• Title/Summary/Keyword: CAT-2

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CROSSED SEMIMODULES AND CAT1-MONOIDS

  • Temel, Sedat
    • Korean Journal of Mathematics
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    • v.27 no.2
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    • pp.535-545
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    • 2019
  • The main idea of this paper is to introduce the notion of $cat^1$-monoids and to prove that the category of crossed semimodules ${\mathcal{C}}=(A,B,{\partial})$ where A is a group is equivalent to the category of $cat^1$-monoids. This is a generalization of the well known equivalence between category of $cat^1$-groups and that of crossed modules over groups.

Molecular Cloning, Segmental Distribution and Ontogenetic Regulation of Cationic Amino Acid Transporter 2 in Pigs

  • Zou, Shi-geng;Zhi, Ai-min;Zhou, Xiang-yan;Zuo, Jian-jun;Zhang, Yan;Huang, Zhi-yi;Xu, Ping-Wen;Feng, Ding-yuan
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.5
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    • pp.712-720
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    • 2009
  • The goal of this study was to elucidate the expression and segmental distribution of the glomerular cationic amino acid metabolism transporter-2 (CAT-2) and thus to improve our understanding of porcine cationic amino acid transporters and amino acid absorption. Porcine CAT-2 was cloned, sequenced and characterized. The predicted amino acid sequence of porcine CAT-2 shared 86.1% and 92.1% identity with human and mouse CAT-2A, respectively. The tissue distribution patterns and ontogenic changes of CAT-2 mRNAs were determined by real-time Q-PCR. The results showed that porcine CAT-2 was highly expressed in the heart and intestinal tract (duodenum, ileum and jejunum). In addition, the mRNA of CAT-2 was found in liver, lung, kidney, brain and muscle. Within the intestinal tract, CAT-2 mRNA was most abundant in the ileum and rarely expressed in the duodenum. In the duodenum, the levels of CAT-2 mRNA reached their peak on day 7 (p<0.05) while in the jejunum, levels were low on day 1 and 7 and increased rapidly after day 26 before peaking on days 30 and 60 (p<0.05). The levels then dramatically decreased by day 90 (p<0.05). In the ileum, levels achieved their maximum on day 30 and then decreased significantly on day 60 (p<0.05).

Effect of Viral Enhancers on the Tissue-Specific Expression of Bovine Growth Hormone Gene (소성장호르몬 유전자의 조직 특이성 발현에 미치는 바이러스 engancer의 영향)

  • 박계윤;김수미;노정혜
    • Korean Journal of Microbiology
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    • v.27 no.2
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    • pp.85-91
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    • 1989
  • The effect of SV40 and murine cytomegalovirus (MCMV) enhancers on the general and tissue-specific gene expression was investigated. Recombinant plasmids containing these transcriptional engancers downstream of a structural gene for chloramphenicol acetyl transferase (CAT) were constructed. The transient expression of CAT gene from these plasmids was measured in monkey (CV1PD) and HeLa cells. Both SV40 and MCMV engancers activated the expression of CAT gene by more than 20 and 150 folds, respectively, compared with engancerless plasmids. When the SV40 promoter, upstream of CAT gene, was replaced with 2.2 kbp of promoter regulatory region of bovine growth hormone (bGH) gene, there was no expression of CAT even in the presence of enhancers, reflecting the tissue-specific expression of bGH genes. However, when the bGH regulatory region was shortened to 230 bp, the expression level increased dramatically in the presence of SV40 enhancers. In contrast, the expression from the shortened promoter was only marginally activated by the stronger MCMV enhancer.

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Sperm hyperactivation and the CatSper channel: current understanding and future contribution of domestic animals

  • Jae Yeon Hwang
    • Journal of Animal Science and Technology
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    • v.66 no.3
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    • pp.443-456
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    • 2024
  • In female tract, mammalian sperm develop hyperactivated motility which is a key physiological event for sperm to fertilize eggs. This motility change is triggered by Ca2+ influx via the sperm-specific Ca2+ channel, CatSper. Although previous studies in human and mice largely contributed to understanding CatSper and Ca2+ signaling for sperm hyperactivation, the differences on their activation mechanisms are not well understood yet. There are several studies to examine expression and significance of the CatSper channel in non-human and non-mouse models, such as domestic animals. In this review, I summarize key knowledge for the CatSper channel from previous studies and propose future aspects for CatSper study using sperm from domestic animals.

Design of XML Using UML in EtherCAT-based Encoder System (EtherCAT 기반 엔코더 시스템에서 UML을 이용한 XML 설계)

  • Lee, Ju-Kyoung;Lee, Suk;Lee, Kyung-Chang
    • IEMEK Journal of Embedded Systems and Applications
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    • v.9 no.2
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    • pp.117-125
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    • 2014
  • The EtherCAT protocol has become a very promising alternative for real-time industrial application due to its ability to eliminate uncertainties in the Ethernet. However, the extended markup language (XML) for the EtherCAT network system, which is required in the design, lacks systematic development to take advantage of model transformation techniques. This paper focuses on the system development procedure of the EtherCAT-based encoder system using the CANopen over EtherCAT (CoE) protocol. UML modeling is being adapted to design for XML of EtherCAT-based encoder system. To this purpose, this paper analyzes the object dictionary (OD) of a commercial encoder and CANopen over EtherCAT. A UML diagram is then designed based on the analysis, and XML is generated through the designed UML diagram. Finally, an experimental test_bed for the EtherCAT-based encoder system is implemented and its performance is compared with a commercial encoder.

Effect of Light and Cadmium on the Activity and Isozyme Pattern of Catalase from Ric(Oryza sativa L.) (빛과 카드뮴이 벼 catalase 활성과 동위효소 발현에 미치는 영향)

  • Kim, Yoon-Kyoung;Lee, Mi-Young
    • Applied Biological Chemistry
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    • v.49 no.4
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    • pp.287-292
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    • 2006
  • The effects of cadmium on the catalase activity and isozyme patterns under light and dark conditions of rice(Oryza sativa L. cv. Dongjin) seedlings were examined. Cadmium treatment resulted in the notable enhancement of $H_2O_2$ contents in the seedling roots and leaves under light and dark conditions. The catalase isozyme patterns in the roots were different from those in the leaves, showing tissue-specific expression of the enzyme. Moreover, the expression patterns of catalase isozymes in the green seedling roots were different from those in the etiolated seedling roots following cadmium treatment. The increase of total catalase activity was about 16 times at 1 mM cadmium and marked inductions of the isozyme CAT1 and CAT2 contributed to this increase in the green seedling roots. On the other hand, in the etiolated seedling roots, total catalase activity was lower than that of control at 0.5 and 1 mM cadmium, even though catalase activity increased about 3 times at 0.1 mM cadmium. The 3 fold increase of total catalase activity was mainly due to the increase of CAT1, CAT3 and CAT4 at 0.1 mM cadmium. However, treatment with higher concentrations of cadmium decreased the activity of CAT2 and CAT4 in the etiolated roots. In the leaves, the catalase existed as three isozymes; one cationic isozyme CATc, one neutral isozyme CATn and one anionic isozyme CAT1 in the control. The isozyme patterns and total activities remained unaffected by cadmium under light and dark conditions in the seedling leaves. Taken together, it seems that cadmium-induced changes of catalase might be regulated by light in the roots, but not in the leaves.

Simultaneous and Sequential Co-Immobilization of Glucose Oxidase and Catalase onto Florisil

  • Gul, Ozyilmaz;Tukel, S. Seyhan
    • Journal of Microbiology and Biotechnology
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    • v.17 no.6
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    • pp.960-967
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    • 2007
  • The co-immobilization of Aspergillus niger glucose oxidase (GOD) with bovine liver catalase (CAT) onto florisil (magnesium silicate-based porous carrier) was investigated to improve the catalytic efficiency of GOD against $H_2O2$ inactivation. The effect of the amount of bound CAT on the GOD activity was also studied for 12 different initial combinations of GOD and CAT, using simultaneous and sequential coupling. The sequentially co-immobilized GOD-CAT showed a higher efficiency than the simultaneously co-immobilized GOD-CAT in terms of the GOD activity and economic costs. The highest activity was shown by the sequentially co-immobilized GOD-CAT when the initial amounts of GOD and CAT were 10 mg and 5 mg per gram of carrier. The optimum pH, buffer concentration, and temperature for GOD activity for the same co-immobilized GOD-CAT sample were then determined as pH 6.5, 50 mM, and $30^{\circ}C$, respectively. When compared with the individually immobilized GOD, the catalytic activity of the co-immobilized GOD-CAT was 70% higher, plus the reusability was more than two-fold. The storage stability of the co-immobilized GOD-CAT was also found to be higher than that of the free form at both $5^{\circ}C\;and\;25^{\circ}C$. The increased GOD activity and reusability resulting from the co-immobilization process may have been due to CAT protecting GOD from inactivation by $H_2O2$ and supplying additional $O_2$ to the reaction system.

IDEAL BOUNDARY OF CAT(0) SPACES

  • Jeon, Myung-Jin
    • Communications of the Korean Mathematical Society
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    • v.13 no.1
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    • pp.95-107
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    • 1998
  • In this paper we prove the Hopf-Rinow theorem for CAT(0) spaces and show that the ideal boundaries of complete CAT(0) manifolds of dimension 2 or 3 with some additional conditions are homeomorphic to the circle or 2-sphere by the characterization of the local shadows around the branch points.

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Production of Transgenic Olive Flounder (Paralichthys olivaceus) I. In vivo Gene Transfer in Olive Flounder by Direct Intramuscular Injection (외래 유전자가 이식된 넙치(Paralichthys olivaceus) 생산 I. 근육내 유전자 직접 주입법을 통한 in vivo 유전자 이식)

  • 남윤권;주수동;정창화;방인철;허성범;김동수
    • Journal of Aquaculture
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    • v.10 no.4
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    • pp.409-415
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    • 1997
  • The carp $\beta$-actin regulatory sequences and RSV/LTR promoter were tested whether they are functinal to express linked structure gene (chloramphenicol acetyltransferas, CAT) in olive flounder (Paralichthys olivaceus) by determining the patterns of gene expression following intramuscular in vivo direct injection. The injection experiments with various concentrations of both pRSVCAT and pFV4CAT clearly revealed the effectiveness of DNA dosage on expression of CAT. The increase of CAT activity was linear in both plasmids, and maximal CAT activity was obtained with 100 ug of pFV4CAT injection. The amounts of CAT expression with pFV4CAT-injected fist were higher than those with pRSVCAT-injected fish. CAT activity was readily detectable as early as one day after injection, slightly increased at day 2, and declined over time. Most amount of DNA intramuscularly injected into olive flounder muscles persisted extrachromosomally without showing any integrated or replicated form in vivo.

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A Study on Implementation of Real-time EtherCAT Master (실시간 EtherCAT 마스터 구현에 관한 연구)

  • Kang, Sung Jin
    • Journal of the Semiconductor & Display Technology
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    • v.20 no.2
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    • pp.131-136
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    • 2021
  • EtherCAT is an Ethernet-based fieldbus system standardized in IEC 61158 and SEMI, and widely used in the fields of factory automation, semiconductor equipment and robotics. In this paper, a real-time EtherCAT master is implemented on Linux operating systems and its performances are evaluated. To enhance the real-time capability of mainline Linux kernel, Xenomai is applied as a real-time framework and an open source EtherCAT master stack, Simple Open EtherCAT Master (SOEM), is installed on it. Unlike other studies, the real-time performance of the EtherCAT master is evaluated at the output of the network interface card, so that the evaluation results include all possible effects from the EtherCAT master system. The implemented EtherCAT master can send and receive packets up to 20KHz control frequency with low jitter, even in stressed condition.