• 한국미생물학회:학술대회논문집
• /
• 한국미생물학회 2007년도 International Meeting of the Microbiological Society of Korea
• /
• pp.120-122
• /
• 2007

All living organisms use numerous signal-transduction pathways to sense and respond to their environments and thereby survive and proliferate in a range of biological niches. Molecular dissection of these signalling networks has increased our understanding of these communication processes and provides a platform for therapeutic intervention when these pathways malfunction in disease states, including infection. Owing to the expanding availability of sequenced genomes, a wealth of genetic and molecular tools and the conservation of signalling networks, members of the fungal kingdom serve as excellent model systems for more complex, multicellular organisms. Here, we employed Cryptococcus neoformans as a model system to understand how fungal-signalling circuits operate at the molecular level to sense and respond to a plethora of environmental stresses, including osmoticshock, UV, high temperature, oxidative stress and toxic drugs/metabolites. The stress-activated p38/Hog1 MAPK pathway is structurally conserved in many organisms as diverse as yeast and mammals, but its regulation is uniquely specialized in a majority of clinical Cryptococcus neoformans serotype A and D strains to control differentiation and virulence factor regulation. C. neoformans Hog1 MAPK is controlled by Pbs2 MAPK kinase (MAPKK). The Pbs2-Hog1 MAPK cascade is controlled by the fungal "two-component" system that is composed of a response regulator, Ssk1, and multiple sensor kinases, including two-component.like (Tco) 1 and Tco2. Tco1 and Tco2 play shared and distinct roles in stress responses and drug sensitivity through the Hog1 MAPK system. Furthermore, each sensor kinase mediates unique cellular functions for virulence and morphological differentiation. We also identified and characterized the Ssk2 MAPKKK upstream of the MAPKK Pbs2 and the MAPK Hog1 in C. neoformans. The SSK2 gene was identified as a potential component responsible for differential Hog1 regulation between the serotype D sibling f1 strains B3501 and B3502 through comparative analysis of their meiotic map with the meiotic segregation of Hog1-dependent sensitivity to the fungicide fludioxonil. Ssk2 is the only polymorphic component in the Hog1 MAPK module, including two coding sequence changes between the SSK2 alleles in B3501 and B3502 strains. To further support this finding, the SSK2 allele exchange completely swapped Hog1-related phenotypes between B3501 and B3502 strains. In the serotype A strain H99, disruption of the SSK2 gene dramatically enhanced capsule biosynthesis and mating efficiency, similar to pbs2 and hog1 mutations. Furthermore, ssk2, pbs2, and hog1 mutants are all hypersensitive to a variety of stresses and completely resistant to fludioxonil. Taken together, these findings indicate that Ssk2 is the critical interface protein connecting the two-component system and the Pbs2-Hog1 pathway in C. neoformans.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권1호
• /
• pp.138-148
• /
• 2018

Objective: Fusarium mycotoxins deoxynivalenol (DON) and zearalenone (ZEN), common contaminants in the feed of farm animals, cause immune function impairment and organ inflammation. Consequently, the main objective of this study was to elucidate DON and ZEN effects on the mRNA expression of pro-inflammatory cytokines and other immune related genes in the kidneys of piglets. Methods: Fifteen 6-week-old piglets were randomly assigned to three dietary treatments for 4 weeks: control diet, and diets contaminated with either 8 mg DON/kg feed or 0.8 mg ZEN/kg feed. Kidney samples were collected after treatment, and RNA-seq was used to investigate the effects on immune-related genes and gene networks. Results: A total of 186 differentially expressed genes (DEGs) were screened (120 upregulated and 66 downregulated). Gene ontology analysis revealed that the immune response, and cellular and metabolic processes were significantly controlled by these DEGs. The inflammatory stimulation might be an effect of the following enriched Kyoto encyclopedia of genes and genomes pathway analysis found related to immune and disease responses: cytokine-cytokine receptor interaction, chemokine signaling pathway, toll-like receptor signaling pathway, systemic lupus erythematosus (SLE), tuberculosis, Epstein-Barr virus infection, and chemical carcinogenesis. The effects of DON and ZEN on genome-wide expression were assessed, and it was found that the DEGs associated with inflammatory cytokines (interleukin 10 receptor, beta, chemokine [C-X-C motif] ligand 9, CXCL10, chemokine [C-C motif] ligand 4), proliferation (insulin like growth factor binding protein 4, IgG heavy chain, receptor-type tyrosine-protein phosphatase C, cytochrome P450 1A1, ATP-binding cassette sub-family 8), and other immune response networks (lysozyme, complement component 4 binding protein alpha, oligoadenylate synthetase 2, signaling lymphocytic activation molecule-9, ${\alpha}$-aminoadipic semialdehyde dehydrogenase, Ig lambda chain c region, pyruvate dehydrogenase kinase, isozyme 4, carboxylesterase 1), were suppressed by DON and ZEN. Conclusion: In summary, our results indicate that high concentrations of DON and ZEN suppress the inflammatory response in kidneys, leading to potential effects on immune homeostasis.

• 정보처리학회논문지C
• /
• 제13C권7호
• /
• pp.859-864
• /
• 2006

웹의 개방성은 사람들로 하여금 언제, 어디서든 손쉽게 유용한 정보를 획득할 수 있게끔 하였다. 하지만 인터넷은 유용한 정보의 손쉬운 활용이라는 순기능과 더불어 사회적으로 통제를 필요로 하는 유해한 정보 역시 인터넷을 이용하는 이용자들에게 무차별적으로 제공함으로써 역기능을 발생시키고 있다. 성인 컨텐츠 같은 정보들은 모든 사용자들, 특히 청소년들에게 악영향을 미칠 수 있다. 또한, 변태적인 성인 사이트들이 담고 있는 컨텐츠들은 성인들의 정신 건강에도 해를 미치게 된다. 한편, 인터넷은 전 세계적으로 연결된 개방망이므로 유해정보 제공자를 각국의 법적, 제도적 장치를 이용하여 규제하는데 한계가 있다. 또한, 유해 사이트, 유해성 스팸 메일, P2P 등 다양한 경로를 통해 유해 정보를 접할 수 있기 때문에, 어떤 시스템에 특화된 유해정보 분류기술을 개발하는 것은 바람직하지 않다. 따라서, 유해정보의 내용 자체에 기반하여 유해 여부를 자동으로 판별할 수 있는 유해정보 판별 핵심 기술의 연구 및 개발의 중요성이 점차 부각되고 있다. 이에 본 논문에서는 내용 기반 기술을 이용한 효율적인 유해 웹 문서 텍스트 판별 시스템을 제시한다.

• Journal of Microbiology and Biotechnology
• /
• 제17권3호
• /
• pp.496-510
• /
• 2007

This paper describes the use of a discrete mathematical model to represent the basic mechanisms of regulation of the bacteria E. coli in batch fermentation. The specific phenomena studied were the changes in metabolism and genetic regulation when the bacteria use three different carbon substrates (glucose, glycerol, and acetate). The model correctly predicts the behavior of E. coli vis-a-vis substrate mixtures. In a mixture of glucose, glycerol, and acetate, it prefers glucose, then glycerol, and finally acetate. The model included 67 nodes; 28 were genes, 20 enzymes, and 19 regulators/biochemical compounds. The model represents both the genetic regulation and metabolic networks in an integrated form, which is how they function biologically. This is one of the first attempts to include both of these networks in one model. Previously, discrete mathematical models were used only to describe genetic regulation networks. The study of the network dynamics generated 8 $(2^3)$ fixed points, one for each nutrient configuration (substrate mixture) in the medium. The fixed points of the discrete model reflect the phenotypes described. Gene expression and the patterns of the metabolic fluxes generated are described accurately. The activation of the gene regulation network depends basically on the presence of glucose and glycerol. The model predicts the behavior when mixed carbon sources are utilized as well as when there is no carbon source present. Fictitious jokers (Joker1, Joker2, and Repressor SdhC) had to be created to control 12 genes whose regulation mechanism is unknown, since glycerol and glucose do not act directly on the genes. The approach presented in this paper is particularly useful to investigate potential unknown gene regulation mechanisms; such a novel approach can also be used to describe other gene regulation situations such as the comparison between non-recombinant and recombinant yeast strain, producing recombinant proteins, presently under investigation in our group.

• 대한의용생체공학회:의공학회지
• /
• 제41권2호
• /
• pp.107-119
• /
• 2020

The deep neural networks (DNN) that can replicate the behavior of the human expert who recognizes the characteristics of ECG waveform have been developed and studied to analyze ECG. However, although the existing DNNs can not provide the explanations for their decisions, those trials have attempted to determine whether patients have certain diseases or not and those decisions could not be accepted because of the absence of relating theoretical basis. In addition, these DNNs required a lot of training data to obtain sufficient accuracy in spite of the difficulty in the acquisition of relating clinical data. In this study, a small-sized continuous data processing DNN (C-DNN) was suggested to determine the simple characteristics of ECG wave that were not required additional explanations about its decisions and the C-DNN can be easily trained with small training data. Although it can analyze small input data that was selected in narrow region on whole ECG, it can continuously scan all ECG data and find important points such as start and end points of P, QRS and T waves within a short time. The star and end points of ECG waves determined by the C-DNNs were compared with the results performed by human experts to estimate the accuracies of the C-DNNs. The C-DNN has 150 inputs, 51 outputs, two hidden layers and one output layer. To find the start and end points, two C-DNNs were trained through deep learning technology and applied to a parameter acquisition algorithms. 12 lead ECG data measured in four patients and obtained through PhysioNet was processed to make training data by human experts. The accuracy of the C-DNNs were evaluated with extra data that were not used at deep learning by comparing the results between C-DNNs and human experts. The averages of the time differences between the C-DNNs and experts were 0.1 msec and 13.5 msec respectively and those standard deviations were 17.6 msec and 15.7 msec. The final step combining the results of C-DNN through the waveforms of 12 leads was successfully determined all 33 waves without error that the time differences of human experts decision were over 20 msec. The reliable decision of the ECG wave's start and end points benefits the acquisition of accurate ECG parameters such as the wave lengths, amplitudes and intervals of P, QRS and T waves.

• 전기전자학회논문지
• /
• 제2권2호
• /
• pp.255-262
• /
• 1998

The neural network can roughly be classified as the specialized control, indirect control and general schemes. Neural network is adopted for MPPT of solar array. And back propagation algorithm also is used to train neural network controller. We investigate the possibilities of $P_{max}$ control using the neural networks, and then we also examine about operating the solar cell at an optimal voltage comprise of temperature compensated voltage with boost converter. Proposed boost converter of MPPT system is studied by simulation and is implemented by using a microprocessor(80c196kc) which controls duty ratio of the boost converter.

• Journal of Communications and Networks
• /
• 제9권1호
• /
• pp.67-74
• /
• 2007

The performance of dual diversity receivers operating over correlated Ricean fading channels is analyzed. Using a previously derived rapidly converging infinite series representation for the bivariate Ricean probability density function, analytical expressions for the statistics of dual-branch selection combining, maximal-ratio combining, and equal-gain combining output signal-to-noise ratio (SNR) are derived. These expressions are employed to obtain novel analytical formulae for the average output SNR, amount of fading, average bit error probability, and outage probability. The proposed mathematical analysis is used to study various novel performance evaluation results with parameters of interest the fading severity, average input SNRs, and the correlation coefficient. The series convergence rate is also examined verifying the fast convergence of the analytical expressions. The accuracy of most of the theoretical performance evaluation results are validated by means of computer simulations.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 2007년도 제38회 하계학술대회
• /
• pp.1251-1252
• /
• 2007

유류비용의 증가, 환경보존, 기존 에너지원의 분산전원으로서의 신재생에너지에 대한 관심과 연구, 개발, 보급등에 대한 관심이 고조되고 있는 현실에서 신재생에너지원에서 발생되는 출력 및 발전환경에 대한 관리 기법의 중요성은 나날히 증가하고 있다. 본 논문에서는 기존의 신재생에너지 시스템의 관리 현황을 분석하고 새로운 방식을 제안함으로서 신재생에너지에서 출력되는 데이터의 효율적인 관리가 가능하도록 하는 네트워크 통합관리 기능에 대해 논하고자 한다. 본 논문에서는 효율적인 관리 툴의 프로그래밍을 위해 NI사의 LabVIEW S/W를 사용하였으며 각각의 데이터 수집을 위해 DAQ와 측정 트랜듀서를 사용하였다.

• International Journal of Computer Science & Network Security
• /
• 제23권11호
• /
• pp.73-76
• /
• 2023

We introduce DCNN and DRAE appraoches for compression of medical videos, in order to decrease file size and storage requirements, there is an increasing need for medical video compression nowadays. Using a lossy compression technique, a higher compression ratio can be attained, but information will be lost and possible diagnostic mistakes may follow. The requirement to store medical video in lossless format results from this. The aim of utilizing a lossless compression tool is to maximize compression because the traditional lossless compression technique yields a poor compression ratio. The temporal and spatial redundancy seen in video sequences can be successfully utilized by the proposed DCNN and DRAE encoding. This paper describes the lossless encoding mode and shows how a compression ratio greater than 2 (2:1) can be achieved.

• 한국주거학회논문집
• /
• 제25권3호
• /
• pp.115-121
• /
• 2014

This paper draws the home into the stream of information and communications technology (ICT) development. Considering that homes and our home life are constantly affected by persistent change influenced by ICT, it is remarkable that ICT has been relatively neglected as an object of research in the field of housing studies. This study provides an overview of conceptual movements in ICT/home relationships and their design and social impact through a critical discourse analysis. The conceptual movements must be considered by professionals in the field of housing and built environment to stay attuned to changes in the 21st Century. Diverse debates took place in the discourse about ICT in relation to home. The stream of thought was started by futurists who saw the electronic cottage as a solution to the social problems caused by the separation of work and home life in industrial cities. ICT has now entered a new phase, with WiFi P2P networks and location-based social media that encourage sharable homes. Based on the analysis of the discourse on ICT and home, we drew from three issues related to the paradigm shifts in homes in the context of ICT development to provide future directions for housing studies: from space programming to time scheduling, from individual ownership to shared access, and from live+work dwelling to live+work+community.