• 한국자원식물학회지
• /
• 제18권2호
• /
• pp.240-245
• /
• 2005

더덕 뿌리에서 유래한 EST cDNA library로부터 GST(glutatione S-transferase)유전자와 높은 상동성을 나타내는 full clone cDNA를 얻었다. 더덕의 GST(glutatione S-transferase), CIGST은 761 bp의 cDNA로 173개의 아미노산을 코딩하는 522 bp의 ORF를 가지고 있으며, A. thaliana(AAC63629) $71\%$, C. chinense(CAI51314) $73\%$, E. esula(AAE65767) $75\%$, H. muticus(CAA55039) $70\%$, N. plumbaginifolia(CAA96431) $77\%$, S. commersonii(AAB65163) $76\%$등 다른 식에서 밝혀져 있는 GST(glutatione S-transferase)와 유의한 상동성을 나타내였다.

• 한국약용작물학회지
• /
• 제22권2호
• /
• pp.134-139
• /
• 2014

This study was to investigate the improvement of immune activities of the extracts from Codonopsis lanceolata by stepwise steaming process and high pressure process. The phenol contents was $8.742{\mu}g/mg$ which was higher than that from conventional extraction using 70% ethyl alcohol at $80^{\circ}C$ for 24 hours. All of extracts at a concentration of $1.0mg/m{\ell}$ showed relatively low cytotoxicity on human normal kidney cell (HEK293) in range of 16 19%. The immune B and T cell growth was improved by extracts using the steamed and high pressure precess of C. lanceolata up to $180{\times}10^4cells/m{\ell}$ and $96{\times}10^4cells/m{\ell}$, respectively. The extract prepared also greatly increased the secretion of both IL-6 and TNF-${\alpha}$ from the stepwise steamed and high pressure process. This results can conclude that stepwise steamed and high pressure process effectively released active biomaterials which could important role in enhancing immune activity in the body.

• 한국식품조리과학회:학술대회논문집
• /
• 한국식품조리과학회 2003년도 추계학술대회 및 사단법인 창립총회
• /
• pp.74-74
• /
• 2003

We investigated the microbial populations and viable cell counts of Codonopsis lanceolata from uncultivated and cultivated soil in the suing. The microbial populations and viable cell counts from both types of soils were also investigated simultaneously. It had existed 10 more kinds of microorganisms in uncultivated than those in cultivated. The total viable cell counts of C. laneolata from uncultivated soil, especially in the upper zone, were 9.7$\times$10$^{6}$ CFU/g. However, the C. lanceolata from cultivated soil was 4.2$\times$10$^{6}$ CFU/g. (omitted)

• Plant Resources
• /
• 제8권3호
• /
• pp.181-187
• /
• 2005

A cinnamoyl CoA reductase (CCR) cDNA (ClCCR) was isolated from tabroot mRNAs of Codonopsis lanceolata by cDNA library construction, and its expression was investigated in relation to abiotic stresses. The ClCCR is 1008 bp in length with an open reading frame (ORF) of 336 amino acids. The deduced amino acid sequence was showed high similarity with cinnamoyl-CoA reductases of P. tremuloides (AAF43141) 87%, F.${\times}$aranassa (AAP46143) 83%, L. album (CAD29427) 80%, E. gunnii (CAA66063) 72%, S. tuberosum (AAN71761) 83%. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis was revealed that the ClCCR expression was regulated by abiotic stresses.

• 식물분류학회지
• /
• 제32권3호
• /
• pp.301-338
• /
• 2002

한국산 사초속 사초아속(Eucarex Cross & Germ.) 피사초절(section Rhomboidales Nees) 4종과 그늘사초절(section Digitatae Fr.) 7종을 대상으로 외부형태학적 형질을 재검토하고, 광학현미경과 주사전자현미경을 사용하여 과낭, 수과와 잎의 표피형을 비교 분석하였다. 그 결과 얻어진 정량적 형질(줄기, 잎, 이삭, 비늘조각, 포, 과낭과 수과의 길이와 너비, 포의 수)과 정성적 형질(이삭, 비늘조각, 과낭과 수과의 모양, 엽설의 유무, 줄기단면과 비늘조각 윗부분의 모양) 및 과낭, 수과와 잎의 표피형(기본표피세포모양, 세포벽의 굴곡, 규소체의 수와 모양, 기공복합체의 크기와 빈도, 부세포의 모양)이 종을 동정, 식별하는데 유용하였다. 본 연구 결과 잎, 줄기, 암꽃 비늘조각과 과낭의 길이, 과낭부리 모양과 부리 표피형이 피사초절과 그늘사초절을 나누는 유용한 형질이 되었다. 그리고 외부형태학적 특징이 유사하여 동정에 어려움이 있어온 가는잎그늘사초(C. humilis Leyss.)와 그늘사초(C. lanceolata Boott.)는 줄기의 길이, 엽설의 유무, 줄기 단면모양과 과낭, 수과와 잎의 표피형에서 차이를 보여 두 종을 식별할 수 있었다. 또한 넓은잎그늘사초(C. pediformis C. A. Meyer)를 그늘사초(C. lanceolata Boott)의 아종이나 변종으로 취급하는 등 학명사용에 혼란이 있어 왔으나 과낭과 수과의 모양, 줄기 단면의 모양과 과낭, 수과와 잎의 표피형에서 뚜렷히 구분되어 각각 다른 종으로 취급하였고 학명과 국명을 정리할 수 있었다.

• 한국약용작물학회지
• /
• 제20권6호
• /
• pp.493-499
• /
• 2012

We have established adventitious root culture systems of Codonopsis lanceolata, Codonopsis pilosula and Codonopsis ussuriensis. Root segments of C. lanceolata were the best explants for induction of adventitious roots and the number of adventitious root for explant was highest on solid medium with $0.5mg/{\ell}$ NAA and produced $18.8{\pm}1.9$ roots per explant. Root segments of C. pilosula were the best explants for induction of adventitious roots and the number of adventitious root for explant was highest on solid medium with $1.0mg/{\ell}$ NAA and produced $8.5{\pm}1.8$ roots per explant. Leaf segments of C. ussuriensis were the best explants for induction of adventitious roots and the number of adventitious root for explant was highest on solid medium with $0.5mg/{\ell}$ NAA and produced $7.8{\pm}0.4$ roots per explant. In liquid culture, the best production of adventitious root (fresh weight) was obtained in 1/2 MS medium with $1.0mg/{\ell}$ NAA. This study demonstrated for the first time to produce adventitious roots in C. pilosula and C. ussuriensis.

• 한국자원식물학회지
• /
• 제5권1호
• /
• pp.1-10
• /
• 1992

Germination characteristic between Korean species and Japanese one were identified. Seed waslow temperture germinability. Optimum temperature of germination:on was 16$^{\circ}C$ -19$^{\circ}C$. Germinationrate was effected more when treated with GA3 of 1000ppm at shade and light place. Germinationrate was no significant difference between Korean species and japanese one. e.

• 생약학회지
• /
• 제25권3호
• /
• pp.204-208
• /
• 1994

For the investigation of resources in Codonopsis species, the studies were carried out to evaluate the pharmaco-constituents from the leaves of Codonopsis lanceolata (Campanulaceae) whose roots have been used to antitussive, expectorant, detoxicate, tonic, edible, etc. as a folk medicine in Korea. From the EtOAc and BuOH fractions of MeOH extract, three flavonoid compounds, $luteolin-7-O-{\beta}-_D-glucopyranoside$$(C_{21}H_{20}O_{1i},\;mp\;254{\sim}255^{\circ}$, compound 1), $luteolin-5-O-{\beta}-_D-glucopyranoside$$(C_{21}H_{20}O_{1i},\;mp\;279{\sim}281^{\circ}$, compound 2) and luteolin $(C_{15}H_{10}O_6,\;mp\;327{\sim}330^{\circ}$, compound 3) were isolated and identified on the basis of their physicochemical properties and spectroscopic evidences(UV, IR, $^1H-NMR$, $^{13}C-NMR$, MS etc.) in comparison with authentics respectively.

• 한국식품과학회지
• /
• 제48권2호
• /
• pp.104-110
• /
• 2016

본 연구에서는 더덕 껍질의 활용도를 높이고 식품소재, 특히 차 원료로서의 가능성을 제시하기 위하여 더덕을 껍질과 육질로 나누어 가열 처리한 더덕으로부터 열수추출물을 제조하여 이화학 및 관능 특성을 비교하였다. 더덕 열수추출물의 pH는 껍질이 육질보다 낮았고(p<0.05), 색도는 볶은 껍질과 육질의 L값이 낮고 b값이 높았다(p<0.05). 총당 함량은 껍질의 경우에 증건군이 볶음군보다 많았으나(p<0.05), 환원당 함량은 껍질의 경우에 열풍건조군이, 육질의 경우에 대조군이 많았다. 조 사포닌 함량은 대조군 육질, 볶은 육질, 열풍건조한 육질에 많았다(p>0.05). 껍질의 열수추출물이 육질의 열수추출물보다 총 폴리페놀 함량이 많고 DPPH 라디칼 소거능도 높았다(p<0.05). 더덕 분말을 차로 만들어 관능검사한 결과, 더덕 껍질로 만든 차는 향과 전반적 기호도에서 더덕 육질로 만든 차와 유의적 차이가 없었다(p>0.05). 결론적으로 대부분 폐기물 처리되는 더덕 껍질은 육질보다 총 폴리페놀 함량이 많고 산화방지 활성이 뛰어났으며, 더덕 껍질 차의 향과 전반적 기호도가 더덕 육질로 만든 차와 유사한 관능 특성을 가지고 있으므로 차의 재료로 활용할 수 있을 것으로 기대한다.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.273-273
• /
• 2017

Polyploidy has opened a new horizon for selection to sculpt a variety of new gene functions, traits, and lineages. The aim of this study was to investigate the efficacy of the colchicine concentration, temporal changes, and suitable material for inducing effective tetraploid plants of Codonopsis lanceolata. A total of 180 individuals from 16 treatment groups were germinated, and exposed to different concentrations of Colchicine. The plant height of the diploid (18.1 cm) was slightly shorter than that of the tetraploid (13.4 cm). The fresh weight of the main root in the diploid (0.5 g) was 4-fold higher than the tetraploid (2.2 g). The colchicine-treated plant regeneration rate in C. lanceolata was decreased at the elevated concentration of colchicine. A total of 126 individual plants were regenerated in the entire treatment group and tetraploid (2n=4x=32) plants were obtained. In particular, 5 individuals of the tetraploid plant were induced in the 0.05% colchicine for 6h, which is a higher rate (29.4%) than other regenerated plants. As in the seed treatment result, the plant height of the diploid was significantly higher (10.4 cm) than tetraploid. The root length of the tetraploid (10.1 cm) was longer than the diploid, and the root was also thicker. Taken together, the results obtained from the present study may be helpful for the efficient recovery of such polyploid plants through the in vitro application of colchicine, and may improve the productivity and breeding of C. lanceolata.