• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.82-82
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• 2018

본 연구는 국내에 자생하는 자원식물들의 살초활성을 조사하여 식물생장억제물질을 활용한 환경친화형 제초제 개발에 요구되는 기초 자료를 확보하기 위해 수행되었다. 국내 자생 식물 101종을 식물체 부위별로 나누어 MeOH로 추출하여 획득된 시료 129점에 대해 돌피(Echinocholoa crus-galli P.B. var. formosensis Ohwi)를 이용한 살초활성 검정을 실시하였다. 시료를 소량의 methanol로 녹여 직경 5.5cm petri dish 상의 Whatman No. 2 여지에 균일하게 흡착시킨 후, fume hood 내에서 용매를 제거하고 1mL의 증류수를 첨가하였다. 돌피 종자를 15립씩 3반복으로 파종하여 $28^{\circ}C$, 5,000lux의 growth chamber에 치상하고, 7일 후 시험구 당 균일하게 자란 10개의 유묘를 선발해 초장 및 근장을 조사하였으며, 살초활성 평가를 위해 대조구 개체의 초장과 근장에 대한 생장억제율을 산출하였다. $1,000{\mu}g/mL$ 농도에서 101종 129점 MeOH 추출물들의 돌피 유묘 뿌리 생장에 대한 억제율을 조사한 결과, 80% 이상을 나타낸 식물 시료는 가죽나무 줄기, 누린내풀 지상부, 단풍취 뿌리, 두릅나무 지상부, 백양꽃 전초, 백양꽃 지하부, 병조희풀 경엽부, 산사나무 잎 가지, 삽주 뿌리, 상사화 전초, 상사화 지하부, 석산 뿌리, 애기송이풀 전초 등 17점이었다. 이들 중 90% 이상 고활성을 나타낸 단풍취 뿌리, 두릅나무 지상부, 백양꽃 전초, 상사화 지하부, 석산 뿌리 유래의 추출물에는 새로운 환경친화형 제초제 개발소재로써 활용할 수 있는 살초활성물질들이 함유되어 있는 것으로 생각된다.

• Korean Journal of Weed Science
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• v.9 no.2
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• pp.116-122
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• 1989

An experiment was carried out at the International Rice Research Institute in 1987 to understand the seed dormancy and germination habit of rice and several weed species. The germinability of the weed seeds just after harvest was variable depending on the species and ranged from 0 to 72%. Two rice cultivars, IR64(lowland type) and UPLRi-5(upland type) had higher than 95% in germination ability throughout the experimental period due to the fact that the rice seeds came from the harvest of the previous season and dormancy had already been overcome. The length of the storage period needed to overcome dormancy at room temperature($25{\pm}2^{\circ}C$) was about 50 days for Echinochloa glabrescens Munro ex Hook, f., more than 60 days for E. crus-galli ssp. hispidula (Retz.) Honda and 20 days for Ludwigia octovalvis(Jacq.) Raven. Seeds of E. colona(L.) Link, Monochoria vaginalis(Burm. f.) Presl, Fimbristylis miliacea(L.) Vahl and Cyperus difformis L. appeared to have no dormancy. Among the nine species M. vaginalis had the lowest germination of less than 1% throughout the experimental period. However, its seed germinated easily when planted in soil. The low germinability of E. glabrescens, E. crus-galli ssp. hispidula and L. octovalvis just after harvest could be overcome through pretreatment of seeds either by soaking in nitric acid(0.1N) for 1 day or removal of the hull in the grass species, the nitric acid treatment being superior. The results imply that germination habit of weed species varied depending on the species through their differential dormancy period or differential germination strategy.

• The Korean Journal of Pesticide Science
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• v.5 no.3
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• pp.12-17
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• 2001

A new 2,3-dihydro-2,3,4,6,7-pentamethylbenzofuran-5-yl substituents in 5-benzofuryl-2-[1-(alkoxyimine) alkyl]-3-hydroxycyclohex-2-en-1-ones were found to have herbicidal activity against rice plant (Oryza sativa L.) and barnyard grass (Echinochloa crus-galli) under submerged conditions. The structure activity. relationships (SARs) on the herbicidal activity of $R_1\;and\;R_2$ on the azomethine bond of 2,3-dihydro-2,3,4,6,7-pentamethylbenzofuran-5-yl subsituents were analysized. The conditions of the selective herbicide activity between rice plant (seed) and barnyard grass from the basis on the analysized SARs are assumed that the $R_2$ group of ethyl substituent ($R_1$ ) should have optimal asymmetry parameter, $(L/B_1)_{opt.}=3.96{\AA}$. And also, the $R_2$ groups consist of $C_1{\sim}C_3$ and unsaturated group such as 3-chloro-2-propenyl group was contribution to the herbicide activity.

• Korean Journal of Weed Science
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• v.30 no.3
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• pp.183-190
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• 2010

The objective of this study was to isolate a herbicidally active compound from curly dock (Rumex crispus), a native weed in Korea and to identify its' chemistry. The $GR_{50}$ value of methanol extracts which is determined by a seed bioassay using rapeseed (Brassica napus) seedlings was $935\;{\mu}g\;g^{-1}$. Activity-directed isolation of ethylacetate extract led to the isolation of ECDA fraction with $GR_{50}$ value $53\;{\mu}g\;g^{-1}$. Based on data of GC-MS, $^1H$-NMR and $^{13}C$-NMR, the chemical structure of ECDA was determined as 2H-furo[2,3-H]-[1]-benzopyran-2-one which is known as angelicin. The $GR_{50}$ values of angelicin to barnyardgrass (Echinochloa crus-galli), southern crabgrass (Digitaria ciliaris), and indian jointvetch (Aeschynomene indica) were 426, 66 and $216\;{\mu}g\;g^{-1}$, respectively. Our results suggest that angelicin could be used as a lead compound for the development of new herbicides.

• Korean Journal of Weed Science
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• v.18 no.2
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• pp.116-121
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• 1998

Field experiment were carried out to elucidate the changes of weed occurrence according to the sowing time and to establish the efficient weed control system in dry seeded rice cultivation. E crus-galli and D. sanguinalis are tow major weeds at the earlier sowings, but C. difformis was dominating at the later sowing. In the case of E. crus-galli, there was a tendency of gaining its dominance values as the rice growth stage advances. The later the sowing time was, the more weeds occurred at the early rice growth stages: However, when measured at heading, total dry weight was greater at the earlier sowings. Yield loss due to weeds were also greater at the earlier sowings. Community diversity of weeds was greater at the later sowings, while specific weed was gradually dominating as rice growth stage advances, regardless of sowing time. When herbicides were applied before 15 DAS, weeds were effectively controlled only upto 35 DAS. So systemic herbicide application, one on 0 to 10 DAS and the other on just after flooding, appeared to be recommendable in direct seeded rice cultivation.

• Korean Journal of Weed Science
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• v.8 no.3
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• pp.273-282
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• 1988

The seventeen barnyardgrass [Echinochloa crus-galli (L.) Beauv.] accessions, which were collected national-widely in 1986 and selected two times through 1987, were experimented at 1988. To study the degree of ecological variations of the collected accessions of native barngardgrass species as a weed, the experiment was conducted with Wagner pots in size of 1/5000a and under P.E. film house. Among 57 characters, 100 grains weight, rate of tiller increase from transplanting to 30 days, the number of the maximum tillers and rate of dry weight increase from 15 DAS to 22 DAS were become intra-specific variarions as higher coefficient of varation than 30%, the size of the first leaf, fresh weight and dry 22 seedlings, rate of productive tiller, panicle number and tillering divergence made intra-specific variations as more and less C. V. than 25%, while the required days upto heading since seeding at June 6. rate of fresh/dry weight and plant height of 22 days old seedling made intra-specific variations as the coefficient of variation of about 50%. Among the attributes the color in leaf, leaf sheath, mid-ribs, node, spike, grain, and plant type, length of leaf marginal tooth, grain shape, purple bend on leaf were seemed useful to indentify the accessible specific.

• Korean Journal of Weed Science
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• v.7 no.2
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• pp.208-219
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• 1987

Newly developed several herbicides were evaluated as paddy rice herbicide at the Yeongnam Crop Experiment in 1986. And also, the general situation of rice cultivation between Korea and Japan was compared. Twenty-nine herbicides of the total 59 herbicides were used as paddy rice field in Korea while these were 100 and 187, respectively, in Japan. Among paddy rice herbicides, butachlor was the most important herbicide in both countries. However, the degree of concentration to a particular herbicide was greater in Korea compared to Japan; consumption rate of single butachlor to the total herbicide were 66.5% for Korea and 11.9%r for Japan, respectively. Pyrazolate, pyrazoxyfen and quinclorac were the most promising hebicides in pressed-type rice nurserybed in terms of herbicidal efficacy and phytotoxic effect. For transplanted paddy rice field, single application of NC-311 or combining applications of NC-311 with butachlor or quinclorac gave excellent results at the weed community that was dominated by Echinochloa crus-galli, Aneilema japonica, Ludwigia prostrata, Scirpus hotarui, Cyperus serotinus, Potamogeton distinctus and Eleocharis kuroguwai. Particularly the above combining applications maintained their excellent herbicidal effect up to 3 leaf stage of E. crus-galli without arising herbicidal phytotoxicity. Pyrazolate and three sulfonyl urea herbicides (DPX-5384, NC-311 and CGA 142464) exhibited very high safety against rice. However, Japonica type rice cultivar was a little bit more sensitive than Indica/Japonica type rice cultivar. On the other hand, the effect of these herbicides against E. crus-galli was very strong. Herbicidal effect against E. crus-galli was enhanced through shoot absorption for sulfonyl urea herbicides and root abosorption for pyrazolate and quinclorac, respectively.

• The Korean Journal of Ecology
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• v.23 no.6
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• pp.431-434
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• 2000

To examine the allelopathic potentials of Chrysanthemum boreale, aqueous extracts and essential oil of the plant were used in these experiments. Seed germination of the receptor species was inhibited by the aqueous extracts and the inhibitory effect was increased in proportion to the concentration of extracts. In contrast, seedling elongation showed varied results. Achyranthes japonica, Bidens bipinnata, Raphanus sativus var. hortensis for. acanthiformis, Plantago asiatica, Pimpinella brachycarpa and Lactuca sativa were inhibited by increasing concentration of the aqueous extract, while Brassica campestris subsp. napus var. pekinensis and Echinochloa crus-galli were stimulated by the extract. Dry weight was also inhibited proportionally by increasing concentration of the aqueous extract, while some species were stimulated by a lower concentration of the extract. The volatile substances of C. boreale did not affect the seed germination of receptor plants, but seedling elongation and dry weight of some species were inhibited dose-dependently. Root hair development of selected plants was inhibited along with the concentration of essential oil. The above mentioned results, therefore, confirmed that the natural substances from C. boreale had allelopathic potentials to other plants.

• The Korean Journal of Ecology
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• v.19 no.5
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• pp.431-451
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• 1996

The present studwas undertaken to classify and describe the riverside vegetation around Hanchon (36°27’-36°51’N, 128°15’-128°37’E), a tributary in the upper stream area of Nak-tong River, South Korea by methods of the ZM school of phytosociology. As a result the vegetation was divided into twenty three associations and communities belonging to ten classes. Reflecting various human impacts in the past, the associations and communities of the Artemisietea principis were most plentiful there. The vegetation units obtained in the present study were as follows: A. forest vegetation A-1 Fagetea crenatae:Zelkova serrata community, Larix leptolepis community; B. Shrub vegetation B-1 Salicetea sachalinensis:Salicetum gracilistylae, Salicetum graciliglandis assoc. nov., B-2 Rosetea sachalinensis:Salicetum gracilistylae, Salicetum graciliglandis assoc. nov., B-2 Rosetea multiflorae:Stephanandra incisa community, Lycium chinense community; C. Grassland vegetation C-1 Lemnetea minoris:Spirodela polyrhiza-Lemna paucicostata community, C-2 Bidentetea tripartiti:Polygonetum thunbergii, Polygonetum sieboldii-hydropiperis, Panico Polygonetum hydropiperis, Pharagmitetea :Oenantho- Phalaridetum arundinaceae, Phragmitetum japonicae, Miscanthetum sacchariflori, Phragmites australis community, C-4 Artemisietea principis:Artemisia princeps community, Impatiens textori community, Lactuco indicae-Humuletum japonicae, Pueraria lobata-Humulus japonicus community, Pennisetum alopecuroides community, C-5 chenopodietea:Echinochloa crus-galli var. orizicola community, Digitaria adsecendens community, Polygonum nodosum community, C-6 Miscanthetea sinensis: Miscanthus sinensis community, C-7 Plantaginetea:Eragrotis multicaulis-Plantago asiatica community. It is inferred that the endemic vegetation units in the riverside vegetation of Korea are very rare, because most of the vegetation units obtained in the present study are common with those of Japan. Among the above vegetation units, the Salicetum graciliglandis was established as a new association. On the other hand, the associations and communities of classes characterizing the riverside vegetation increased with a decrease of human impacts from the downstream to the upstream in Hanchon, while the number of the naturalized plants was the reverse. Also based on the present phytosociological work, the relation between the vegetation units and their environmental conditions and the restoration ecology and nature conservation of the riverside vegetation were discussed here in detail.

• Korean Journal of Weed Science
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• v.11 no.3
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• pp.224-228
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• 1991

This study was carried to know factors affecting emergence of major upland weeds in soil in order to get basic information on weed control methods. Firthy eight percent of weed seeds were distributed within soil surface to 10cm in soil and 2% of weed seeds were observed in 40~50cm soil layer in field. As planting depth was deeper, emergence of weeds became poor. However Capsella bursa-pastoris can emerge at soil surface. Amaranthus retroflexus, Amaranthus lividus, Porturaca oleracea, Chenopodium album, Solanum nigrum upto 3cm, Eleusine indica, Echinochloa crus-galli, Setaria viridis, Digitaria sanguinalis upto 7cm. Emergence of weeds was very poor in very acid soil but good in soil pH 5.5~6.0. However emergence of weed was not affected by pH 3.5~4.0 or above. Emergence of E. indica, C. bursa-pastoris, A. retroflexus, A. lividus, C. album, E. crus-galli, Solanum nigrum, and S. viridis was good in loam soil and P. oleranea, D. sanguinalis in sandy loam soil. Emergence of weed seeds was not affected by fertilization.