• Title/Summary/Keyword: CD138

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Effect of Dietary Cadmium Level on Clinical Toxicity, Sperm Capacity and Histopathological Changes in Rats (카드뮴 투여수준이 흰쥐의 임상적인 중독증과 정자능력 및 장기조직에 미치는 영향)

  • 한찬규;노정해;이남형;채찬희;김광식;이복희
    • Korean Journal of Animal Reproduction
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    • v.23 no.1
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    • pp.1-12
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    • 1999
  • The present study was conducted to find the effects of different cadmium(Cd) levels in diets on clinical toxicity, sperm capacity and histopathological changes in rats. Thirty male rats of Sprague-Dawley weighing 125.3$\pm$15.2g were randomly blocked into five groups according to body weights. Five levels of Cd in AIN-76 purified diet(0, 25, 50, 100 and 250 ppm) had been fed for 8 weeks. Cadmium was supplemented with a form of CdCl$_2$. 1. After 8 weeks of Cd intake had resulted in apparent cadmium intoxication; reduced growth rate, enlarged kidney and testis, decreased hematocrit value and hemoglobin content in response to supplemented Cd levels in the diets. 2. Cadmium accumulation in liver and kidney showed a tendency to increase in cadmium-exposed groups. The levels of metallothionein were also significantly elevated in the tissues of liver in response to the levels of Cd supplemented(P<0.05). 3. Although sperm motility was not significantly different among treatments, rats fed Cd tended to have reduced sperm motility but sperm concentration of Cd supplemented groups were significantly lower than that of control(p<0.05). 4. Based on the findings from gross lesion, rats fed 250ppm of Cd were externally emaciated, had exposed penis and observed atrophies of kidney and testis. Histopathological observation seemed that the liver of groups feeding Cd supplemented diets showed cellular degeneration and accumulation of eosinophilic materials in the capillaries. In kidney, rats fed Cd diets had shown tubular epithelium degeneration and lesions of basophilic materials, while testes were weakened in numbers of spermatid and sporadically enlarged of giant cells.

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Analysis of the Transport System of Cadmium and the Change of Proline Content in Spring Radish Young Plant (봄무우 유식물에서 카드뮴 운반계와 Proline 농도 변화의 분석)

  • Park, Sun Young;Park, Myon-Yong;Cho, Bong-Heuy
    • Analytical Science and Technology
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    • v.9 no.2
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    • pp.134-138
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    • 1996
  • Cadmium was transported through the special carrier system into the cells of cotyledons, hypocotyls, and roots spring radish young plants. The transport of cadmium was inhibited by metabolic inhibitor, like DNP. The $K_m$ values for cadmium were 0.7 ppm for cotyledons, 1.72 ppm for hypocotyls, and 0.3 ppm for roots, and $V_{max}$ for cadmium was $40ppm{\cdot}h^{-1}{\cdot}g{\cdot}fresh\;weight^{-1}$ for cotyledons, $313ppm{\cdot}h^{-1}{\cdot}g{\cdot}fresh\;weight^{-1}$ for hypocotyls, and $606ppm{\cdot}h^{-1}{\cdot}g{\cdot}fresh\;weight^{-1}$ for roots. Cadmium cannot prove to be inducer for proline accumulation. Therefore, proline accumulation cannot be used as a marker to test the level of heavy metal pollution.

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Effects of heavy metals on the degradation of fenitrothion, IBP, and butachlor in flooded soil (담수토양중(湛水土壤中)에 있어서 fenitrothion, IBP, butachlor의 분해(分解)에 미치는 중금속(重金屬)의 영향(影響))

  • Moon, Young-Hee
    • Applied Biological Chemistry
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    • v.33 no.2
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    • pp.138-142
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    • 1990
  • The effects of heavy metals Cd, Cu, Cr, Ni, and Zn on the degradation of the insecticide fenitrothion (O, O-dimethyl O-4-nitro-m-tolyl phosphorothioate), the fungicide IBP (5-benzyl O, O-diisopropyl phosphorothioate), and the herbicide butachlor (N-butoxymetyl-2-chloro-2', 6'-diethylacetanilide) in flooded soils were examined in the laboratory. The degradation of the 3 pesticides in soil was greatly inhibited by the amendment of the 5 heavy metals. The inhibition rate was high in the order of butachlor>IBP>fenitrothion. Populations of fenitrothion-and butachlor-degrading microbes, which were counted by the MPN method, were lower in heavy metals added soil than in the control soil. The effect of heavy metals on the degradation of the 3 pesticides in soil varied with the kind and concentration of heavy metals and the kind of pesticides.

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Study on the chemical forms of heavy metals in the surface sediments of Ulsan Bay

  • Kim, Young-Bok;Jo, Sun-Young;Jeong, Jong-Hak;Lee, Sung-In;Jeong, Gi-Ho
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.2 no.1
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    • pp.69-78
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    • 1998
  • Contents of heavy metals (Cr, Cu, Zn, Cd, and Pb) in the sediments of Ulsan Bay were investigated by the sequential extraction methods that classifies heavy metals into five types of chemical forms: exchangeable, bound to carbonates, bound to Fe-Mn oxides, bound to organic matter and residual. The analytes were determined by using an ICP-MS. Total Cr concentrations in the sediments were in the range of 41.6-96.4, Cu 60.7-680, Zn 189-1954, Cd 33.1-83.4, and Pb 138-567 mg/kg. Results of sequential fractionation indicates that relatively high proportion $(\~44\%)$ of Cu is associated with organic matter A large proportion of Pb is associated with three types of chemical forms: Fe-Mn oxides, organic matter, and residual. There were significant correlation in concentrations between the exchangeable components and total organic carbons. The heavy metals in the residual phase cannot be easily released to the environment since these are bound to the crystal lattice. But, reducible and organic Phases cfn a significant amount of most heavy metals. Therefore. there is potential danger of a substantial amount of metals becoming chemically mobile with environmental changes.

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Study on the chemical forms of heavy metals in the surface sediments of Ulsan Bay

  • Young Bok Kim;Sun
    • Journal of Environmental Science International
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    • v.2 no.1
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    • pp.69-78
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    • 1993
  • Contents of heavy metals (Cr, Cu, Zn, Cd, and Pb) in the sediments of Ulsan Bay were investigated by the sequential extraction methods that classifies heavy metals into five types of chemical forms: exchangeable, bound to carbonates, bound to Fe-Mn oxides, bound to organic matter and residual. The analyses were determined by using an ICP-MS. Total Cr concentrations in the sediments were in the range of 41.6-96.4, Cu 60.7-680, Zn 189-1954, Cd 33.1-83.4, and Pb 138-567 mg/kg. Results of sequential fractionation indicates that relatively high proportion ( ~44%) of Cu is associated with organic matter. A large proportion of Pb is associated with three types of chemical forms: Fe-Mn oxides, organic matter, and residual. There were significant correlation in concentrations between the exchangeable components and total organic carbons. The heavy metals In the residual phase cannot be easily released to the environment since these are bound to the crystal lattice. But, reducible and organic Phases con a significant amount of most heavy metals. Therefore, there is Potential dancer of a substantial amount of metals becoming chemically mobile with environmental changes.

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Heavy Metal Concentrations in the Mollusc Gastropod, Cipangopaludina chinensis malleata from Upo Wetland Reflect the Level of Heavy Metals in the Sediments

  • Kim, Heung-Tae;Kim, Jae-Geun
    • Journal of Ecology and Environment
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    • v.29 no.5
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    • pp.453-460
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    • 2006
  • Upo wetland is the largest inland wetland in Korea as Ramsar Convention Area. The purposes of the study were to investigate the levels of heavy metals (Cd, Cr, Cu, Ni, Pb and Zn) in the sediment and Cipangopaludina chinensis malleata from three sites of the wetland and to assess the potential of the gastropod as a bioindicator for heavy metal levels. The gastropods were dissected into shell and soft tissue without the digestive and excretive organs. The levels of Cd, Cu and Pb were below the guideline of Soil Environment Conservation Act and the heavy metals except Cr were slightly different among the sites. Cd was higher in Upo site ($0.32{\mu}g/g$) than Sajipo site ($0.28{\mu}g/g$). Cu and Zn showed the highest value in Sajipo as $43.5{\mu}g/g\;and\;39.8{\mu}g/g$, respectively while the concentrations of Pb and Zn were the highest in Upstream as $58.8{\mu}g/g\;and\;138{\mu}g/g$, respectively. In the soft tissues and shells of the gastropod, the overall common trend in the concentrations of the heavy metals was revealed with the following order: Zn > Cu > Cr > Ni > Pb > Cd and Ni > Zn > Cr > Cu > Pb > Cd, respectively. Although the soft tissues exhibited higher concentrations of the heavy metals except Ni than the shell in the gastropod, the levels of Cd and Pb in the gastropod were generally below the restrictive values set up by Korea Food & Drug Administration. From Duncan's Multiple Range Test (DMRT) results, the concentrations of Pb and Zn in the sediments among the sites were reflected on the soft tissue (Pb) and the shell (Pb and Zn) of the gastropod in the same order. The lower value of coefficient of variation (CV) in Pb concentration of the shell than in that of the soft tissue supports the usefulness of the shell as a bioindicator for Pb pollution. Although the CV value in the shell was a little higher than in the soft tissue, DMRT results and the stability of incorporated Zn into the shell support the use of the shell of the gastropod as a potential bioindicator for long-term contamination of Zn.

Improving English communicative competence through individualized WBI (WBI 수준별 수업을 통한 영어 의사소통능력 향상 방안)

  • Jeon, Young-Joo;Kahng, Yong-Koo;Sohng, Hae-Sung
    • English Language & Literature Teaching
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    • v.12 no.4
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    • pp.117-138
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    • 2006
  • The purpose of this study is to increase students' interest in the subject of English, which has been led by some mannerism, and to improve the students' basic scholastic ability and self-controlled problem solving, through adopting an individualized Web Based Instruction(WBI) as a concrete and practical learning method. In order to achieve this, an individualized Web Based Instruction(WBI) homepage has been developed to be applied experimentally to the field of middle school education for 8 months, and a research has been made on its effects on the students' interest in English along with the improvement of English. Two groups which showed almost the same interest were selected as a controlled group and an experimental one. Individualized Web Based Instruction was applied to the experimental group, while the controlled group was not instructed according to student ability. The controlled group was taught using CD added with the textbook. As a result of T-test, the students in the experimental group showed more improvement in communicative competence than those in the controlled group.

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Implementation of a self-mixing type LDF probe and blood flow simulator (자기혼합형 LDF 프로브와 혈류 시뮬레이터의 구현)

  • Ko, Han-Woo;Kim, Jong-Weon
    • Journal of Sensor Science and Technology
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    • v.8 no.2
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    • pp.133-138
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    • 1999
  • In this pager, the authors have implemented a blood flow simulator and a LDF(laser Doppler flowmeter) probe using self-mixing effect of the laser diode. The purpose of the blood flow simulator is to simulate microvascular blood flow in tissue. It consists of melinex film (thickness = $123\;{\mu}m$) which has similar optical characteristics to epidermis and porous polyethylene filter (Vyon, porosity 35%, mean pore size $50\;{\mu}m$, thickness=1 mm) which has similar optical characteristics to dermis. The blood flowmeter probe consists of laser diode(5 mW, 780 nm wavelength), CD lens(focal length 12 mm). current-to-voltage converter, highpass filter, and premplifier. It doesn't need optical fiber, therefore, implementation of the probe is simpler than conventional probe using optical fiber.

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A Study on the Characteristic Analysis of Blue OLED for the Luminous Traffic Safety Mark (발광형 교통안전표지용 청색 OLED의 특성분석에 관한 연구)

  • Kang, Myung-Goo;Kim, Jung-Yeoun;Oh, Hwan-Sool
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.6 no.2
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    • pp.138-145
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    • 2007
  • Luminous traffic safety mark is restricted to use only the place that has a thick fog, many night traffic accidents, limited field of view due to structure of road. Recently, LEDs are used for luminous traffic safety mark, but we propose an organic LED for a novel luminous traffic safety mark in the near future. The device structure was $ITO/2-TNATA(500{\AA})/{\alpha}-NPD(200{\AA})/DPVBi(300{\AA})/BCP(10{\AA})/Alq_3(200{\AA})/LiF(10{\AA})/Al:Li(1000{\AA})$. The characteristics of the device are most efficient on occasion of using $N_2$ gas plasma treatment. Current density is $240.71mA/cm^2$ luminance $10,550cd/m^2$, and current efficiency 3.53cd/A at an applied voltage of 10V. The maximum EL wavelength of the fabricated blue organic light-emitting device is 456nm. CIE color coordinates are x=0.1449 and y=0.1633, which is similar to NTSC deep-blue color with CIE color coordinates of x=0.14 and y=0.08.

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Effect of SipJeonDaeBo-Decoction on Blood Metal Level in Rats (십전대보탕(十全大補湯)을 투여한 흰쥐의 혈액중 금속농도변화에 관한 연구)

  • Joh Hoo-Li;Lee Sun-Dong
    • Journal of Society of Preventive Korean Medicine
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    • v.3 no.2
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    • pp.151-170
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    • 1999
  • This study was to investigate the metal accumulation from SipJeonDaeBo-Decoction to rat blood of Sprague Dawley. 1. There were no significance in body weight, water dose feed ingestion quantity, liver, kidney, brain, bone weights between the control and the experimental groups. Under the experiment with drinking waters was no metal ${\sim}\;0.65mg/L$ detected. Metal level within feed found 0.0001-376.983mg/kg. 2. In the pack of SipJeonDaeBo-decoction, there detected no metal ${\sim}2.086mg/L$ 3. After P.O(per os) SipJeonDaeBo-decoction, As is detected $2.390{\pm}0.812mg/kg$ in blood; Cd $0.001{\pm}0.001mg/kg$, Co $0.003{\pm}0.001mg/kg$, Cr $0.432{\pm}0.234mg/kg$, Cu $1.013{\pm}0.373mg/kg$, Fe $426.293{\pm}114.842mg/kg$, no Hg, Mn $0.109{\pm}0.082mg/kg$, Ni $0.122{\pm}0.068mg/kg$, Zn $3.584{\pm}1.270mg/kg$. 4. The concentration of Hazardous heavy metal (As, Cd, Co, Cr, Hg, Ni, Pb) within blood control group is searched $0.488{\pm}0.138\;mg/l$; experiment I group $0.432{\pm}0.080mg/l$, experiment II group $0.588{\pm}0.213mg/l$. In the concentration of non hazardous heavy metal(Cu, Fe, Mn, Zn) control group $101.409{\pm}6.832mg/l$; experiment I group $96.062{\pm}5.732\;mg/l$, experiment II group $125.139{\pm}044.820mg/l$. 5. Correlation among every metal in blood Zn and Cr was 0.87956 ; Cd and As -0.02316, Pb and As -0.08738, Ni and As 0.07824, Mn and As 0.07824, Mn and Cd 0.04999. Briefly under the injection of SipJeonDaeBo-decoction, this study was defined within safety in blood level by P.O. during 10 days.

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