• Journal of Korean Library and Information Science Society
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• v.30 no.2
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• pp.123-153
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• 1999

This article aims at finding the ways, on the basis of the studies about the behaviors to search the existing CD-ROM databases, so that the searchers who retrieve the on-line MEDLINE used in the medical libraries can use the data more efficiently than now. We gave the questionnaires to the librarians in 60 medical libraries and searched the literatures and realities on the behaviors of the data uses to examine the search behaviors of the MEDLINE in the medical libraries. The result is as follows: 1) The medical data system rate for single users was $53\%$ and the ons for multi users $43\%$. As for the time which users retrieve for a week, under two hours was $75\%$, between 3 and 8 hours $18.3\%$, and eve. 9 hours $6.7\%$. 2) The increasing factors of the search result are (1) an enough discussion and interview between librarians and users, and (2) the use of the correct indexing terms, Thesaurus, and Keyword. In principle users must search directly. However, the librarians searched instead in case that the retrieval result was under two hours a week$(75\%)$. 3) As for the search fee, $91\%$ was free and $9\%$ was charged. Also search effectiveness was enhanced by the means of Inter-Library Loan Service & Information Network. 4) The medical librarians answered the questionnaire that they need the application education of professional knowledge, medical terms(thesaurus) and electronic medium, and also they need the computer education, interview technique and reeducation to give a satisfactory service. 5) As for the satisfactory degree of MEDLINE application, they answered $44.6\%$ for economy, $38.2\%$ for the conveniency of the time required, and $58.9\%$ for the users' search satisfaction answered respectively. 6) The application of MEDLINE system enhanced the medical libraries' image and had an effect on the users' satisfaction of using the data and search, the data activities and the research achievement. 7) In the past MeSH was used but as the time passes CD-ROM MEDLINE search behavior was preferred to On-line one.

• Journal of Environmental Health Sciences
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• v.43 no.2
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• pp.103-110
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• 2017

Objectives: This study was performed to provide basic data for the re-establishment of standards (criteria) and analytical methods for hazardous metals in nail enamel. Methods: Ten metals (lead; Pb, arsenic; As, cadminum; Cd, antimony: Sb, cobalt; Co, nikel; Ni, copper; Cu, chromium; Cr, aluminum; Al, and mercury; Hg) were measured in 67 commercial nail enamels containing glitter and/or pearls. The content of hazardous metals (excepting Hg) was determined by using an inductively coupled plasma-optical emission spectrophotometer (ICP-OES) after microwave digestion. Mercury content was measured by a mercury analyzer without any preparation. Results: The detected ranges of the intact samples were as follows: $ND-1.756{\mu}g/g$ for Pb, $ND-1.24{\mu}g/g$ for As, ND for Cd, $ND-20.41{\mu}g/g$ for Sb, $ND-12.36{\mu}g/g$ for Co, $ND-7.908{\mu}g/g$ for Ni, $0.088-79.27{\mu}g/g$ for Cu, $0.281-18.54{\mu}g/g$ for Cr, $13.78-3563{\mu}g/g$ for Al, and $ND-0.044{\mu}g/g$ for Hg. After centrifugation, the detected ranges of supernatant were as follows: $ND-0.435{\mu}g/g$ for Pb, $ND-0.504{\mu}g/g$ for As, ND for Cd, $ND-0.035{\mu}g/g$ for Sb, $ND-13.17{\mu}g/g$ for Co, $ND-0.232{\mu}g/g$ for Ni, $0.117-90.07{\mu}g/g$ for Cu, $0.174-2.787{\mu}g/g$ for Cr, and $9.459-1565{\mu}g/g$ for Al. The results of this analysis showed that the levels of heavy metals such as Pb, As, and Sb were much higher in the intact samples than those of supernatant. Conclusion: In the present study, we found that the levels of hazardous metals were significantly different depending on the status of the presence of glitter. Based on the results, we recommend that the product consumer refrain from prolonged application of nail enamel, avoid biting or chewing the nails, and wear gloves during cooking and washing dishes.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.7
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• pp.3431-3436
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• 2016

Background: Carcinogenesis is a multifaceted intricate cellular mechanism of transformation of the normal functions of a cell into neoplastic alterations. Metastasis may result in failure of conventional treatment and death Hence, research on metastatic suppressors in cancer is a high priority. The metastatic suppressor gene CD82, also known as KAI1, is a member of the transmembrane 4 superfamily which was first identified in carcinoma of prostate. Little work has been done on this gene in breast cancer. Herein, we aimed to determine the gene and protein level expression of CD82/KAI1 in breast cancer and its role as a prognosticator. Materials and Methods: In this study, 83 histologically proven cases of breast cancer and a similar number of controls were included. Patient age ranged from 18-70 years. Quantitative Real Time Polymerase Chain Reaction (q-RT PCR) and immunohistochemistry (IHC) were used to investigate KAI1 expression at gene and protein levels, respectively. Statistical analysis was done to correlate expression of KAI1 and clinicopathological parameters. Results: It was revealed that: (i) KAI1 was remarkably diminished in metastatic vs non metastatic breast cancer both at the gene and the protein levels (P < .05); (ii) KAI1 expression levels were strongly correlated with TNM staging, histological grade and advanced stage (p<0.001) and no association was found with any other studied parameter; (iii) Lastly, a significant correlation was observed between expression of KAI1 and overall median survival of BC patients (P = 0.04). Conclusions: Our results suggest that lack of expression of the KAI1 might indicate a more aggressive form of breast cancer. Loss of KAI1 may be considered a significant prognostic marker in predicting metastatic manifestation. When evaluated along with the clinical and pathological factors, KAI1 expression may be beneficial to tailor aggressive therapeutic strategies for such patients.

• Korean Journal of Veterinary Research
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• v.34 no.2
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• pp.287-299
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• 1994

Plasma protein which has been known as one of nonspecific immunostimulators was added to feedstuff to examine its effect on the enhancement of cellular immune response in porcine immune system. A total of 40 piglets, 20 male and 20 female each, were fed for 30 days with or without plasma protein. The peripheral blood were collected and analyzed for the investigation of leukocyte subpopulations and their activities by using a panel of monoclonal antibodies specific to porcine leukocyte differentiation antigens and flow cytometry. The results obtained as follows. 1. Subpopulations expressing major histocompatibility complex(MHC) class I antigen were $96.2{\pm}3.1%$ and $86.6{\pm}3.8%$ in piglets fed with plasma protein and in piglets fed without plasma protein, respectively. 2. Proportion of leukocyte subpopulation expressing MHC class II antigens were significantly higher in the piglets fed with plasma protein than ones without plasma protein. The proportion was $27.6{\pm}3.6%$ and $16.6{\pm}2.2%$ in MHC class II DQ antigen, and $28.1{\pm}2.0%$ and $20.0{\pm}0.3%$ in MHC class II DR antigen, respectively. 3. A significant increase in the proportion of cells expressing poCD2 was not found in piglets fed plasma protein. 4. Proportion of subpopulation expressed porcine(Po) CD4 antigens which specific to helper T lymphocytes were not increased (18.3-19.1% vs. 25.6-28.8%), rather slightly decreased, in plasma protein-treated group. 5. The most important increase of proportion in plasma protein-treated group was the leukocyte subpopulation specific to $poCD8^+$ T cytotoxic/suppressor lymphocytes. The expression level was significantly higher up to 45.9-47.1% in plasma protein-treated group in comparing with 29.7-33.0% in non-plasma protein-treated group. 6. Lymphoblastogenetic responses using different concentrations of Con A mitogen and plasma protein has found that the responses of lymphocyte from piglets fed plasma protein was significantly activated (p<0.01). The activities measured by 3[H]-thymidine incorporation showed 3-6 times stronger in plasma protein-treated group than those in non-plasma protein-treated group. The study has concluded that plasma protein, which has known as a nonspecific immunostimulator, may have an immunoenhancing activities in porcine lymphoid system by increase the activated cell proportions and their blastogenetic properties which is critical to host immune responses.

• Korean Journal of Food Science and Technology
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• v.35 no.5
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• pp.812-817
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• 2003

This study was conducted to estimate the contents of heavy metals in tea products (n=200) and to provide a scientific basis for standardization of heavy metals in the Korea Food Code. The contents of mercury (Hg), lead (Pb), cadmium (Cd), arsenic (As), copper (Cu), manganese (Mn), zinc (Zn), and aluminium (Al) were determined using a mercury analyzer, an AAS (atomic absorption spectrophotometer), and ICP (inductively coupled plasma spectrometer). The values of heavy metals in tea products were [minimum-maximum (mean), mg/kg] Hg: $N.D.{\sim}0.02$ (0.003), Pb: $N.D.{\sim}0.35$ (0.03), Cd: $N.D.{\sim}0.07$ (0.007), As: $N.D.{\sim}0.19$ (0.006), Cu: $N.D.{\sim}24.41$ (2.73), Mn: $N.D.{\sim}767.88$ (66.91), Zn: $N.D.{\sim}18.60$ (4.54), Al: $N.D.{\sim}1304.30$ (85.53). These results showed that metal contents in tea products in our markets were similar to those reported in other countries. The average weekly intakes of lead, cadmium, and mercury from tea products were $0.1{\sim}0.2%$ of PTWI (Provisional Tolerable Weekly Intake) that the JECFA (Joint FAO/WHO Expert Committee on Food Additives) has set to evaluate their safeties.

• Korean Journal of Veterinary Service
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• v.35 no.3
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• pp.223-230
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• 2012

This study was carried out to investigate the heavy metal and microbiological hazards on by-products (liver, omasum, small intestines) of cattle. From April to October in 2011, one hundred and twenty samples were equally collected from slaughterhouse and meat by-product markets in Incheon city. The total bacteria counts and E. coli count were applied to assess the microbiological quality. Food borne bacteria including Salmonella spp., Listeria monocytogenes, Staphylococcus aureus, Clostridium perfringens and E. coli O157:H7 were also determined. The results were obtained as follows: The undesirable grade (more than $10^5\;CFU/cm^2$) was detected in the by-product from 18.3% (slaughterhouse) and 23.3% (by-product markets). The frequency of generic E. coli (more than $10^2\;CFU/cm^2$) from the slaughterhouse was 20.0%, whereas that of the meat by-product markets was 26.7%. Of the samples from slaughterhouse, 3 (5.0%), 5 (8.3%), and 12 (20.0%) samples were contaminated with Salmonella spp, S. aureus, and C. perfringens, respectively. S. aureus and C. perfringens were also detected in 6 (10.0%) and 25 (41.7%) samples in the meat by-product markets, respectively. Nine of 11 S. aureus isolates harbored toxin gene. However, the cpe gene of C. perfringens was not detected among the 37 isolates. The detection rate was higher in August than in February, April and June. The levels of Cd and Pb in all the samples tended to be low (<0.2 mg/kg). This preliminary data could be used for legislation on the regulation and control of microorganism and heavy metal in by-products of cattle.

• Korean Journal of Soil Science and Fertilizer
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• v.50 no.5
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• pp.472-481
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• 2017

Agricultural field near at the abandoned metal mine and industrial area has a high possibility to be polluted by heavy metals. However, concern about chemical properties including heavy metal concentration has been increased and biological properties such as soil respiration has been minimal in heavy metal polluted field. Therefore, main objective of this research was to evaluate soil respiration as an indicator of heavy metal pollution in agricultural field. Total of 60 sampling sites including each 30 sites of abandoned metal mine and industrial area were selected and heavy metal concentration, soil respiration, and chemical properties were measured. Results showed that heavy metal concentration in metal mine area was ranged Cu: $6.21~85.23mg\;kg^{-1}$, Pb: $23.84{\sim}1,044.72mg\;kg^{-1}$, As: $1.88{\sim}691.44mg\;kg^{-1}$, Zn: $18.72{\sim}527.55mg\;kg^{-1}$, Cd: $0.58{\sim}4.27mg\;kg^{-1}$, and Cu: $0.29{\sim}30.62mg\;kg^{-1}$, Pb: $4.41{\sim}19.77mg\;kg^{-1}$, As: $2.23{\sim}11.76mg\;kg^{-1}$, Zn $39.98{\sim}109.59mg\;kg^{-1}$, Cd $0.29{\sim}0.57mg\;kg^{-1}$ for industrial area respectively. While no sampling site was exceed the threshold value of each heavy metals in industrial field, metal mine area was highly polluted with Pb, As, Zn, and Cd. Soil respiration in the metal mine and industrial area was ranged $12.05{\sim}299.80mg\;O_2\;kg^{-1}$ and $27.68{\sim}330.94mg\;O_2\;kg^{-1}$, respectively. Correlation analysis between heavy metal concentration in soil and soil respiration showed that negative correlation was observed in metal mine area while no correlation was observed in industrial area. This result might indicate that as heavy metal concentration was increased, microbial activity in soil was decreased resulting decrease of soil respiration rate. Overall, soil respiration can be used as indicator of heavy metal pollution in soil and more biological properties need to be evaluated to better understand heavy metal pollution in soil.

• The Korean Journal of Mycology
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• v.48 no.2
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• pp.151-160
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• 2020

The goal of this study was to investigate the diversity present among wild yeasts obtained from soils of spice fields and from mountain soils, and to further, characterize previously unrecorded novel wild yeast strains. In total, 36 strains from 17 different species of wild yeasts were isolated from 35 soil samples obtained from garlic fields of Geumsan, Chungcheongnam-do, Korea. Among these, six yeast strains of Trichosporon moniliiforme, and four strains each of Papiliotrema flavescens and Candida melibiosica species were isolated. Additionally, 22 strains of 18 different species of wild yeasts were isolated from 32 soil samples collected from the ballonflower and ginger fields of Geumsan, Korea. Finally, 46 strains of wild yeasts were isolated from 35 soil samples obtained from Mt. Daedun in Geumsan, Korea. Among the total of 106 isolated wild yeast strains, 10 strains, including Debaryomyces vindobonensis GHY31-3 represented novel yeast strains which were previously unrecorded. All the 10 previously unrecorded yeasts were oval or global in shape, and five strains, including Filobasidium stepposum SFG1-4 formed ascospores. Three strains, including Pseudozyma alboarmeniaca CD 23-5 grew well in vitamin-free medium. Cell-free extract obtained from Filobasidium magnum SFG1-3 indicated 28.6% of xanthine oxidase inhibitory activity.

• Proceedings of the KSEEG Conference
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• 2003.04a
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• pp.241-244
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• 2003

유류오염 토양내 유류의 분해와 무기오염물질의 함량과의 관계성을 알아보기위해 부산 문헌지구의 유류 오염 토양 profile중 유류가 비교적 많은 두 층과 유기물이 다량 함유된 제주도 토양을 경유로 오염시킨 토양을 선택하여 유류가 분해 정도와 Pb, Zn, Cd, Ni의 함량을 측정 비교하였다. 토양 채취후 즉시 유류를 추출한 것, 15일간 별도의 미생물 영양원을 가하지 않고 습도와 통풍만을 행하여 토양내 존재하는 미생물들에 의하여만 유류가 분해되도록 한 것, $105^{\circ}C$에서 24시간 열처리를 행하여 미생물에 의한 분해보다는 물리적인 방법으로 비교적 강하게 처리한 것 세가지 경우에서 토양에서 추출한 유류를 분석한 결과 normal alkane이 서서히 상당량 분해되는 양상을 보여주었다. 토양내 Pb, Zn, Cd, Ni를 분석한 결과 부산 시료의 경우 두 층(B2, B3)모두 Cd은 검출한계 이하로, Ni의 경우 극소량의 함량을 보여주었다. Zn의 경우 B2는 28～29ug/g, B3는 12～14ug/g으로 세가지 경우 별 차이 없는 양상을 보여 주었다. 하지만, Pb의 경우 B2에서는 그 함량이 유류의 normal alkane의 분해와 함께 서서히 증가하는 경향을 확연히 보여주었으며, B3의 경우도 B2와 유사한 변화 양상을 보여주었다. 제주도 토양의 경우 Pb는 검출되지 않았고, Zn은 소량 검출되었다. Ba, Ni 함량은 normal alkane 분해에 따라 다소 증가하였다. 따라서, 본 예비실험을 통하여 유류오염 토양에서 유류의 분해와 일부 무기이온사이에는 관련성을 갖고 있으며, 이는 유류 분해정도를 파악하는 지시자로써 특정 무기 오염물질을 이용할 수 있을 가능성이 있으므로 좀더 이들 관계성에 대한 연구가 진행될 필요성이 있다고 판단된다.고 과학적으로 분석할 수 있는 방법이 될 수 있을 것으로 기대된다. 의미를 되새기는 것으로 짧은 연구를 시작하겠다. 등은 활성 값이 70% 이상으로 퇴적물 독성이 상대적으로 낮았다. 이중나선 DNA 함량은 28.4 % - 49%로 대조군에 비해서 감소가 크다. 대부분의 정점이 대조군의 30% 내외로 정점 간의 차이는 크지는 않다. 그러나 다른 측정자료와 같이 정점 22에서 18%로 최소치를 나타내고, 정점 2, 12에서 20% 내외의 값을 보인다. 종합적으로 볼 때 오염물질의 유입이 크고, 광양제철 인근 정점 들이 모두 다른 정점에 비해서 낮아서, 퇴적물 독성이 높은 정점으로 조사되었다.hiwo의 광합성 능력은 낮은 농도들에서는 대조구와 유사하였으나, 5 $\mu\textrm{g}$/l의 높은 농도에서는 초기에 매우 낮은 광합성 능력을 보이다가 시간이 경과하면서 대조군보다 더 높은 경향을 나타냈다. 이러한 결과는 식물플랑크톤이 benso[a]pyrene의 낮은 농도에서 노출될 때는 이 물질을 탄소원으로 사용할 가능성이 있음을 시사한다. 본 연구의 결과들은 연안해역에 benso[a]pyrene과 같은 지속성 유기오염물질이 유입되었을 때 내정여부에 따라 식물플랑크톤 군집내 종 천이와 일차생산력에 크게 영향을 미칠 수 있음을 시사한다.TEX>5.2개)였으며, 등급별 회수율은 각각 GI(8.5%), GII(13.4%), GIII(43.9%), GIV(34.2%)로 나타났다.ments of that period left both in Japan and Korea. "Hyojedo" in Korea is supposed to have been influenced by the letter design. Asite- is also considered to have been "Japan

• Journal of Korean Medicine Rehabilitation
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• v.29 no.4
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• pp.109-115
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• 2019

Cervical dystonia (CD) is a disorder characterized by sustained or intermittent, involuntary muscle contractions which cause twisting, repetitive movements and abnormal postures. In this case report, a CD patients was treated with transfer energy capacitive and resistive (TECAR) therapy and conventional Korean medicine, which consists of acupuncture, Chuna manual medicine, pharmacopuncture and herbal medicine. For outcome measures, this study evaluated Cobb's angle, EuroQol five dimension scale (EQ-5D), numerical rating scale (NRS), neck disabillity index (NDI) and cervical range of motion. As a result, improvements were found in Cobb's angle ($18.65^{\circ}$ to $15.90^{\circ}$), EQ-5D score (0.808 to 0.862), NRS (5 to 3), NDI (16 to 8) and angle of cervical lateral bending ($15^{\circ}$ to $25^{\circ}$). In conclusion, this study shows that combined therapy of conventional Korean medicine treatment and TECAR therapy can be effective for CD patients.