• Plant Resources
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• 제8권2호
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• pp.110-115
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• 2005

Ribosomal protein complex with ribosomal RNA to form the subunits of the ribosome serve essential functions in protein synthesis. A full-length cDNA (PRPS4) encoding ribosomal protein S4 has been isolated and its nucleotide sequence determined in ginseng plant (Panax ginseng). A PRPS4 cDNA is 1105 nucleotides long and has an open reading frame of 792 bp with a deduced amino acid sequence of 264 residues (pI 10.67). The deduced amino acid sequence of PRPS4 matched to the previously reported ribosomal protein S4 genes. Their degree of amino acid identity ranged from 68 to $92\%$. Phylogenetic analysis based on the amino acid residues showed that the PRPS4 grouped with ribosomal protein S4 of S. tuberosum (CAA54095).

• Plant Resources
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• 제7권3호
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• pp.174-180
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• 2004

Calmodulin, a $Ca^{2+}$-binding protein, has no enzyme activity. It combines with $Ca^{2+}$ and makes variable proteins to an active form. Calmodulin 2 is a ubiquitous protein in plants. To investigate the defense mechanism against various stresses, a clone encoding a calmodulin 2 protein was isolated from a cDNA library prepared from taproot mRNAs of Codonopsis lanceolata. The cDNA, designated CICAM2, is 719 nucleotides long and has an open reading frame of 450 bp with a deduced amino acid sequence of 149 residues. The deduced amino acid sequence of CICAM2 showed a high similarity with calmodulins of P. x hybrida (P27163) 97%, N. tabacum (BAB61908) 97%, S. tuberosum (AAA74405) 96%, Z. mays (CAA74307) 92%, C. richardii (AF510075) 93%, M. truncatula (AAM81203) 91%, and G. max (P62163) 91%. The transcriptional expression of the CICAM2 gene, was gradually increased by the CaCl$_2$ treatment. Whereas its expression And it was gradually decreased in the cold stress treatment.ent.

• 한국중재학회지:중재연구
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• 제26권3호
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• pp.121-147
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• 2016

Factual issues in most patent litigation are related to very complicated techniques. Thus, the courts has emphasised that the technology in dispute has to be read and understood through the eyes of a person to whom it is directed. Therefore, among the various processes in federal litigation, most litigation in the field of patent infringement relies on at least some expert evidence. This paper focuses on issues regarding patent dispute evidence, and explore whether there are unresolved issues in evidential rules and procedures of patent proceedings. Further, this paper seeks to demonstrate that both the parties and the courts in patent disputes generally benefit from the current evidence system. However, in a number of Australian cases, the scope of expert evidence in patent cases has been strictly limited. Australian Government identified uncertain issues associated with the present patent enforcement system, due to factors such as a low level of knowledge about what patent rights entail, the high degree of uncertainty of outcome in legal proceedings, etc. Arbitration shall be reviewed and suggested as an alternative to tackling the ongoing problems in the trial system.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2013년도 추계학술대회 논문집
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• pp.775-780
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• 2013

In recent years, a notebook like an ultrabook gets thinner. Its thickness causes problems in cooling fan performance, system installation condition, and so on. In this study, we installed a small turbofan in notebook system with very narrow gap in order to generate similar condition to a real product. Experiments were performed to measure the fan's performance and the flow and noise characteristics, its results were compared with computational ones. Prediction of P-Q curve using CFD showed under about 5% error in high flow rate and its trend was agreed with experimental one over the flow field. Experimental data to measure the noise at a distance of 100 mm from a rotation axis direction of an impeller corresponded well with computational ones of broadband and BPF noise. The noise experiments to measure at a distance of 100 mm from a rotation axis direction of an impeller corresponded well with computational ones of broadband and BPF noise. Especially, tip part of impeller blade and part of exit and bottom near in an analysis by a commercial program(FlowNoise).

• 한국약용작물학회지
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• 제17권3호
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• pp.198-203
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• 2009

Isatis tinctoria var. yezoensis (Ohwi) Ohwi (Cruciferae) is one of major natural dyeing crops in the world and also have used as a medicinal plant in Korea. We evaluated seed purity in $F_1$-hybrid accessions using amplified fragment length polymorphism (AFLP) markers. One hundred sixty seeds from the male and female harvests were subsequently screened for seed purity with ten primers. The 13 accession-specific bands and many variable AFLP bands scored for accessions. Especially, E-AAC/M-CAA and E-AAG/M-CAT were presented clear hybrid bands for $F_1$ hybrids. $F_1$ hybrids maintained higher average level of genetic diversity compared with their correspondent parents. Self-inbred seeds from the female and male harvests were revealed 8.0% and 5.0%, respectively. The AFLP may lead to a better insight in to the hybrid seed purity test in I. tinctoria var. yezoensis.

• Journal of the Optical Society of Korea
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• 제17권6호
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• pp.525-532
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• 2013

Contrary to the academic interests of other existing studies elsewhere, this study deals with how the alignment algorithms such as sensitivity or Differential Wavefront Sampling (DWS) can be better used under effects from field, compensator positioning and environmental errors unavoidable from the shop-floor alignment work. First, the influences of aforementioned errors to the alignment state estimation was investigated with the algorithms. The environmental error was then found to be the dominant factor influencing the alignment state prediction accuracy. Having understood such relationship between the distorted system wavefront caused by the error sources and the alignment state prediction, we used it for simulated and experimental alignment runs for Infrared Optical System (IROS). The difference between trial alignment runs and experiment was quite close, independent of alignment methods; 6 nm rms for sensitivity method and 13 nm rms for DWS. This demonstrates the practical usefulness and importance of the prior error analysis using the alignment algorithms before the actual alignment runs begin. The error analysis methodology, its application to the actual alignment of IROS and their results are described together with their implications.

• 한국균학회지
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• 제23권3호통권74호
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• pp.202-208
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• 1995

본 실험결과 느타리버섯속에서의 재현성 있는 최적 RAPD 조건은 $50\;{\mu}l$ 반응액에서 80 ng template DNA, 30 pmole primer, $200\;{\mu}M$ dNTP, 2 mM $MgCl_2$, 50 mM KCl, 10 mM Tris-HCI(pH 9.0), 0.1% Triton X-100, 1.5 unit Taq polymerase(promega)였다. 여섯 개의 primer가 Pleurotus속 8종의 균주에서 RAPD polymorphism을 보임을 알 수 있었으며, 이들의 염기배열은 PR2(GGG GGG AAG C), PR3(GCG GTT GAG G), PR4(CGC ACC GCA C), PR10(CAA TCG CCG T), PR11(CAG CAC CCA C), PR17(TAG GCG TAT CAG GAG GCC CT)이었다.

• 대한기계학회논문집A
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• 제38권3호
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• pp.269-274
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• 2014

본 논문에서는 사보니우스형 풍력터빈의 저소음 설계에 관한 연구를 수행하였다. 선행연구를 통해 BPF 보다 높은 주파수를 기본주파수로 가지며 발생하는 하모닉 성분의 순음소음이 사보니우스형 풍력터빈의 주요한 소음임을 밝혔고, 이러한 하모닉 성분의 소음은 와류에 의한 것임을 확인하였다. 본 연구에서는 이러한 선행연구결과를 바탕으로, 사보니우스형 풍력터빈의 저소음 설계를 위해 이탈되는 와류에 위상차를 유도할 수 있는 터빈 날개 끝단을 도입하였다. CFD 기법 및 음향상사법을 적용한 복합 전산공력음향학 기법을 적용하여 제안한 저소음 사보니우스형 풍력터빈의 방사 소음을 수치적으로 예측하였고, 기존의 형상과 비교를 통해 소음 저감 효과를 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제25권5호
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• pp.606-612
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• 2012

Insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore the significance of IGFBP-5 in cells of the Inner Mongolian Cashmere goat (Capra hircus), IGFBP-5 gene complementary DNA (cDNA) was amplified by reverse transcription polymerase chain reaction (RT-PCR) from the animal's fetal fibroblasts and tissue-specific expression analysis was performed by semi-quantitative RT-PCR. The gene is 816 base pairs (bp) in length and includes the complete open reading frame, encoding 271 amino acids (GenBank accession number JF720883). The full cDNA nucleotide sequence has a 99% identity with sheep, 98% with cattle and 95% with human. The amino acids sequence shares identity with 99%, 99% and 99%, respectively. The bioinformatics analysis showed that IGFBP-5 has an insulin growth factor-binding protein homologues (IB) domain and a thyroglobulin type-1 (TY) domain, four protein kinase C phosphorylation sites, five casein kinase II phosphorylation sites, three prenyl group binding sites (CaaX box). The IGFBP-5 gene was expressed in all the tested tissues including testis, brain, liver, lung, mammary gland, spleen, and kidney, suggesting that IGFBP-5 plays an important role in goat cells.

• 복식문화연구
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• 제21권1호
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• pp.1-16
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• 2013

This study analyzed the characteristics of Korean traditional plain weave excavated from Sang-go period to Joseon Dynasty. To do this, this study classified the Korean traditional plain weave into fiber types(cotton, hemp & ramie, plain weaved silk), analyzed and compared the thickness, twist type of yarns and density by times. First, in characteristics of cotton, the average and maximum density of Joseon Dynasty were higher than those of Goryeo, twist type was mainly s-twist and the density of warp was higher than that of weft. Second, the maximum density of hemp & ramie was found in era of Three Kingdoms of Korea. In common characteristics of hemp & ramie, twist type was mainly s-twist(sometimes non-twist) to the Three Kingdoms of Korea and was changed into non-twist from the Goryeo. The density of warp was higher than that of weft in common. Generally, the average density of ramie was higher than that of hemp. Third, in the characteristics of plain weaved silk, twist type was mainly non-twist(sometimes s and z-twist) from Sang-go period to Joseon Dynasty. Warp-faced ribbed tabby was excavated in Goryeo, the average density of warp-faced ribbed tabby was higher than that of other fiber types plain weave. Generally, in all fiber types, the density of warp was higher than that of weft.