This study was conducted to investigate whether 1) photosynthetic pigments(chlorophylls and carotenoids) are formed in dodder plant(Cuscuta australis), 2) there are any characteristics in the pigment biosynthesis, compared to that of other normal plants, and 3) dodder responds to some herbicides having target site on chloroplast. 1. Chlorophyll content of dodder tendrill grown under a natural daylight was 9 times and 50 times lower than that of field bindweed stem and leaf, respectively. 2. The photosynthetic pigment contents varied in different tissues, being higher in a apical region than in a lower region of seedling or tendrill. Chlorophyll wasn't almost observed below the 4th internode from the upper. 3. Pigment contents were greatly dependent on light intensity so that there were 4 to 6 times difference among light conditions. When the shoot containning low pigment contents under natural light, was incubated in growth chamber with various light intensities, the pigment contents were increased by 3 times of initial contents at about 97${\mu}E$$m^{-2}s^{-1}$PAR. While the change in pigment contents was not observed at above 450${\mu}E$$m^{-2}s^{-1}$PAR 4. Exogenous supply of 5mM 5-aminolevulinic acid increased protochlorophyllide by 7 times and 1.4 times in the etiolated shoot from field bindweed rhizome and in dodder stem, respectively, showing that dodder relatively has a low response to 5-aminolevulinic acid. 5. Pigment loss was observed in the treatment of paraquat, norflurazon, oxyfluorfen and diuron, and protoporphyrin IX was accumulated by oxyfluorfen as in normal plants Based on above results, several chracteristics of pigment biosynthesis in dodder seem to be summerized as follows. Photosynthetic pigment biosynthesis in Cuscuta australis runs even in low level. The pigment contents is differentially distributed in different regions and their contents seem significantly to be controlled by light intensities. Especially, chloroplast rapidly tends to degenerate with the development of tissue. Some herbicides having target site on chloroplast induce damage to dodder stem but are unlikely to control it well in field, except paraquat, due to low chloroplast activity and parasitic mode of nutrition.
Seo, Bo Gil;Yoo, Myung Hwan;Shim, Jae Won;Shim, Jung Yeon;Jung, Hye Lim;Park, Moon Soo;Kim, Deok-Soo
Clinical and Experimental Pediatrics
/
v.49
no.5
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pp.533-538
/
2006
Purpose : The headache, one of the symptoms of meningitis, is related to abrupt elevation of intracranial pressure(ICP) or stimulation of intracranial nociceptive structure. However, in cases of mild elevation of ICP or normal findings of cerebrospinal fluid(CSF) analysis, patients sometimes complain of headaches. Therefore, other pathways may contribute to the occurrence of headaches in aseptic meningitis or meningismus. We intend to investigate the role of substance P(SP) and calcitonin gene-related peptide(CGRP) in aseptic meningitis or meningismus. Methods : We measured leukocyte count, the concentration of protein and glucose in CSF and ICP of patients with meningeal irritation sign. We also measured SP and CGRP levels by using immunoassay. We analyzed the relationship between the presence of headache and the value of SP and CGRP. Results : The concentrations of CGRP($18.8{\pm}10.5ng/mL$) in CSF and ICP($14.8{\pm}4.5cmH_2O$) in aseptic meningitis group were significantly higher than in those($14.1{\pm}7.4ng/mL$ and $12.0{\pm}5.1cmH_2O$, respectively) of the meningismus group(P<0.05). There was no significant difference in the SP levels between the two groups. In the aseptic meningitis group, the concentrations of SP and CGRP were significantly higher in the normal ICP group than in the elevated ICP group(P<0.05). Conclusion : Headaches in children with aseptic meningitis or meningismus is considered to be related to the elevation of the CSF levels of SP and CGRP.
In Summer 2008, a multidisciplinary survey was conducted onboard R/V Haeyang 2000 to understand plankton response to the three distinct physico-chemical settings that developed in the Ulleung Basin of the East Sea. Baseline settings of hydrographic conditions included the presence of the thin (<20 m) Tsushima Surface Water (TSW) on top of the Tsushima Middle Water (TMW). It extends from the Korea Strait to $37^{\circ}N$ along the $130^{\circ}E$ and then turns offshore and encompasses the relatively saline (T>$26^{\circ}C$, S>33.7) Ulleung Warm Eddy surface water centered at $36.5^{\circ}N$ and $131^{\circ}E$. A relatively colder and saline water mass appeared off the southeastern coast of Korea. It was accompanied by higher nutrient and chlorophyll-a concentrations, suggesting a coastal upwelling. Most of the offshore surface waters support low phytoplankton biomass (0.3 mg chl-a $m^{-3}$). A much denser phytoplankton biomass (1-2.3 mg $m^{-3}$) accumulated at the subsurface layer between 20-50 m depth. The subsurface chlorophyll-a maximum (SCM) layer was closely related to the nutricline, suggesting an active growth of phytoplankton at depth. The SCM developed at shallow depth (20-30 m) near the coast and deepened offshore (50-60 m). A fucoxanthin/zeaxanthin ratio was high in coastal waters while it was low in offshore waters, which indicated that diatoms dominate coastal waters while cyanobacteria dominate offshore waters. The community structure and biomass of phytoplanktonare closely related to nitrogen availability. Zooplankton biomass was higher in the coastal region than in the offshore region while species richness showed an opposite trend. Zooplankton community structure retained a coastal/offshore contrast. These suggest that summer hydrography is a stable structure, lasting long enough to allow a hydrography-specific plankton community to evolve.
Local K-index is an indicator representing local geomagnetic activity in every 3 hour. For estimation of the local K-index, a reasonable determination of solar quiet curve (undisturbed daily variation of geomagnetic field) is quiet essential. To derive the solar quiet curve, the FMI method, which is one of representative algorithms, uses horizontal components (H and D) of 3 days magnetometer data from the previous day to the next day for a specific day. However, this method is not applicable to real time forecast since it always requires the next day data. In this study, we have devised a new method to estimate local K-index in near real-time by modifying the FMI method. The new method selects a recent quiet day whose $K_p$ indices, reported by NOAA/SEC are all lower than 3, and replace the previous day and the next day data by the recent quiet day data. We estimated 2,672 local K indices from Gyeongzu magnetometer in 2003, and then compared the indices with those from the conventional FMI method. We also compared the K indices with those from Kakioka observatory. As a result, we found that (1) K indices from the new method are nearly consistent with those of the conventional FMI method with a very high correlation (R=0.96); (2) onr local K indices also have a relatively high correlation (R=0.81) with those from Kakioka station. Our results show that the new method can be used for near real-time estimation of local K indices from Gyeongzu magnetometer.
Lee, Ga-Young;Kim, Min Jee;Kim, So Yeon;Lee, Kyung Bok;Oh, Dong Hyun;Cho, Young Ho;Yoo, Yung Choon
Journal of Life Science
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v.30
no.10
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pp.905-911
/
2020
The adjuvant effect of PAMAM dendrimer G4 (PAMAM) on the induction of humoral and cellular immune responses against keyhole limpet hemocyanin (KLH) was examined. Mice were immunized subcutaneously twice at two-week intervals with KLH, with or without PAMAM dendrimer (100 ㎍/mouse), and the mice immunized with KLH+PAMAM showed significantly higher antibody titers against KLH than those immunized with KLH alone. The assay for determining the isotypes of the antibodies showed that PAMAM augmented the KLH-specific antibody titers of IgG1, IgG2a, IgG2b, IgG3, and IgM. In addition, mice immunized twice with KLH+PAMAM followed by a subcutaneous injection of KLH (20 ㎍/site) 7 weeks after the primary immunization exhibited a higher delayed-type hypersensitivity (DTH) reaction than those treated with KLH alone. In an in vitro analysis of T lymphocyte proliferation in response to KLH in week 8, the splenocytes of mice treated with KLH+PAMAM showed significantly higher proliferating activity than those treated with KLH alone, and the culture supernatants of cell cultures from mice immunized with added PAMAM dendrimer showed higher levels of KLH-specific cytokine (IL-4 and IFN-r) production. These results suggest that PAMAM dendrimer G4 possesses a potent immune-adjuvant activity for enhancing both humoral and cell-mediated immunity specific to foreign antigens.
Journal of Practical Agriculture & Fisheries Research
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v.16
no.1
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pp.193-206
/
2014
The objectives of the project are to increase farmers' income through GAP and to reduce the loss of agricultural produce, for which the Korean partner takes a role of transferring needed technologies to the project site. To accomplish the project plan, it is set to implement the project with six components: construction of buildings, installation of agricultural facilities, establishment of demonstration farms, dispatching experts, conducting training program in Korea and provision of equipments. The Project Management Committee and the Project Implementation Team are consisted of Korean experts and senior officials from Department of Agriculture, Myanmar that managed the project systematically to ensure the success of the project. The process of the project are; the ceremony of laying the foundation and commencing the construction of training center in April, 2012. The Ribbon Cutting Ceremony for the completion of GAP Training Center was successfully held under PMC (MOAI, GAPI/ARDC) arrangement in SAl, Naypyitaw on June 17, 2012. The Chairman of GAPI, Dr. Sang Mu Lee, Director General U Kyaw Win of DOA, officials and staff members from Korea and Myanmar, teachers and students from SAl attended the ceremony. The team carried out an inspection and fixing donors' plates on donated project machineries, agro-equipments, vehicles, computers and printer, furniture, tools and so forth. Demonstration farm for paddy rice, fruits and vegetables was laid out in April, 2012. Twenty nine Korean rice varieties and many Korean vegetable varieties were introduced into GAP Project farm to check the suitability of the varieties under Myanmar growing conditions. Paddy was cultivated three times in DAR and twice in SAl. In June 2012, vinyl houses were started to be constructed for raising seedlings and finished in December 2012. Fruit orchard for mango, longan and dragon fruit was established in June, 2012. Vegetables were grown until successful harvest and the harvested produce was used for panel testing and distribution in January 2013. Machineries for postharvest handling systems were imported in November 2012. Setting the washing line for vegetables were finished and the system as run for testing in June 2013. New water tanks, pine lines, pump house and electricity were set up in October 2013.
Three experiments were conducted to determine utilization of Song-Gang(equation omitted) stone as the dietary additive for growth and immune stimulant in juvenile olive flounder. In the feeding trial, four diets were formulated to contain 0, 0.5, 1.0 and 2.0% Song-Gang(equation omitted) stone per kg diet (SGS$_{0}$ , SGS$_{0.5}$, SGS$_{1.0}$ , SGS$_{2.0}$ ). Fish averaging 5.0$\pm$0.04 g (mean$\pm$SD) were fed one of four experimental diets in triplicate groups for 8 weeks. There were no significant differences in weight gain, feed efficiency, protein efficiency ratio, hematosomatic index, condition factor and survival among fish fed all the diets. In chanlenge test, fish were infected by intraperitoneal injection of 0.1 $m\ell$ bacterial suspension with Edwardsielia tarda per fish after the feeding trial. Fish fed SGS$_{0.5}$ diet have a significantly higher cumulative survival rate than did fish fed the other diets (P<0.05). In the anti-mold test, Asprrgiilus niger, Penicillium pinophiltfm, Chaeromium globosum were inoculated with Song-Gang(equation omitted) stone using ASTM G-21 method. The amount of Α. niger, Ρ. pinuphiium. C. globosum didn't increase in Song-Gang(equation omitted) stone for 4 weeks for the test period. Therefore, these results indicate that 0.5% Song-Gang(equation omitted) stone per kg diet could increase immune resistance in juvenile olive flounder and Song-Gang(equation omitted) stone could be used as the anti-mold additive in fish feed.
Korean Journal of Agricultural and Forest Meteorology
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v.14
no.1
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pp.1-10
/
2012
Digital Forecast of the Korea Meteorological Administration (KMA) represents 5 km gridded weather forecast over the Korean Peninsula and the surrounding oceanic regions in Korean territory. Digital Forecast provides 12 weather forecast elements such as three-hour interval temperature, sky condition, wind direction, wind speed, relative humidity, wave height, probability of precipitation, 12 hour accumulated rain and snow, as well as daily minimum and maximum temperatures. These forecast elements are updated every three-hour for the next 48 hours regularly. The objective of this study was to construct Forest Fire Danger Rating Systems on the Korean Peninsula (FFDRS_KORP) based on the daily weather index (DWI) and to improve the accuracy using the digital forecast data. We produced the thematic maps of temperature, humidity, and wind speed over the Korean Peninsula to analyze DWI. To calculate DWI of the Korean Peninsula it was applied forest fire occurrence probability model by logistic regression analysis, i.e. $[1+{\exp}\{-(2.494+(0.004{\times}T_{max})-(0.008{\times}EF))\}]^{-1}$. The result of verification test among the real-time observatory data, digital forecast and RDAPS data showed that predicting values of the digital forecast advanced more than those of RDAPS data. The results of the comparison with the average forest fire danger rating index (sampled at 233 administrative districts) and those with the digital weather showed higher relative accuracy than those with the RDAPS data. The coefficient of determination of forest fire danger rating was shown as $R^2$=0.854. There was a difference of 0.5 between the national mean fire danger rating index (70) with the application of the real-time observatory data and that with the digital forecast (70.5).
Semen quality was compared between the local Katjang and the cross-bred (local Katjang ♀ ${\times}$ German Fawn ♂) bucks. There were on significant genotypic differences in semen characteristics of concentration (first ejaculate : $6.19{\pm}1.30$ -versus $6.33{\pm}1.40{\times}10^9/ml;$second ejaculate: $5.82{\pm}1.10$ - versus $5.68{\pm}1.45{\times}10^9/ml$, for Katjang and the cross-breds, respectively), percentage live (first ejaculate: $77.61{\pm}1.33%$ versus $77.81{\pm}0.53%$; second ejaculate: $81.97{\pm}1.59%$ versus $82.74{\pm}0.96%$, for Katjang and cross-breds, respectively) and percentage of normal sperms (first ejaculate: $12.54{\pm}3.88%$ versus $26.45{\pm}3.83%$; second ejaculate: $38.68{\pm}3.65%$ versus $28.54{\pm}4.38%$, for Katjang and cross-breds, respectively), with the exception of seminal volume and sperm motility. Means of all variables were within the values reported for other goat breeds, In contrast, the differences in semen characteristics between the first and second ejaculations of both genotypes were more distinct, the second ejaculations always had more volume, more normal sperms and better sperm motility but less sperm concentrations. Removing the seminal plasma and replacing it with tris-citrate buffer greatly prolonged the viability of sperms of both genotypes when stored at $5{^{\circ}C}$. Sperm motility seens to be a good indicator of sperm viability. However, the sperms of the corss-bred bucks withstood the washing process better and their swimming abilities were superior ($8.12{\pm}0.46mm/min$) when compared to those of the local Katjang breed ($5.42{\pm}0.49mm/min$). The higher content of calcium ions in their seminal plasma (first ejaculate: $10.5{\pm}0.8$ versus $10.6{\pm}0.8mg/100ml$;second ejaculate: $15.3{\pm}0.8$ versus $16.1{\pm}0.8mg/100ml$, for Katjang and cross-breds, respectively) means that in natural matings the sperms of the cross-breds would be at an advantage compared to those of the local Katjang, since calcium ions reportedly initiate acrosomal reactions.
With the use of Agrobacterium and gene-gun, cold regulated gene (BN115) has been injected in Chrysanthemum leaf disc and transgenic plants have been produced successfully on the selection media containing phytohormone. To determine the presence of the transferred cold regulated gene (BN115) in the transgenic Chrysanthemum, PCR-amplification indicated the presence of that gene. Real-Time PCR for confirmation of the putative transgenic plants was established. The copy number of cold regulated gene (BN115) is extrapolated on the basis of a standard curve. Serial dilutions of known number of gene copies were in triplicates. In this diagram, PCR cycles are plotted against the fluorescence intensity. The cycle at which the fluorescence reaches a threshold cycle is inversely proportional to the starting amount of target DNA.
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