• Laboraroty Animal Research
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• 제34권4호
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• pp.302-310
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• 2018

CD47 (integrin-associated protein), a multi-spanning transmembrane protein expressed in all cells including red blood cells (RBCs) and leukocytes, interacts with signal regulatory protein ${\alpha}$ ($SIRP{\alpha}$) on macrophages and thereby inhibits phagocytosis of RBCs. Recently, we generated a novel C57BL/6J CD47 knockout ($CD47^{-/-}$ hereafter) mouse line by employing a CRISPR/Cas9 system at Center for Mouse Models of Human Disease, and here report their hematological phenotypes. On monitoring their birth and development, $CD47^{-/-}$ mice were born viable with a natural male-to-female sex ratio and normally developed from birth through puberty to adulthood without noticeable changes in growth, food/water intake compared to their age and sex-matched wild-type littermates up to 26 weeks. Hematological analysis revealed a mild but significant reduction of RBC counts and hemoglobin in 16 week-old male $CD47^{-/-}$ mice which were aggravated at the age of 26 weeks with increased reticulocyte counts and mean corpuscular volume (MCV), suggesting hemolytic anemia. Interestingly, anemia in female $CD47^{-/-}$ mice became evident at 26 weeks, but splenomegaly was identified in both genders of $CD47^{-/-}$ mice from the age of 16 weeks, consistent with development of hemolytic anemia. Additionally, helper and cytotoxic T cell populations were considerably reduced in the spleen, but not in thymus, of $CD47^{-/-}$ mice, suggesting a crucial role of CD47 in proliferation of T cells. Collectively, these findings indicate that our $CD47^{-/-}$ mice have progressive hemolytic anemia and splenic depletion of mature T cell populations and therefore may be useful as an in vivo model to study the function of CD47.

• BMB Reports
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• 제47권5호
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• pp.241-248
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• 2014

CD1 molecules belong to non-polymorphic MHC class I-like proteins and present lipid antigens to T cells. Five different CD1 genes (CD1a-e) have been identified and classified into two groups. Group 1 include CD1a-c and present pathogenic lipid antigens to ${\alpha}{\beta}$ T cells reminiscence of peptide antigen presentation by MHC-I molecules. CD1d is the only member of Group 2 and presents foreign and self lipid antigens to a specialized subset of ${\alpha}{\beta}$ T cells, NKT cells. NKT cells are involved in diverse immune responses through prompt and massive production of cytokines. CD1d-dependent NKT cells are categorized upon the usage of their T cell receptors. A major subtype of NKT cells (type I) is invariant NKT cells which utilize invariant $V{\alpha}14-J{\alpha}18$ TCR alpha chain in mouse. The remaining NKT cells (type II) utilize diverse TCR alpha chains. Engineered CD1d molecules with modified intracellular trafficking produce either type I or type II NKT cell-defects suggesting the lipid antigens for each subtypes of NKT cells are processed/generated in different intracellular compartments. Since the usage of TCR by a T cell is the result of antigen-driven selection, the intracellular metabolic pathways of lipid antigen are a key in forming the functional NKT cell repertoire.

• 분석과학
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• 제13권3호
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• pp.368-377
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• 2000

실리카고정괭생이모자반을 합성하여 중금속 흡착제로 이용하였다. 이 흡착제에 대한 중금속 Cd(II), Pb(II)이온의 최대흡착조건을 알아보고 흡착특성을 연구하였다. 그 결과 산성보다는 알카리성에서 흡착량이 더 크고 Si-고정괭생이모자반에 대한 중금속 Cd(II), Pb(II)이온의 흡착량이 더 큼을 알 수 있었다. 또한 Cd(II) 이온보다 Pb(II) 이온의 흡착량이 더 높음을 알 수 있었다. Cd(II) 및 Pb(II) 이온의 회수율은 괭생이모자반에서 58.0-62.6%, 61.2-64.4%로 나타났고, Si-고정괭생이모자반에서 56.8-92.7%, 37.8-47.9%로 나타났다. Cd(II)이온은 Si-고정괭생이모자반에서의 회수율이 더 높게 나타났으나, Pb(II) 이온은 Si-고정괭생이모자반에서의 회수율이 더 낮게 나타났음을 알 수 있었다.

• Journal of Plant Biotechnology
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• 제47권4호
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• pp.324-329
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• 2020

Reed (Phragmites spp.) is a rhizomatous plant of the Poaceae family and is known as high tolerant plant to heavy metal contaminants. This plant is widely recognized as a Cd root-accumulator, but improved heavy metal tolerance and uptake capacity are still required for phytoremediation efficiency. To enhance capacity of hyperaccumulator plants, ethyl methane sulfonate (EMS) as chemical mutagen has been introduced and applied to remediation approaches. This study aimed to select EMS-mutagenized reeds representing high Cd resistance and large biomass and to investigate their ability of Cd accumulation. After 6 months cultivation of M₂ mutant reeds under Cd stress conditions (up to 1,500 µM), we discovered seven mutant individuals that showed good performances like survivorship, vitality, and high accumulation of Cd, particularly in their roots. Compared to wild type (WT) reeds as control, on average, dry weight of mutant type (MT) reeds was larger by 2 and 1.5 times in roots and shoots, respectively. In addition, these mutant plants accumulated 6 times more Cd, mostly in the roots. In particular, MT8 reeds showed the greatest ability to accumulate Cd. These results suggest that EMS mutagenesis could generate hyperaccumulator plants with enhanced Cd tolerance and biomass, thereby contributing to improvement of phytoremediation efficiency in Cd-contaminated soil or wastewater. Further studies should focus on identifying Cd tolerance mechanisms of such EMS-mutagenized plants, developing techniques for its biomass production, and investigating the practical potential of the EMS mutants for phytoremediation.

• The Korean Journal of Ecology
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• 제13권3호
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• pp.173-180
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• 1990

The contents of Pb and CD measured with the barks of Pinus densiflora in Seoul area, Yangjaedong area near the Kyungbu-highway, Mt. Keryong and Mt. Seolak area, from Sep. to Ock. in 1988. Pb contents in Seoul area were 17.8 ppm at Changkyungkung, 17.1 ppm at Chongamdong, 13.2 ppm at Mt. Kwanak, 7.6 ppm at Mt. Dobong and 3.7 ppm at Mt. Surak. Except Mt. Kwanak, the difference between areas of central and mountains in Seoul were high significant. Mt Seolak area also showed variations in Pb contents from 16.8 ppm to 32.1 ppm according to studied sites. Cd contents in Seoul area were 0.47 ppm at Changkyungkung, 0.65 ppm at Chongamdong, 0.61 ppm at Mt. Kwanak, 0.52 ppm at Mt. Dobong, 0.47 ppm at Mt. Surak. Therefore, Cd contents in Seoul area exhibited more variations between sampling plots than between the areas of central and mountains in Seoul. Mt Seolak area also showed variations from 0.20 ppm to 0.34 ppm in Cd contents, and it was the lowest in all the studied area.

• 한국세라믹학회지
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• 제47권2호
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• pp.189-194
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• 2010

Compared with organic fluorophores, semiconductor quantum dots (QDs) have the better properties such as photostability, narrow emission spectra coupled to tunable photoluminescent emissions and exceptional resistance to both photo bleaching and chemical degradation. In this work, CdSe/ZnS QDs nanobeads were prepared by the incorporation of CdSe/ZnS QDs with mesoporous silica to use as the optical probe for detecting toxic and bio- materials with high sensitivity, CdSe/ZnS core/shell QDs were synthesized from the precursors such as CdO and zinc stearate with the lower toxicity than pyrotic precursors. The QD-nanobeads were characterized by transmission electron microscopy, FL microscopy, UV-Vis and PL spectroscopy, respectively.

• Journal of Ginseng Research
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• 제47권1호
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• pp.155-158
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• 2023

In the present study, we investigated whether treatment with KRG improve the parameters of immune activity such as the cytotoxicity, populations of CD4⁺ CD8⁺T cell, CD3^-CD172^-CD8⁺ NK cell and CD172⁺ monocyte as well as natural cytotoxicity receptors such as Nkp46, Nkp44, Nkp30. In results, KRG significantly increased these immune activities. These results indicate that KRG has distinct immuneenhancing effects by increasing the roles of T cells and NK cell in porcine.

• 원예과학기술지
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• 제29권5호
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• pp.413-419
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• 2011

국내 자생 수생식물인 노랑꽃창포와 창포를 수질 내 카드뮴(Cd) 제거용 식물재료로 이용하고자 Cd 축적과 내성을 조사하였다. Cd 농도에 따른 치사율을 근거로 Cd에 대한 반수치사량($LD_{50}$)을 계산한 결과, 노랑꽃창포는 $78.5{\mu}M$, 창포는 $47.6{\mu}M$로 나타났다. Cd 스트레스에 대해 노랑꽃창포에서는 POD와 SOD, 창포에서는 CAT와 SOD, 폴리페놀이 활성화되었다. 노랑꽃창포에서는 폴리페놀이 측정되지 않았다. 노랑꽃창포와 창포 모두 Cd 처리 농도가 증가하고 기간이 길어질수록 식물체 내 Cd 축적량이 증가하였고, 두 종 모두 지상부보다는 지하부 축적량이 현저하게 많았다. 지상부에 축적된 Cd 함량은 노랑꽃창포 $548.1mg{\cdot}kg^{-1}$(지하부 축적 대비 82.1%) 창포 $121.4mg{\cdot}kg^{-1}$(지하부 축적 대비 13.7%)로 두 종 모두 Cd 고축적식물로 확인되었으며, 특히 노랑꽃 창포가 Cd 축적능력과 내성이 우수하였다.

• 한국자기공명학회논문지
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• 제4권1호
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• pp.41-49
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• 2000

To design more efficient peptide antagonist against the HBV, preSl(21-47) which carries the HBV receptor binding site for hepatocytes was synthesized and the solution structure of preSl(21-47) was investigated using CD spectroscopy and NMR spectroscopy in membrane-mimicking environments. According to CD spectra, preSl(21-47) has a random structure in aqueous solution, while conformational change was induced by addition of TFE and SDS micelle. Tertiary structures as determined by NMR spectroscopy shows that preSl(21-47) has a very flexible structure even in SDS micelle.

• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2002년도 춘계 국제 학술대회
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• pp.47-48
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• 2002

The purpose of this study was to investigate the metallothionein of acute cadmium poisoning mice as a Cadmium index. Forty male ICR mice were injected with cadmium chloride solution from 1/8LD$\sub$50/ to 1/2 LD$\sub$50/ dose. At 24 hours after exposed Cd, I examined Cd and metallothionein (MT) in tissues (liver and kidney) and fluids (whole blood and urine) and also measured low molecular proteins, N-acety1-${\beta}$-D-glucosaminidase (NAG) and ${\beta}$$\sub$2/- microglobuline (${\beta}$$\sub$2 /-MG) in urine. The concentration of Cd and MT of liver, kidney whole blood and urine were increased with dose dependent manner. Urinary Cd and urinary MT had very good significance (p＜0.01) and urinary MT had good significance with kidney Cd and NAG but not ${\beta}$$\sub$2/-MG. Conclusionally MT in urine was very correlated with kidney Cd and urine Cd. So MT maybe useful as a Cd poisoning index.