• Title/Summary/Keyword: CAD

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Characterization of cadC and cadR Mutants in Mediating the Expression of the Salmonella typhimurium cadBA Operon (Salmonella typhimurium cadBA 오페론의 발현에 관여하는 돌연변이체의 선별 및 그 특성)

  • 방성호;박용근
    • Korean Journal of Microbiology
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    • v.37 no.4
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    • pp.259-264
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    • 2001
  • It has been well known that the expression of S. typhimurium cadBA operon requires at least two extracellular signals: low pH and high concentration of lysine. To better understand the nature of pH-dependent and lysine dependent signal transduction, mutants were isolated in JF2238(cadA-lacZ) by Tn10 insertion, spontaneous mutagenesis, and EMS treatment. Two mutants were isolated from JF2238, expressed as a cadA-lacZ operon fusion in various growth conditions, and analyzed to have mutations in cadC, a gene encoding a function necessary for transcriptional activation of cadBA. One isolate (cadC6) conferred pH-independent and lysine-independent cadBA expression and the other(cadC4) showed pH-independent and lysine-dependent cadBA expression. cadR::Tn10 and cadR4 mutants were expressed in the absence of exogenously added lysine. They were also resistant to thiosine and complemented by lysP clone from E. coli. Thus, in the absence of exogenous lysine, cadR is a negative regulator of cadBA expression. Cadaverine, the product of lysine decarboxylation, was shown to inhibit expression of cadA-lacZ fusion in cad $C^+$ cell.

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Activation of the Vibrio vulnificus cadBA Operon by Leucine-Responsive Regulatory Protein is Mediated by CadC

  • Rhee, Jee-Eun;Kim, Kun-Soo;Choi, Sang Ho
    • Journal of Microbiology and Biotechnology
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    • v.18 no.11
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    • pp.1755-1761
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    • 2008
  • The present study revealed that Lrp, a leucine-responsive regulatory protein, is involved in the regulation of cadBA transcription through activation of $P_{cadBA}$. The influence of Lrp on $P_{cadBA}$ was mediated by CadC, and thereby, CadC was able to compensate for the lack of Lrp in the activation of $P_{cadBA}$. Western blot analyses and EMSA demonstrated that the cellular level of CadC was not significantly affected by Lrp, and that Lrp exerted its effect by directly binding to $P_{cadBA}$. These combined results suggested that CadC and Lrp function cooperatively to activate the $P_{cadBA}$ rather than sequentially in a regulatory cascade.

Cloning and Sequencing Analysis of cadC Encoding Transcriptional Activator CadC from Salmonella typhimurium

  • Kim, Bae-Hoon;Lee, Ho-Jeong;Lee, In-Soo;Bang, Sung-Ho;Kim, Joon;Park, Yong-Keun
    • Journal of Microbiology
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    • v.39 no.2
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    • pp.109-115
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    • 2001
  • Salmonella typhimurium possesses a cad operon, which contributes to an adaptive response against an acidifying environment. In Escherichia coli, the activation of the cad operon is dependent on cadC, which is located upstream of the operon. However, the activator of cad operon in S. typhimurium has not been known until now. In this study, we selected a putative cadC mutant by trasposon mutagenesis and cloned the cadc of S. typhimurium. Moreover, the cadC mutant was complemented by cadC clone. The cadC gene from S. typhimurium LT-2 consists of 1539 bp encoding a polypeptide ob 512 amino acids, and shows sequence similarity to cadC of E. coli with 53% identity and 67% similarity. The hydrophobicity profile of th S. typhimurim CadC sequence is very similar to E. coli CadC.

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The Present Situations of the Apparel CAD Education in Clothing Departments in Domestic Colleges (국내 의류학분야 학과의 어패럴CAD 교육현황)

  • Kim, Su-Hyun;Lee, Song-Ja
    • Fashion & Textile Research Journal
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    • v.1 no.1
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    • pp.26-33
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    • 1999
  • Nowadays the Apparel CAD is required in the clothing industries. So many domestic Apparel education institutions are interested in the Apparel CAD education. By investigating the present Apparel CAD education situations in college, the writer shows some data that are helpful to solve the problems in these parts. The purpose of this study is to give some helps in teaching the Apparel CAD in college. The results of this study are as followings, as the use of CAD systems is growing in the clothing industries, many colleges are concerned with the Apparel CAD. As a result, many colleges equip the Apparel CAD systems and the Apparel CAD is taught as special lectures, the part of other courses or regular course in many colleges. Apparel CAD should be taught as a regular course in colleges. And it should be taught systematically with Design CAD and Pattern CAD. But it is taught limitedly as special lectures, the part of other courses and post graduate studies. So it is impossible to educate the students professionally. As many colleges the CAD equipments are not opened to the students, the student's use of CAD systems are limited, so educational environment needs to improve to use CAD systems freely.

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A Study on Environment and Perception of CAD by Undergraduate Students in the Dept. of Architecture - Case study on Undergraduate Students - (대학(大學) 건축학과(建築學科) 재학생(在學生)의 CAD 환경(環境) 및 인식(認識)에 관한 연구(硏究) - 사례대학의 재학생을 대상으로 -)

  • Yoo, Chang-Geun;Park, Sung-Ha
    • Journal of The Korean Digital Architecture Interior Association
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    • v.1 no.1
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    • pp.24-30
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    • 2001
  • This study conducts a questionnaire with undergraduate students in the Dept. of Architecture who will lead the architectural field in future, examines environment and perception of CAD in their home and universities and aims at supplying the data required for setting CAD educational index and building its use environment, and it could obtain the following results. Individual CAD environment of undergraduate students in the Dept. of Architecture reach a considerable level in hardware part, but they don't have the same level in software part as the hardware and use illegal copy programs. It is shown that they spend their time in using CAD for five to eight hours a week and its main purposes are to perform a project related to architectural design or make report requiring drawing. Major places using CAD are CAD room in university or public PC room equipped with CAD compared to their own houses and most of them have a negative recognition of the convenience of its use. Their satisfaction with CAD use is considerably high and when they submitted their assignments of architectural design project by means of CAD, they had a positive evaluation from their professors and they have such hopes that 'Korean Support Strengthening', 'lowering price through version supply for students' and 'diversification of design symbol' in CAD S/W, and especially, most of respondents have an intention to purchase the original goods when version only for students will be marketed in future. Accordingly, for a qualitative improvement of CAD environment for undergraduate students in the Dept. of Architecture, universities must be equipped with the various types of CAD S/W and Applications and students' opportunity to access them should be increased. In addition, a method which can enhance using convenience of CAD room and PC room is required and CAD related S/W developers must market the version for students which consider the reality of undergraduates in Korea properly with an appropriate price level in order to settle a sound S/W culture.

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Development of Integrated CAD framework for ASIC Design (ASIC 설계용 통합 CAD Framework 개발)

  • 엄성용;신혜선;이규원;박선화
    • Journal of Internet Computing and Services
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    • v.2 no.4
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    • pp.25-32
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    • 2001
  • The CAD tools for ASIC design, which are already developed or will be developed in the future, have their own functions and different working environments in many cases. Therefore, it would be more effective in achieving the final design goal, if we have a system called CAD framework in which these CAD tools are systematically integrated. In this paper, we introduce same novel techniques for integrating systematically such the CAD tools, which are usually developed under UNIX shell environments, into the CAD framework with the standard graphics interface such as X-windows. Some meta languages and script file formats are developed for flexible specification of the system MENU hierarchy and the data dependencies among executable programs. We integrated two existing CAD tools into our CAD framework using the techniques and find out the integrated protype system is working well under the new system environments.

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Windows 시대의 퍼스널 CAD

  • 광성풍명;김석일
    • CDE review
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    • v.2 no.1
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    • pp.1-6
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    • 1996
  • CG 등의 컴퓨터 지원에 의한 도형처리 시스템 중에서 특히 설계제도를 지원하는 시스템으로서 CAD가 제공되고 있다. CAD에는 대형 컴퓨터를 플랫폼으로 하는 메인프레임(집중형) CAD와 EWS(엔지니어링 워크 스테이션)을 플랫폼으로 하는 EWS/CAD 및 퍼스널 컴퓨터를 베이스로 한 퍼스널 CAD가 있는데 최근에 컴퓨터의 고성능화와 소형화의 흐름에 따라 퍼스널 CAD가 CAD의 주류로 되고 있다. 그 큰 요인으로서 퍼스널 CAD는 하드웨어 및 소프트웨어를 포함한 시스템 가격이 EWS/CAD 등을 비롯한 다른 CAD에 비하여 저렴한 가격대라는 것과 조작성이 우수하다는 점을 들 수 있다. 또한 최근에는 OS(Operating System: 컴퓨터를 움직이기 위한 기본적 소프트웨어)의 주류가 싱글 유저.싱글 태스크인 MS-DOS(이하 DOS라 한다)에서 싱글 유저.의사 멀티 태스크인 MS-Windows(이하 Windows라 한다)에로 이행하고 있으며 퍼스널 CAD도 그에 대응하는 것으로 되고 있다. 여기서는 이 Windows에 대응하는 퍼스널 CAD의 최신의 기술동향 및 OS에 대하여 설명한다.

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Design of Lightweight CAD Files with Dimensional Verification Capability for Web-Based Collaboration (웹기반 협업을 위한 치수검증이 가능한 경량캐드파일 설계)

  • Song In-Ho;Chung Sung-Chong
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.30 no.5 s.248
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    • pp.488-495
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    • 2006
  • The demand for the use of 3D CAD data over the Internet environment has been increased. However, transmission of 3D CAD data has delayed the communication effectiveness because of the CAD data size. Lightweight CAD file design methodology is required for rapid transmission in the distributed environment. In this paper, to derive lightweight CAD files from commercial CAD systems, a file translation system producing a native file is constructed first by using the InterOp and API of the ACIS kernel. Using the B-rep model and mesh data extracted from the native file, the lightweight CAD file with topological information is constructed as a binary file. Since the lightweight CAD file retains topological information, it is applied to the dimensional verification, digital mock-ups and visualization of CAD files. Effectiveness of the proposed lightweight CAD file is confirmed through various case studies.

A Study on the Characteristics of Drafting Process by Using CAD (CAD를 이용한 도면 작성의 과정적 특성에 관한 연구 -AutoCAD 교재에 나타난 설계 사례를 중심으로-)

  • 홍승대
    • Korean Institute of Interior Design Journal
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    • no.18
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    • pp.16-22
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    • 1999
  • The purpose of this study is to analyze the drafting process by using AutoCAD for the basic course as well as to suggest the educational basic data of CAD in interior desigv. This research is to show not only practical use of CAD as a drafting tool function, but offering optimizing ways of AutoCAD command usage. The method used for this study is based on an analyzing of 6 types of total 98 types AutoCAD tutorial manual in CAD section of Kyobo book center. After the analysis of the AutoCAD command usage in creating floor plan, characteristics of drafting process is nearly the same as traditional drafting process. It means that traditional drafting process grafted on to computer aided drafting process. And the practical usage of AutoCAD command type is Draw, Modify, Format and View command in creating floor plav. That is emphasis on a specific functiov. Therefore, it should be developed basic course program which adapted various function of AutoCAD.

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CAD/CAPP System based on Manufacturing Feature Recognition (제조특징인식에 의한 CAD/CAPP 시스템)

  • Cho, Kyu-Kab;Kim, Suk-Jae
    • Journal of the Korean Society for Precision Engineering
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    • v.8 no.1
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    • pp.105-115
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    • 1991
  • This paper describes an integrated CAD and CAPP system for prismatic parts of injection mold which generates a complete process plan automatically from CAD data of a part without human intervention. This system employs Auto CAD as a CAD model and GS-CAPP as an automatic process planning system for injection mold. The proposed CAD/CAPP system consists of three modules such as CAD data conversion module, manufacturing feature recognition module, and CAD/CAPP interface module. CAD data conversion module transforms design data of AutoCAD into three dimensional part data. Manufacturing feature recognition module extracts specific manufacturing features of a part using feature recognition rule base. Each feature can be recognized by combining geometry, position and size of the feature. CAD/CAPP interface module links manufacturing feature codes and other head data to automatic process planning system. The CAD/CAPP system can improve the efficiency of process planning activities and reduce the time required for process planning. This system can provide a basis for the development of part feature based design by analyzing manufacturing features.

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