced by STZ in the mouse. Methods: We performed both in vitro and in vivo experiments to verify the effects of BJT on diabetic neuropathy induced by STZ in mice. Changes in axonal recovery were observed with immunofluorescence staining using NF-200, Hoechst33258, $S100{\beta}$, caspase 3 and anti-cdc2. Proliferation and degeneration of Schwann cells were investigated by immunofluorescence staining and western blot analyses. Results: BJT showed considerable effects on neurite outgrowth and axonal regeneration in diabetic neuropathy. BJT contributed to the creation of NF-200, GAP-43, Cdc2, phospho-vimentin, ${\beta}1$, active ${\beta}1$, ${\beta}3$ integrin, phospho-Erk1/2 protein. Conclusions: Through this study, we found that BJT is effective for enhanced axonal regeneration via dynamic regulation of regeneration-associated proteins. Therefore, BJT had a pharmaceutical property enhancing recovery of peripheral nerves induced by diabetic neuropathy and could be a candidate for drug development after more research.

• ETRI Journal
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• v.27 no.3
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• pp.312-318
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• 2005

Mid-span spectral inversion (MSSI) has to utilize high optical pump power, for its operation principle is based on a nonlinear optical wavelength conversion. In this paper, a low pump-power operation of MSSI-based chromatic dispersion compensation (CDC) has been achieved successfully, for the first time to our knowledge, by introducing a noise pre-reduction scheme in cascaded wavelength conversions with periodically poled $LiNbO_3$ waveguides at a relatively low operation temperature. As preliminary studies, phase-matching properties and operation-temperature dependence of the wavelength converter (WC) were characterized. The WC pumped at 1549 nm exhibited a wide conversion bandwidth of 59 nm covering the entire C-band and a conversion efficiency of -23.6 dB at 11 dBm pump power. CDC experiments were implemented with 2.5 and 10 Gb/s transmission systems over 100 km single-mode fiber. Although it is well-known that the signal distortion due to chromatic dispersion is not critical at a 2.5 Gb/s transmission, the clear recovery of eye patterns was identified. At 10 Gb/s transmission experiments, eye patterns were retrieved distinctly from seriously distorted ones, and notable improvements in bit-error rates were acquired at a low pump power of 14 dBm.

• Transactions of the Korean Society of Automotive Engineers
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• v.19 no.2
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• pp.57-65
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• 2011

In this paper, a hydropneumatic modeling and dynamic analysis of a heavy truck semi-active cabin air suspension system is presented. Semi-active cabin air suspension system improves driver's ride comfort by controlling the damping characteristics in accordance with driving situation. So it can reduce vibration between truck frame and cabin. Semi-active cabin air suspension system is consist of air spring, leveling valve and CDC shock absorber, and full cabin system are mathematically modelled using AMESim software. Simulation results of components and full cabin system are compared with experimental data of components and test results of a cabin using 6 axis simulation table. It is found that the simulation results are in good agreements with test results, and the hydropneumatic model can be used well to predict dynamic characterics of heavy truck semi-active cabin air suspension system.

• Proceedings of the Korea Contents Association Conference
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• 2007.11a
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• pp.812-816
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• 2007

Having been speedy development of the IT industry, devices such as set-top box and smart phone are used in the broad filed. Because Java has merits that are platform independency, security and mobility, that is important software platform to offer stable services in the small device. This needs JVM(Java Virtual Machine) to execute Java application in the small device. CVM(Classic Virtual Machine) which is the kind of JVM is designed for embedded device to have limited resources. In this paper, CVM which is defined by CDC has designed and implemented on the Real-Time Operating System, UbiFOS$^{TM}$.

• Journal of Power Electronics
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• v.18 no.2
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• pp.395-406
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• 2018

Marine controlled-source electromagnetic transmitters (MCSETs) are important in marine electromagnetic exploration systems. They play a crucial role in the exploration of solid mineral resources, marine oil, and gas and in marine engineering evaluation. A DC-DC controlled-source circuit is typically used in traditional MCSETs, but using this circuit in MCSETs causes several problems, such as large voltage ringing of the high-frequency diode, heating of the insulated-gate bipolar transistor (IGBT) module, high temperature of the high-frequency transformer, loss of the duty cycle, and low transmission efficiency of the controlled-source circuit. This paper presents a clamping-diode circuit for MCSET (CDC-MCSET). Clamping diodes are added to the controlled-source circuit to reduce the loss of the duty ratio and the voltage peak of the high-frequency diode. The temperature of the high-frequency diode, IGBT module, and transformer is decreased, and the service life of these devices is prolonged. The power transmission efficiency of the controlled-source circuit is also improved. Saber simulation and a 20 KW MCSET are used to verify the correctness and effectiveness of the proposed CDC-MCSET.

• Journal of the Korean Chemical Society
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• v.26 no.4
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• pp.205-209
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• 1982

Anisotropic rotation of $CDCl_3$ in neat liquid is investigated by the analysis of ${\nu}_1$ band of Raman spectrum and the diffusion constant($D_{\perp}$) for the tumbling motion is obtained. The diffusion constant ($D_{II}$) for the spinning motion is obtained from the above $D_{\perp}$ value and the chlorine-35 nuclear quadrupole relaxation time. The diffusion constants thus obtained seem to agree very well with the ones obtained from $^2H$ and $^35C$l in NQR results within experimental errors. The data suggest fairly anisotropic character of reorientational motions in neat $CDCl_3$.

• BMB Reports
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• v.44 no.3
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• pp.147-157
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• 2011

The meiotic process from the primordial stage to zygote in female germ cells is mainly adjusted by post-transcriptional regulation of pre-existing maternal mRNA and post-translational modification of proteins. Several key proteins such as the cell cycle regulator, Cdk1/cyclin B, are post-translationally modified for precise control of meiotic progression. The second messenger (cAMP), kinases (PKA, Akt, MAPK, Aurora A, CaMK II, etc), phosphatases (Cdc25, Cdc14), and other proteins (G-protein coupled receptor, phosphodiesterase) are directly or indirectly involved in this process. Many proteins, such as CPEB, maskin, eIF4E, eIF4G, 4E-BP, and 4E-T, post-transcriptionally regulate mRNA via binding to the cap structure at the 5' end of mRNA or its 3' untranslated region (UTR) to generate a closed-loop structure. The 3' UTR of the transcript is also implicated in post-transcriptional regulation through an association with proteins such as CPEB, CPSF, GLD-2, PARN, and Dazl to modulate poly(A) tail length. RNA interfering is a new regulatory mechanism of the amount of mRNA in the mouse oocyte. This review summarizes information about post-transcriptional and post-translational regulation during mouse oocyte meiotic maturation.

• Membrane and Water Treatment
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• v.9 no.3
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• pp.195-203
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• 2018

N-acetylcysteine (NAC) has been widely used as an initial mucolytic agent and is generally used as an antioxidant to help alleviate various inflammatory symptoms. NAC reduces bacterial extracellular polymeric substances (EPS) production, bacterial adhesion to the surface and strength of mature biofilm. The efficacy has been shown to inhibit proliferation of gram-positive and gram-negative bacteria. In membrane bioreactor (MBR) processes, which contain a variety of gram negative bacteria, biofilm formation has become a serious problem in stable operation. In this study, use of NAC as an inhibitor of biofilm contamination was investigated using the center for disease control (CDC) reactors with MBR sludge. Biomass reduction was confirmed with CLSM images of membrane surfaces by addition of NAC, which was more efficient as the concentration of NAC was increased to 1.5 mg/mL. NAC addition also showed decreases in EPS concentrations of the preformed biofilm, indicating that NAC was able to degrade EPS in the mature biofilm. NAC addition was also effective to inhibit biofilm formation by MBR sludge, which consisted of various microorganisms in consortia.