• 생물정신의학
• /
• 제6권2호
• /
• pp.240-245
• /
• 1999

Object : This study was designed to evaluate the effects of olanzapine on the schedule-induced polydipsia(SIP) which is one of animal model of obsessive-compulsive disorder in rats. We administered olanzapine as a serotonin and dopamine blocking agent, fluoxetine as a selective serotonin reuptake inhibitor, and haloperidol for the dopamine antagonist to rats which showed schedule-induced polydipsic behavior. Methods : Spraque-Dawley rats weighing 200-250gm were individually housed and maintained and allowed free access to water. The rats were placed on a restricted diet. To induce polydipsia, rats were placed in the cage where a pellet dispenser automatically dispensed 90mg pellets on a fixed-time 60 seconds(FT-60s) feeding schedule over 150 minute test session per day. Water was available at all times in the cage. After 4 weeks of daily exposure to the FT 60s feeding schedule, experimental rats met a predetermined criterion for polydipsic behavior(greater than 3 times of water per session on average). 5 groups of rats were administered olanzapine(3mg/kg, i.p), olanzapine(10mg/kg, i.p), fluoxetine(5mg/kg, i.p.), haloperidol(0.1mg/kg, i.p.), and vehicle(1cc/kg, i.p.) for 3 weeks. The rats were tested once a week to access schedule induced polydipsic behavior. Water bottles were weighed before and after the 150-minute test session. The chronic effects of administration of experimental drugs on schedule induced polydipsic behavior were analyzed with ANOVA and Scheffe test as a posthoc comparison. In order to measure water consumption in non-polydipsic food-deprived rats, a separate group of rats(N=8) were individually housed and given a single bolus(14.5gm) of food per day which maintained them at their average body weight. Results and Conclusion : The results were as follows ; 1) After 4 weeks of scheduled feeding procedure, the experimental group showed significant differences than the bolus control in the amount of water consumption as compared with their average water intakes for 4 weeks. At the same periods, there were no differences between the experimental group and the bolus control in the body weight. 2) The fluoxetine group showed significant decrease in the amount of water intake over the 3 weeks of drug treatment as compared with their average amount of polydipsic water intakes. The olanzapine 3mg group showed significant decrease in the amount of water intake at 3rd weeks of drug treatment as compared with their average amount of polydipsic water intakes. The olanzapine 10mg group showed significant decrease in the amount of water intake at 2nd and 3rd weeks of drug treatment as compared with their average amount of polydipsic water intakes. However, the haloperidol group and the vehicle control group showed no changes of amounts of water intake for 3 weeks of treatment as compared with their average amount of polydipsic water intakes. 3) The fluoxetine group showed significantly lower amounts of water intake than the haloperidol group at 2nd weeks of drug treatment. And also the fluoxetine group showed significantly lower amounts of water intake than the haloperidol group and the vehicle control at 3rd weeks of drug treatment. The olanzapine 3mg group and the olanzapine 10mg group showed significantly lower amounts of water intake than the haloperidol group and the vehicle control at 3rd weeks of drug treatment. Above findings suggest that the fixed time feeding procedure for schedule-induced polydipsia as an animal model of obsessive compulsive disorder was effective to the evaluation of pharmacological challenge study. The authors assume that the serotonin hypothesis and the serotonin-dopamine interaction hypothesis are preferred to the dopamine hypothesis in the biological etiology of obsessive-compulsive disorder.

• 한국지반공학회논문집
• /
• 제36권5호
• /
• pp.25-34
• /
• 2020

최근 현장타설말뚝의 네킹 결함을 평가하기 위한 비파괴평가기법으로 전자기파를 적용하는 연구가 활발히 진행되고 있다. 하지만, 전자기파의 전파는 주변 매질의 함수비에 큰 영향을 받는다. 본 연구에서는 서로 다른 함수비의 모래 지반에 설치되어 있는 현장타설말뚝에 발생한 네킹 결함을 전자기파를 이용하여 평가할 수 있는 방법을 실내실험을 통해 고찰하고자 한다. 실내실험을 위해 네킹 결함이 있는 직경 600mm, 길이 1m의 모형 말뚝을 제작하였으며, 건조된 모래와 함수비가 10%, 20%, 30%인 모래에 설치된 조건에서 실험을 수행하였다. 전자기파의 전파를 위해 철근망에 전선을 설치하여 전송선로를 구성하였다. 전자기파의 송신과 수신을 위해 time domain reflectometer를 사용하였다. 실험결과, 모형 말뚝의 두부와 선단부뿐만 아니라 네킹 결함부에서도 전자기파의 반사 신호가 뚜렷이 나타났다. 모래의 함수비가 증가할수록 네킹 결함부에서 반사되는 전자기파 신호의 최대 진폭이 감소하였으며, 모형 말뚝 선단에서 반사된 전자기파의 속도는 네킹 결함 내의 모래의 함수비가 증가할수록 감소하였다. 하지만, 전자기파의 도달시간과 속도를 이용하여 산정된 네킹 결함의 위치와 실제 네킹 결함의 위치가 거의 같았다. 본 연구는 전자기파가 모래 지반에 설치된 현장타설말뚝의 네킹 결함을 평가하는데 유용하게 활용될 수 있음을 보여준다.

• 한국산학기술학회논문지
• /
• 제11권9호
• /
• pp.3510-3516
• /
• 2010

본 논문은 유아에게 동물의 모습과 습성, 울음소리, 특징 및 한글과 영어 등을 학습하게 하기 위하여 다양한 동물이 있는 동물원을 대상으로 시스템을 개발하였다. 유아가 관심 있는 동물의 전면에 인형을 위치하면, 동물의 울음소리, 모습 등을 동물원 모형에 연결되어 있는 디스플레이 장치를 통하여 학습할 수 있다. 동물원은 현재의 동물원의 모양을 축소하여 각 동물 우리에 동물을 인식할 수 있는 센서를 부착한다. 부착된 센서는 각각 고유의 ID를 가지고 있으며, 유아인형이 접근 하게 되면 인식하고 처리기에 고유 ID를 전송하게 된다. 전송된 ID는 데이터베이스에서 전송된 ID값과 일치하는 콘텐츠를 검색하게 되고, 검색된 콘텐츠는 Output 장치를 통하여 출력되게 된다. 또한 인형이 동물우리에 근접하였을 경우 멀티미디어 효과를 통하여 동물의 울음소리 및 기본적인 학습을 할 수 있도록 하였으며, 한글, 영어, 수셈 학습을 동시에 할 수 있도록 구성하였다.

• Journal of Chest Surgery
• /
• 제28권6호
• /
• pp.533-541
• /
• 1995

We recently developed a new model of moving actuator type totally implantable artificial heart[TIAH , based on the reverse position of the aortic and pulmonary conduits. This concept was proposed by one of surgeons in our team[Joon-Ryang Rho, M.D. to facilitate anatomical fitting of TIAHs. The moving actuator type electromechanical TIAH consisted of the left and right blood sacs, and the moving actuator including a motor. The inverted umbrella type polyurethane valves were used in the blood pumps. The aortic conduit was positioned anterior to the pulmonary conduit, which was the opposite relation to the conventional configuration of other total artificial hearts. We also adapted slip-in connectors for the aortic and pulmonary conduits. Two sheep , weighing 60-69 kg, were used for implantation. After small cervical incision and trans-sternal bilateral thoracotomy, cardiopulmonary bypass [CPB was administered using an American Optical 5-head pump and a membrane oxygenator[Univox-IC, Bentley . The anterior and posterior vena cavae were drained separately for venous return. An arterial return cannula was inserted into the right common carotid artery. During CPB, almost all of the ventricular myocardium was excised down to the atrioventricular groove and the artificial heart was implanted. We achieved 3-day survival in the first sheep and 2-day survival in the second. The day after operation the first sheep was successfully extubated and the second sheep was weaned from a respirator with good condition. After extubation, the first sheep walked around in the cage and fed herself. Serial laboratory and hemodynamic examinations were done during the experiments. In both sheep, pulmonary dysfunction was gradually developed, which was accompanied by acute renal failure. The animals were sacrificed and autopsy was done. Unexpected pregnnacy was incidentally found in both sheep. To our knowledge this is the first report of significant survival cases in the orthotopic implantation of electric TIAH using sheep.

• Journal of Korean Biological Nursing Science
• /
• 제10권2호
• /
• pp.176-183
• /
• 2008

Purpose: The purpose of this study was to identify the effect of Environmental Enrichment (EE) on improvement of motor function in animal models of Parkinson's Disease. Methods: Male C57BL6 mice weighing 25-30 g, at the age of 12 wks were used in this study. The animals were injected MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin, 20 mg/kg in saline, i.p.) 4 times a day at every 2 hr, and raised in EE cage for 14 days. On day 14, after behavior test, all mice were sacrificed for immunohistochemistry. All values were expressed as means$\pm$S.E.M. Statistical significance was evaluated using a one way ANOVA followed by Sheffe test. Results: There was a significant difference between the experimental group and the control group in the behavior test. Also EE significantly reduced of TH positive cell loss in Substantia nigra pars compacta as compared to the result of MPTP treatment. Conclusion: Based on these findings, it is reasonable to assume that the environmental enrichment prevents dopaminergic neuronal loss and improves disarrangement of motor function and behavioral disability induced by MPTP.

• 대한토목학회논문집
• /
• 제31권5D호
• /
• pp.705-717
• /
• 2011

타워크레인은 건설현장의 전체 자재인양 중 약 50%의 물량을 인양하는 양중 장비로 그 수는 빠른 속도로 증가하고 있으나, 건설현장에서의 소홀한 안전관리로 타워크레인 관련 안전사고는 매년 지속적으로 발생되고 있는 실정이다. 특히, 텔레스코핑 작업은 타워크레인 설치단계에서 텔레스코핑 케이지를 유압으로 상승시킨 후 생긴 빈 공간에 새로운 마스트를 삽입하여 요구 높이까지 상승시키는 작업으로 고소에서 다수의 작업자가 동시에 작업을 실시하여 추락 및 낙하의 위험성이 존재하는 등 타 작업에 비해 재해사례 비중이 가장 높은 것으로 분석되었다. 본 연구의 목적은 타워크레인 작업 중 특히 위험성이 높은 것으로 조사 및 분석된 텔레스코핑 작업의 안전성 및 생산성을 향상시킬 수 있는 타워크레인 텔레스코핑 자동화기술의 개념모델을 제안하고 이에 대한 안전성 측면에서의 잠재적 성능을 분석함으로써 향후 타워크레인 텔레스코핑 자동화기술 실물 제작을 위한 원천자료를 제공하는 것이다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제28권6호
• /
• pp.782-787
• /
• 2015

The myogenic regulatory factors is a family of transcription factors that play a key role in the development of skeletal muscle fibers, which are the main factors to affect the meat taste and texture. In the present study, we performed candidate gene analysis to identify single-nucleotide polymorphisms in the MyoD, Myf5, MyoG, and Mrf4 genes using polymerase chain reaction-single strand conformation polymorphism in 360 Erlang Mountain Chickens from three different housing systems (cage, pen, and free-range). The general linear model procedure was used to estimate the statistical significance of association between combined genotypes and muscle fiber traits of chickens. Two polymorphisms (g.39928301T>G and g.11579368C>T) were detected in the Mrf4 and MyoD gene, respectively. The diameters of thigh and pectoralis muscle fibers were higher in birds with the combined genotypes of GG-TT and TTCT (p<0.05). Moreover, the interaction between housing system and combined genotypes has no significant effect on the traits of muscle fiber (p>0.05). Our findings suggest that the combined genotypes of TT-CT and GG-TT might be advantageous for muscle fiber traits, and could be the potential genetic markers for breeding program in Erlang Mountain Chickens.

• Asian-Australasian Journal of Animal Sciences
• /
• 제28권4호
• /
• pp.551-558
• /
• 2015

This study was carried out to evaluate the dietary threonine requirement by measuring the plasma free threonine and ammonia concentrations in rainbow trout, Oncorhynchus mykiss after dorsal aorta cannulation. A total of 70 fish (average initial weight $506{\pm}8.2g$) were randomly distributed into each of the 14 net cages (5 fish/cage). After 48 hours (h) of feed deprivation, each group was intubated at 1% body weight with one of the seven L-amino acid based diets containing graded levels of threonine (0.42%, 0.72%, 0.92%, 1.12%, 1.32%, 1.52%, or 1.82% of diet, dry matter basis). Blood samples were taken at 0, 5, and 24 h after intubation. Post-prandial plasma free threonine concentrations (PPthr) of fish 5 h after intubation with diets containing 1.32% or more threonine were significantly higher than those of fish intubated with diets containing 1.12% or less threonine (p<0.05). Post-absorptive free threonine concentrations (PAthr) after 24 h of intubation of the fish with diets containing 0.92% or more threonine were significantly higher than those of fish intubated with diets containing 0.72% or less threonine. Post-prandial plasma ammonia concentrations (PPA, 5 h after intubation) were not significantly different among fish intubated with diets containing 1.12% or less threonine, except the PPA of fish intubated with diet containing 0.42% threonine. Broken-line model analyses of PPthr, PAthr, and PPA indicated that the dietary threonine requirement of rainbow trout should be between 0.95% (2.71) and 1.07% (3.06) of diet (% of dietary protein on a dry matter basis).

• 한국소음진동공학회논문집
• /
• 제16권2호
• /
• pp.163-168
• /
• 2006

The PWR Nuclear Fuel assembly consists of more than 250 fuel rods that are supported by leaf springs in the cells of more than 10 Spacer Grids (SG) along the rod length. Since it is not easy to conduct mechanical tests on a full-scale model basis, the small-scaled rod bundle $(5\times5)$ which is called partial fuel assembly is generally used for various performance tests during the development stage. As one of the small-scaled tests, a flow test should be carried out in order to verify the performance of the spacer grid to obtain the Flow-Induced Vibration (FIV) characteristics of the scaled fuel assembly over the specified flow range. A vibration test should be also performed to obtain the modal parameters of the assembly prior to the flow test. In this study, we want to develop the estimation procedure of the damping ratio for the scaled test assembly. For the damping factor of the partial fuel assembly and the grid cage at the first vibration mode, as one of the vibration tests, a so-called pluck testing has been performed in air as a preliminary test prior to in-flow damping measurement test. Logarithmic decrement method is used for calculation of the damping ratio. Estimated damping ratio of the partial fuel assembly is about $0.7\%$ with reasonable error of $2\%$ for the previous results. Nonlinear behavior of the partial fuel assembly might be stem mainly from the rod-grid support configuration.

• The Korean Journal of Physiology and Pharmacology
• /
• 제19권3호
• /
• pp.197-202
• /
• 2015

Sulfonylureas are widely used as an antidiabetic drug. In the present study, the effects of sulfonylurea administered supraspinally on immobilization stress-induced blood glucose level were studied in ICR mice. Mice were once enforced into immobilization stress for 30 min and returned to the cage. The blood glucose level was measured 30, 60, and 120 min after immobilization stress initiation. We found that intracerebroventricular (i.c.v.) injection with $30{\mu}g$ of glyburide, glipizide, glimepiride or tolazamide attenuated the increased blood glucose level induced by immobilization stress. Immobilization stress causes an elevation of the blood corticosterone and insulin levels. Sulfonylureas pretreated i.c.v. caused a further elevation of the blood corticosterone level when mice were forced into the stress. In addition, sulfonylureas pretreated i.c.v. alone caused an elevation of the plasma insulin level. Furthermore, immobilization stress-induced insulin level was reduced by i.c.v. pretreated sulfonylureas. Our results suggest that lowering effect of sulfonylureas administered supraspinally against immobilization stress-induced increase of the blood glucose level appears to be primarily mediated via elevation of the plasma insulin level.