• Journal of fish pathology
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• v.6 no.2
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• pp.177-189
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• 1993

1. For the prophylaxis of ceroidosis Vitamin E and C supplemented diet(POV 90.4mEg/kg) was used for flounder culture during 80 days. 2. The supplementation of Vitamin E and C with our tested concentrations on the high POV diet supported almost normal growth of flounder. It is very much comparable with the abnormal growth of flounder fed the diet of high POV. 3. The best prophylactic effect were appeared the high POV diet supplemented with Vitamin E 1mg/g diet and Vitamin C 2IU/g diet. These results were analyzed by histological observation of tissues.

• Journal of Gastric Cancer
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• v.8 no.2
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• pp.70-78
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• 2008

Purpose: VEGF-C and VEGF-D are angiogenetic factors, and abnormal expression of E-cadherin hasa role in the progression of gastric carcinoma. The aim of this study was to evaluate the relationship between the expression of E-cadherin, VEGF-C and VEGF-D with the presence of lymph node metastases (LNM) using cytokeratin 18 in early gastric cancer (EGC). Materials and Methods: Immunohistochemical staining for E-cadherin, VEGF-C and VEGF-D was performed in 49 EGC patients from March 1997 to December 2002. To evaluate the real extent of LNM, 1,562 lymph nodes from 49 patients were re-examined with the use of cytokeratin 18. Results: Eleven (0.7%) LNM were newly found in 12.2% (n=6) of patients. The real LNM rate was 3.6% in mucosal invasive (m) cancer and 38.1% in submucosal invasive (sm). Stage migration was seen in three patients (6.1%). Abnormal expression of E-cadherin was detected in 36.7% of the patients and expression of VEGF-C and VEGF-D was detected in 16.3% and 36.7% of the patients, respectively. Abnormal expression of E-cadherin was significantly correlated with tumor differentiation (P=0.0103) and Lauren classification (P<0.0001). There was no positive relationship of VEGF-C and VEGF-D expression with the clinicopathological findings for EGC including LNM. However, the frequency of lymph node metastases was significantly higher in patients that demonstrated abnormal expression of E-cadherin with positive immunoreactivity of VEGF-C or VEGF-D (P=0.031). Conclusion: In present study, we could not demonstrate a relationship between the presence of LNM and expression of VEGF-C and VEGF-D in EGC. However, VEGF-C or VEGF-D expression, in addition to the abnormal expression of E-cadherin, was correlated with the real extent of LNM in EGC.

• Journal of the Korean Society of Food Science and Nutrition
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• v.21 no.4
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• pp.414-417
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• 1992

In order to study the addition effect of Leuconostoc mesenteroides and nisin on Escherichia coli and lactic acid bacteria during fermentation and storage of Kimchi, Kimchi was stored at 4$^{\circ}C$ for 7 days and then in-creased the temperature to $25^{\circ}C$. Lactic acid content on Kimchi fermentation at 4$^{\circ}C$ was maintained initial content which was increased upto 0.9% and lactic bacteria was also increased after switching to $25^{\circ}C$. E. coli, on the other hand, was a little decreased from the initial level, but a significant decrease was found for the those Kimchi of Leuconostoc muenteroides added and nisin treated group when the fermentation temperature was switched to $25^{\circ}C$.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2019.11a
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• pp.71-72
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• 2019

In this study, we describe a method of measuring position using the Additional Secondary Factor (ASF) compensation technique of the eLoran concept in the situation where only Loran-C signals are received without eLoran time broadcasting. Next, we describe the result of test performed to verify the method. The test results showed the position accuracy within 20m and we found the possibility of the method. The method is expected to be used to verify user positioning accuracy before improving Loran-C to eLoran.

• The Korean Journal of Emergency Medical Services
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• v.22 no.1
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• pp.73-82
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• 2018

Purpose: The purpose of this study was to compare the tidal volumes and airway pressures of 3 mask-sealing methods (one hand C-E, two hands C-E, and one hand O-E) for ventilation treatment. Methods: The study subjects were 45 paramedic students. Tidal volume was measured for the three sealing methods by setting a ventilator, connecting it to the masks for 2 minutes, and using Respi-trainer software. Results: Regarding general characteristics, the group of men, in upper grades, and with practical training experience and experience and experience in the implementation of bag-valve-mask ventilation provided higher tidal volumes. Regarding physical characteristics, larger hands and greater grip strength correlated with higher tidal volume. Two hands C-E generated the highest tidal volume of $483.78{\pm}34.14mL$, one hand O-E generated $449.59{\pm}51.09mL$ and one hand C-E generated $394.31{\pm}68.95mL$. Conclusion: Means of tidal volumes were statistically significantly different based on mask sealing methods (p<.001). Two hand C-E was performed by the two-persons task and was suggested as the most effective method. For the one-person task, one hand O-E was the more effective method compared to the previous one hand C-E.

• Korean Journal of Heritage: History & Science
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• v.55 no.4
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• pp.278-294
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• 2022

To date, cultural property education has seen rapid quantitative growth due to national and personal needs. However, qualitative growth is lacking. The objectives of cultural property education have not been established, and therefore, even its identity is not clear. The most pressing issue at present in cultural property education is to first set objectives. This study aimed to analyze the objectives of current cultural property education, identify the problems, and set new objectives to meet significant national and personal needs in terms of education. The problems with the objectives of current cultural property education are that the persons interested in the education do not understand the concept of the education objectives clearly and that the objectives do not contain much actual content of the education. Also, the objectives of the education do not take into account the dynamic competencies and interests of the learners and do not satisfy the changes of the times. To solve these problems, new cultural property education, called 'U.V.E.C.,' was offerred. U.V.E.C. education is aimed at understanding cultural properties, recognizing their value, and enjoying them, and at creating culture. The objectives of U.V.E.C. cultural property education were set such that they can be modified flexibly in a learner-centric way with clear and practical format and contents. Based on this direction, stepwise objectives were set including overall objectives, detailed objectives, and practice objectives, and objective cases of each step were proposed. Considering the generality of the education and the distinct characteristics of the cultural properties, the U.V.E.C. education objectives took into account the diversity of behavioral objectives, clearness in statements, the objectives of problem solving, the initiative of learners and openness for expression outcomes. The U.V.E.C. objectives are clear and specific so that teachers can enhance their pedagogical efficiency and learners are able to develop interesting and diversified competencies. In addition, it is expected that the U.V.E.C. objectives will significantly affect objective setting for education on cultural properties which have not been studied widely. Further systemic and specific studies on the contents and methods of the U.V.E.C. education would help to change the overall education on cultural properties and position the field as a new academic area.

• Proceedings of the Ginseng society Conference
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• 1974.09a
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• pp.77-93
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• 1974

The sapogenins of two-and four-year-old A-merican ginseng plants (Panax quinquefolium L.) (Araliaceae) collected in July and September were studied. American ginseng saponins (panaquilins) differ from Korean ginseng (Panax ginseng C. A. Meyer) saponins (ginsenosides). The American ginseng saponins separated and named were panaquilins A, B, C, D, E-l, E-2, E-3, G-l, G-2, (c) and (d). One-dimensional thin-layer chromatography did not completely separate panaquilin mixture and were subject to misinterpretation. The panaquilins were more accurately separated and identified by the two-dimensional thin-layer method established. Some differences in American ginseng saponins were dependent upon the plant age, time of collection, and part extracted. The American ginseng sapogenin components are panxadiol (panaquilins B and C), oleanolic acid (panaquilin D) and panaxatriol (panaquilin G-l). The panaquilins E-l, E-2 and E-3 mixture contains both panaxadiol and panaxatriol. The genins of panaquilins A, (c), (d) and G-2 were not identified. In addition, ${\beta}-sitosterol$ and stigmasterol were identified from the root ether extracts.

• Journal of Nutrition and Health
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• v.31 no.4
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• pp.729-738
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• 1998

Antioxidants such as vitamin C and E may play a preventive role in the development of cancer and coronary heart disease. The status of vitamins C and E may be affected by lifestyle habits such as smoking , drinking, and exercise. These habits can modify the dietary requirements of vitamin C and vitamin E. the purpose of this study was to determine whether Korean young healthy men and women consume vitamins C and E in sufficient quantities relative to their lifestyle habits. Among the participants in this study, 52% of the men and none of the women were smokers. ; 84% of all subjects drank alcohol more than once a week ; and the men exercised more often than the women. The concentrations of serum total , HDL-, and LDL- cholesterol were higher in the women than in the men, but the serum triglyceride concentrations were higher in the men than in the women. The men consumed less satuated fat than the women (p<0.05) . The daily intakes of vitamin C for the men and the women were 47.1mg and 65.6mg, respectively. On the other hand , the daily vitamin E intake was higher in the men (11.8mg) than women(6.9mg). The serum $\alpha$-tocopherol concentrations of all subjects were in a normal range, and in no subjects were they below the minimum value of ranges. However, about 19% of male subjects and 10% of female subjects showed deficient status, although the mean serum vitamin C levels were normal . Lifestyle habits fo the sort mentioned above have little influence on the serum vitamin C and $\alpha$-tocopherol concentrations. The serum $\alpha$-tocopherol concentration had a positive correlation with total fat and alcohol consumption. The serum vitamin C concentration was positively associated with regular exercise, but it was negatively correlated with the number of cigarettes smoked. Meanwhile, the serum lipid persoxide concentration , the indirect index of oxidative stress, was influenced by certain variable such as body mass index , the number of cigarettes smoked , alcohol consumption, energy expenditure, vitamin C intake, and serum ${\gamma}$-tocopherol concentration. Serum lipid peroxide concentration was positively associated with body mass index, the number of cigarette smoked , serum triglyceride , and HDL-cholesterol concentration. In conclusion , the vitamin E requirements of the subjects were met by the Recommended Daily Allowance (RDA) regardless of lifestyle habits. However, serum vitamin C concentrations showed individual variation and was below the normal ranges. Smoking and exercise influenced serum vitamin C concentration. Therefore, a reevaluation of the requirements of vitamin C relative to lifestyle habits is necessary.

• Journal of Photoscience
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• v.9 no.2
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• pp.25-28
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• 2002

The chicken pineal gland has been used for studies on the circadian clock, because it retains an intracellular phototransduction pathway regulating the phase of the intrinsic clock oscillator. Previously, we identified chicken clock genes expressed in the gland (cPer2, cPer3, cBmal1, cBmal2, cCry1, cCry2, and cClock), and showed that a cBMALl/2-cCLOCK heteromer acts as a regulator transactivating cPer2 gene through the CACGTG E-box element found in its promoter. Notably, mRNA expression of cPer2 gene is up-regulated by light as well as is driven by the circadian clock, implying that light-dependent clock resetting may involve the up-regulation of cPer2 gene. To explore the mechanism of light-dependent gene expression unidentified in animals, we first focused on pinopsin gene whose mRNA level is also up-regulated by light. A pinopsin promoter was isolated and analyzed by transcriptional assays using cultured chicken pineal cells, resulting in identification of an 18-bp light-responsive element that includes a CACGTG E-box sequence. We also investigated a role of mitogen-activated protein kinase (MAPK) in the clock resetting, especially in the E-box-dependent transcriptional regulation, because MAPK is phospholylated (activated) in a circadian manner and is rapidly dephosphorylated by light in the gland. Both pulldown analysis and kinase assay revealed that MAPK directly associates with BMAL1 to phosphorylate it at several Ser/Thr residues. Transcriptional analyses implied that the MAPK-mediated phosphorylation may negatively regulate the BMAL-CLOCK-dependent transactivation through the E-box. These results suggest that the CACGTG E-box serves not only as a clock-controlled element but also as a light-responsive element.

• Journal of Microbiology and Biotechnology
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• v.23 no.12
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• pp.1726-1736
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• 2013

Biosynthesis of indole-3-acetic acid (IAA) via the indole-3-pyruvic acid pathway involves three kinds of enzymes; aminotransferase encoded by aspC, indole-3-pyruvic acid decarboxylase encoded by ipdC, and indole-3-acetic acid dehydrogenase encoded by iad1. The ipdC from Enterobacter cloacae ATCC 13047, aspC from Escherichia coli, and iad1 from Ustilago maydis were cloned and expressed under the control of the tac and sod promoters in E. coli. According to SDS-PAGE and enzyme activity, IpdC and Iad1 showed good expression under the control of $P_{tac}$, whereas AspC was efficiently expressed by $P_{sod}$ originating from Corynebacterium glutamicum. The activities of IpdC, AspC, and Iad1 from the crude extracts of recombinant E. coli Top 10 were 215.6, 5.7, and 272.1 nmol/min/mg-protein, respectively. The recombinant E. coli $DH5{\alpha}$ expressing IpdC, AspC, and Iad1 produced about 1.1 g/l of IAA and 0.13 g/l of tryptophol (TOL) after 48 h of cultivation in LB medium with 2 g/l tryptophan. To improve IAA production, a tnaA gene mediating indole formation from tryptophan was deleted. As a result, E. coli IAA68 with expression of the three genes produced 1.8 g/l of IAA, which is a 1.6-fold increase compared with wild-type $DH5{\alpha}$ harboring the same plasmids. Moreover, the complete conversion of tryptophan to IAA was achieved by E. coli IAA68. Finally, E. coli IAA68 produced 3.0 g/l of IAA after 24 h cultivation in LB medium supplemented with 4 g/l of tryptophan.