• 제목/요약/키워드: CAS-4

검색결과 289건 처리시간 0.023초

차세대 중형위성용 2축 짐벌식 X-밴드 안테나의 열설계 및 궤도 열해석 (Thermal Design and Analysis for Two-Axis Gimbal-Type X-Band Antenna of Compact Advanced Satellite)

  • 채봉건;유창목;장수영;강은수;오현웅
    • 한국항공우주학회지
    • /
    • 제46권4호
    • /
    • pp.306-314
    • /
    • 2018
  • 차세대 중형위성에 탑재되는 2축 짐벌형 X-밴드 안테나는 대용량 영상정보 송신 안테나로, 위성의 자세와 궤도 운동과 무관하게 자세 및 안테나 지향각에 따라 방위각 축과 고각축이 동시에 회전 구동하여 지상안테나를 지향하게 된다. 상기 안테나는 위성체 외부에 극심한 온도차를 보이는 궤도 열환경에 노출되며, 안테나 및 주요 구성품이 허용온도 범위 내를 유지할 수 있도록 열설계를 해야 한다. 본 논문에서는 2축 짐벌형 X-밴드 안테나의 열설계 유효성을 해석적으로 요구조건을 만족함을 확인하였으며, 시스템 열진공 시험 조건해석 및 궤도 운용조건에서의 열해석을 수행하여 히터 용량 및 듀티 사이클을 도출하였다. 아울러, 상기 안테나의 핵심 주요 구성품에 대한 허용온도 요구조건 충족 여부를 확인하였다.

Butanol 생합성 Clostridium 속 미생물 대사공학용 게놈 편집 도구 개발 (Development of Genome Engineering Tools for Metabolic Engineering of Butanol-producing Clostridium Species)

  • 우지은;김민지;이지원;서효주;이상엽;장유신
    • KSBB Journal
    • /
    • 제31권4호
    • /
    • pp.193-199
    • /
    • 2016
  • Global warming caused from the heavy consumption of fossil fuel is one of the biggest problems to be solved. Biofuel has been gained more attention as an alternative to reduce the consumption of fossil fuel. Recently, butanol produced from the genus Clostridium has been considered as one of the promising alternatives for gasoline, fossil based fuel. Nevertheless, the lack of the genome-engineering tools for the genus Clostridium is the major hurdle for the economic production of butanol. More recently, genome engineering tools have been developed for metabolic engineering of butanol-producing Clostridium species, which includes genome scale network model and genome editing tools on the basis of mobile group II introns and CRISPR/Cas system. In this study, the genome engineering tools for butanol-producing Clostridium species have been reviewed with a brief future perspective.

Study of Spectral Reflectance Reconstruction Based on an Algorithm for Improved Orthogonal Matching Pursuit

  • Leihong, Zhang;Dong, Liang;Dawei, Zhang;Xiumin, Gao;Xiuhua, Ma
    • Journal of the Optical Society of Korea
    • /
    • 제20권4호
    • /
    • pp.515-523
    • /
    • 2016
  • Spectral reflectance is sparse in space, and while the traditional spectral-reconstruction algorithm does not make full use of this characteristic sparseness, the compressive sensing algorithm can make full use of it. In this paper, on the basis of analyzing compressive sensing based on the orthogonal matching pursuit algorithm, a new algorithm based on the Dice matching criterion is proposed. The Dice similarity coefficient is introduced, to calculate the correlation coefficient of the atoms and the residual error, and is used to select the atoms from a library. The accuracy of Spectral reconstruction based on the pseudo-inverse method, Wiener estimation method, OMP algorithm, and DOMP algorithm is compared by simulation on the MATLAB platform and experimental testing. The result is that spectral-reconstruction accuracy based on the DOMP algorithm is higher than for the other three methods. The root-mean-square error and color difference decreases with an increasing number of principal components. The reconstruction error decreases as the number of iterations increases. Spectral reconstruction based on the DOMP algorithm can improve the accuracy of color-information replication effectively, and high-accuracy color-information reproduction can be realized.

정형외과 환자의 수술 후 변비 발생과 영향 요인 (Constipation in Patients following Orthopedic Surgery: Incidence and Influencing Factors)

  • 박정희;윤선옥;김선화;유미경;함은진
    • 성인간호학회지
    • /
    • 제28권6호
    • /
    • pp.637-645
    • /
    • 2016
  • Purpose: The purpose of this study was to identify the occurrences and the factors contributing to constipation in the post surgical period following major orthopedic surgery. Methods: The sample included 133 patients who had surgery of the hip, knee, or spine. Patients were excluded from the study if there was a history of bowel surgery or constipation from chart review. Data were collected using questionnaires such as Korean version of Modified Barthel index (K-MBI), Hospital Anxiety and Depression Scale, and Constipation Assessment Scale (CAS) before surgery and on the third postoperative day. Results: Reports of constipation occurred in 77 instances (57.9%). The first reported defecation was within $4.0{\pm}2.2days$ following surgery. Mean days of first defecation of constipation group vs. non-constipation group was $5.5{\pm}1.7days$ vs. $2.1{\pm}1.0days$. There were statistically significant differences between patients who reported than those who did not in terms of age, length of NPO period, postoperative ADL, and the reported incidents of preoperative and postoperative depressive symptoms. Postoperative ADL, age, and NPO period were significant influencing factors of constipation and explained 52.4% of the variance. Conclusion: Constipation is a very common symptom for patients undergoing orthopedic surgery. These results indicate a need for improving patient's ADL after surgery to prevent constipation.

Effect of BIS depletion on HSF1-dependent transcriptional activation in A549 non-small cell lung cancer cells

  • Yun, Hye Hyeon;Baek, Ji-Ye;Seo, Gwanwoo;Kim, Yong Sam;Ko, Jeong-Heon;Lee, Jeong-Hwa
    • The Korean Journal of Physiology and Pharmacology
    • /
    • 제22권4호
    • /
    • pp.457-465
    • /
    • 2018
  • The expression of BCL-2 interacting cell death suppressor (BIS), an anti-stress or anti-apoptotic protein, has been shown to be regulated at the transcriptional level by heat shock factor 1 (HSF1) upon various stresses. Recently, HSF1 was also shown to bind to BIS, but the significance of these protein-protein interactions on HSF1 activity has not been fully defined. In the present study, we observed that complete depletion of BIS using a CRISPR/Cas9 system in A549 non-small cell lung cancer did not affect the induction of heat shock protein (HSP) 70 and HSP27 mRNAs under various stress conditions such as heat shock, proteotoxic stress, and oxidative stress. The lack of a functional association of BIS with HSF1 activity was also demonstrated by transient downregulation of BIS by siRNA in A549 and U87 glioblastoma cells. Endogenous BIS mRNA levels were significantly suppressed in BIS knockout (KO) A549 cells compared to BIS wild type (WT) A549 cells at the constitutive and inducible levels. The promoter activities of BIS and HSP70 as well as the degradation rate of BIS mRNA were not influenced by depletion of BIS. In addition, the expression levels of the mutant BIS construct, in which 14 bp were deleted as in BIS-KO A549 cells, were not different from those of the WT BIS construct, indicating that mRNA stability was not the mechanism for autoregulation of BIS. Our results suggested that BIS was not required for HSF1 activity, but was required for its own expression, which involved an HSF1-independent pathway.

Enhanced Production of Soluble Pyrococcus furiosus α-Amylase in Bacillus subtilis through Chaperone Co-Expression, Heat Treatment and Fermentation Optimization

  • Zhang, Kang;Tan, Ruiting;Yao, Dongbang;Su, Lingqia;Xia, Yongmei;Wu, Jing
    • Journal of Microbiology and Biotechnology
    • /
    • 제31권4호
    • /
    • pp.570-583
    • /
    • 2021
  • Pyrococcus furiosus α-amylase can hydrolyze α-1,4 linkages in starch and related carbohydrates under hyperthermophilic condition (~ 100℃), showing great potential in a wide range of industrial applications, while its relatively low productivity from heterologous hosts has limited the industrial applications. Bacillus subtilis, a gram-positive bacterium, has been widely used in industrial production for its non-pathogenic and powerful secretory characteristics. This study was conducted to increase production of P. furiosus α-amylase in B. subtilis through three strategies. Initial experiments showed that co-expression of P. furiosus molecular chaperone peptidyl-prolyl cis-trans isomerase through genomic integration mode, using a CRISPR/Cas9 system, increased soluble amylase production. Therefore, considering that native P. furiosus α-amylase is produced within a hyperthermophilic environment and is highly thermostable, heat treatment of intact culture at 90℃ for 15 min was performed, thereby greatly increasing soluble amylase production. After optimization of the culture conditions (nitrogen source, carbon source, metal ion, temperature and pH), experiments in a 3-L fermenter yielded a soluble activity of 3,806.7 U/ml, which was 3.3- and 28.2-fold those of a control without heat treatment (1,155.1 U/ml) and an empty expression vector control (135.1 U/ml), respectively. This represents the highest P. furiosus α-amylase production reported to date and should promote innovation in the starch liquefaction process and related industrial productions. Meanwhile, heat treatment, which may promote folding of aggregated P. furiosus α-amylase into a soluble, active form through the transfer of kinetic energy, may be of general benefit when producing proteins from thermophilic archaea.

IPTV Flexible-PPC 환경에서의 안전한 콘텐츠 접근 제어 시스템 (Secure Contents Access Control System in IPTV Flexible-PPC Model)

  • 강용구;임지환;오희국
    • 정보보호학회논문지
    • /
    • 제21권1호
    • /
    • pp.93-104
    • /
    • 2011
  • 제한수신시스템은 IPTV 서비스의 가입자에게 비용을 청구하고 인가된 사용자에게만 서비스를 제공하기 위한 필수적인 요소이다. 현재 IPTV는 PPC(pay-per-channel)와 PPV(pay-per-view) 두 가지 환경으로 서비스되고 있는데, 사용자의 취향에 따라 임의의 채널을 조합할 수 있고 구독기간에 관계없이 개별적인 채널 추가 및 해지가 가능한 F-PPC(Flexible-PPC) 환경이 요구된다. 기존에 제안된 제한수신시스템 기법들을 F-PPC 환경에 적용하기에는 문제점이 존재한다. 본 논문에서는 F-PPC 환경에 적용할 수 있는 4계층 키 구조의 제한수신시스템을 제안한다. 제안하는 시스템은 이진트리를 이용하여 효율적인 키 관리가 가능하고 키 갱신에 요구되는 메시지 통신량이 O(1) 수준이기 때문에 대역폭의 부담이 적다. 따라서 기존의 제한수신시스템에 비해 효율적이고 안전하다.

Stage by stage design for primary, conventional activated sludge, SBR and MBBR units for residential wastewater treatment and reusing

  • Aziz, Shuokr Qarani;Omar, Imad Ali;Bashir, Mohammed J.K.;Mojiri, Amin
    • Advances in environmental research
    • /
    • 제9권4호
    • /
    • pp.233-249
    • /
    • 2020
  • To date, there is no central wastewater (WW) treatment plant in Erbil city, Kurdistan region, Iraq. Therefore, raw WW disposes to the environment and sometimes it used directly for irrigation in some areas of Erbil city. Disposal of the untreated WW to the natural environment and using for irrigation it causes problems for the people and the environment. The aims of the current work were to study the characteristics, design of primary and different secondary treatment units and reusing of produced WW. Raw WW samples from Ashty city-Erbil city were collected and analyzed for twenty three quality parameters such as Total Suspended Solids (TSS), total dissolved solids, total volatile and non-volatile solids, total acidity, total alkalinity, total hardness, five-day Biochemical Oxygen Demand (BOD5), Chemical Oxygen Demand (COD), biodegradability ratio (BOD5/COD), turbidity, etc. Results revealed that some parameters such as BOD5 and TSS were exceeded the standards for disposal of WW. Design and calculations for primary and secondary treatment (biological treatment) processes were presented. Primary treatment units such as screening, grit chamber, and flow equalization tank were designed and detailed calculation were illustrated. While, Conventional Activated Sludge (CAS), Sequencing Batch Reactor (SBR) and Moving Bed Biofilm Reactors (MBBR) were applied for the biological treatment of WW. Results revealed that MBBR was the best and economic technique for the biological treatment of WW. Treated WW is suitable for reusing and there is no restriction on use for irrigation of green areas inside Ashty city campus.

Spatial Gap-Filling of Hourly AOD Data from Himawari-8 Satellite Using DCT (Discrete Cosine Transform) and FMM (Fast Marching Method)

  • Youn, Youjeong;Kim, Seoyeon;Jeong, Yemin;Cho, Subin;Kang, Jonggu;Kim, Geunah;Lee, Yangwon
    • 대한원격탐사학회지
    • /
    • 제37권4호
    • /
    • pp.777-788
    • /
    • 2021
  • Since aerosol has a relatively short duration and significant spatial variation, satellite observations become more important for the spatially and temporally continuous quantification of aerosol. However, optical remote sensing has the disadvantage that it cannot detect AOD (Aerosol Optical Depth) for the regions covered by clouds or the regions with extremely high concentrations. Such missing values can increase the data uncertainty in the analyses of the Earth's environment. This paper presents a spatial gap-filling framework using a univariate statistical method such as DCT-PLS (Discrete Cosine Transform-based Penalized Least Square Regression) and FMM (Fast Matching Method) inpainting. We conducted a feasibility test for the hourly AOD product from AHI (Advanced Himawari Imager) between January 1 and December 31, 2019, and compared the accuracy statistics of the two spatial gap-filling methods. When the null-pixel area is not very large (null-pixel ratio < 0.6), the validation statistics of DCT-PLS and FMM techniques showed high accuracy of CC=0.988 (MAE=0.020) and CC=0.980 (MAE=0.028), respectively. Together with the AI-based gap-filling method using extra explanatory variables, the DCT-PLS and FMM techniques can be tested for the low-resolution images from the AMI (Advanced Meteorological Imager) of GK2A (Geostationary Korea Multi-purpose Satellite 2A), GEMS (Geostationary Environment Monitoring Spectrometer) and GOCI2 (Geostationary Ocean Color Imager) of GK2B (Geostationary Korea Multi-purpose Satellite 2B) and the high-resolution images from the CAS500 (Compact Advanced Satellite) series soon.

고해상도 광학 위성영상의 항만선박관리 활용 가능성 평가: 부산 신항의 선석 활용을 대상으로 (Evaluation of the Utilization Potential of High-Resolution Optical Satellite Images in Port Ship Management: A Case Study on Berth Utilization in Busan New Port)

  • 김현수 ;장소영 ;김태호
    • 대한원격탐사학회지
    • /
    • 제39권5_4호
    • /
    • pp.1173-1183
    • /
    • 2023
  • 한국의 전체 수출입 물동량은 지난 20여년 동안 연평균 약 5.3%씩 증가하였고, 약 99%가량의 화물이 여전히 해상을 통해 운송되고 있는 것으로 나타났다. 최근 해상 물동량 증가, 코로나 및 전쟁 등의 이유로 해상 물류가 혼잡해지고 예측이 어려워지고 있어 지속적인 항만의 모니터링이 중요하다. 다양한 지상 관측 시스템과 automatic identification system (AIS) 정보를 이용하여 항만을 모니터링하고 항만 내 컨테이너 터미널의 효율적 운영과 물동량 예측을 위한 많은 선행 연구가 진행되었다. 하지만, 소형 무역항이나 개발도상국의 무역항의 경우 대형 항만에 비해 환경 문제와 노후화된 인프라 등의 이유로 항만을 모니터링하기에 어려움이 있다. 최근 인공위성의 활용성이 높아짐에 따라 광범위하고 접근하기 어려운 지역에 대해 위성 영상을 이용하여 지속적인 해상 물동량 데이터 수집 및 해양 감시체계 구축을 위한 선행 연구가 진행되고 있다. 본 연구는 고해상도 위성영상을 이용하여 부산 신항을 대상으로 항만 내 컨테이너 터미널에 존재하는 선석에 정박한 선박을 육안으로 탐지하고 선석 활용률을 정량적으로 평가하고자 한다. 국토위성, 아리랑위성 3호, PlanetScope, Sentinel-2A를 이용해 항만 내 선석에 정박하고 있는 선박을 육안으로 탐지하였고 선석에 정박 가능한 전체 선박의 수를 이용하여 선석 활용률을 산출하였다. 산출 결과 2022년 6월 2일의 경우 0.67, 0.7, 0.59로 변화하는 것을 보였으며, 영상 촬영 시각에 따라 선박의 수가 변화한 것으로 확인되었다. 2022년 6월 3일의 경우 0.7로 동일한 것으로 나타났고 이는 선박의 종류는 변화하였으나 촬영 시각에 선박의 수는 동일한 것으로 확인이 되었다. 선석 활용률은 값이 클수록 해당 선석에서의 작업이 활발하게 이루어지고 있는 것을 의미하고 있으며, 이는 선석이 혼잡하여 정박지에서 대기하고 있는 다른 선박의 대기시간이 길어지고 운임료가 증가할 수 있기 때문에 선석 활용률을 이용하여 기초적인 새로운 선박 운항 계획 수립에 도움이 될 것으로 판단된다. 선석에서의 작업시간은 수시간에서 수일이 소요되는데 영상의 촬영 시간 차이에 따른 선석에서의 선박의 변화율을 산출한 결과 4분 49초의 시간차이에도 선박의 변화가 있는 것을 확인할 수 있었다. 이는 관측 주기가 짧고 고해상도 위성영상을 모두 이용한다면 항만내 지속적인 모니터링이 가능할 것으로 사료된다. 그리고 항만 내 선박의 변화를 최소 시간 단위로 확인할 수 있는 위성 영상을 활용하면 항만 관리가 이루어지지 않는 소형 무역항이나 개발도상국의 무역항 등에서도 유용하게 사용할 수 있을 것으로 기대된다.