• Journal of Plant Biotechnology
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• v.38 no.4
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• pp.278-284
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• 2011

The CCR4-CAF1-NOT complex-mediated degradation of mRNA is a fundamental aspect of gene regulation in eukaryotes. We herein examined the role of AtCAF1 in the innate immune and wound responses of plants. Our results showed that overexpression of AtCAF1 significantly downregulated the transcript level of EFR but not FLS2 and BRI1, as well as abolished up-regulated expression pattern of EFR in response to wounding. Consistently, Agrobacteriummediated transient expression of GUS was highly enhanced in the transgenic plants overexpressing AtCAF. Furthermore, JA responsive genes were down-regulated by overexpression of AtCAF, causing the transgenic plants overexpressing AtCAF more susceptible to necrotrophic fungal pathogen, Botrytis cinerea. These results suggest that The CCR4-CAF1-NOT complex-mediated degradation of mRNA negatively regulates wounding-mediated disease resistance in Arabidopsis thaliana.

• BMB Reports
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• v.42 no.4
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• pp.227-231
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• 2009

The eukaryotic genome forms a chromatin structure that contains repeating nucleosome structures. Nucleosome packaging is regulated by chromatin remodeling factors such as histone chaperones. The Saccharomyces cerevisiae H3/H4 histone chaperones, CAF-1 and Asf1, regulate DNA replication and chromatin assembly. CAF-1 function is largely restricted to non-transcriptional processes in heterochromatin, whereas Asf1 regulates transcription together with another H3/H4 chaperone, HIR. This study examined the role of the yeast H3/H4 histone chaperones, Asf1, HIR, and CAF-1 in chromatin dynamics during transcription. Unexpectedly, CAF-1 was recruited to the actively transcribed region in a similar way to HIR and Asf1. In addition, the three histone chaperones genetically interacted with Set2-dependent H3 K36 methylation. Similar to histone chaperones, Set2 was required for tolerance to excess histone H3 but not to excess H2A, suggesting that CAF-1, Asf1, HIR, and Set2 function in a related pathway and target chromatin during transcription.

• Journal of Environmental Health Sciences
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• v.42 no.1
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• pp.19-26
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• 2016

Objectives: This study aimed to assess temporal changes in the level of culturable airborne fungi (CAF) in three microbiology laboratories and determine the environmental factors associated with CAF level. Methods: CAF levels were determined once per month from March 2011 to February 2012 in three microbiology laboratories. An Andersen one-stage sampler was used for five minutes, three times per day to collect the CAF. Arithmetic means of CAF concentrations and standard deviation (SD) were calculated. A Mann-Whitney test was applied to compare the differences between environmental factors such as divided room by structure of laboratory, use of humidifier, and use of air-conditioner. Correlation analysis was also applied to identify the association between CAF concentrations and environmental factors. Results: CAF levels demonstrated an increasing tendency in summer, and the three laboratories showed consistent seasonal patterns. Temperature and relative humidity (RH) were associated with CAF levels. When the humidifier was off, CAF concentrations were significantly higher in study rooms than in study rooms in which the humidifier was on. Conclusion: CAF levels in indoor microbiology laboratories varied greatly depending upon the temperature and RH and whether a humidifier was used.

• Architectural research
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• v.8 no.1
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• pp.9-16
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• 2006

Maximizing profit is the most significant object of commercial places. Especially for some places where its atmosphere is main concern of customers like $Caf{\acute{e}}s$, interior design is crucial. Thus it needs to evaluate human emotion and preference. Human Sensibility Ergonomics which is recently signified in various fields is an attempt to quantitatively analyze factors of human sensibility and apply it to design operation. This study attempted to infuse human sensibility ergonomics into $Caf{\acute{e}}$ interior design and analyzed preferable $Caf{\acute{e}}$ interior design factors. Because most $Caf{\acute{e}}s$ have their own target customers, so we assumed four different target customer groups divided by their age and gender groups in each age group. As a result, the difference of each group could be examined. Middle-aged group and males have main interest in the intensity of luminance, i.e. the brightness, whereas youths and females attach importance to both quality of light and color. In addition, there is a tendency for middle-aged group and males to see $Caf{\acute{e}}$ as a public place while young people and females recognize $Caf{\acute{e}}$ as a place for entertainment. Taking the result of this study into consideration can lead to successful $Caf{\acute{e}}$ interior design for specific target customers.

• Science of Emotion and Sensibility
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• v.14 no.4
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• pp.663-674
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• 2011

Two experiments were conducted to examine how additional dotted lines and peripheral stimulus to the caf$\grave{e}$ wall illusion affect on the degree of perceived tilt (amount of illusion) and confidency of illusionary perception. The experiment 1 tested the effects of adding mortar (solid line) and bright contrast dotted lines to the borderline of 3 layers of blocks, which are consisted of black and white blocks. On the experiment 2, effects of adding peripheral stimulus on caf$\grave{e}$ wall illusion were tested. The results of experiment 1 showed that adding dotted line on the borderline mortar between blocks has additive effects on confidency of illusory perception. However, no effect on perceived amount of illusion was noted. On the other hand according to the results of experiment 2, adding blocks did increase amount, but not confidency of illusory perception. These results imply that the perception of caf$\grave{e}$ wall illusion is related with response of orientation selective neurons.

• Journal of Oral Medicine and Pain
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• v.44 no.1
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• pp.35-39
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• 2019

Calcifying aponeurotic fibroma (CAF) is an uncommon benign soft-tissue fibroblastic tumor with characteristic histological features. It mainly occurs in the distal part of the extremities, such as the hands and feet, in children and adolescents. Males are twice as commonly affected as females. CAF exhibits local invasiveness, and hence, its recurrence rate is also high. Therefore, complete surgical excision is both diagnostic and therapeutic. The occurrence of CAF in the maxillofacial region, especially the temporomandibular joint (TMJ), is very rare, and this necessitates its differentiation from other TMJ neoplasms. The differential diagnosis of CAF requires microscopic examination. Herein, we report a rare case of CAF located at the left mandibular condyle, which was confirmed by histopathological analysis.

• Journal of Satellite, Information and Communications
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• v.4 no.1
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• pp.21-27
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• 2009

In this paper, we introduce a simulation result of cross ambiguity function (CAF) using time difference of arrival and frequency difference of arrival. This method is generally used to find a geographical location of the unknown radio transmitter. If multiple signals with the same source information are arrived at a receiver via different paths, then they will have different time delays and Doppler shifts. We can estimate the CAF by using these characteristics, and estimate the location of the unknown transmitter. This paper introduces a technique to improve the estimation performance of CAF.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.10
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• pp.4245-4250
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• 2014

Emerging evidence has suggested that glycolysis is enhanced in cancer-associated fibroblasts (CAF), and miR-186 is downregulated during the CAF formation. However, it is not clear whether miR-186 is involved in the regulation of glycolysis and what the role of miR-186 plays during the CAF formation. In this study, quantitative PCR analysises show miR-186 is downregulated during the CAF formation. Moreover, miR-186 targets the 3' UTR of Glut1, and its overexpression results in the degradation of Glut1 mRNA, which eventually reduces the level of Glut1 protein. On the other hand, knockdown of miR-186 increased the expression of Glut1. Both time course and dose response experiments also demonstrated that the protein and mRNA levels of Glut1 increase during CAF formation, according to Western blot and quantitative PCR analyses, respectively. Most importantly, besides the regulation on cell cycle progression, miR-186 regulates glucose uptake and lactate production which is mediated by Glut1. These observations suggest that miR-186 plays important roles in glycolysis regulation as well as cell cycle checkpoint activation.

• Journal of the Korean Society of Hazard Mitigation
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• v.10 no.5
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• pp.35-40
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• 2010

The present study proposes the strengthening method to use Conclinic Advanced FiberWrep(CAF) so as to improve Load Carrying Capacity of the RC slab bridge. In order to evaluate the strengthening performance, we strengthen 50cm per unit-width of CAF to the slab's bottom of the test bridge that designed with DB 18, then perform Static and Dynamic Field Load Test. As a result of this, 14.7% of the maximum displacement, 5.0% of the strain and 33.7% of the impact factor are reduced after strengthening. At the middle of the test spans, nominal resisting ratio is increased by 27% and Service Load Carrying Capacity is increased by 44.6%, 48.9% of each span 1 and 2. In conclusion, this study indicates that the strengthening method using CAF is very effective to improve the deteriorated RC slab bridge designed with DB 18, to the DB 24 of the first class bridge design load.

• Advances in Traditional Medicine
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• v.8 no.4
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• pp.416-422
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• 2008

This study evaluated the anti-inflammatory potential of the crude alkaloidal fraction (CAF) of methanol extract of Solanum trilobatum Linn. (Solanacea) root in animal models of inflammation. Crude alkaloidal fraction at doses of 25, 50 and 100 mg/kg significantly (p < 0.01) reduced carrageenan induced rat paw volume at 3 h after carrageenan challenge as compared to control group of animals. CAF (25, 50 and 100 mg/kg) significantly (p < 0.01) and dose dependently suppressed cotton pellet induced granuloma formation. Topical application of CAF (1, 5 and 10 mg/ear) markedly inhibited multiple application of TPA in mice. CAF elicited pronounced inhibitory effects on formaldehyde and adjuvant induced arthritis in rats. These results indicate that CAF of methanol extract of the Solanum trilobatum has anti-inflammatory activity in acute and chronic inflammation.