• Korean journal of applied entomology
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• v.53 no.1
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• pp.73-80
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• 2014

Postharvest insect pest control is necessary for agricultural industry including domestic consumer markets and exporting products to meet quarantine issue. Especially, the organic or environmentally friendly agricultural products do not fit to the traditional chemical postharvest treatments mostly using methyl bromide. As an alternative, a physical treatment called CATTS (controlled atmosphere and temperature treatment) has been developed to control various insect and mite pests on apple and several stone fruits. This study was designed to determine the CATTS conditions to control the oriental fruit moth, Grapholita molesta, which is restricted in exporting the infested apples. To apply CATTS on this insect pest, the most heat-tolerant stage was determined. Among the immature stages locating on the fruits, the fifth instar larvae were the most tolerant to $44^{\circ}C$ for 20 min. The ramping rate (the time to increase chamber temperature from $25^{\circ}C$ to $46^{\circ}C$) was positively correlated with the CATTS efficiency under 15% $CO_2$ and 1% $O_2$. After the ramping step, the duration of CATTS was positively correlated with CATTS efficiency. In addition, the CATTS efficiency was highly dependent on the fruit internal temperature at $44^{\circ}C$. From all these parameters, we developed a standard protocol yielding 100% control efficiency of CATTS against apples infested by 4,378 larvae including 2,104 fifth instar individuals.

• Korean journal of applied entomology
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• v.49 no.4
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• pp.363-369
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• 2010

A postharvest treatment called CATTS (controlled atmosphere and temperature treatment system) has been used as an alternative nonchemical measure for methyl bromide fumigant treatment. This study applied CATTS to control the red flour beetle, Tribolium castaneum, infesting stored grains. Adults of T. castaneum were susceptible to $46^{\circ}C$ heat treatment. The susceptibility was further enhanced by addition of CA conditions (15% $CO_2$ and 1% $O_2$). When CATTS ($46^{\circ}C$, 15% $CO_2$, $16^{\circ}C/h$ treating rate) was applied to different developmental stages of T. castaneum, it showed 100% control efficacy by 120 min exposure. There was a variation in CATTS susceptibility among developmental stages, in which late instar larvae were most tolerant. Heat shock proteins of T. castaneum appeared to be implicated in the tolerance of CATTS.

• Korean journal of applied entomology
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• v.49 no.1
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• pp.37-42
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• 2010

As an environment-friendly phytosanitary measure, CATTS (controlled atmosphere temperature treatment system) has been developed to kill several quarantine insect pests infesting subtropical agricultural commodities. This study tested any possibility to apply CATTS to apples to effectively eliminate the peach fruit moth, Carposina sasakii, which has been regarded as a quarantine insect from the imported countries. When the larvae of C. sasakii were directly exposed to $46^{\circ}C$ (an installed lethal temperature of CATTS), they showed a median lethal time at 14.66 min. Addition of high carbon dioxide to the temperature treatment enhanced the thermal limit susceptibility of C. sasakii to $46^{\circ}C$. CATTS device was constructed to automatically control $CO_2$ concentration and temperature with real-time monitoring both in the chamber and in the fruit. The larvae internally infesting apples were tested using the CATTS device and showed 100% lethality after 60 min exposure to a treatment of $46^{\circ}C$ under 15% $CO_2$ in the chamber. Relatively long exposure may be due to the deviation between the ramping temperature ($0.35^{\circ}C$/min) of the chamber and the ramping temperature (0.12-$0.23^{\circ}C$/min) inside apple fruit, where the tested larvae were located. This study suggests a possibility that CATTS can be applied as a quarantine measure to kill the larvae of C. sasakii locating inside the apples.

• Korean journal of applied entomology
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• v.54 no.1
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• pp.17-23
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• 2015

A complete control on quarantine insect pests is required for exporting domestic apples to other countries. To this end, a controlled atmosphere and heat treatment system (CATTS) has been developed as a post-harvest treatment. This study determined the CATTS conditions to control completely two lepidopteran insect pests, the smaller tea tortrix moth, Adoxophyes paraorana and the peach pyralid moth, Dichocrocis punctiferalis, which exhibit different feeding behviors. In both species, the fifth instar larvae were the most tolerant developmental stage to the heat treatment. Under CATTS conditions with 15% $CO_2$ and 1% $O_2$ for 1 h heat treatment at $46^{\circ}C$, the fifth instar larvae of A. paraorana exhibited a complete lethality, while those of D. punctiferalis underwent 88% mortality. To control completely the fifth instar larvae of D. punctiferalis, 2 h heat treatment required under the same atmosphere condition. These CATTS treatment effects were confirmed against over 3,000 fifth instar larvae of each species infesting apples. This study demonstrates that the longer exposure to CATTS conditions is required for the complete disinfestation of the internal apple feeder compared to the non-internal apple feeder.

• Korean journal of applied entomology
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• v.51 no.2
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• pp.131-140
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• 2012

The hawthorn spider mite, Tetranychus viennensis, is a pest of apples and a quarantine pest from some countries that import apples from Korea. A controlled atmosphere and temperature treatment system (CATTS) was developed as an alternative disinfestation method to methyl bromide fumigation treatment, and has been applied to control various insects and other arthropod pests on fruits. We applied CATTS to disinfect T. viennensis under conditions that were previously developed to control the peach fruit moth, Carposina sasakii. First, T. viennensis was sampled from Japanese apricot, Prunus mume, and identified by its morphological characters. In addition, both cytochrome oxidase I (COI) and internal transcribed spacer (ITS) sequences supported the morphological identification. Second, the heat-tolerant developmental stage was determined in T. viennensis. When a $46^{\circ}C$ heat treatment was applied to egg, nymph, and adult stages of T. viennensis, adults were the most tolerant stage. Third, when heat temperature was used along with 1% $O_2$ and 15% $CO_2$, the mites showed a significant increase in susceptibility to the heat treatment. Finally, CATTS at $46^{\circ}C$ with 15% $CO_2$ and 1% $O_2$ for 30 min resulted in 100% mortality of all T. viennensis development stages. These results indicated that CATTS isapplicable to disinfest T. viennensis in post-harvest apples.