• Title/Summary/Keyword: CBH

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Plus Tree Selection of Black Locust (Robinia pseudoacacia L.) for Tree Improvement of Timber Characteristics (국내 아까시나무의 용재 특성 향상을 위한 우량개체 선발)

  • Tae-Lim Kim;Hyemin Lim;Kyungmi Lee;Changyoung Oh;Il Hwan Lee;Hwan Su Hwang
    • Korean Journal of Plant Resources
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    • v.36 no.1
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    • pp.91-99
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    • 2023
  • Plus tree selection is the essential first step in a tree improvement program. This study was carried out to select plus trees of black locust (Robinia pseudoacacia L.) for tree improvement in Korea. The candidate plus trees of black locust were selected from twenty-one places in the four provinces (Gangwon, Gyeonggi, Chungcheong and Gyeongsang) following comparison selection. The trees were examined for qualitative and quantitative traits of economic interest like tree height (H), diameter at breast height (DBH), clear bole height (CBH), stem straightness (SS), crown size (CS) and disease resistance. The 64 selected trees showed higher quality in growth characteristics than control trees. Average of height, DBH, CBH, SS and CS were 20.5 m, 31.1 cm, 10.1 m, 1.2 and 5.2 m respectively. In particular, it was shown that the growth characteristics were higher in Chungcheong population. The superiority percentage of selected candidate plus trees of black locust over their respective check trees ranged from 7.3% to 18.9% in height, 20.0% to 40.6% in DBH, 32.8% to 73.5% in CBH, 42.9% to 55.2% in SS and -11.1% to 9.4% in CS, respectively. The selected black locust will be useful in future tree breeding programs based on their clonal and progeny performance.

Improved Homologous Expression of the Acidic Lipase from Aspergillus niger

  • Zhu, Si-Yuan;Xu, Yan;Yu, Xiao-Wei
    • Journal of Microbiology and Biotechnology
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    • v.30 no.2
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    • pp.196-205
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    • 2020
  • In this study, the acidic lipase from Aspergillus niger (ANL) was homologously expressed in A. niger. The expression of ANL was significantly improved by the expression of the native ANL with the introns, the addition of the Kozak sequence and the optimization of the signal sequences. When the cDNA sequence of ANL fused with the glaA signal was expressed under the gpdA promoter in A. niger, no lipase activity could be detected. We then tried to improve the expression by using the full-length ANL gene containing three introns, and the lipase activity in the supernatant reached 75.80 U/ml, probably as a result of a more stable mRNA structure. The expression was further improved to 100.60 U/ml by introducing a Kozak sequence around the start codon due to a higher translation efficiency. Finally, the effects of three signal sequences including the cbhI signal, the ANL signal and the glaA signal on the lipase expression were evaluated. The transformant with the cbhI signal showed the highest lipase activity (314.67 U/ml), which was 1.90-fold and 3.13-fold higher than those with the ANL signal and the glaA signal, respectively. The acidic lipase was characterized and its highest activity was detected at pH 3.0 and a temperature of 45℃. These results provided promising strategies for the production of the acidic lipase from A. niger.

Dielectric Properties in Bi2O3-PbO-SiO2 Glass containing B2O3 (B2O3를 함유한 Bi2O3-PbO-SiO2계 유리의 유전적 특성)

  • Joung, Maeng-Sig;Lee, Su-Dae
    • Journal of Korean Ophthalmic Optics Society
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    • v.6 no.2
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    • pp.23-29
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    • 2001
  • In the SP series of glasses, the formation of droplet is well done with increasing PbO. But in the case SP-1, droplet could not be observed in low concentration PbO. These results are considered that PbO does the role of a network modifier, so the crosslinking break down by $SiO_2$. But, in the glasses contained PbO above 50 mol%, glasses are formeddue to $Pb^{4+}$ ion does the role of a network former. As a result of electrical conductivity analysis for SP series of glasses, the electrical conduction is due to bipolaron at high temperature, and it is due to single polaron hopping at low temperature. In the SP series of glasses, electrical conduction mechanism coincide with the correlated-barrier hopping(CBH) model.

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Enzymatic Hydrolysis of Rice Straw, a Lignocellulosic Biomass, by Extracellular Enzymes from Fomitopsis palustris (Fomitopsis palustris의 균체 외 효소에 의한 볏짚 당화에 관한 연구)

  • Kim, Yoon-Hee;Cho, Moon-Jung;Shin, Keum;Kim, Tae-Jong;Kim, Nam-Hun;Kim, Yeong-Suk
    • Journal of the Korean Wood Science and Technology
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    • v.38 no.3
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    • pp.262-273
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    • 2010
  • In the enzymatic hydrolysis of rice straw and wood meals using extra-cellular enzymes from Fomitopsis palustris, key factors which enhanced the sugar conversion yield were investigated in this work, such as enzyme production and enzyme reaction conditions, surfactant effects, and the surface structure of substrates. F. palustris cultured with softwood mixture produced 12.0 U/$m{\ell}$ for endo-${\beta}$-1,4-gulcanase (EG), 116.68 U/$m{\ell}$ for ${\beta}$-glucosidase (BGL), 18.82 U/$m{\ell}$ for cellobiohydrolase (CBH), and 13.33 U/$m{\ell}$ for ${\beta}$-xylosidase (BXL). These levels of BGL, CBH, and BXL activities were two to four folds more than enzyme activities of F. palustris cultured with rice straw. The optimum reaction conditions of cellulase-RS which produced by F. palustris with rice straw and cellulase-SW which produced by F. palustris with softwood mixture were pH 5.0 at $45^{\circ}C$ and pH 5.0 at $50^{\circ}C$, respectively. The sugar conversion yield of cellulase-SW had the highest value of $40.6{\pm}0.6%$ within 72 h when rice straw was used as substrate. By adding 0.1% Tween 20 (w/w-substrate), the sugar conversion yield of rice straw was increased to 44%, which was about four fifths sugar conversion yield of commercial enzyme, Celluclast 1.5L (Novozyme A/S). A low crystallinity and an intensive fibril surface observed by the scanning electron microscope may explain the high sugar conversion yield of rice straw.

Electrical and Microwave properties of Amorphous As-Ge-Te devices (비정질 As-Ge-Te 스위칭 소자의 전기적 및 마이크로파 주파수 특성)

  • Yi, Byeong-Seok;Cheon, Seok-Pyo;Lee, Hyun-Yong;Lee, Young-Jong;Chung, Hong-Bay
    • Proceedings of the KIEE Conference
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    • 1995.07c
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    • pp.1016-1018
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    • 1995
  • In this paper, we studied the electrical and the microwave properties of the amorphous $As_{10}Ge_{15}Te_{75}$ thin film. The electrical properties of a-$As_{10}Ge_{15}Te_{75}$ thin film were examined d.c. and a.c. bias with annealing condition. As the result of the electrical properties, we observed the physical characteristics of a-$As_{10}Ge_{15}Te_{75}$ thin film such as the density of defect states, characteristic relaxation time, localized density of states, and localized wave function by using CBH and QMT model. We also examined the microwave conduction properties before and after d.e. switching.

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Enzymatic saccharification of rice straw, a lignocellulosic biomass by the extracellular enzyme from KMU001 (KMU001 조효소에 의한 목질계 바이오매스, 볏짚의 효소당화성에 관한 연구)

  • Kim, Yeong-Suk;Lee, Young-Min;Cha, Chang-Jun;Yoon, Jeoung-Jun
    • 한국신재생에너지학회:학술대회논문집
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    • 2008.05a
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    • pp.225-228
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    • 2008
  • This study shows that lignocellulosic biomass saccharification work has been carried out with rice-straw by the extracellular enzyme from KMU001, and the enzymes produced in 5%(w/v) wood biomass were characterized by protein and various enzyme activity measurements. Several cellulases such as Endoglucanase(EG), $\beta$-D-1,4-Glucosidase(BGL), Cellobiohydrolase(CBH), and $\beta$-D-1,4-Xylanase (BXL) were detected. Saccharification of rice-straw by the enzyme yielded about 233mg/g of glucose after 48hrs.

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Enzymatic sccharification of lignocellulosic biomass by enzyme system of brown-rot fungi (갈색부후균의 효소시스템을 이용한 목질계 바이오매스의 효소당화)

  • Yoon, Jeong-Jun;Cha, Chang-Jun;Kim, Yeong-Suk;Kim, Young-Kyoon
    • 한국신재생에너지학회:학술대회논문집
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    • 2006.11a
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    • pp.529-532
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    • 2006
  • Recently the production of ethanol from lignocecllulosics has received much attention due to immense potential for conversion of renewable biometerials into biofuels and chemicals. Fomitopsis palustris causes a typycal brown-rot and is unusual in that it rapidly depolymerize the cellulose in wood without removing the surrounding lignin that normally prevents microbial attack. This study demonstrated that the brown rot basidiomycete F. palustris was able to degrade crystalline cellulose. This fungus could also produce the three major cellulases (BGL, EXG and EG) when the cells were grown on 2.0% Avicel. The fungus was able to degrade both the crystalline and amorphous forms of cellulose from woody biomasses. Moreover, we found that this fungus has the processive EG like CBH which are able to degrade the crystalline region of cellulose. To establish the cellulase system in relation with degradation of woody biomass, we performed that purification, characterization and molecular cloning of a BGL, EGs and GLA from F. palustris grown on Avicel.

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An Efficient Center-Biased Hybrid Search Algorithm (효율적인 Center-Biased Hybrid 탐색 알고리즘)

  • Su-Bong Hong;Soo-Mok Jung
    • Journal of the Korea Computer Industry Society
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    • v.4 no.12
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    • pp.1075-1082
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    • 2003
  • In this paper, we propose an Efficient Center-Biased Hybrid Seearch (ECBHS) for motion estimation based on Center-Biased Hybrid Search(CBHS). This proposed algorithm employ hybrid of a compact plus shaped search, X shaped search, and diamond search to reduce the search point for motion vectors which distributed within 3pels radius of center of search window. ECBHS reduces the computations for motion estimation of CBHS with similar accuracy The efficiency of the proposed algorithm was verified by experimental results.

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A Study of Index Method Based on Main Memory (메모리 기반의 인덱스 기법에 관한 연구)

  • Hong, G.C.;Moon, B.J.
    • Electronics and Telecommunications Trends
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    • v.16 no.6 s.72
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    • pp.29-40
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    • 2001
  • 본 고에서는 디스크 기반의 정보검색시스템의 성능을 높이는 것을 목표로, 주기억장치 상주형 정보검색시스템에 적합한 주기억장치 기반의 인덱싱 기법을 비교 평가하고자 한다. 인덱스는 인덱스를 구성하는 키의 순서가 유지되는지의 여부에 따라 크게 두 종류로 나눌 수 있는데, 키가 일정한 순서로 유지되는 트리 계열과 키의 순서와 관계없이 무작위로 유지되는 해시 계열로 구분할 수 있다. 트리 계열 인덱스는 일정한 범위가 주어지는 연산을 처리할 때 유용하게 사용될 수 있으며, 해시 계열 인덱스는 특정한 키에 의한 빠른 데이터 접근을 제공한다. 트리 계열 인덱스로는 AVL 트리, B+ 트리, T 트리 등이 있으며, 해시 계열 인덱스로는 체인 버켓 해싱(Chained Bucket Hashing: CBH), 확장 해싱(Extendible Hashing: EH), 선형 해싱(Linear Hashing: LH), 수정된 선형 해싱(Modified Linear Hashing), 다중 디렉토리 해싱(Multi-directory Hashing) 및 확장된 체인 버켓 해싱(Extendible Chained Bucket Hashing: ECBH) 등이 있다.