• 한국식품과학회지
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• 제43권4호
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• pp.469-474
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• 2011

본 실험에서는 생강 에탄올 추출물의 극성에 따른 용매 분획을 실시하여 생강의 유용성분을 분석하였으며, 초임계 추출 장치를 이용하여 oil을 추출한 후 남은 박을 다시 에탄올로 추출하여 각각의 총 폴리페놀 및 플라보노이드의 함량과 DPPH, ABTS radical 소거활성을 측정하여 항산화 활성을 비교 검색하였다. 알려진 생강의 유용성분 중 6-gingerol의 함량이 가장 높았으며, n-hexane층에 약 25% 존재하여 6-shogaol 보다 7배 이상 높은 것을 확인했다. 항산화 성분 측정 결과, 생강 박 에탄올 추출물의 총 플라보노이드 함량이 생강 에탄올 추출물보다 높은 경향을 나타내어 생강의 오일 추출 후에 플라보노이드 성분의 용출이 용이해 졌음을 알 수 있었다. DPPH와 ABTS 라디컬 소거능은 초임계 오일에서 높았으며 특히, 45$^{\circ}C$ 이상의 온도 및 200 bar 이상의 조건에서 추출한 오일에서 우수한 소거활성을 나타냈다. 또한, 오일뿐 아니라 박 에탄올 추출물은 온도와 관계없이 압력 100 bar에서 항산화능이 우수하였으며 특히, 55$^{\circ}C$, 100 bar에서 20.17${\pm}$0.83 ${\mu}g/mL$로 강한 소거활성을 확인할 수 있었다. 이는 초임계 오일은 고온, 고압에서 추출 시 많은 양의 항산화 성분이 용출되며, 박 추출물의 경우에는 낮은 압력에서 얻은 박에 많은 양의 항산화 성분이 존재하는 것을 알 수 있다. 이러한 결과로부터, 초임계 추출법이 항산화 성분 추출에 보다 효율적인 방법이며, 생강 특유의 향과 고농도의 6-gingerol을 함유하는 초임계 오일뿐만 아니라 향 성분이 제거된 박 추출물도 높은 항산화 효과를 나타내는 기능성 천연 소재로의 활용이 가능할 것으로 사료되며 향후 다양한 산업적 활용이 기대된다.

• 한국식품영양과학회지
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• 제28권5호
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• pp.997-1002
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• 1999

The aim of the present study was to investigate effects of food preservative addition and changes in composition of herb extract product on the growth of spoilage yeast, Zygosaccharomyces sp. Herbs such as Panax ginseng, Cinnamomum cassia, Lycium chinense, Zyzyphus juiuba and Jingiber officinale were altogether put into water and essence was extracted at 80oC, and then the extract was concentrated at 75oC. The herb extract product was made by adding vitamins, amino acids and honey to the concentrated herb extract. The amount of gas produced from the herb extract product was increased as inoculated cell number increased but decreased as Brix concentration increased. Gases were produced in small amount when incubation was made at 4oC but large amounts of gases were produced at 25 or 40oC of incubation. The gas production and growth of Zygosaccharomyces sp. were measured after browning reaction was induced by heating at 85oC for 12 hours. It appeared that heating treatment did not induce any significant change in the gas production and growth of the cell. The effects of addition of various sugar to the herb extract produce were also invesigated. Amounts of gas production were in the order of glucose>sucrose>oligosaccharide>stevioside. The viable cell count was measured as 6.0$\times$107 CFU/g when glucose was added to the herb extract product. The viable cell counts were 5.0$\times$106, 3.0$\times$103, and 3.0$\times$102 CFU/g in sucrose, oligosaccharide and stevioside added herb extract product, respectively. The amount of gas production from the herb extract product was remarkably reduced by addition of such food preservatives as sodium benzoate and DF 100. TLC(thin layer chromatography) chromatogram of the herb extract showed stability of the herb extract in the above treatments.

• 한국식품저장유통학회지
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• 제19권6호
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• pp.813-817
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• 2012

신선절단 '후지' 사과의 갈변도에 화학물질(ascorbic acid, cystein, sodium chloride, magnesium chloride, calcium chloride, histidine: 1% 용액), 한약재(갈근, 당귀, 산약, 오약, 천궁, 황기: 1% 추출액), 가열(절단과실과 원형과실, $40-60^{\circ}C$ 증류수) 및 코팅(albumin, dextrin, sucrose poly ester, whole soy flour: 0.5-4% 용액) 처리가 미치는 영향을 조사하였다. 각 처리방법별 신선절단 사과의 갈변도는 1% calcium chloride 용액 처리, 황기 추출물 처리, 3% dextrin과 1% whole soy flour 코팅 처리에 의해 가장 낮은 수준을 보였다. 가열처리의 갈변도는 절단과실 처리시에는 $50^{\circ}C$에서, 원형과실 처리시에는 $45^{\circ}C$에서 각각 처리한 시료에서 가장 낮은 수준을 보였다. 이로써 신선절단 사과의 갈변에 대한 한약재 추출물, 가열 및 코팅 처리의 억제 효과와 조건이 확인되었고, 이러한 방법은 화학물질을 부분적으로 대체할 수 있을 것으로 판단된다.

• 혜화의학회지
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• 제5권1호
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• pp.185-198
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• 1996

After evaluation of antithrombotic effect of 40 herbs on platelet aggregation induced by ADP(Adenosine diphosphate), these results were obtained as follows: 1. Crude drugs exerting over 30 % of in Chinemys reevesii (Gray)hibition on platelet aggregation induced by ADP were Ganoderma japonicum (Fr.) Lloyd., Panax ginseng C. A. Mey., Gastrodia elata Bl., Thea sinensis, Chinemys reevesii (Gray), Cuscuta chinensis Lam., Cervus nippon Temminck., Biota orientalis (L.) Endl., Coriolus versicolor, Cinnamomum cassia Presl., Sophora flavescens Ait., Amomum villosum Lour., Carthamus tinctorius L., Rubus chingii Hu., Poria cocos (Schw.) Wolf., Laminana japonica Aresch., Ligustrum lucidum Ait., Angelica sineusis (Oliv.), Cyperus rotundas L., Ginkgo biloba L., Zingiber officinale Rosc., Prunus persica (L.) Batsch., Schizandra chinensis (Turcz.) Baill. and Plantago asiatica L.. 2. Of crude drugs having showed over 50% of inhibitory effect on platelet aggregation, at the concentration of $100{\mu}g/m{\ell}$, the inhibitory rates were 82.2% in Ganoderma japonicum (Fr.) Lloyd., 55% in Panax ginseng C. A. Mey., 50.8% in Gastrodia elata Bl., while at the concentration of $200{\mu}g/m{\ell}$, antithrombotic rates were 89.4% in Ganoderma japonicum (Fr.) Lloyd., 59.2% in Panax ginseng C. A. Mey., 57.9% in Thea sinensis, 52.7% in Gastrodia elata Bl.. These results suggest that the study sholuld be necessary on antithrombotic effect of solvent fractions of Ganoderma japonicum (Fr.) Lloyd., Panax ginseng C. A. Mey., Gastrodia elaha B1. and Thea sinensis and isolation of effective compound from above drugs.

• Natural Product Sciences
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• 제3권1호
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• pp.29-37
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• 1997

Protein Kinase C (PKC) is generally believed to play a central role in signal transduction, cellular growth control, gene expression, and tumor promotion. And it has been suggested that inhibitors of PKC might play important roles for the prevention and treatment of cancer. In order to investigate the possible inhibitors of PKC from natural products, PKC receptor binding assay was performed using bovine brain particulate as a source of PKC and the amount of $[^3H]Phorbol$ 12,13-dibutyrate (PDBu) bound to PKC was measured in the presence of test materials. Total methanol extracts from 100 kinds of natural products were partitioned into 3 fractions (n-hexane, ethyl acetate and aqueous layer) and their binding ability to the regulatory domain of PKC was evaluated. The ethyl acetate fractions of Morus alba $(roots,\;IC_{50}:\;156.6\;{\mu}g/ml)$, Rehmannia glutinosa $(roots,\;IC_{50}:\;134.3\;{\mu}g/ml)$, Lysimachia foenum-graecum $(roots,\;IC_{50}:\;167.8\;{\mu}g/ml)$, Polygonum cuspidata $(roots,\;IC_{50}:\;157.3\;{\mu}g/ml)$, Cnidium officinale $(aerial\;parts,\;IC_{50}:\;145.2\;{\mu}g/ml)$, and the hexane $(IC_{50}:\;179.3\;{\mu}g/ml)$ and the EtOAc fraction of Symplocarpus nipponicus $(roots,\;IC_{50}:\;155.9\;{\mu}g/ml)$ showed inhibitory activity of $[^3H]PDBu$ binding to PKC.

• 대한수의학회지
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• 제56권2호
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• pp.97-101
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• 2016

Emodin is an anthraquinone derivative from the roots of Rheum officinale Baill that possesses a variety of biological activities, including inhibition of $5{\alpha}$-reductase and prostaglandin D2. In this study, we investigated whether emodin promotes hair growth. After emodin was topically applied to the shaved dorsal skin of telogenic C57BL/6 N mice, the hair growth rate and morphological analysis were evaluated in dorsal skin for 15 days. After 13 days of treatment, minoxidil or emodin (0.01% or 0.1%)-treated groups showed remarkable regrowth of hairs relative to the vehicle control group. Scoring of the hair growth and rate of hair growth area for 15 days revealed that groups treated with minoxidil and 0.1% emodin were significantly higher than the vehicle control group. Histological examination revealed the emodin and minoxidil groups markedly recovered the number and morphology of hair follicles, including the subcutis depth, relative to the vehicle group. These results suggest that emodin has an excellent promoting effect in hair growth similar to that of minoxidil and might be useful for treatment of baldness or alopecia.

• 한국약용작물학회지
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• 제15권1호
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• pp.26-29
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• 2007

장내미생물 개선에 효과가 있는 약용식물소재들을 탐색하여 정장제품 개발하고자 13종의 식물을 물과 에탄올로 각각 추출하여 농도별로 유해균인 C. perfringens, E. coli와 유익균인 B. longum의 생육에 미치는 영향을 in vitro실험으로 시험한 결과는 아래와 같다. 가. C. perfringens은 민들레와 인진쑥 물 추출물에, 돌나물, 오미자, 민들레, 부추, 생강, 쑥, 인진쑥, 질경이 에탄올추출물에 강하게 생육억제 되었다. 나. E. coli은 돌나물, 오미자 물과 에탄올 추출물에서 강하게 생육억제 되었다. 다. B. longum은 추출용매에 관계없이 생육에는 크게 영향을 주지 않았다. 라. 돌나물과 오미자 에탄올추출물이 유익균에 생육억제 없이, 두 유해균을 억제됨으로서 이들 소재가 정장제품으로의 개발 가능성이 있다고 생각되었다.

• 한국식품영양과학회지
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• 제23권3호
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• pp.466-471
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• 1994

The antioxidant activity of methanol extracts of thirty plants was tested using the methol of 1, 1-diphenyl-2-pi-cryl hydrazyl (DPPH) reactivity. Four methanol extracts from Zingiber officinale, Piper nigrum , Zanthoxylum schinifolium and Capsocum annuum were found to be the most effective on DPPH radical scavenging activity. The next effective ones were Perilla frutescens , Sedium sarmentosum , Raphnus sativas, aArctium lappa, Beta vulgaris. Brassica oleracea var. Acephala, bBrassica juncea inorder, and the others did not show a considerable activity. The methanol extract obtained from the seed coats of Zanthoxylum schinifolium was fractinated with several sovlents. The interphase materials exhibited the strongest antioxidant activity and was further purified by silica gel and Sephadex LH-20 column chormatography. Two active principles were isolated and identified as quercetin -3-O-$\alpha$-L-rhamonopyranoiside(quercitrin) and quercetin 3-O-$\alpha$-D-galactopyranoside (hyperoside) by ultraviolet(UV), proton nuclear magetic resonance (1H-NMR) and carbon nuclear magnetic resonance (13C-NMR). Its antioxidative activity was a little higher that that of L-ascorbic acid.

• Applied Biological Chemistry
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• 제41권8호
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• pp.605-607
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• 1998

A total of 28 traditional drinks derived from 23 plant species in 19 families were tested for their in vitro growth-inhibiting effects against Bifidobacterium adolescents, Bifidobacterium bifidum, Lactobacillus acidophilus, Lactobacillus casei, Clostridium perfringens, and Escherichia coil using a paper disc agar diffusion method under anaerobic condition. The responses varied with bacterial strain, plant species and tissue sampled. In a test with C. perfringens at 5 and 10 mg/disc, potent growth inhibition was produced from the extracts of Eucommia ulmoides stems, Pinus densiflora leaves and shoots, Thea sinensis leaves (green and oolong teas) and Zingiber officinale roots. All materials tested did not adversely affect the growth of bifidobacteria, lactobacilli, and E. coli. These results may be an indication of at least one of the pharmacological activities of these plant-derived drinks.

• 한국식품조리과학회지
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• 제27권2호
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• pp.31-43
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• 2011

The purpose of this study was to develop Zingiber officinale Roscoe powder(ginger powder) sweet rice muffins. The process included substituting sweet rice flour for cake flour and adding ginger powder. The mixing conditions for the ginger powder sweet rice muffins were a control and three levels of ginger powder (A), whole egg (B), and soybean oil (C) using central composite design, and were optimized by response surface methodology. The optimization was analyzed with regard to physical, textural, and sensory properties. Among the sensory properties, variables that appeared to show significant values, such as appearance, flavor, softness and overall quality were used to identify optimums. The numerical and graphical methods used in this study determined that the optimum formulation for ginger powder sweet rice muffins was 8.39 g of ginger powder, 215.37 g of whole egg, and 54.40 g of soybean oil. Ginger increases appetite, enhances metabolism and reduces the speed of aging. These health advantages of ginger provide evidences that ginger powder sweet rice muffins are a good healthy snack, particularly for the elderly.