• Journal of Plant Biotechnology
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• 제38권4호
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• pp.278-284
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• 2011

The CCR4-CAF1-NOT complex-mediated degradation of mRNA is a fundamental aspect of gene regulation in eukaryotes. We herein examined the role of AtCAF1 in the innate immune and wound responses of plants. Our results showed that overexpression of AtCAF1 significantly downregulated the transcript level of EFR but not FLS2 and BRI1, as well as abolished up-regulated expression pattern of EFR in response to wounding. Consistently, Agrobacteriummediated transient expression of GUS was highly enhanced in the transgenic plants overexpressing AtCAF. Furthermore, JA responsive genes were down-regulated by overexpression of AtCAF, causing the transgenic plants overexpressing AtCAF more susceptible to necrotrophic fungal pathogen, Botrytis cinerea. These results suggest that The CCR4-CAF1-NOT complex-mediated degradation of mRNA negatively regulates wounding-mediated disease resistance in Arabidopsis thaliana.

• BMB Reports
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• 제42권4호
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• pp.227-231
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• 2009

The eukaryotic genome forms a chromatin structure that contains repeating nucleosome structures. Nucleosome packaging is regulated by chromatin remodeling factors such as histone chaperones. The Saccharomyces cerevisiae H3/H4 histone chaperones, CAF-1 and Asf1, regulate DNA replication and chromatin assembly. CAF-1 function is largely restricted to non-transcriptional processes in heterochromatin, whereas Asf1 regulates transcription together with another H3/H4 chaperone, HIR. This study examined the role of the yeast H3/H4 histone chaperones, Asf1, HIR, and CAF-1 in chromatin dynamics during transcription. Unexpectedly, CAF-1 was recruited to the actively transcribed region in a similar way to HIR and Asf1. In addition, the three histone chaperones genetically interacted with Set2-dependent H3 K36 methylation. Similar to histone chaperones, Set2 was required for tolerance to excess histone H3 but not to excess H2A, suggesting that CAF-1, Asf1, HIR, and Set2 function in a related pathway and target chromatin during transcription.

• 한국환경보건학회지
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• 제42권1호
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• pp.19-26
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• 2016

Objectives: This study aimed to assess temporal changes in the level of culturable airborne fungi (CAF) in three microbiology laboratories and determine the environmental factors associated with CAF level. Methods: CAF levels were determined once per month from March 2011 to February 2012 in three microbiology laboratories. An Andersen one-stage sampler was used for five minutes, three times per day to collect the CAF. Arithmetic means of CAF concentrations and standard deviation (SD) were calculated. A Mann-Whitney test was applied to compare the differences between environmental factors such as divided room by structure of laboratory, use of humidifier, and use of air-conditioner. Correlation analysis was also applied to identify the association between CAF concentrations and environmental factors. Results: CAF levels demonstrated an increasing tendency in summer, and the three laboratories showed consistent seasonal patterns. Temperature and relative humidity (RH) were associated with CAF levels. When the humidifier was off, CAF concentrations were significantly higher in study rooms than in study rooms in which the humidifier was on. Conclusion: CAF levels in indoor microbiology laboratories varied greatly depending upon the temperature and RH and whether a humidifier was used.

• Architectural research
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• 제8권1호
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• pp.9-16
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• 2006

Maximizing profit is the most significant object of commercial places. Especially for some places where its atmosphere is main concern of customers like $Caf{\acute{e}}s$, interior design is crucial. Thus it needs to evaluate human emotion and preference. Human Sensibility Ergonomics which is recently signified in various fields is an attempt to quantitatively analyze factors of human sensibility and apply it to design operation. This study attempted to infuse human sensibility ergonomics into $Caf{\acute{e}}$ interior design and analyzed preferable $Caf{\acute{e}}$ interior design factors. Because most $Caf{\acute{e}}s$ have their own target customers, so we assumed four different target customer groups divided by their age and gender groups in each age group. As a result, the difference of each group could be examined. Middle-aged group and males have main interest in the intensity of luminance, i.e. the brightness, whereas youths and females attach importance to both quality of light and color. In addition, there is a tendency for middle-aged group and males to see $Caf{\acute{e}}$ as a public place while young people and females recognize $Caf{\acute{e}}$ as a place for entertainment. Taking the result of this study into consideration can lead to successful $Caf{\acute{e}}$ interior design for specific target customers.

• 감성과학
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• 제14권4호
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• pp.663-674
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• 2011

점선의 추가와 주변 자극의 추가가 caf$\grave{e}$ wall 착시에서 지각된 착시량과 착시에 대한 확신도에 미치는 효과를 알아보기 위해 두 편의 실험을 수행하였다. 실험 1에서는 흰 색과 검은 색 벽돌이 나열된 세 줄의 벽돌층의 수평 경계에 흰색 벽돌과 검은색 벽돌의 중간 밝기를 가지는 회색 선분(모르타르:mortar)과 벽돌과 반대 밝기의 점선을 추가하였을 때 착시에 미치는 효과를 알아보았다. 실험 2에서는 실험 1에서 사용한 자극에 벽돌 층을 추가하여 주변자극의 추가에 따라 caf$\grave{e}$ wall 착시가 어떻게 달라지는지 살펴보았다. 실험 결과 회색 경계선에 점선을 추가하는 것은 착시 지각의 확신도에는 가산적으로 영향을 미치는 것으로 나타났으나 지각된 착시량에서는 가산적 영향이 나타나지 않았다. 반면 벽돌 층의 추가는 지각된 착시량에는 영향을 미쳤지만 착시지각의 확신도에는 영향을 미치지 않는 것으로 나타났다. 논의에서는 이러한 결과가 시각피질의 방위 선별적 세포의 반응과 관련이 있을 가능성에 대해 제안하였다.

• Journal of Oral Medicine and Pain
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• 제44권1호
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• pp.35-39
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• 2019

Calcifying aponeurotic fibroma (CAF) is an uncommon benign soft-tissue fibroblastic tumor with characteristic histological features. It mainly occurs in the distal part of the extremities, such as the hands and feet, in children and adolescents. Males are twice as commonly affected as females. CAF exhibits local invasiveness, and hence, its recurrence rate is also high. Therefore, complete surgical excision is both diagnostic and therapeutic. The occurrence of CAF in the maxillofacial region, especially the temporomandibular joint (TMJ), is very rare, and this necessitates its differentiation from other TMJ neoplasms. The differential diagnosis of CAF requires microscopic examination. Herein, we report a rare case of CAF located at the left mandibular condyle, which was confirmed by histopathological analysis.

• 한국위성정보통신학회논문지
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• 제4권1호
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• pp.21-27
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• 2009

본 논문에서는 임의의 전파를 발사하는 송신원의 위치를 파악하기 위한 방법의 하나로써 시간 차이와 주파수 차이를 이용하는 교차모호함수를 사용하여 시뮬레이션 한 결과를 소개한다. 동일한 송신 신호가 서로 다른 경로를 통하여 수신될 경우 두 신호가 가지고 있는 시간 지연의 차이가 있고, 또 서로 다른 도플러 천이로 인하여 주파수 천이를 가지게 된다, 이러한 성질을 이용하여 서로 다른 시간 지연과 주파수 천이를 가지는 두 신호에 대한 교차모호함수를 계산하고, 이 함수가 최대 값을 가지도록 하는 시간 및 주파수 천이를 구하여 전파를 발사한 송신국의 위치를 파악할 수 있게 된다. 본 논문에서는 이러한 교차모호 함수를 이용하여 여러 가지 환경에 따른 시뮬레이션 결과를 소개하고, CAF 계산의 효율성을 개선하고 성능을 향상시키는 방법에 대해 소개한다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권10호
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• pp.4245-4250
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• 2014

Emerging evidence has suggested that glycolysis is enhanced in cancer-associated fibroblasts (CAF), and miR-186 is downregulated during the CAF formation. However, it is not clear whether miR-186 is involved in the regulation of glycolysis and what the role of miR-186 plays during the CAF formation. In this study, quantitative PCR analysises show miR-186 is downregulated during the CAF formation. Moreover, miR-186 targets the 3' UTR of Glut1, and its overexpression results in the degradation of Glut1 mRNA, which eventually reduces the level of Glut1 protein. On the other hand, knockdown of miR-186 increased the expression of Glut1. Both time course and dose response experiments also demonstrated that the protein and mRNA levels of Glut1 increase during CAF formation, according to Western blot and quantitative PCR analyses, respectively. Most importantly, besides the regulation on cell cycle progression, miR-186 regulates glucose uptake and lactate production which is mediated by Glut1. These observations suggest that miR-186 plays important roles in glycolysis regulation as well as cell cycle checkpoint activation.

• 한국방재학회 논문집
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• 제10권5호
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• pp.35-40
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• 2010

본 연구에서는 RC슬래브 교량의 내하력을 향상시키기 위해 유리섬유 복합재(CAF)를 이용한 보강방법을 제시하였다. 보강 성능을 평가하기 위해 설계하중 DB 18로 설계된 실험대상 교량의 슬래브 하면에 단위폭당 50 cm로 CAF 복합재를 보강하여 정 동적재하시험을 수행하였다. 정 동적재하시험 결과 RC슬래브의 최대변위, 변형률, 충격계수는 보강전에 비하여 보강후에 각 14.7%, 5.0% 및 33.7% 감소하였다. 시험경간 중앙부에서의 RC슬래브의 공칭저항비는 27% 증가하였고, 공용내하력은 1,2 경간에서 44.6%, 48.9%증가하였다. 결론적으로 설계하중 DB 18로 설계된 노후된 RC슬래브 교량을 현행 1등급교의 설계하중 DB 24로 향상시키는데 CAF 복합재를 이용한 보강방법은 매우 효과적인 것으로 판단된다.

• Advances in Traditional Medicine
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• 제8권4호
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• pp.416-422
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• 2008

This study evaluated the anti-inflammatory potential of the crude alkaloidal fraction (CAF) of methanol extract of Solanum trilobatum Linn. (Solanacea) root in animal models of inflammation. Crude alkaloidal fraction at doses of 25, 50 and 100 mg/kg significantly (p < 0.01) reduced carrageenan induced rat paw volume at 3 h after carrageenan challenge as compared to control group of animals. CAF (25, 50 and 100 mg/kg) significantly (p < 0.01) and dose dependently suppressed cotton pellet induced granuloma formation. Topical application of CAF (1, 5 and 10 mg/ear) markedly inhibited multiple application of TPA in mice. CAF elicited pronounced inhibitory effects on formaldehyde and adjuvant induced arthritis in rats. These results indicate that CAF of methanol extract of the Solanum trilobatum has anti-inflammatory activity in acute and chronic inflammation.