• Title/Summary/Keyword: C. themocellum

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Cloning and Expression in Escherichia coli of a Cellulase Gene from Clostridium thermocellum (Clostridium thermocellum의 Cellulase 유전자의 Cloning)

  • 하지홍;한성숙;김욱한;이용현
    • Microbiology and Biotechnology Letters
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    • v.15 no.5
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    • pp.346-351
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    • 1987

  • A cellulase gene of Clostridium themocellum was transferred to Escherichia coli by molecular cloning with pBR322. The gene was carried in a Hind III digested DNA sequence of about 1.8 kb. This Rind III fragment expressed activities on carboxymethyl cellulose (CMC) and on filter gaper in E. coli. The expression of clostridial cellulase gene in E. coli was studied and compared with the pro-ducts of cellulase genes in C. themocellum.

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