• 한국식품위생안전성학회지
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• 제14권4호
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• pp.327-332
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• 1999

1996년 3월부터 1998년 10월간에 경남지방 시판 냉장육 888건, 냉동포장육 222건 및 수입 냉동육 117건의 시료로부터 식중독관련 병원균의 분포와 혈청형을 조사하였다. 식중독균의 분리률은 Staphylocorcus aureus. Campylobarter jejuni/coli, Listeria monocytogenes 및 Salmonella spp 순으로 높았고, Escherichia coli O157:H7은 전시료를 통해서 분리되지 않았다. C. jejuni /coli는 냉장육에서 높은 오염률을 나타내고, 냉동포장육에서는 거의 분리되지 않는데 반해서 L. monocytogenes는 냉장육에 이해서 냉동포장 계육에서 높은 분리율을 나타낸 것은 매우 흥미로운 일이다. 분리균의 혈청형 분포는 Sta. aureus의 경우 대부분이 enterotoxin type C와 D였고, Salmonella spp는 돈육유래균에서 모두 A group이었으며, 계육유래균은 대부분 B와 D group이었다. L. monocytogenes는 계육유래균의 대부분이 type 1 이었고, type 4는 소수로 분리되었다.

• Journal of Microbiology and Biotechnology
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• 제22권11호
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• pp.1467-1470
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• 2012

We compared rapid fingerprinting using repetitive sequencebased PCR (rep-PCR) for subtyping Campylobacter jejuni isolates to the widely used multilocus sequence typing (MLST). Representative C. jejuni isolates (n = 16) from broilers were analyzed using MLST and rep-PCR. Both techniques demonstrated an equal discriminatory power of 0.8917, and 9 subgroups were identified. Clonal identification of all 16 isolates was identical for both techniques. The rep-PCR as described in this study may be used as a rapid and cost-effective alternative for subtyping of C. jejuni isolates, or as an effective screening tool in large epidemiological studies.

• 대한수의학회지
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• 제27권2호
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• pp.245-251
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• 1987

A total of 145 strains of thermophilic Campylobacter spp. isolated from the fecal specimens of 108 cattle, 120 pigs and 104 chickens. The isolation rates of Campylobacter jejuni from cattle, pigs and chickens were 36.1%, 38.3% and 28.8%, respectively. In the biotyping of 115 strains of C. jejuni, 49.6% were belonged to biotype I, 33.9% biotype II, 10.4% biotype IV and 6.1 % biotype III. Twenty-eight strains of C. coli were 78.6% of biotype I, 21.4% biotype II. Two strains of C. laridis belonged to biotype I and II. One hundred of 105 C. jejuni cultures were typable serologically and represented 13 serogroups Serotype 4, 5, 26, 27 and 36 were encountered most frequently. Eighteen of 23 C. coli cultures were typable serologically and represented 6 serogroups. Serotype 8, 20, 21 and 31 were encountered most frequently. In the comparison of frequency of serotype between animal species, serotypes 4, 30, 5, 26 and 27 were encountered relatively common in the cattle source isolates, serotypes 26 and 36 in the pigs, and 36 and 17 in the chickens. The serotypes of C. coli encountered most frequently were serotype 8 and 31.

• Preventive Nutrition and Food Science
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• 제12권4호
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• pp.279-283
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• 2007

Aqueous chlorine dioxide $(ClO_2)$ treatment was used for the inactivation of Listeria monocytogenes and Campylobacter jejuni in chicken. Chicken breasts and legs were inoculated with $8{\sim}9log\;CFU/g$ of Listeria monocytogenes and Campylobacter jejuni, respectively, and then treated with 0, 50, and 100 ppm of $ClO_2$ solution. Aqueous $ClO_2$ treatment decreased the populations of the pathogenic bacteria on the chicken samples. One hundred ppm $ClO_2$ treatment on the chicken breast and leg reduced the populations of Listeria monocytogenes and Campylobacter jejuni by $0.61{\sim}1.93\;and\;0.99{\sim}1.21log\;CFU/g$, respectively. Aqueous $ClO_2$ treatment affected the microbial growth during storage at $4^{\circ}C$ by decreasing the initial microbial populations. These results clearly suggest that aqueous $ClO_2$ treatment should be useful in improving the microbial safety of chicken during storage and extending the shelf life.

• 대한수의학회지
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• 제29권3호
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• pp.303-313
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• 1989

To investigate the epidemiological trait of intestinal diseases of animals caused by thermophilic Campyllobacter spp., isolation of etiological agent was carried out and the profiles of plasmids and the transfer of resistance plasmid in the isolated Campylobacter spp. were examined. The results were as follows. 1. A total of 110 isolates of C jejuni and C coli were subjected to the test for the presence of plasmid DNA. Of the isolates examined, 60% of the isolates were noted to harbor plasmid DNA. Plasmid occurrencer ate from pigs, chickens and cattle were 76.2%, 61.7% and 37.7%, respectively. The plasmids of a large molecular weight, ranging from 36 Md to 86Md, were identified with the strains of tetracycline resistant. 2. Transfer frequency of tetracycline resistant plasmids was higher in the case of the filter mating method than in the broth mating method by the factor of 10~1,000. 3. Tetracycline resistant plasmids of C jejuni were transferrable to C jejuni and C coli by conjugation. In a low frequency, the transfer of tetracycline plasmid was also possible to Vibrio parahemolyticus. However, it was impossible to transfer to Streptococcus fecalis, E coli and Vibrio cholerae. 4. Tetracycline resistant plasmids of C jejuni were impossible to transfer to Campylobacter spp. and related bacteria by transformation.

• Bulletin of the Korean Chemical Society
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• 제25권2호
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• pp.289-292
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• 2004

A trisaccharide, GlcNAcp- ${\beta}-(1{\to}3)-Galp-{\alpha}-(1{\to}2)$-6-deoxy-altroHepp- ${\alpha}-(1{\to}O$-propyl, as an O-antigenic repeating unit of C. jejuni serotype O:23 and O:36 was synthesized. Coupling of the GlcNPhth-(1${\to}$3)-Gal disaccharide donor with allyl 6-deoxy-altroHep acceptor in the presence of iodonium dicollidine perchlorate (IDCP) promoter afforded the ${\alpha}$-galactosidic trisaccharide with high stereoselectivities. Subsequent deacetalation, dephthaloylation, N-acetylation, and hydrogenolytic debenzylation furnished the title compound.

• Archives of Pharmacal Research
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• 제27권2호
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• pp.143-150
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• 2004

A trisaccharide, the O-antigenic repeating unit of C. jejuni serotype O:23 and O:36, was synthesized as a 2 -azidoethyl glycoside by block addition of perbenzylated thiogalactoside donors to $\alpha$-altroHepp-(1$\rightarrow$3)-GlcNPhth disaccharide acceptor in presence of IDCP promoter. The $\alpha$-linked altroheptopyranoside moiety in the glycosyl acceptor was effectively prepared by Swern oxidation of $\alpha$-mannohepp-(1$\rightarrow$3)-GlcNPhth disaccharide followed by mild reduction with1 $NaCNBH_3$.

• 미생물학회지
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• 제54권1호
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• pp.31-37
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• 2018

캄필로박터 제주니 균(Campylobacter jejuni)은 세균성위장관감염증을 일으키는 수인성식품매개질환의 중요한 원인 균으로 알려져 있다. 2014년부터 2016년까지 경기지역에서 발생된 17번의 식중독에서 캄필로박터 제주니 균에 감염된 430명의 환자와 조리종사자에게서 208건의 균주를 선별하였다. 선별된 균주의 유전적 상관관계와 유전형분포를 확인하기 위하여 PFGE와 multiplex-PCR typing 방법을 사용하여 비교분석 하였다. 47개의 Penner-type으로 구분되는 캄필로박터 제주니 균의 혈청형을 multiplex-PCR typing을 이용하여 35개의 유전형으로 구분할 수 있는 것을 확인하였고 선별된 균주에서 7개의 유전형(HS2, HS4A, HS8, HS15, HS29, HS41, HS53)으로 구분되는 것을 확인하였다. 가장 많은 케이스에서 분리된 유전형은 HS2였고 7건의 식중독케이스에서 확인되었다. PFGE를 통하여 11건의 식중독에서 모두 5개의 그룹으로 분류되었고 그룹간의 유사성은 61.8에서 66.6%였다. 본 연구는 다양한 유전자 분석방법을 통하여 경기도내에서 분리된 캄필로박터 제주니 균의 유전적 다양성을 파악하고 향후 집단발생시 환자의 분리 균주 간의 상관관계 규명하며 캄필로박터 감염증의 발생 및 확산 방지에 필요한 기초자료를 마련하고자 한다.

• 한국동물위생학회지
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• 제38권3호
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• pp.167-171
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• 2015

Pathogenic microorganisms were monitored on the chicken carcasses in slaughterhouse of Jeonbuk area. It was conducted to evaluate the microbiological quality on 204 chicken carcasses. Staphylococcus (S.) aureus was isolated in largest number and its ration was 41.2%, Salmonella spp. 6.4%, Campylobacter (C.)jejuni 7.4%, C. coli 7.4%. Serotype of Salmonella (S.) spp. was identified as S. Infantis 46.1%, S. Enteritidis 23.1%, S. Typhimurium 7.7%, S. Montevideo 7.7%. In breed chickens, Salmonella spp. was detected broiler 4.1%, white semi-broiler 8.0% Korean native chicken 12.0%. C. jejuni was isolated broiler 7.4%, white semi-broiler 12.0%, Korean native chicken 0%, C. coli, broiler 7.4%, white semi-broiler 0%, Korean native chicken 18.1% and S. aureus, broiler 38.8%, white semi-broiler 40.0%, Korean native chicken 51.5%.

• 대한의생명과학회지
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• 제18권2호
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• pp.152-159
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• 2012

Campylobacter species are known to the high optimum growth temperature ($42^{\circ}C$) and the cause of enteritis in people. Erythromycin has a curative effect for enteritis caused by the bacteria. However, the rate of erythromycin-resistant bacteria was not well known until recently in Korea. Swine are one of sources of the infection with a Campylobacter species which cause the symptom of a high temperature. In this study, we cultured rectum fecal specimens of 100 pigs in an area of Buan-gun, Jeonbuk Province during July 2009. As a result, the detection rate of C. jejuni and C. coli and the rate of erythromycin-resistant bacteria for the separated Campylobacter species on the condition of high temperature were investigated. The possession or not of hipO and glyA gene and ciprofloxacin-resistant gene gyrA was also reviewed with biochemical characteristics and PCR.