• Toxicological Research
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• 제20권1호
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• pp.71-82
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• 2004

Changes in cell cycle control in the lungs and liver of the B6C3F1 mice (20 males per each group) exposed to ozone (0.5 ppm), 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK, 1.0 mg/kg), and dibutyl phthalate (DBP, 5,000 ppm) after 52 weeks were examined through Western, Northern blot, and immunohistochemistry based on alterations in protein expression levels of G1/S checkpoints (cyclin D1, cyclin E, and PCNA), G2/M checkpoints (cyclin B1, cyclin G, and cyclin A), negative regulators (p53, p21, GADD45, and p27), and positive regulator (mdm2). Expression levels of cyclins D1, E, G, PCNA, mutant p53, and mdm2 proteins were higher in the lungs and livers treated with combination of toxicants than in those treated with ozone only. Expression levels of the wild-type and mutant p53, p21, GADD45, p27, and mdm2 proteins and mRNAs were higher in toxicant-treated groups than those of the control. Immunohistochemical analysis revealed staining intensities of the PCNA, cyclin D1, c-myc and mdm2 protein- treated lungs and livers were stronger than those of the control group. Our results showed that combined treatment of ozone with NNK/DBP altered the cell cycle control through instability of the wild-type p53 gene. Such pivotal p53-mediated cell cycle alterations may be responsible for the toxicity observed under our experimental condition. These results may be applied to risk assessment of mixture-induced toxicity.

• 한국세라믹학회지
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• 제35권10호
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• pp.1061-1069
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• 1998

The thermal instability of Al2TiO5 Ceramics was contrlled by solid solution with MgO SiO2 and ZrO2 through electrofusion in an arc furnace. The thermal expansion properties of Al2TiO5 composites show the hysteresis due to the strong anisotropy of The crystal axes of these material. These phenomena are ex-plained by the opening and closing of microcracks. The difference in microcracking temperatures e.g 587.6(ATG2), 405.9(ATG3) and 519.7$^{\circ}C$(ATG4) is caused by the difference in grain size and stabilizer type. The thermal shock behaviour under cyclic conditions between 750-1400-75$0^{\circ}C$ show no change in mi-crostructure and phase assemblage for all three stabilized specimens. After the thermal loading test at 110$0^{\circ}C$ for 100hrs. ATG1 and ATG2 materials decomposes completely to its components corundum and ru-tile in both cases. However with approximatelly 20% retention of the Al2TiO5 Thus in order to prevent decomposition of the stabilized material in the critical temperature range 800-130$0^{\circ}C$ it must be traversed within a short period of time.

• BMB Reports
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• 제41권10호
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• pp.716-721
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• 2008

To explore the effects of deregulated expression of the EBNA1 binding protein 2 (EBP2) on cell growth, we generated human HEK293 stable clones constitutively expressing an EBP2-EGFP fusion protein. We found both RNA and protein levels of cyclin E1, a dominant oncoprotein, were elevated in the EBP2- EGFP stable clones. These findings were confirmed by flow cytometry bivariate analysis of cyclin expression versus DNA content. Moreover, the increase in p21 expression and the specific phosphorylation at Ser1981 of ATM and Ser15 of p53 were also observed in these stable clones, and these observations may explain the failure to observe an increase in Cdk2 kinase activity. In addition, after one year of passage culture, the EBP2-EGFP stable clones tended to lose 4 to 5 chromosomes per cell when compared to that of control cells. All of these findings provide a possible link between deregulated expression of EBP2 and tumor development.

• 한국수소및신에너지학회논문집
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• 제22권1호
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• pp.92-99
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• 2011

Barium cerate ($BaCeO_3$) related perovskite ceramics currently dominate the high-temperature proton conductor field. Unfortunately, these materials have very stringent environmental limitations necessitating the costly and complex conditioning or cleaning of the application feed-gas. Commercial realization has been hampered, in part, because of the reactivity of $BaCeO_3$ with $CO_2$, and to some extent $H_2O$. And sintered $BaCeO_3$ decomposed at a rate comparable to the powder samples. In this article, the chemical stability and the structural changes of $BaCe_{0.9-X}Y0.1La_XO_{3-\delta}$ (X=0, 0.1, 0.2) have been systematically investigated in the atmosphere containing carbon dioxide ($CO_2$) and water vapor ($H_2O$). The sintering characteristics were studied in $1600^{\circ}C$, sintered pellets disintegrate and decompose upon contacting boiling water on the surface only.

• Journal of Korean Neurosurgical Society
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• 제29권2호
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• pp.280-285
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• 2000

Posterior transarticular screw fixation for atlantoaxial instability due to trauma or rheumatoid arthritis provides immediate rigid fixation of the C1-2 vertebral segment while preserving motion between the occiput and C1. This technique provides more resistance to translational and rotational forces than wiring technique. However, the technique of transarticular screw fixation is inherently demanding because of the complex anatomy of the occipitocervical region and vertebral artery(VA) at risk for arterial damage. VA injury may lead to serious subsequent neurological deficits and possibly death from bilateral VA injury. We report a case of a vertebral artery-to-epidural venous plexus fistula after posterior transarticular screw fixation which was treated with balloon occlusion.

• 한국수산과학회지
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• 제20권6호
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• pp.484-488
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• 1987

단순만곡형과 슈퍼-V형 모형 전개판의 뒷전 쪽에 각각 flap을 부착하고, 회류수조에서 진행각도와 유속에 따른 항력과 전개력을 측정하여 유체역학적인 전개성능을 분석한 결과는 다음과 같다. 1. flap을 부착한 모형 전개판의 전개력계수 $C_L$은 flap각도 $30^{\circ}$와 $50^{\circ}$일 때 단순만곡형은 최대유효진행각도 $\alpha_{max}=25^{\circ}$ 1.75 정도이었고, 슈퍼-V형은 $\alpha_{max}=20^{\circ}$에서 1.80정도로 flap을 부착하지 않은 경우보다 $20\~30\%$ 정도 $C_L$이 증가하였다. 2. 항력계수 $C_D$는 $\alpha_{max}$ 일 때 flap각도에 관계없이 단순만곡형과 슈퍼-V형 모두가 0.5 정도로 거의 차이가 없었고, flap을 부착하지 않은 경우보다는 약간 $C_D$가 증가하였다. 3. 유체효율 $C_L/C_D$는 flap각도 $30^{\circ}$일 경우가 $50^{\circ}$일 경우보다 약간 높았으나, $\alpha_{max}$일 때는 거의 비슷하였으며, flap를 부착하지 않은 경우보다는 악간 높은 경향을 보였다.

• 한국정밀공학회지
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• 제14권5호
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• pp.108-117
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• 1997

To prove the stability and the effectiveness of the robust non-linear friction control suggested and proved analytically in the previous paper, the describing function analysis is introduced. The instability of the Southward's nonlinear friction compensation for a digital position control and the improvement of phase margin of the robust nonlinear friction compensation are verified qualitatively through the describing function analysis. Those controls are applied to a single-axis digital servo driving experimental setup which has inherent stick-slip friction and experimental results confirm the results obtained in and the effectiveness of the robust nonlinear friction compensation for a digital position control.

• Journal of Korean Neurosurgical Society
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• 제64권5호
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• pp.837-842
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• 2021

An atlantoaxial subluxation from the unstable Os odontoideum by the failure of proper integrations between the embryological somites might be a commonly reported pathology. However, its suspicious origin or paralleled occurrence with other congenital anomalies of vertebral body might be a relatively rare phenomenon. The authors present two cases, who simply presented with clinical signs of prolonged, intractable cervicalgia without any neurological deficits, revealed this rare feature of C1-2 subluxation from the unstable, orthotropic type of Os odontoideum that coincide with congenitally fused cervical vertebral bodies between C2-3. Surprisingly, in one case, when traced from the lower cervical down to the thoracic-lumbar levels during the preoperative work-up process, was also compromised with multi-level butterfly vertebrae formations. Presented cases highlight the association of various congenital vertebrae anomalies and the rationale to fuse only affected joints.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1987년도 전기.전자공학 학술대회 논문집(I)
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• pp.473-476
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• 1987

Low temperature phosphorus-nitride CVD was newly developed for a high quality gate insulator on InP substrate. This film showed the Poole-Frenkel type conduction in high electric field with resistivity higher than $1{\times}10^{14}$ ohm-cm near the electric field of $1{\times}10^7\;volt/cm$. The C-V hysteresis width was very small as 0.17 volt. The density of interface trap states was $2{\times}10^{11}cm^{-2}ev^{-1}$ below the conduction band edge of InP substrate. Effective electron mobility was about $1200-1500\;cm^2/Vsec$ and showed the instability of PN-InP MISFETs drain current reduced less than 10 percent for the period $0.5-10^3sec$.

• 한국미생물·생명공학회지
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• 제22권6호
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• pp.614-620
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• 1994

The effects of growth temperature and nutritional components on the synthesis of poly-3-hydroxybutyric acid, PHB, by filamentation-suppressed recombinant Escherichia coli XL1-Blue (pSYL107) were studied. After culturing XL1-Blue(pSYL107) for 48 hours in complex medium at 30$\circ$C, 7Al g/l of PHB could be obtained with the PHB content and PHB yield of 82% and 0.371 g PHB/g glucose, respectively. Lower concentration of PHB(3.2 g/l) was obtained when cultu- red at 37$\circ$C, which seemed to be due to the instability of this strain having amplified FtsZ activity. The PHB concentration of 3.75 g/l was obtained after culturing 60 hours in R medium supplemen- ted with 20 g/l glucose at 30$\circ$C, which was more than twice higher than that obtained with XL1-Blue(pSYL105). This suggested that the enhancement of PHB synthesis by suppressing filamenta- tion was more significant in a defined medium than complex medium. PHB synthesis could be further enhanced by supplementing a small amount of various complex nitrogen sources. When 5 g/l of beef extract was added to a defined medium, PHB concentration, PHB content, and PHB yield obtained after 60 hours of cultivation at 30$\circ$C were 7.46 g/l, 86%, and 0.375 g PHB/g glucose,respectively.