• Plant Disease and Agriculture
• /
• v.1 no.1
• /
• pp.19-29
• /
• 1995

A microbial product composed of three antagonistic fungal isolates (Aspergillus sp., Penicillium sp. and Trichoderma sp.) and three bacterial isolates (Arthrobacter sp., Bacillus sp., and Pseudomonas sp.) was tested for the control of Pythium blight caused by Pythium sp., brown patch by Rhizoctonia solani (anastomosis group(AG) 1-1) and large patch by R. solani (AG 2-2) of turfgrasses. Cultures of the antagonistic fungi and bacteria varied in the effectiveness in reducing disease severity of Pytium blight and brown patch on bentgrass. The antagonistic fungal and bacterial isolates were mixed and cultured at 20-$25^{\circ}C$ for 3 days in a growth medium, and the diluted solution of the microbial culture was applied under the field conditions after inoculation of the above turfgrass pathogens. The treated turfgrass was incubated at 28$^{\circ}C$ in a growth chamber. In this experiment, Pythium blight was almost completely controlled and brown patch was slightly decreased by the microbial product, while no control was observed in large patch of zoysiagrass. In zoysiagrass treated with the microbial culture, thatch accumulation was notably reduced.

• Journal of Life Science
• /
• v.9 no.6
• /
• pp.737-742
• /
• 1999

The extremely halophilic archaebacterium Haloarcular sp. EH-1 was isolated from solar salts. Haloarcular sp. EH-1 accumulated poly(3-hydroxyalkanoate) (PHA) as intracellular granules. PHA production in batch culture have been studied. The PHA was identified as poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHB/HV) of 3-hydroxybutyric acid and 3-hydroxyvaleric acid by the analysis of GC, IR and NMR. The melting temperature of PHB/HV was 152.46$^{\circ}C$, viscosity was 1.25 ㎗/g, and molecular weight was $1.44 X 10^5.$

• ALGAE
• /
• v.30 no.1
• /
• pp.15-33
• /
• 2015

When the generitype Centroceras clavulatum, a presumed cosmopolitan warm temperate to tropical red alga, was discovered to have a biogeographic distribution limited to the Pacific Ocean using molecular and morphological evidence, the taxonomy in the genus Centroceras was thrown into chaos worldwide. An analysis of what species was, or were, previously identified as C. clavulatum in Bermuda is the focus of the present molecular (COI-5P, rbcL) and morphological study. Two novel species are proposed, C. arcii sp. nov. and C. illaqueans sp. nov., and the distributions of three taxa recently segregated in the 'C. clavulatum complex' of the western Atlantic, C. gasparrinii, C. hyalacanthum, and C. micracanthum, have been expanded to include Bermuda. C. arcii is shown to be morphologically cryptic with C. micracanthum, and remains best distinguished by its COI-5P barcode sequence.

• Microbiology and Biotechnology Letters
• /
• v.30 no.3
• /
• pp.223-229
• /
• 2002

For the research of the natural marine antioxidant, several bacteria were isolated from the coast of jin-Hae in Korea. Among the marine bacteria studied, strain HJ-14, a gram-negative, motile, strait rod, aerobic, and $Na^{+}$ required bacterium showed high activity of 1,1-diphenyl-2-picrylhydrazyl radical scav- enging. The morphological, physiological, and biochemical characteristics of the strain HJ-14 were similar to those of the Alteromonas macleodii ATCC $27126^{T}$ . Thus, it was tentatively identified as Alteromonas sp. HJ-14. The compositions of major fatty acids in cell membrane of Alteromonas sp. HJ-14 were $C_{ 14:0}$ , $ C_{15:0}$ , $C_{16:0}$ and $C_{17:1}$ $_{w8c}$ , which also suggest that it is affiliated with Alteromonas sp. The optimum culture conditions for production of antioxidant materials with Alteromonas sp. HJ-14 were at $25$~$37^{\circ}C$ and pH 6~8. The optimum conditions for the production of antioxidant for carbon, inorganic nitrogen, and sodium chloride sources were 2.5%(w/v) dextrin, 0.5%(w/v) ammonium sulfate, and 2~6%(w/v) sodium chloride, respectively. The hydroxyl radical scavenging ability of Alteromonas sp. HJ-14 broth was 90.03%, which is higher than ascor-bic acid(83.28%) and lower than butylated hydroxyanisole(95.46%) and $\alpha$-tocopherol(97.17%).

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.13 no.6
• /
• pp.39-48
• /
• 2010

The purpose of this study was to analyze air temperature decreasing effects by shading and ventilation at micro-scale experiment plots, especially focused on the Wet Bulb Globe Temperature (WBGT) in outdoor spaces. To monitor the time-serial changes of Dry-bulb Temperature (DT), Globe Temperature (GT) and Relative Humidity (RH) in the wind blocking and shading conditions, Two hexahedral steel frames were established on the open grass field, the dimension of each frame was 1.5m(W)${\times}$1.5m(L)${\times}$1.5m(H). Four vertical side of one frame was covered by transparent polyethylene film to prevent wind passing through (Wind break plot; WP). The top side of the other frame was covered with shading curtain which intercept 95% of solar light and energy (Shading plot; SP). And, Another vertical steel frame without any treatment preventing ventilation and sunlight was set up, which represents natural conditions (Control plot; CP). The major findings were as follows; 1. The average globe temperature (GT) was highest at WP showing $50.94^{\circ}C$ and lowest at SP showing $34.58^{\circ}C$. The GT of natural condition (SP) was $42.31^{\circ}C$ locating the midst between WP and SP. The difference of GT of each plot was about $8-16^{\circ}C$, which means the ventilation and shading has significant effect on decreasing the temperature. 2. WP showed the highest average dry-bulb temperature (DT) of $38.41^{\circ}C$ which apparently differ from SP and CP showing $31.94^{\circ}C$ and $33.15^{\circ}C$ respectively. The DT of SP and CP were nearly the same. 3. The average relative humidity (RH) was lowest at WP showing 15.21%, but SP and CP had similar RH 28.79%, 28.02% respectively. 4. The average of calculated WBGT were the highest at the WP ($27.61^{\circ}C$) and the lowest at the SP ($23.64^{\circ}C$). The CP ($25.49^{\circ}C$) was in the middle of the others. As summery, compared with natural condition (CP), the wind blocking increased about $2.11^{\circ}C$ WBGT, but the shading decreased about $1.84^{\circ}C$ WBGT. So It can be apparently said that the open space with much shading trees, sheltering furnitures and well-delivered wind corridor can reduce useless and even harmful energy for human outdoor activity considerably in outdoor spaces.

• Microbiology and Biotechnology Letters
• /
• v.10 no.3
• /
• pp.185-190
• /
• 1982

A strain of Streptomyces sp. (YS-40) which was able to produce penicillinase, was isolated from soil and the enzymatic characteristics of this enzyme were investigated. The crude enzyme was obtained with the fractionation by 80 % cold acetone. The optimal temperature and pH of this enzyme was 45$^{\circ}C$ and 5.0 respectively. The stable pH range of the enzyme was between pH 5.5 and 8.0 at 37$^{\circ}C$. By heat treatment at 6$0^{\circ}C$ and 8$0^{\circ}C$ for 10 min, the remained relative activities were about 50%, 30% respectively. The activity of the enzyme was inhibited by Cu$^{＋＋}$, $_Mn^{＋＋}$, Zn$^{＋＋}$ but Co$^{＋＋}$, Li$^{＋＋}$, $Ca^{＋＋}$, $Mg^{＋＋}$ $Ba^{＋＋}$ did not affect. Among 11 chemical reagents, ethylenedi aminetetra-acetic acid disodium salt (EDTA-2Na), sodium dodecyl sulfate (SDS) and sodium fluoride inhibited the enzyme activity.

• Journal of Environmental Health Sciences
• /
• v.18 no.2
• /
• pp.92-101
• /
• 1992

Halogen substituted-phenol and analog phenol degrading strains were identified as Aeromonas, Moraxella, and Flavobacterium genus. Optimal degrading condition was generally 50~100 $\mu$M substituted-phenol as carbon source, $NH_4NO_3$ as nitrogen source, 30$\circ$C , and initial pH 7.2. $\rho$-Chlorophenol degrading strain of Aeromonas sp. C4 had biodegradability to the various substituted-phenols. Flavobacterium sp. M9 had substrate specificity to methyl substituted-function. Catechol was cleavaged by catechol 1, 2-dioxygenase in Aeromonas sp. C4, Moraxella sp. N7, and Flavobacterium sp. M9.

• Journal of Microbiology and Biotechnology
• /
• v.14 no.5
• /
• pp.924-931
• /
• 2004

A bacteriocin-producing lactic acid bacterium, which strongly inhibited the Lactobacillus plantarum recognized as an important acid spoilage microorganism in kimchi fermentation, was isolated from kimchi. From morphological, physiological, sugar fermentation, biochemical tests, and l6S rDNA sequencing results, the isolate was identified as an Enterococcus sp. and designated as Enterococcus sp. K25. The bacteriocin produced by Enterococcus sp. K25 inhibited several Gram-positive bacteria, including Lb. plantarum, whereas it did not inhibit Gram-negative bacteria and yeasts. Optimal temperature and pH for the bacteriocin production were $25^\circ{C}$ and 5.5, respectively. Enterococcus sp. K25 was applied to kimchi manufacturing alone and together with other preservatives (i.e., chitosan and fumaric acid). In addition, growth of lactic acid bacteria, pH, and titratable acidity (TA) were measured during aging at $5^\circ{C}$ and $10^\circ{C}$. Inoculation of Enterococcus sp. K25 together with fumaric acid showed the most synergistic effect on extension of kimchi shelf-life. Compared to control (no addition), the treatment prolonged the kimchi shelf-life up to 6 days, whereupon the eight-point TA value recognized as the edible limit was reached.

• Microbiology and Biotechnology Letters
• /
• v.30 no.4
• /
• pp.346-351
• /
• 2002

A bacterium producing the neutral pretense was isolated from soil, and was identified as Bacillus sp. DS-1 by 16S rRNA sequence comparison and biochemical determinations. The production of protease from Bacillus sp. DS-1 was increased 20% and 30% by the additions of 1% glucose and 1% yeast extract, respectively. The optimum pH and temperature for the protease activity were pH 7.0 and 55$^{\circ}C$. Bacillus sp. DS-1 produced a metalloprotease as a major protease in culture medium, since the pretense activity in culture supernatant was inhibited by the presence of 1 mM EDTA significantly.

• Journal of Ecology and Environment
• /
• v.37 no.4
• /
• pp.387-393
• /
• 2014

We propose Cryptonemia asiatica sp. nov. from Korea and Japan. We used molecular analyses of plastid-encoded rbcL and morphological observations to resolve the taxonomic identities of C. lactuca from Korea, C. luxurians from Japan, and C. seminervis from Spain. Specimens of C. lactuca and C. luxurians fell within the same molecular phylogenetic clade (with 100% bootstrap support) and were clearly separated from specimens of C. luxurians collected from the type locality in Brazil. Our analyses demonstrated identical molecular sequences between C. seminervis specimens from Spain and C. lomation specimens from France. Morphological characteristics of the new species, C. asiatica include prominent midribs through the mid thallus, a cortex 4-6 cells thick, and a blade with undulate margins. Molecular evidence indicates that specimens from Korea and Japan previously assigned to C. lactuca and C. luxurians, respectively, should be reassigned to Cryptonemia asiatica. Binomial C. luxurians from Brazil should be resurrected as the independent species of Cryptonemia.