• Journal of Korean Medicine for Obesity Research
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• v.20 no.1
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• pp.10-19
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• 2020

Objectives: Oxidative stress-mediated neuroinflammation has been supposed as a crucial factor that contributes to the pathogenesis of many neurodegenerative diseases. In this study, we aimed to investigate the protective activity against oxidative stress and neuroinflammation of protocatechuic acid (PA), active phenolic compound from Momordica Charantia. Methods: Protective activity of PA from oxidative stress was performed under in vitro conditions. Our study investigated the protective mechanism of PA from neuroinflammation in cellular system using C6 glial cell. To investigate the improvement the effects on oxidative stress and neuroinflammation, we induced oxidative stress by H₂O₂ (100 μM) stimulation and induced neuroinflammation by treatment with lipopolysaccharide (LPS) (1 ㎍/mL) and interferon-gamma (IFN-γ) (10 ng/mL) in C6 glial cells. Results: PA showed strong radical scavenging effect against 1,1-dipenyl-2-picrylhydrazyl, hydroxy radical (·OH) and nitric oxide (NO). Under oxidative stress treated by H₂O₂, the result showed the increased mRNA expressions of oxidative stress markers such as nuclear factor-kappaB (NF-κB), cyclooxygenase (COX-2) and inducible nitric oxide (iNOS). However, the treatment of PA led to reduced mRNA expressions of NF-κB, COX-2 and iNOS. Moreover, PA attenuated the production of interleukin-6 and scavenged NO generated by both endotoxin LPS and IFN-γ together. Furthermore, it also reduced LPS and IFN-γ-induced mRNA expressions of iNOS and COX-2. Conclusions: In conclusion, our results collectively suggest that PA, phenolic compound of Momordica Charantia, could be a safe anti-oxidant and a promising anti-neuroinflammatory molecule for neurodegenerative diseases.

• Journal of Ecology and Environment
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• v.41 no.6
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• pp.165-172
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• 2017

Background: This study investigated the temporal variation in soil $CO_2$ efflux and its relationship with soil temperature and precipitation in the Quercus glauca and Abies koreana forests in Jeju Island, South Korea, from August 2010 to December 2012. Q. glauca and A. koreana forests are typical vegetation of warm-temperate evergreen forest zone and sub-alpine coniferous forest zone, respectively, in Jeju island. Results: The mean soil $CO_2$ efflux of Q. glauca forest was $0.7g\;CO_2\;m^{-2}\;h^{-1}$ at $14.3^{\circ}C$ and that of A. koreana forest was $0.4g\;CO_2\;m^{-2}\;h^{-1}$ at $6.8^{\circ}C$. The cumulative annual soil $CO_2$ efflux of Q. glauca and A. koreana forests was 54.2 and $34.2t\;CO_2\;ha^{-1}$, respectively. Total accumulated soil carbon efflux in Q. glauca and A. koreana forests was 29.5 and $18.7t\;C\;ha^{-1}$ for 2 years, respectively. The relationship between soil $CO_2$ efflux and soil temperate at 10 cm depth was highly significant in the Q. glauca ($r^2=0.853$) and A. koreana forests ($r^2=0.842$). Soil temperature was the main controlling factor over $CO_2$ efflux during most of the study period. Also, precipitation may affect soil $CO_2$ efflux that appeared to be an important factor controlling the efflux rate. Conclusions: Soil $CO_2$ efflux was affected by soil temperature as the dominant control and moisture as the limiting factor. The difference of soil $CO_2$ efflux between of Q. glauca and A. koreana forests was induced by soil temperature to altitude and regional precipitation.

• Journal of Environmental Science International
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• v.17 no.12
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• pp.1381-1390
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• 2008

Emission characteristics of volatile organic compounds (VOCs) were investigated in the flue gas emitted from wood drying process for plywood manufacturing. The moisture content of raw timber was average 48%, and its density was $831.55kg/m^3$. But the moisture content of dried wood is needed less than around 10%, thus the moisture contents of flue gas should be remarkably high(about 18.2 V/V%). Therefore, the vapor in flue gas is equivalent to 320 ton-vapor/day when 1100 ton-wood/day is treated in the wood drying process. The temperature of flue gas ranges from $140^{\circ}C\;to\;150^{\circ}C$ in each dryer stack with exception of the input site of wood(about $110^{\circ}C$). The velocity of flue gas in each stack ranges from 1.7 to 9.7m/sec. In order to assess the concentrations and attribution rate of odorous compounds, it was analyzed about 40 VOCs in the flue gases. It was found that the major odorous compounds were 8 compounds, and the concentrations of major VOCs(ppm) were as follows; benzene: $0.054{\sim}0.052$, toluene: $1.011{\sim}2.547$, ethylbenzene: $0.472{\sim}2.023$, m,p-xylene: $0.504{\sim}3.245$, styrene: $0.015{\sim}0.148$, o-xylene : $0.271{\sim}1.097$, ethanol: $11.2{\sim}32.5$, ${\alpha}$-pinene: $0.908{\sim}10.578$, ${\beta}$-pinene: $0.982{\sim}14.278$. The attribution rate of terpenes (${\alpha}$-pinene, ${\beta}$-pinene) was about 60.56%, and that of aromatics and alcohols was about 22.77%, and 16.67%, respectively. It is suggested that the adequate control device should be used to control both the water soluble and non-soluble compounds because both compounds were mixed in flue gas.

• Genomics & Informatics
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• v.11 no.4
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• pp.263-271
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• 2013

We investigated the contribution of genetic variations of KLF5 to basal metabolic rate (BMR) and resting metabolic rate (RMR) and the inhibition of obesity in Korean children. A variation of KLF5 (rs3782933) was genotyped in 62 Korean children. Using multiple linear regression analysis, we developed a model to predict BMR in children. We divided them into several groups; normal versus overweight by body mass index (BMI) and low BMR versus high BMR by BMR. There were no differences in the distributions of alleles and genotypes between each group. The genetic variation of KLF5 gene showed a significant correlation with several clinical factors, such as BMR, muscle, low-density lipoprotein cholesterol, and insulin. Children with the TT had significantly higher BMR than those with CC (p=0.030). The highest muscle was observed in the children with TT compared with CC (p=0.032). The insulin and C-peptide values were higher in children with TT than those with CC (p=0.029 vs. p=0.004, respectively). In linear regression analysis, BMI and muscle mass were correlated with BMR, whereas insulin and C-peptide were not associated with BMR. In the high-BMR group, we observed that higher muscle, fat mass, and C-peptide affect the increase of BMR in children with TT (p < 0.001, p < 0.001, and p=0.018, respectively), while Rohrer's index could explain the usual decrease in BMR (adjust $r^2$=1.000, p < 0.001, respectively). We identified a novel association between TT of KLF5 rs3782933 and BMR in Korean children. We could make better use of the variation within KLF5 in a future clinical intervention study of obesity.

• Bulletin of the Korean Chemical Society
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• v.8 no.2
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• pp.96-101
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• 1987

2-Ethoxy-6-methoxy-5-cyano-3,4-dihydro-2H-pyran (1_a$), 2-n-butoxy-6-methoxy-5-cyano-3,4-dihydro-2H-pyr an (1b), 2-isobutoxy-6-methoxy-5-cyano-3,4-dihydro-2H-py ran ($1_c$), and 2-ethoxy-6-methoxy-3-methyl-5-cyano-3,4-dihydro -2H-pyran ($1_d$) were prepared by (4 + 2) cycloaddition reaction of methyl $\alpha$-cyanoacrylate with the corresponding alkyl vinyl ethers. Compounds $1_{a-d}$ were ring-open polymerized by cationic catalyst to obtain alternating head-to-head (H-H) copolymers. For comparison, head-to-tail (H-T) copolymer $3_a$ was also prepared by free radical copolymerization of the corresponding monomers. The H-H copolymer exhibited minor differences in its $1_H% NMR and IR spectra, but in the $^{13}C$ NMR spectra significant differences were observed between the H-H and H-T copolymers. Glass transition temperature ($T_g$) of H-H copolymer was higher than that of the H-T copolymer, but thermal decomposition temperature of the H-H copolymer was lower than that of the H-T copolymer. Compounds $1_a$, $a_b$, and $1_c$, copolymerized well with styrene by cationic catalyst, but compound 1d failed to copolymerize with styrene. All of the H-H and H-T copolymers were soluble in common solvents and the inherent viscosities were in the range 0.2-0.4 dl/g.

• Korean Journal of Applied Biomechanics
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• v.31 no.3
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• pp.205-213
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• 2021

Objective: The aim of this study was to analyze body stability Joint coordination pattern though as bending stiffness of shoes during stance phase of running. Method: 47 male subjects (Age: 26.33 ± 2.11 years, Height: 177.32 ± 4.31 cm, Weight: 65.8 ± 3.87 kg) participated in this study. All subjects tested wearing the same type of running shoes by classifying bending stiffness (A shoes: 3.2~4.1 N, B shoes: 9.25~10.53 N, C shoes: 20.22~21.59 N). They ran 10 m at 3.3 m/s (SD ±3%) speed, and the speed was monitored by installing a speedometer at 3 m intervals between force plate, and the measured data were analyzed five times. During running, ankle joint, MTP joint, coupling angle, inclination angle (anterior-posterior, medial-lateral) was collected and analyzed. Vector coding methods were used to calculate vector angle of 2 joint couples during running: MTP-Ankle joint frontal plane. All analyses were performed with SPSS 21.0 and for repeated measured ANOVA and Post-hoc was Bonferroni. Results: Results indicated that there was an interaction between three shoes and phases for MTP (Metatarsalphalangeal) joint angle (p = .045), the phases in the three shoes showed difference with heel strike~impact peak (p1) (p = .000), impact peak~active peak (p2) (p = .002), from active peak to half the distance to take-off until take-off (p4) (p = .032) except for active peak~from active peak to half the distance to take-off (p3) (p = .155). ML IA (medial-lateral inclination angle) for C shoes was increased than other shoes. The coupling angle of ankle angle and MTP joint showed that there was significantly difference of p2 (p = .005), p4 (p = .045), and the characteristics of C shoes were that single-joint pattern (ankle-phase, MTP-phase) was shown in each phase. Conclusion: In conclusion, by wearing high bending stiffness shoes, their body instability was increased during running.

• Korean Journal of Metals and Materials
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• v.50 no.9
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• pp.685-690
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• 2012

Decalcomania is a new method for SOFCs (solid oxide fuel cells) unit cell fabrication. A tight and dense $5{\mu}m$ Yttria-stabilized zirconia (8YSZ) electrolyte layer on anode substrate was fabricated by the decalcomania method. After 8YSZ as the electrolyte starting material was calcined at $1200^{\circ}C$, the particle size was controlled by the attrition mill. The median particle size (D50) of each 8YSZ was $39.6{\mu}m$, $9.30{\mu}m$, $6.35{\mu}m$, and $3.16{\mu}m$, respectively. The anode substrate was coated with decalcomania papers which were made by using 8YSZ with different median particle sizes. In order to investigate the effect of median particle sizes and sintering conditions on the electrolyte density, each sample was sintered for 2, 5 and 10 h, respectively. 8YSZ with a median particle size of $3.16{\mu}m$ which was sintered at $1400^{\circ}C$ for 10 had the highest density. With this 8YSZ, a SOFCs unit cell was manufactured with a $5{\mu}m$ layer by the decalcomania method. Then the unit cell was run at $800^{\circ}C$. The Open Circuit Voltage (OCV) and Maximum power density (MPD) was 1.12 V and $650mW/cm^2$, respectively.

• The korean journal of orthodontics
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• v.51 no.3
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• pp.179-188
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• 2021

Objective: To evaluate the overall treatment effects in terms of the amount of uprighting with changes in the sagittal and vertical positions of mandibular molars after applying an orthodontic miniplate with a nickel-titanium (NiTi) reverse curve arch wire (biocreative reverse curve [BRC] system). Methods: A total of 30 female patients (mean age, 25.99 ± 8.96 years) were treated with the BRC system (mean BRC time, 10.3 ± 4.07 months). An I-shaped C-tube miniplate (Jin Biomed) was placed at the labial aspect for the alveolar bone of the mandibular incisors. A 0.017 × 0.025-inch NiTi reverse curve arch wire was engaged at the C-tube mini-plate anteriorly and the first and second premolars and molars posteriorly in the mandibular arch. Pre- and post-BRC lateral cephalograms were analyzed. A paired t-test was used to analyze the treatment effects of BRC. Results: The mandibular second molars were intrusively uprighted successfully by the BRC system. Distal uprighting with a controlled vertical dimension was noted on the first molars when they remained engaged in the BRC and the distal ends of the arch wire were laid on the second molars. The mandibular first and second premolars showed a slight extrusion. The changes in the mandibular incisors were unremarkable, while the mandibular molar angulation improved significantly. The lower occlusal plane rotated counterclockwise (MP-LOP: 1.13° ± 2.60°). Conclusions: The BRC system can provide very effective molar uprighting without compromising the position of the mandibular anterior teeth.

• Journal of the Korean Society of Food Culture
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• v.25 no.1
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• pp.61-69
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• 2010

This study was carried out to investigate the effects of various kinds of commercial salts, including sun-dried (Korea), purified, and traditional salts on the chemical and sensory properties and growth of microorganisms involved in kimchi fermentation. Kimchi was prepared by salting in 10% NaCl solution for 2 hours followed by addition of other spices and fermentation at $20^{\circ}C$. The decreases in pH suggested that kimchi fermentation can be classified into 3 steps: initial, intermediate, and final stages. In texture analysis, the hardness and fracturability of traditional salt kimchi were higher than those of regular kimchi. From the sensory evaluation test for kimchi, sensory scores were high for traditional salt addition, especially taste, overall preference and texture. Among various microorganisms related to kimchi fermentation, the growth of Leuconostoc mesenteroides, Lactobacillus plantarum, Pichia membranaefaciens and Escherichia coli were examined. Based on the conditions of kimchi fermentation, a 2% and 5% concentration of each salt were studied. Also, the conditions of the cultures at $37^{\circ}C$ were examined. There was no considerable difference in the growth of Leuconostoc mesenteroides, Lactobacillus plantarum, Escherichia coli in the different kinds of salts. However, the growth of Pichia membranaefaciens was strongly inhibited by a 5% concentration of traditional salt during incubation at $37^{\circ}C$.

• Research in Plant Disease
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• v.29 no.2
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• pp.174-183
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• 2023

The limited effectiveness of current plant disease management treatments necessitates the development of new methods for controlling diseases using beneficial microbes. Demanding sustainable agriculture is increasingly highlighted as a biocontrol approach, particularly Streptomyces species known to produce a variety of antibiotic compounds and secondary metabolites. The Streptomyces globisporus SP6C4 strain and Streptomyces sp. S8 have been reported as potent antifungal agents and are gaining attention for improving crop growth in sustainable agriculture. In this study, we investigated the use of Streptomyces species formulations to enhance bacterial growth with nitrogen sources. Specifically, the addition of L-glutamic acid and L-cysteine resulted in earlier sporulation and bacterial growth in Streptomyces strains, respectively. This approach could expand the range of fermentation techniques in agriculture and be useful for controlling plant growth-promoting bacteria.