• Title/Summary/Keyword: C sources

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Serotyping of Cryptococcus neoformans Strains Isolated in Korea

  • Hwang, Soo-Myung
    • Journal of Microbiology
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    • v.40 no.2
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    • pp.166-169
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    • 2002
  • Twenty strains of Cryptococcus neoformams isolated from environmental and clinical sources in Korea were examined for their serotypes. Two environmental isolates from pigeon excreta belonged to C. neoformans var. neoformans serotypes A. Of the 18 isolates from clinical specimens, 17 belonged to C. neoformans vats, neoiomans (serotype A : 16, serotype D : 1) and one belonged to C. neoformans vats, gattii serotype B, which was culturally unusual, producing mucous colonies. This is the first report of the identification of C. neoformans roar, gattii serotype B from a patient in Korea.

Preparation and Characterization of Various Chitin from Protunus Trituberculatus Shells Such As Waste Marine Sources (수산계 폐자원인 꽃게껍질로부터의 다양한 키틴의 제조)

  • 김종완;황성규;이기창;이한섭;박종주
    • Journal of Environmental Health Sciences
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    • v.24 no.3
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    • pp.11-17
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    • 1998
  • Chitin is known as biodegradable natural polymer. In spite of various application of chitin derivatives from waste marine sources, commercial use of chitin has been limited due to high resistance to chemicals and the absense of proper solvents. We chitin prepared through the decalcification, bleaching and deproteination from Protunus trituberculatus shells by change of Hackman's method. Also, Microcrystalline chitin made by hydrolysis that was reduce made of resistance solvents used by dilute hydrochloric acid, ultrasonic and hydrogen peroxide. Crosslinked chitin derivatives were preparaed from chitin with crosslink agents(epichlorohydrin, 1,3-dichloropropanol) follwed by crosslinkage at 6C position. The effects of these parameters on chitin dervatives were invastigated by IR, DSC, XRD, BET, PSA and SEM. SEM analysis showed that both chitin and crosslinked chitin had a particle shaped morphology.

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Induction of Glucoamylasen in the Yeast Candida tsukubaensis

  • Chun, Soon-Bai;Chung, Hee-Young
    • BMB Reports
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    • v.28 no.4
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    • pp.342-347
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    • 1995
  • The induction of glucoamylase biosynthesis from the yeast Candida tsukubaensis by different carbon sources was investigated by using either an enzyme activity assay or immunoblot analysis. The induction by C. tsukubaensis appears to be independent of the carbon sources, although the level of enzyme activity was lower in slowly utilizable carbon sources such as galactose. This glucoamylase is a constitutive enzyme and its biosynthesis is resistant to carbon catabolite repression. Glucose was more effective for the enzyme induction than starch, maltose or glycerol. In addition, this enzyme is regulated by both induction and repression.

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Cultural Characteristics of Ophiocordyceps heteropoda Collected from Korea

  • Sung, Gi-Ho;Shrestha, Bhushan;Han, Sang-Kuk;Sung, Jae-Mo
    • Mycobiology
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    • v.39 no.1
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    • pp.1-6
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    • 2011
  • Isolates of Ophiocordyceps heteropoda (Kobayasi) collected from Mt. Halla on Jeju-do, Korea were tested for mycelial growth on different agar media and in the presence of different carbon and nitrogen sources. Similarly, isolates were also incubated at different temperatures as well as under continuous light and dark conditions. Growth was better on Hamada agar, basal medium, and malt-yeast agar, but poor on Czapek-Dox agar. Different carbon sources such as dextrin, saccharose, starch, lactose, maltose, fructose, and dextrose resulted in better growth. Complex organic nitrogen sources such as yeast extract and peptone revealed the most effective growth. Mycelial growth was best at $25^{\circ}C$. The growth rate was faster in the dark than the light, but mycelial density was less compact in the dark.

Cellulase를 생산하는 Bacillus sp. 79-23 분리와 효소 생산성

  • 윤기홍;정경화;박승환
    • Microbiology and Biotechnology Letters
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    • v.25 no.6
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    • pp.546-551
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    • 1997
  • A bacterium producing the extracellular cellulases was isolated from soil and has been identified as Bacillus sp. The isolate, named Bacillus sp. 79-23, was shown to be very similar to B. subtilis on the basis of its biochemical properties. The carboxymethyl cellulase (CMCase) of culture supernatant was most active at 60$\circ$C and pH 6.0, and retained 90% of its maximum activity at pH 7.0. The additional carbon sources affected the CMCase productivity than nitrogen sources in the culture medium. The carbon sources including wheat bran, rice straw, maltose and glucose increased the enzyme productivty. Especially, the maximum CMCase production was 5.2 units/ml in LB medium supplemented with 3% (w/v) wheat bran, which was 13-folds more than that in LB medium. It was found that the enzyme production was in association with the growth of Bacillius sp. 79-23. But, whean bran did not affect the growth of isolate, suggesting that increasement of CMCase production was owing to the induction of CMCase biosynthesis by wheat bran. In addition, both water-soluble and insoluble components of wheat bran was involved in induction of CMCase biosynthesis.

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Production and Application of Sophorolipid, A Microbial Surfactant (소포로리피드[미생물계면활성제]의 생산과 응용)

  • Cho, Kwi-Joon;Kim, Young-Bum;Kim, Eun-Ki
    • KSBB Journal
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    • v.14 no.6
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    • pp.747-753
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    • 1999
  • Microbial surfactants are more effective and environmentally friendly than many synthetic surfactants. Sophorolipid, a glycolipid type microbial surfactant, is produced from C. bombiocola. Cultivation techniques to increase the productivity have been developed using various carbon sources and reactor setup, reaching its concentration upto 100-300 g/L. Due to its high productivity and non-toxicity, sophorolipid became one of the most promising alternative to synthetic surfactants. Fermentative production of sophorolipid depends primarily on the carbon sources, such as glucose and vegetable oils, and nitrogen sources. Chemical modification of the sophorolipid produces various derivative with different physical properties including hydrophile-liphophilie balance(HLB), emulsion formation, surface tension and dispersing ability. Commercial potentials of sophorolipid in the cosmetic, health care and environment clean-up industries have been discussed.

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AN EVALUATION OF RADIATION DOSES RESULTING FROM THE MEDICAL USE OF HIGH-ENERGY BETA-RAY SOURCES

  • Park, Jae-Woo;Kim, Hyun-Jo
    • Journal of Radiation Protection and Research
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    • v.26 no.3
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    • pp.149-154
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    • 2001
  • Calculational models to evaluate radiation doses resulting from the medical use of high energy beta-ray sources are presented. The radioactive sources considered are Sr-90/Y-90 used as ophthalmic applicator, Re-188 used for treating restenosis of coronary artery, and Ho-166 used for treating hepatic tumors. Typical therapeutic situations which might induce relatively high radiation doses the medical person involved were considered to compute by using MCNP-4C Monte Carlo code the radiation doses. The calculation results suggest that for all of the cases considered, the evaluated radiation doses are negligible compared to the dose limits. It is also found that the effect of Bremsstrahlung radiations on the total dose is insignificant, and hence the conventional lead gown is also effective in shielding beta-rays.

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Sources of Resistance to Bacterial Wilt Found in Vietnam Collections of Pepper (Capsicum annuum) and Their Nuclear Fertility Restorer Genotypes for Cytoplasmic Male Sterility

  • Tran, Ngoc Hung;Kim, Byung-Soo
    • The Plant Pathology Journal
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    • v.28 no.4
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    • pp.418-422
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    • 2012
  • Eleven hot pepper accessions collected in Vietnam showed stable resistance to bacterial wilt as well-known resistance sources, MC4 and MC5, in repeated inoculation tests with different Ralstonia solanacearum isolates conducted from 2004 to 2010. Seven of these accessions (specifically KC981, KC1006, KC1021, KC1027, KC1045, KC1050, and KC1055) resulted in stable male sterile F1 plants in the crosses with a cytoplasmically male sterile (CMS) Chilseong (CMS-A, Srfrf ), and therefore, they were maintainers (CMS-B) with a genotype of Nrfrf. The rest (KC980, KC995, KC999, and KC1009) produced stable male fertile F1 plants in the crosses, and therefore, were restorers (CMS-C) with a genotype of N(S)RfRf. Therefore, the maintainer and restorer sources of resistance may be used in preference in breeding maternal (CMS and their maintainers) and paternal parents (restorers) for resistance to bacterial wilt, respectively, in the hybrid breeding system utilizing cytoplasmic male sterility.

A comparing on the use of Centrifugal Turbine and Tesla Turbine in an application of Organic Rankine Cycle

  • Thawichsri, Kosart;nilnont, Wanich
    • International Journal of Advanced Culture Technology
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    • v.3 no.2
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    • pp.58-66
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    • 2015
  • This paper aims to compare the use of Centrifugal Turbine and Tesla Turbine in an application of Organic Rankine Cycle (ORC) Machine using Isopentane as working fluid expanding. The working fluid has boiling point below boiling water and works in low-temperature sources between $80-120^{\circ}C$ which can be produced from waste heat, solar-thermal energy and geothermal energy etc. The experiment on ORC machine reveals that the suitability of high pressure pump for working fluid has result on the efficiency of work. In addition, Thermodynamics theory on P-h diagram also presented the effect of heat sources' temperature and flow rate on any work. Thus, the study and design on ORC machine has to concern mainly on pressure pump, flow rate and optimized temperature. Result experiment and calculate ORC Machine using centrifugal Turbine efficiency better than Tesla turbine 30% but Tesla Turbine is cheaper and easily structure. Further study on the machine can be developed throughout the county due to its low cost and efficiency.